Curriculum Vitae. Year Degree Institution Major 1991 B.S. Drake University Biology M.A. Drake University Biology

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1 Name: Campus Address: Sean Patrick Didion, PhD, FAHA The University of Mississippi Medical Center Departments of Pharmacology and Neurology Arthur C. Guyton Laboratory Research Building 2500 North State Street Jackson, Mississippi Phone: (601) Marital Status: Married: Lisa Ann Didion, MD, FAAP Associate Professor & Chief Medical Officer Director, Office of Patient Satisfaction Batson Children s Hospital Department of Pediatrics University of Mississippi Medical Center Jackson, Mississippi Children: Riley Ann, 13 years Andrew James, 11 years Colin Patrick, 8 years Mairead Emma, 7 years EDUCATION: Year Degree Institution Major 1991 B.S. Drake University Biology 1994 M.A. Drake University Biology 1998 Ph.D. University of Nebraska Molecular and Cellular Physiology

2 ACADEMIC POSITIONS: Post-doctoral Fellow, Department of Internal Medicine, Division of Cardiovascular Medicine, The University of Iowa Assistant Research Scientist, Department of Internal Medicine, Division of Cardiovascular Medicine, The University of Iowa Associate Research Scientist, Department of Internal Medicine, Division of Cardiovascular Medicine, The University of Iowa Research Scientist, Department of Internal Medicine, Division of Cardiovascular Medicine, The University of Iowa Assistant Professor, Department of Neurology and The Vascular Biology Center, Medical College of Georgia Associate Professor, Departments of Pharmacology and Neurology, The University of Mississippi Medical Center Associate Director, Department of Pharmacology Graduate Program, The University of Mississippi Medical Center Director, MD/PhD Program, The University of Mississippi Medical Center Associate Professor with Tenure, Departments of Pharmacology and Neurology, The University of Mississippi Medical Center Brief Biography: Dr. Sean P. Didion, Ph.D. is an Associate Professor in the Departments of Pharmacology and Neurology at the University of Mississippi Medical Center (UMMC) in Jackson, MS. He received his B.S. and M.A. degrees in Biological Sciences from Drake University and his Ph.D. degree in Physiology under the mentorship of Dr. William G. Mayhan, PhD in the department of Cellular and Page 2

3 Molecular Physiology (chaired by Dr. Irving H. Zucker, PhD) from The University of Nebraska Medical Center. His postdoctoral training was conducted at the University of Iowa Carver College of Medicine under the mentorship of Dr. Frank M. Faraci, PhD. Dr. Didion's current research is focused on understanding mechanisms by oxidative stress and inflammatory molecules play in the impairment of vascular function in development of obesity and hypertension. Dr. Didion has maintained an uninterrupted track record of funding as a Principal Investigator, which has totaled nearly $9 million (approx. $3 million as Principal Investigator) to date. Dr. Didion's work is currently funded by 2 R01's from the National Institutes of Health. In recognition of his research, he received the UMMC Gold Medal for Excellence in Research in He is an active member of the APS, having served on the Membership Committee and Perkins Memorial Award Committees. Dr. Didion has served on the Editorial Boards of the leading cardiovascular journals in his field, including, Atherosclerosis, Thrombosis, and Vascular Biology, Circulation Research, and Hypertension. Dr. Didion is a 2014 graduate of the UMMC Leadership Development Program and is the director of the UMMC MD/PhD Program. Dr. Didion's wife, Dr. Lisa A. Didion, MD, is an Associate Professor in the Departmen of Pediatrics and Associate Chief Medical Officer of Children's of Mississippi. They have 4 children and currently live in Madison, MS. Teaching and Education: Evaluation of Dr. Didion's course directorship and teaching in Medical, Dental, and Graduate Pharmacology courses has been extremely positive. Dr. Didion has also been recognized for his efforts to raise-the-bar by recruiting nationally recognized speakers to the Pharmacology Seminar Series and he was instrumental in ensuring that the departmental seminar series meet on a weekly basis. In addition, Dr. Didion established a weekly Journal Club series to the Department of Pharmacology thereby bringing faculty, students and trainees together to discuss the most recent advances, topics and papers in the field of Pharmacology. Dr. Didion worked to ensure that faculty as part of their education evaluated students and trainees. Dr. Didion has also worked to formalize many of the educational components Page 3

4 related to the MD-PhD program, all of which has been well received by students and faculty alike. Administrative Expertise and Interests: Dr. Didion fulfills a number of servicerelated roles both at the departmental and institutional levels, but also at the national and international level with his involvement in the National Association of MD-PhD Directors and the AAMC GREAT Group. Dr. Didion is working toward developing innovative methods for the education and training of physician-scientists and looks to bring his ideas to the national arena, as training and development of future physicianscientists is one of Dr. Didion s passions. Services and Procedures: Dr. Didion is currently the Director of Histological Services for the Department of Pharmacology. Services provided by Dr. Didion include, tissue processing, sectioning of paraffin embedded tissues as well as fixation of fresh frozen tissues. Dr. Didion provides routine histological staining of paraffin-fixed tissues, including H&E, Masson s Tri-chrome, PAS. Services also provided by Dr. Didion included specialized immunohistochemistry techniques. Dr. Didion also provides image and data analysis and quantification. This is a major service that Dr. Didion provides to the Department of Pharmacology and it is estimated that as of January 2016 to date Dr. Didion has processed well over 5,000+ tissue samples and more than 12,000+ individual slides for departmental faculty. AWARDS & HONORS: American Heart Association Pre-Doctoral Fellowship 1996 Procter & Gamble Professional Opportunity Award, Cardiovascular Section, American Physiological Society st Place Basic Science Oral Presentation, Student Research Forum, University of Nebraska Medical Center Blanche Widaman Fellowship & Regents Tuition Fellowship, University of Nebraska Medical Center Page 4

