Product Datasheet. KAP1 Antibody NB Unit Size: 100 ul. Store at 4C. Do not freeze. Publications: 16

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1 Product Datasheet KAP1 Antibody NB Unit Size: 100 ul Store at 4C. Do not freeze. Publications: 16 Protocols, Publications, Related Products, Reviews, Research Tools and Images at: Updated 6/18/2018 v.20.1 Earn rewards for product reviews and publications. Submit a publication at Submit a review at

2 NB KAP1 Antibody Product Information Unit Size Concentration Storage Clonality Preservative Isotype Purity 100 ul 1.0 mg/ml Store at 4C. Do not freeze. Polyclonal 0.09% Sodium Azide IgG Immunogen affinity purified Buffer Tris-Citrate/Phosphate (ph ) Page 1 of 4 v.20.1 Updated 6/18/2018 Product Description Host Rabbit Gene ID Gene Symbol Species TRIM28 Human, Mouse Immunogen The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human Tripartite Motif-Containing 28 using the numbering given in entry NP_ (GeneID 10155). Notes Product Application Details Applications This anitbody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:nb , NB , NB Western Blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation Recommended Dilutions Western Blot 1:1000-1:20000, Immunohistochemistry 1:1000-1:5000, Immunocytochemistry/Immunofluorescence 1:250-1:1000, Immunoprecipitation 1-4 ug/mg of lysate, Immunohistochemistry-Paraffin 1:1000-1:5000 Application Notes Images Immunohistochemistry 1:500 to 1:2,000. Epitope retrieval with citrate buffer ph6.0 is recommended for FFPE tissue sections. Immunocytochemistry 1:500 to 1:2,500. Permeabilization with Triton-X 100 is recommended for formaldehydefixed cells. Western Blot: KAP1 Antibody [NB ] - Detection of Human KAP-1 by Western Blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug), and 293T (50ug) cells. Antibodies: Affinity purified rabbit anti-kap-1 antibody NB used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

3 Immunohistochemistry: KAP1 Antibody [NB ] - Samples: FFPE sections of human breast carcinoma. Antibody: Affinity purified rabbit anti-kap-1 Cat. No. Lot3 (left) and Lot2 (right) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Page 2 of 4 v.20.1 Updated 6/18/2018 Western Blot: KAP1 Antibody [NB ] - Whole cell lysate from HeLa (5, 15, 50 ug), and 293T (50ug) cells. Western Blot: KAP1 Antibody [NB ] - Detection of Human KAP-1 on HeLa whole cell lysate using NB Immunoprecipitated KAP-1 was detected using a KAP-1 antibody from another source. Immunohistochemistry-Paraffin: KAP1 Antibody [NB ] - FFPE section of human skin tumor. Affinity purified rabbit anti-kap-1 used at a dilution of 1:100.

4 Immunohistochemistry-Paraffin: KAP1 Antibody [NB ] - Human prostate carcinoma (left) and formaldelyde-fixed HeLa cells (right). Antibody used at a dilution of 1:1,000. (left) and green fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody FITC conjugated used at a dilution of 1:100 (right). Page 3 of 4 v.20.1 Updated 6/18/2018 Publications Batenburg NL, Thompson EL, Hendrickson EA, Zhu XD. Cockayne syndrome group B protein regulates DNA doublestrand break repair and checkpoint activation. EMBO J Mar 27 [PMID: ] (WB, Mouse) Nakamura K, Fike F, Haghayegh S. A-TWinnipeg: Pathogenesis of rare ATM missense mutation c.6200c>a with decreased protein expression and downstream signaling, early-onset dystonia, cancer, and life-threatening radiotoxicity. Molecular Genetics & Genomic Medicine 2/2/2014 (WB, Human) Puccini J, Shalini S, Voss AK et al. Loss of caspase-2 augments lymphomagenesis and enhances genomic instability in Atm-deficient mice. Proc Natl Acad Sci U S A 2013 Dec 3 [PMID: ] (WB, Mouse) Narayan V, Halada P, Hernychova L et al. A multiprotein binding interface in an intrinsically disordered region of the tumor suppressor protein interferon regulatory factor-1. J Biol Chem 2011 Apr [PMID: ] Gilmore-Hebert M, Ramabhadran R, Stern DF et al. Interactions of ErbB4 and Kap1 connect the growth factor and DNA damage response pathways. Mol Cancer Res 2010 Oct [PMID: ] Briers S, Crawford C, Bickmore WA et al. KRAB zinc-finger proteins localise to novel KAP1-containing foci that are adjacent to PML nuclear bodies. J Cell Sci 2009 Apr [PMID: ] Tsuruma R, Ohbayashi N, Kamitani S et al. Physical and functional interactions between STAT3 and KAP1. Oncogene 2008 May [PMID: ] Wang C, Rauscher FJ 3rd, Cress WD et al. Regulation of E2F1 function by the nuclear corepressor KAP1. J Biol Chem 2007 Oct [PMID: ] Ziv Y, Bielopolski D, Galanty Y et al. Chromatin relaxation in response to DNA double-strand breaks is modulated by a novel ATM- and KAP-1 dependent pathway. Nat Cell Biol 2006 Aug [PMID: ] Wang C, Ivanov A, Chen L et al. MDM2 interaction with nuclear corepressor KAP1 contributes to p53 inactivation. EMBO J 2005 Sep [PMID: ] Yang Y, Fiskus W, Yong B et al. Acetylated hsp70 and KAP1-mediated Vps34 SUMOylation is required for autophagosome creation in autophagy. Proc Natl Acad Sci U S A 2013 Apr 23 [PMID: ] (WB, ICC/IF, IP, Human) Bhatia N, Xiao TZ, Rosenthal KA et al. MAGE-C2 Promotes Growth and Tumorigenicity of Melanoma Cells, Phosphorylation of KAP1, and DNA Damage Repair J Invest Dermatol 2012 Oct 25 [PMID: ] (WB, Human) More publications at

5 Novus Biologicals USA E. Briarwood Avenue Centennial, CO USA Phone: Toll Free: Fax: Novus Biologicals Canada 461 North Service Road West, Unit B37 Oakville, ON L6M 2V5 Canada Phone: Toll Free: Fax: Novus Biologicals Europe 19 Barton Lane Abingdon Science Park Abingdon, OX14 3NB, United Kingdom Phone: (44) (0) Free Phone: Fax: (44) (0) General Contact Information Technical Support: Orders: General: Products Related to NB HAF008 NB7156 NBP H P01-10ug Goat anti-rabbit IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Goat anti-rabbit IgG (H+L) Secondary Antibody Rabbit IgG Isotype Control Recombinant Human KAP1 Protein Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt. For more information on our 100% guarantee, please visit Earn gift cards/discounts by submitting a review: Earn gift cards/discounts by submitting a publication using this product: