Product Datasheet. IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) NB Unit Size: 0.1 mg

Transcription

1 Product Datasheet IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) NB Unit Size: 0.1 mg Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Publications: 11 Protocols, Publications, Related Products, Reviews, Research Tools and Images at: Updated 8/22/2018 v.20.1 Earn rewards for product reviews and publications. Submit a publication at Submit a review at

2 NB IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) Product Information Unit Size Concentration Storage Clonality Clone Preservative Isotype Purity Buffer Product Description Host 0.1 mg 1.0 mg/ml Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Monoclonal 39A % Sodium Azide IgG1 Kappa Protein G purified PBS Mouse Gene ID 4792 Gene Symbol Species Specificity/Sensitivity Immunogen NFKBIA Human, Mouse, Rat, Porcine, Bovine, Canine IkBa (39A1413 ) [Ser32/Ser36] Page 1 of 5 v.20.1 Updated 8/22/2018 This monoclonal antibody was raised against a synthetic peptide containing phosphorylated serines at amino acid residues 32 and 36 of human IkBa. Product Application Details Applications Recommended Dilutions Western Blot, Immunoprecipitation Western Blot 1-2 ug/ml, Immunoprecipitation 1-2 ug/ml

3 Images Western Blot: IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) [NB ] - Analysis using the Biotin conjugate of NB Detection of Jurkat cells were treated for 30 min with 100 ug/ml ALLN (N-Acetyl- Leu-Leu-Norleucinal; a Calpain inhibitor and also proteasome inhibitor that prevents IkBa dephosphorylation) followed by incubation with (lanes 2 & 4) or without 1 nm TNF-a (1 & 3). The membranes were blotted with this antibody. Page 2 of 5 v.20.1 Updated 8/22/2018 Western Blot: IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) [NB ] - Jurkat cells were treated for 30 min with 100 ug/ml ALLN (N- Acetyl-Leu-Leu-Norleucinal) - a Calpain inhibitor and also proteasome inhibitor that prevents IkBa dephosphorylation) followed by incubation with (lane 2) or without 1 nm TNF-a (lane 1). The membrane was blotted with NB and immunoreactivity was detected by ECL. The data shows that NB detects specifically the phosphorylated form of IkBa.

4 Publications Kanno Y, Maruyama C, Matsuda A, Ishisaki A. upa-derived peptide, A6 is involved in the suppression of lipopolysaccaride-promoted inflammatory osteoclastogenesis and the resultant bone loss. Immun Inflamm Dis May :00AM [PMID: ] (WB, Mouse) Gu LX, Wen ZS, Xiang XW et al. Immunomodulatory effect of low molecular-weight seleno-aminopolysaccharides in intestinal epithelial cells. Int. J. Biol. Macromol. Mar :00AM [PMID: ] (WB, Porcine) Lotz Sonja, Aga Eresso, Wilde Inga et al. Highly purified lipoteichoic acid activates neutrophil granulocytes and delays their spontaneous apoptosis via CD14 and TLR2. J Leukoc Biol Mar [PMID: ] Shakibaei Mehdi, Allaway David, Nebrich Simone, Mobasheri Ali. Botanical Extracts from Rosehip (Rosa canina), Willow Bark (Salix alba), and Nettle Leaf (Urtica dioica) Suppress IL-1beta-Induced NF-kappaB Activation in Canine Articular Chondrocytes. Evid Based Complement Alternat Med [PMID: ] Samanta D, Gilkes D, Chaturvedi P, Xiang L, Semenza G. Hypoxia-inducible factors are required for chemotherapy resistance of breast cancer stem cells. Proc Natl Acad Sci U S A Dec 1 [PMID: ] (WB, Human) Das U, Manna K, Sinha M et al. Role of ferulic Acid in the amelioration of ionizing radiation induced inflammation: a murine model. PLoS ONE May 23 [PMID: ] (WB, Mouse) Liver, Fig 8. Jazirehi AR, Huerta-Yepez S, Cheng G et al. Rituximab (chimeric anti-cd20 monoclonal antibody) inhibits the constitutive nuclear factor-{kappa}b signaling pathway in non-hodgkin's lymphoma B-cell lines: role in sensitization to chemotherapeutic drug-induced apoptosis. Cancer Res Jan 1 [PMID: ] (WB) WB (Ramos and Daudi cells growing in RPMI 1640 and 1% fetal bovine serum and rituximab), Fig. 4a Ikezoe T, Yang Y, Heber D et al. PC-SPES: a potent inhibitor of nuclear factor-kappa B rescues mice from lipopolysaccharide-induced septic shock. Mol Pharmacol Dec [PMID: ] Han HS, Karabiyikoglu M, Kelly S et al. Mild hypothermia inhibits nuclear factor-kappab translocation in experimental stroke. J Cereb Blood Flow Metab May [PMID: ] Li Q, Guo Y, Tan W et al. Cardioprotection afforded by inducible nitric oxide synthase gene therapy is mediated by cyclooxygenase-2 via a nuclear factor-kappab dependent pathway. Circulation Oct 2 [PMID: ] (WB, Mouse) WB (WT, COX-2-/-, IkBa (Ser 32A,Ser 36A) Tg mice injected with inos gene), Fig. 5. Page 3 of 5 v.20.1 Updated 8/22/2018 Yang M, Hase H, Legarda-Addison D et al. B cell maturation antigen, the receptor for a proliferation-inducing ligand and B cell-activating factor of the TNF family, induces antigen presentation in B cells. J Immunol Sep 1 [PMID: ] (WB) Antibodies cited [WB]: 1. IkBa Ser 32/Ser 36 [IMG-156A], (A20 B cells stimulated with APRIL), Fig. 2c 2. IkBa [IMG-127A], (A20 cells stimulated with anti-tnfr2), Fig. 6a

5 Novus Biologicals USA 8100 Southpark Way, A-8 Littleton, CO USA Phone: Toll Free: Fax: Novus Biologicals Canada 461 North Service Road West, Unit B37 Oakville, ON L6M 2V5 Canada Phone: Toll Free: Fax: Novus Biologicals Europe 19 Barton Lane Abingdon Science Park Abingdon, OX14 3NB, United Kingdom Phone: (44) (0) Free Phone: Fax: (44) (0) General Contact Information Technical Support: Orders: General: Products Related to NB HAF007 NB720-B NBP mg NB Goat anti-mouse IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Rabbit anti-mouse IgG (H+L) Secondary Antibody [Biotin] (Preadsorbed) Mouse IgG1 Kappa Light Chain Isotype Control (P ) IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) [Biotin] Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt. For more information on our 100% guarantee, please visit Earn gift cards/discounts by submitting a review: Earn gift cards/discounts by submitting a publication using this product:

6