5 1998 Alice Cummings Prize in Physiology (Top Physiology Student Award), University of Nebraska Medical Center Institutional Research Fellowship, The University of Iowa, National Institutes of Health (HL ) Individual National Research Service Award, National Institutes of Health (HL ) Scientist Development Award, American Heart Association, National Affiliate 2002 Young Investigator Award, International Society of Hypertension 2002 Merck New Investigator Award, High Blood Pressure Council, American Heart Association 2006 Young Scholar Award, American Society of Hypertension 2009 Recognized as a Superior Editorial Consultant for Circulation Research 2012 Fellow of the American Heart Association, High Blood Pressure Council 2012 Gold Medal in Research Excellence Award, University of Mississippi Medical Center Year Service Award, National Association of MD-PhD Programs PROFESSIONAL SOCIETIES: American Association for the Advancement of Science American Physiological Society Member, Stroke Council, American Heart Association Page 5

6 2001- Member, Council on Basic Cardiovascular Sciences, American Heart Association Member, High Blood Pressure Council, American Heart Association Member, North American Vascular Biology Organization American Society of Hypertension Member, American Society for Pharmacology and Experimental Therapeutics Member, Society for Free Radical Biology and Medicine Life Member, Mississippi Academy of Sciences Member, American Association of University Professors PROFESSIONAL SERVICE: National Peer Review, Vascular Biology and Blood Pressure Regulation Committee, American Heart Association Medical College of Georgia, Vascular Biology Center Post-Doctoral Fellow Review Committee Member Member, American Physiological Society, Membership Committee Member Member, National American Heart Association Vascular Biology and Blood Pressure Regulation Study Section Member, American Physiological Society, Perkins Memorial Fund Committee 2015 Ad Hoc Member, Diabetes United Kingdom, Grant Review Committee Page 6

7 2015 Member, Cardiovascular Health One, National Peer Reviewed Medical Research Program, Department of Defense Congressionally Directed Medical Research Programs Member, Vascular Malformations, National Peer Reviewed Medical Research Program, Department of Defense Congressionally Directed Medical Research Programs EDITORIAL BOARDS: Circulation Research Hypertension Frontiers in Physiology - Oxidant Physiology Atherosclerosis, Thrombosis, and Vascular Biology American Journal of Cardiovascular Disease Frontiers in Physiology - Cardiac Electrophysiology Journal of Hypertension AD HOC REVIEWER FOR JOURNALS: American Journal of Physiology Heart and Circulatory Physiology American Journal of Physiology Regulatory and Integrative Physiology Antioxidants and Redox Signaling Atherosclerosis, Thrombosis and Vascular Biology (ATVB) Basic & Clinical Pharmacology & Toxicology Biochimica et Biophysica Acta Molecular Basis of Disease Biological Research for Nursing Brain Research Page 7

8 British Journal of Pharmacology Circulation Circulation Research Coronary Artery Disease Current Medicinal Chemistry Diabetes Diabetes Care Diabetic Medicine FASEB Journal FEBS Letters Free Radical Biology and Medicine Frontiers: Cardiovascular Medicine Hypertension Journal of the American Heart Association Journal of Applied Physiology Journal of Hypertension Journal of Hypertension Research Journal of Molecular Histology Life Sciences Microcirculation Microvascular Research Molecular Neurobiology Pharmacological Research Public Library of Science (PLoS) One Page 8

9 Stroke Translational Research Vascular Pharmacology INVITED LECTURES: 2004 Department of Pathology, Vascular Protection: Role of Superoxide Dismutase and PPAR-gamma, Weill Medical College, Cornell University, New York, New York 2004 Department of Cellular and Molecular Physiology, Mechanisms of Oxidative Stress and Protection in the Vasculature, University of North Carolina, Chapel Hill, North Carolina 2004 Department of Physiology, Oxidative Stress and Vascular Function, University of Mississippi Medical Center, Jackson, Mississippi 2004 Department of Physiology, Role of CuZn-Superoxide Dismutase in Vascular Protection, West Virginia University, Morgantown, West Virginia 2006 Winter Conference on Brain Research, Super Size Me: Vascular Responses in the Cerebral Circulation in Obesity and Type II Diabetes, Steamboat Springs, Colorado 2006 American Society of Hypertension, Young Scholar Award Lecture, Vascular Dysfunction in Obesity and Type II Diabetes: New Perspectives from Genetic Models, New York, New York Page 9

10 2006 Neuroscience Program, Role of Superoxide Dismutases in Limiting Cerebral Vascular Dysfunction, Michigan State University, East Lansing, Michigan 2006 Department of Neurology Grand Rounds, Role of Superoxide Dismutases in Limiting Cerebral Vascular Dysfunction, University of Iowa Hospital and Clinics, Iowa City, Iowa rd International Symposium on Cerebral Blood Flow and Metabolism, The Role of Oxidative Stress in the Cerebral Circulation, Osaka, Japan 2007 Center for Cardiovascular Sciences, Oxidative and Inflammatory Mechanisms Associated with Cerebral Vascular Dysfunction, Albany Medical College, Albany, New York 2007 Iowa and Nebraska Physiology Societies Combined Meeting, Genetic Deficiency of Interleukin-6 Protects Against Angiotensin II Induced Endothelial Dysfunction and Hypertrophy, Nebraska City, Nebraska 2007 Vascular Biology Center, Oxidative and Inflammatory Mechanisms Associated with Cerebral Vascular Dysfunction, Medical College of Georgia, Augusta, Georgia 2008 Department of Cellular and Molecular Physiology, Oxidative Stress, Inflammation, and Angiotensin Too, University of Nebraska Medical Center, Omaha, NE 2010 Vascular Biology Center, Stress, Strain, and Angiotensin II: Let s Rho ll, Medical College of Georgia, Augusta, GA 2010 Department of Pharmacology, Inflammatory Mechanisms of Aging and Hypertension, University of Mississippi Medical Center, Jackson, MS Page 10

11 2010 Department of Cellular and Molecular Physiology, Inflammatory Mechanisms of Hypertension, Loyola University Stritch School of Medicine, Maywood, IL 2010 Department of Medicine, Division of Cardiovascular Medicine, Inflammatory Mechanisms of Endothelial Dysfunction: Insights from IL-6 and IL-10, University of Cincinnati, Cincinnati, OH 2011 Department of Health and Human Physiology, Vascular Dysfunction in Aging and Hypertension: A Pro-Inflammatory Role for Interleukin-6, The University of Iowa, Iowa City, IA 2013 Department of Physiology, Vascular Phenotypes and Whole Genome Profiles in Obesity: The Mirrored Maze of Mouse Models, The University of Mississippi Medical Center, Jackson, MS 2014 Department of Pharmacology, Hypertension, Endothelial Dysfunction and Angiotensin Too, The University of Mississippi Medical Center, Jackson, MS 2014 Department of Physiology, Inflammation, Endothelial Dysfunction and Angiotensin II, Tulane University, New Orleans, LA 2016 Center for Cardiovascular and Renal Research, Novel Genetic Mouse Model of Hypertension, The University of Mississippi Medical Center, Jackson, MS 2016 AAMC GREAT and GRAND Group Meeting, Shaping the Future of the Physician-Scientist Workforce: Enhancing Pre-doctoral Fellowship Success Rates through a Proactive and Team-based Approach, Chicago, IL Page 11

12 TEACHING ACTIVITIES: (Classroom, Laboratory and Journal Club) Laboratory Instructor, Biology I for Majors, Drake University, Des Moines, Iowa Laboratory Instructor, Biology II for Majors, Drake University, Des Moines, Iowa Laboratory Instructor, Comparative Anatomy, Drake University, Des Moines, Iowa Cardiovascular Journal Club, Department of Physiology, University of Nebraska Medical Center, Omaha, Nebraska Neuroscience Journal Club, Department of Pharmacology, University of Nebraska Medical Center, Omaha, Nebraska Medical Physiology - Radiation Technology Students, Endocrinology and Reproductive Physiology, University of Nebraska Medical Center Cardiovascular Journal Club, Department of Internal Medicine, The University of Iowa, Iowa City, Iowa Free Radical Journal Club, Department of Free Radical Biology, The University of Iowa, Iowa City, IA VBI 8020: Frontiers in Vascular Biology (2 contact hours), The Medical College of Georgia, Augusta, GA VBI 9020: Vascular Biology Journal Club (28 contact hrs), Course Director, The Medical College of Georgia, Augusta, GA VBI 9020: Vascular Biology Journal Club (1 Credit Hr/Semester), Course Director, The Medical College of Georgia, Augusta, GA Page 12

13 PHARM 701: Pharmacology Seminar (28 contact hrs), Course Director, The University of Mississippi Medical Center, Jackson, MS PHARM 702: Pharmacology Journal Club (28 contact hrs), Course Director, The University of Mississippi Medical Center, Jackson, MS ID 727: Current Issues in Biomedical Research (2 contact hrs), The University of Mississippi Medical Center, Jackson, MS PHARM 620: Introduction to Pharmacology and Therapeutics (6 contact hrs) PHARM 626: Dental Pharmacology (3 contact hrs), The University of Mississippi Medical Center, Jackson, MS PHARM 723: Mechanisms of Drug Action (4 contact hrs), The University of Mississippi Medical Center, Jackson, MS PHARM 724: Experimental Design & Methods to Study Cell Signaling (3 contact hrs), The University of Mississippi Medical Center, Jackson, MS MD-PhD Journal Club (12 contact hrs), The University of Mississippi Medical Center, Jackson, MS DEPARTMENTAL SERVICE: Medical College of Georgia, Vascular Biology Center Post-Doctoral Fellow Review Committee Member Director, Seminar Program, Department of Pharmacology, The University of Mississippi Medical Center, Jackson, MS Director, Journal Club, Department of Pharmacology, The University of Mississippi Medical Center, Jackson, MS Page 13

14 Associate Director, Pharmacology Graduate Program, Department of Pharmacology, The University of Mississippi Medical Center, Jackson, MS 2016 Member, Departmental Review Committee, Department of Pharmacology, The University of Mississippi Medical Center, Jackson, MS Member, Graduate Program Committee, Department of Pharmacology, The University of Mississippi Medical Center, Jackson, MS Member, Faculty Recruitment Committee, Department of Pharmacology, The University of Mississippi Medical Center, Jackson, MS Member, Faculty Strategic Planning Committee, Department of Pharmacology, The University of Mississippi Medical Center, Jackson, MS Member, Professional Education Committee, Department of Pharmacology, The University of Mississippi Medical Center, Jackson, MS INSTITUTIONAL SERVICE: 2009 Member, Medical Library Director Search Committee, The Medical College of Georgia Chair, MD/PhD Program Advisory Board, The University of Mississippi Medical Center, Jackson, MS Chair, MD/PhD Interview Committee, The University of Mississippi Medical Center, Jackson, MS Member, Graduate Council Committee, The University of Mississippi Medical Center, Jackson, MS Member, Medical Student Research Program (MSRP) Advisory Board, The Page 14

15 University of Mississippi Medical Center, Jackson, MS Member, Office of Research, ISRP Grant Review Committee, The University of Mississippi Medical Center, Jackson, MS Member, Obesity COBRE, Pilot Project Peer Review Committee, The University of Mississippi Medical Center, Jackson, MS 2015 Member, PIN COBRE, Pilot Project Peer Review Committee, The University of Mississippi Medical Center, Jackson, MS Member, Clinical Investigator Program (CIP) Advisory Committee, The University of Mississippi Medical Center, Jackson, MS Member, Medical Neuroanatomy Curriculum Committee, The University of Mississippi Medical Center, Jackson, MS STUDENT MENTORING: High School Students: Ria Goel, SURE Program, The University of Mississippi Medical Center Undergraduate Students: Pamela Fegan, The University of Iowa Nate Newman, The University of Iowa Anthony Taylor, The University of Iowa Tony Serfling, The University of Iowa Ashley Haskins, The University of Iowa Laura Schrader, The University of Iowa Page 15

16 Alexia Miller, Jackson State University Juan Rodriquez, Mississippi State University Leslie Miller, Jackson State University Rotating Graduate Students: Kathryn Brown, The University of Iowa Andrew Johnson, The University of Iowa Jason Davis, Medical College of Georgia Wanwisa Promsote, Medical College of Georgia Inger Stallmann-Jorgensen, Medical College of Georgia Jessica Anderson, Medical College of Georgia Yusi Wang, Medical College of Georgia Caitlin Madigan, Medical College of Georgia Jessica Faulkner, The University of Mississippi Medical Center Ellen Gllis, The University of Mississippi Medical Center Rohini Bandaru, The University of Mississippi Medical Center Kenji Maeda, The University of Mississippi Medical Center Graduate Student Advisee: Jessica Gomolak PhD, The University of Mississippi Medical Center Post-doctoral Fellow: M. Irfan Ali, MD, PhD, Medical College of Georgia Page 16

17 Medical Student Researchers: Chelsea Pyle, Medical College of Georgia Vijay Palvia, Medical College of Georgia Imad Mohamed Alabdul Razzak, Alfaisal University Saudi Arabia Ian Coate, The University of Mississippi Medical Center Medical Student Pre-clinical Advisees: Edward Yang, The University of Mississippi Medical Center Jake Wilson, The University of Mississippi Medical Center Hunter Hood, The University of Mississippi Medical Center Joey Golden, The University of Mississippi Medical Center Edmond Wright, The University of Mississippi Medical Center Tyler Howell, The University of Mississippi Medical Center Dustin Bratton, The University of Mississippi Medical Center Robert Browning, The University of Mississippi Medical Center Graduate Student PhD Thesis Committee Member: Shawn Elms, Medical College of Georgia Ashlee Tipton, Medical College of Georgia Jutamas Suwanpradid, Medical College of Georgia Maha Coucha, Medical College of Georgia Jennifer Iddings, Medical College of Georgia Depesh Pandy, Medical College of Georgia Xiaxiang Wang, The University of Mississippi Medical Center Page 17

18 Jessica Gomolak, The University of Mississippi Medical Center Bernadette DeRussy, The University of Mississippi Medical Center Peter Mittwede, The University of Mississippi Medical Center Xiao Zhang, The University of Mississippi Medical Center BIBLIOGRAPHY: Original Papers (Peer-reviewed) 1) Mayhan WG, Didion SP. Acute effects of ethanol on responses of cerebral arterioles. Stroke. 26: , ) Mayhan WG, Didion SP. Activation of protein kinase C does not participate in disruption of the blood-brain barrier to albumin during acute hypertension. Brain Res. 696: , ) Mayhan WG, Didion SP, Patel KP. L-arginine does not restore dilation of the basilar artery during diabetes mellitus. J Cereb Blood Flow Metabol. 16: , ) Mayhan WG, Didion SP. Effect of chronic alcohol consumption on responses of cerebral arterioles. Alcohol Clin Exper Res. 20: , ) Mayhan WG, Didion SP. Glutamate-induced disruption of the blood-brain barrier: role of nitric oxide. Stroke. 27: , ) Didion SP, Mayhan WG. Effect of chronic myocardial infarction on in vivo reactivity of skeletal muscle arterioles. Am J Physiol. 272 (Heart Circ Physiol. 41):H2403-H2408, Page 18

19 7) Didion SP, Carmines PK, Ikenaga H, Mayhan WG. Enhanced constrictor responses of skeletal muscle arterioles during chronic myocardial infarction. Am J Physiol. 273(Heart Circ Physiol. 42):H1502-H1508, ) Ikenaga H, Ishii N, Didion SP, Cornish KG, Patel KP, Mayhan WG, Carmines PK. Suppressed impact of nitric oxide on renal arteriolar function in rats with chronic heart failure. Am J Physiol. 276(Renal Physiol. 45):F79-F87, ) Didion SP, Sigmund CD, Faraci FM. Impaired endothelial function in transgenic mice expressing both human renin and human angiotensinogen. Stroke. 31: , ) Didion SP, Heistad DD, Faraci FM. Mechanisms that produce nitric oxidemediated relaxation of cerebral arteries during atherosclerosis. Stroke. 32: , ) Didion SP, Hathaway CA, Faraci FM. Superoxide levels and function of cerebral blood vessels following inhibition of CuZnSOD. Am J Physiol Heart Circ Physiol. 281:H1697-H1703, ) Ryan MJ, Didion SP, Davis DR, Faraci FM, Sigmund CD. Variability of endothelial function and blood pressure in six inbred mouse strains. Arterioscler Thromb Vasc Biol. 22:42-48, ) Didion SP, Faraci FM. Effects of NADH and NADPH on superoxide levels and cerebral vascular tone. Am J Physiol Heart Circ Physiol. 282:H688-H695, ) Didion SP, Ryan MJ, Baumbach GL, Sigmund CD, Faraci FM. Superoxide contributes to vascular dysfunction in mice that express human renin and angiotensinogen. Am J Physiol Heart Circ Physiol. 283:H1569-H1576, Page 19

20 15) Didion SP, Ryan MJ, Didion LA, Fegan PE, Sigmund CD, Faraci FM. Increased superoxide and vascular dysfunction in CuZnSOD-deficient mice. Circulation Research. 91: , ) Didion SP, Faraci FM. Angiotensin II produces superoxide-mediated impairment of endothelial function in cerebral arterioles. Stroke. 34: , ) Ryan MJ, Didion SP, Mathur S, Faraci FM, Sigmund CD. PPARg agonist rosiglitazone improves vascular function and lowers blood pressure in hypertensive transgenic mice. Hypertension. 43: , ) Ryan MJ, Didion SP, Mathur S, Faraci FM, Sigmund CD. Angiotensin II induced vascular dysfunction is mediated by the AT1 A receptor in mice. Hypertension. 43: , ) Didion SP, Kinzenbaw DA, Fegan PE, Didion LA, Faraci FM. Overexpression of CuZn-SOD prevents lipopolysaccharide-induced endothelial dysfunction. Stroke. 35: , ) Faraci FM, Didion SP. Vascular protection: Superoxide dismutase isoforms in the vessel wall. Arterioscler Thromb Vasc Biol. 24: , ) Didion SP, Faraci FM. Ceramide-induced impairment of endothelial function is prevented by CuZnSOD overexpression. Arterioscler Thromb Vasc Biol. 25:90-95, ) Didion SP, Lynch CM, Baumbach GL, Faraci FM. Impaired endotheliumdependent responses and enhanced influence of rho-kinase in cerebral arterioles in type II diabetes. Stroke. 36: , Page 20

21 23) Didion SP, Kinzenbaw DA, Faraci FM. Critical role for CuZn-superoxide dismutase in preventing angiotensin-ii-induced endothelial dysfunction. Hypertension. 46: , ) Faraci FM, Modrick ML, Ryan MJ, Lamping KG, Didion SP. Cerebral vascular effects of angiotensin II: new insights from genetic models. J Cerebral Blood Flow Metabol. 26: , ) Faraci FM, Modrick ML, Didion LA, Fegan PE, Didion SP. Selective cerebral vascular dysfunction in Mn-SOD deficient mice. J Appl Physiol. 100: , ) Baumbach GL, Didion SP, Faraci FM. Structure of cerebral arterioles in mice deficient in expression of the gene for CuZn superoxide dismutase. Stroke. 37: , ) Didion SP, Kinzenbaw DA, Schrader LI, Faraci FM. Heterozygous CuZnSOD- deficiency reveals a vascular phenotype with aging. Hypertension. 48: , ) Didion SP, Lynch CM, Faraci FM. Cerebral vascular dysfunction in TallyHo mice: a new model of type II diabetes. Am J Physiol Heart Circ Physiol. 292:H1579- H1583, ) Brown K, Didion SP, Andresen JJ, Faraci FM. Effect of aging, MnSOD deficiency, and genetic background on endothelial function: evidence for MnSOD haplosufficiency. Arterioscler Thromb Vasc Biol. 27: , ) Schrader LI, Kinzenbaw DA, Faraci FM, Didion SP. Interleukin-6 deficiency protects against angiotensin II-induced endothelial dysfunction and hypertrophy. Arterioscler Thromb Vasc Biol. 27: , ) Chrissobolis S, Didion SP, Kinzenbaw DA, Schrader LI, Dayal S, Lentz S, Faraci Page 21

22 FM. Glutathione peroxidase-1 plays a major role in protecting against angiotensin IIinduced vascular dysfunction. Hypertension. 51: , ) Didion SP. Chlamydophila pnemonia and endothelial activation: The smoke that precedes the fire of atherosclerosis? Circulation Research. 102: , ) Modrick ML, Didion SP, Lynch CM, Dayal S, Lentz SR, Faraci FM. Role of hydrogen peroxide and the impact of glutathione peroxidase-1 in regulation of cerebral vascular tone. J Cereb Blood Flow Metab. 29(6):1130-7, ) Modrick ML, Didion SP, Sigmund CD, Faraci FM. Role of oxidative stress and AT1 receptors in cerebral vascular dysfunction with aging. Am J Physiol Heart Circ Physiol. 296(6):H1914-9, ) Didion SP, Kinzenbaw DA, Schrader LI, Chu Y, Faraci FM. Endogenous interleukin-10 inhibits angiotensin II-induced vascular dysfunction. Hypertension. 54:619-24, ) Didion SP. Tacrolimus-induced hypertension: what s endothelial and hematopoietic FKBP12 got to do with it? Hypertension. 57: , ) Didion SP. Antioxidant 1 in hypertension: more than just a copper chaperone. Hypertension. 60: , ) Li W, Prakash R, Chawla D, Du W, Didion SP, Filosa JA, Zhang Q, Brann DW, Lima VV, Tostes RC, Ergul A. Early effects of high fat diet on neurovascular function and focal ischemic brain injury. Am J Physiol. Regul Integr Comp Physiol. 304:R1001-R1008, ) Kinzenbaw DA, Chu Y, Pena Silva RA, Didion, SP, Faraci FM. Interleukin-10 protects against vascular dysfunction with aging. Physiol Rep. 1:e00149, Page 22

23 40) Fan F, Sun CW, Maier KG, Williams J, Didion SP, Falck JR, Zhuo J, Roman RJ. 20-hydroxyeicosatetraenoic acid contributes to the inhibition of K+ channel activity and vasoconstrictor response to angiotensin II in renal arterioles. PloS One. 8:e82482, ) Lynch CM, Kinzenbaw D, Chen X, Zhan S, Mezzetti EM, Filosa J, Ergul A, Faraci FM, Didion SP. Nox2-derived superoxide contributes to cerebral vascular dysfunction in diet-induced obesity. Stroke. 44: , ) Gomolak JR, Didion SP. Role of innate immunity in hypertension. Med Hypotheses. 83: , ) Gomolak JR, Didion SP. Angiotensin II-induced endothelial dysfunction is temporally linked with increases in interleukin-6 and vascular macrophage accumulation. Front Physiol. 5:396, doi: /fphys , ) Ali MI, Chen X, Mezzetti EM, Didion SP. Heterozygous endothelial nitric oxide synthase is associated with endothelial dysfunction in diet-induced obesity. Physiol Reports. 3:e12630, ) Didion SP. New insights into mechanisms associated with angiotensin II-induced vascular hypertrophy and remodeling. Hypertension. 67: , ) McPherson KC, Taylor L, Johnson AC, Didion SP, Geurts AM, Garrett MR, Williams JM. Early development of podocyte injury independent of hyperglycemia and elevations in arterial pressure in non-diabetic obese Dahl SS leptin receptor mutant rats. Am J Physiol Renal Physiol. 311:F793-F804, ) Altara R, Didion SP, Booz GW. Conflicting mechanisms of AT2 receptor cardioprotection revealed. Cardiovasc. Res. 112: , ) Altara R, Harmancey R, Didion SP, Booz GW, Zouein FA. Cardiac STAT3 Page 23

24 deficiency impairs contractility and metabolic homeostasis. Front Pharmacol. 7:436, ) Ge Y, Fan F, Didion SP, Roman RJ. Impaired myogenic response of the afferent arteriole contributes to the increased susceptibility to renal disease in Milan normotensive rats. Physiol Rep. 5:e13089, ) Didion SP. Heterozygous enos deficient mice as a model to examine the effects of enos haploinsufficiency on the cerebral circulation. J Neurol Neuromed. 2:6-9, ) Didion SP. A novel genetic model to explore the Brenner hypothesis: Linking nephron endowment and number with hypertension. Submitted. 52) Didion SP. Heterozygous enos deficiency is associated with endothelial function with age and is sex dependent. Submitted. 53) Wang X, Garrett MR, Didion SP. Direct correlation between blood pressure and nephron endowment in a genetic mouse model of hypertension. In Preparation. 54) Didion SP. Angiotensin II contributes to both hypertension and endothelial dysfunction in BPH2 hypertensive mice. In Preparation. 55) Didion SP. Lipopolysaccharide signaling promotes oxidative stress and endothelial dysfunction in the vascular wall. In Preparation. Abstracts (*=Peer-reviewed) 1) Didion SP, Morrow RJ, Stratton DB. Contractility changes induced by endothelial disruption: Mediation by receptor-operated calcium channels. FASEB J. 8:A612, Page 24

25 2) Didion SP, Zucker IH, Mayhan WG. Effect of chronic myocardial infarction on in vivo reactivity of skeletal muscle arterioles. FASEB J. 10:A56, ) Mayhan WG, Didion SP. Effect of glutamate on the cerebral microcirculation. FASEB J. 10:A31, ) Didion SP, Mayhan WG. Enhanced vasoconstrictor responses of skeletal muscle arterioles during heart failure: role of impaired synthesis/release of nitric oxide. Microcirculation. 4:170, ) Didion SP, Mayhan WG. Impaired endothelium-dependent response of skeletal muscle arterioles during chronic heart failure: role of oxygen radicals. FASEB J. 11:A44, ) Didion SP, Ishii N, Mayhan WG, Carmines PK. Attenuated nitric oxide synthase and superoxide dismutase activities during experimental heart failure. FASEB J. 12:A380, ) Didion SP, Sigmund CD, Faraci FM. Responses of thoracic aorta in hypertensive mice expressing the human renin and human angiotensinogen genes. FASEB J. 13:A512, ) Didion SP, Faraci FM. Evidence for an NADH/NADPH oxidase in cerebral blood vessels. FASEB J. 14:A151, ) Didion SP, Heistad DD, Faraci FM. Role of soluble guanylate cyclase and effects of atherosclerosis on responses of cerebral arteries to nitric oxide. FASEB J. 14:A427, ) Didion SP, Weintraub NL, Faraci FM. Mechanisms of arachidonic acidinduced relaxation in murine blood vessels. FASEB J. 14:A574, ) Ryan MJ, Didion SP, Faraci FM, Sigmund CD. Variability of endothelial function and blood pressure in six inbred mouse strains. FASEB J. 15:A480, Page 25

26 12) Didion SP, Faraci FM. Role of endogenous CuZn-superoxide dismutase (SOD) in the cerebral circulation. FASEB J. 15:A126, ) Didion SP, Faraci FM. Effects of NADH on cerebral blood vessels. FASEB J. 15:A127, ) Ryan MJ, Didion SP, Faraci FM, Sigmund CD. Severely impaired aortic response to acetylcholine in 129/J inbred mouse strain. Hypertension. 33:134, * 15) Ryan MJ, Didion SP, Faraci FM, Sigmund CD. Heritability of impaired aortic endothelial function in 129P3/J mice. FASEB J. 16:A880, ) Didion SP, Faraci FM. Overexpression of CuZnSOD protects against ceramide-induced endothelial dysfunction. FASEB J. 16:A1136, ) Didion SP, Ryan MJ, Sigmund CD, Faraci FM. Increased superoxide and vascular dysfunction in CuZnSOD-deficient mice. Hypertension. 40:392, * 18) Didion SP, Ryan MJ, Faraci FM, Sigmund CD. Activation of PPARg reduces blood pressure and improves vascular function in hypertensive transgenic mice. J Hypertension. 20(Suppl 4):S14, ) Ryan MJ, Didion SP, Faraci FM, Sigmund CD. Rosiglitazone decreases blood pressure and improves vascular function in transgenic mice containing human renin and human angiotensinogen. Hypertension. 40:431, * 20) Didion SP, Didion LA, Fegan PE, Faraci FM Impaired endothelium-dependent responses in cerebral arterioles of CuZnSOD-deficient mice. Stroke. 34:251, * 21) Didion SP and Faraci FM. Angiotensin II produces superoxide-mediated impairment of endothelial function in cerebral arterioles. Stroke. 34:272, 2003.* 22) Baumbach GL, Didion SP, Faraci FM. Deficiency of copper-zinc superoxide dismutase promotes cerebral vascular hypertrophy. FASEB J. 17:A63, Page 26

27 23) Didion SP, Ryan MJ, Sigmund CD, Faraci FM. Enhanced blood pressure response to angiotensin II in CuZnSOD-deficient mice. FASEB J. 17:A857, ) Didion SP, Ryan MJ, Sigmund CD, Faraci FM. PPARg activation improves endothelial function in type II diabetes. FASEB J. 17:A857, ) Faraci FM, Lamping KG, Modrick ML, Ryan MJ, Didion SP. Cerebral vascular effects of angiotensin II: role of gender, rho kinase and AT1a receptors. FASEB J. 18:A1007, ) Didion SP and Faraci FM. Vascular dysfunction in TallyHo mice: a new genetic model of type II diabetes. FASEB J. 18:A276, ) Faraci FM, Kinzenbaw D, Didion SP. Overexpression of CuZn-SOD prevents lipopolysaccharide-induced endothelial dysfunction. FASEB J. 18:A1319, ) Didion SP, Lynch CM, Faraci FM. Impaired endothelium-dependent responses and enhanced function of rho-kinase in cerebral arterioles in type II diabetic mice. FASEB J. 18:A1007, ) Didion SP, Kinzenbaw DA, Faraci FM. Genetically-altered mice reveal a critical role for CuZnSOD in preventing angiotensin II-induced endothelial dysfunction. Hypertension. 44:508, * 30) Didion SP, Modrick ML, Sigmund CD, Faraci FM. Superoxide-mediated dysfunction of cerebral arteries in transgenic mice expressing human renin and human angiotensinogen. FASEB J. 19:A1253, ) Brown KA, Didion SP, Andresen JJ, Faraci FM. Mn-SOD deficient mice exhibit increased oxidative stress and vascular dysfunction with aging. FASEB J. 19:A201, ) Faraci FM, Modrick ML, Lynch C, Dayal S, Lentz SR, Didion SP. Role of glutathione peroxidase and hydrogen peroxide in the cerebral circulation: Insight from GPx-1 transgenic mice. Stroke. 37:686, * Page 27

28 33) Didion SP, Lynch CM, Faraci FM. Endothelial dysfunction in cerebral arterioles in a model of diet-induced obesity and type II diabetes: Role of superoxide and NAD(P)H oxidase. FASEB J. 20:A295, ) Didion SP, Kinzenbaw DA, Schrader LI, Faraci FM. Heterozygous Cu,Znsuperoxide dismutase deficiency reveals a vascular phenotype with aging. FASEB J. 20:A1452-A1453, ) Brown KA, Didion SP, Andresen JJ, Faraci FM. Effect of aging, MnSOD deficiency and genetic background on endothelial function: evidence for haploinsufficiency. FASEB J ) Chrissobolis S, Didion SP, Faraci FM. Angiotensin (Ang II)-induced oxidative stress and endothelial dysfunction in the cerebral circulation. FASEB J 20:LB15, ) Didion SP, Kinzenbaw DA, Faraci FM. Essential role of interleukin-6 in angiotensin-ii-induced endothelial dysfunction. Vascular Pharmacology. 45:e6, ) Didion SP. Heterozygous enos-deficiency produces oxidative stress and endothelial dysfunction in a model of diet-induced obesity and type II diabetes. Hypertension. 48:e35, * 39) Chrissobolis S, Didion SP, Faraci FM. Protective role of manganese superoxide dismutase against angiotensin II-induced, Nox2-dependent, cerebral endothelial dysfunction. FASEB J. 21:A1262, ) Faraci FM, Modrick ML, Lynch C, Dayal S, Lentz SR, Didion SP. Genetic evidence that cerebrovascular responses to arachidonic acid are mediated by hydrogen peroxide produced by SOD-1. FASEB J. 21:A1384; ) Didion SP, Schrader LI, Kinzenbaw DA, Faraci FM. Interleukin-6-deficiency protects against both acute and chronic angiotensin II-induced endothelial dysfunction. FASEB J. 21:A590,2007. Page 28

29 42) Schrader LI, Kinzenbaw DA, Faraci FM, Didion SP. MnSOD haploinsufficiency promotes oxidative stress and endothelial dysfunction in response to a high-fat diet. Hypertension. 50:e92,2007. * 43) Chrissobolis S, Didion SP, Kinzenbaw DA, Schrader LI, Dayal S, Lentz SR, Faraci FM. Glutathione peroxidase-1 protects against angiotensin II-induced endothelial dysfunction. Hypertension. 50:e103,2007. * 44) Modrick ML, Didion SP, Sigmund CD, Faraci FM. Role of oxidative stress and angiotensin II in cerebral vascular dysfunction with aging. FASEB J : ) Beyer AM, Guo DF, Didion SP, Sheffield VC, Rahmouni K. Bardet-diedel syndrome genes are involved in the control of vascular function. Hypertension. 53:e37,2008. * 46) Didion SP, Kinzenbaw DA, Faraci FM. Interleukin-10 protects against vascular dysfunction with aging. FASEB J. 23:805.15, ) Didion SP. Heterozygous endothelial nitric oxide synthase deficiency reveals a vascular phenotype with aging that is influenced by gender. Hypertension. 54:e42,2009. * 48) Didion SP. Heterogeneity in constrictor responses among selected inbred mouse strains: influence of rho-kinase. FASEB J. 24:986.13; ) Didion SP, Chen X, Mezzetti EM, Ali MI. Interleukin-6 contributes to endothelial dysfunction in the cerebral circulation in aging. FASEB J. 25: , ) Didion SP, Sarmiento JA, Mezzetti EM. Mammalian target of rapamycin (mtor) inhibition attenuates angiotensin II-induced hypertension and endothelial dysfunction. Hypertension. 60:A427;2012 * Page 29

30 51) Palvia V, Pyle C, Mezzetti EM, Chen X, Didion SP. Toll-like receptor 4 contributes to hypertension and endothelial dysfunction produced by angiotensin II. FASEB J. 25: , ) Gomolak JR, Faulkner J, Didion SP. Temporal effects of chronic angiotensin II infusion on blood pressure and vascular function. FASEB J. 27:654.22, ) Faulkner J, Gomolak JR, Garrett MR, Didion SP. Differential gene expression profiles produced by a high fat diet in white and brown adipose. FASEB J. 27:738.3, ) Gomolak JR, Faulkner J, Didion SP. Myloid differentiation factor 88 (MYD88) does not contribute to hypertension or endothelial dysfunction produced by angiotensin II. FASEB J. 27: , ) Faulkner J, Gomolak JR, Didion SP. Interleukin-10 deficiency limits the development of obesity and insulin resistance produced by a high fat diet. FASEB J. 27:1183.6, ) Didion SP. Nox2 deficiency limits the development of insulin resistance produced by a high-fat diet. FASEB J. 27: , ) Faulkner JF, Hall JE, Garrett MR, Didion SP. Upregulation of GPR50 in a white adipose depot of diet-induced obese mice requires intact leptin signaling. FASEB J. 28:711.11, ) Didion SP, Mezzetti EM, Garrett MR. Effect of a high salt diet on endothelial function and blood pressure in a genetic model of hypertension. J Hypertens. 59) Didion SP. Unholy fire: Discovering Michael Servetus contribution to pulmonary physiology through modern historical fiction. FASEB J. 29:1028.1, ) Didion SP. Role of increased Serine1176 phosphorylation in maintaining normal endothelial responses in male and female heterozygous endothelial nitric oxide synthase (NOS)-deficient mice. FASEB J. 29:1047.6, Page 30

31 61) Didion SP, Sims JD, Baldwin ZK. Myeloid differentiation factor-88 deficiency protects against lipopolysaccharide-induced endothelial dysfunction. FASEB J. 29:625.3, ) Didion SP. Interleukin-6 produces oxidative stress and endothelial dysfunction in the absence of a single endothelial nitric oxide synthase gene. FASEB J. 29:971.10, ) Didion SP. Deficiency of vascular TLR4 protects against lipopolysaccharideinduced endothelial dysfunction and NADPH-oxidase-derived superoxide. FASEB J. 30: , ) Didion SP. Nitric oxide and soluble guanylyl cyclase mediate endothelial responses in aged female endothelial nitric oxide synthase (enos)-deficient mice. FASEB J. 30:738.1, ) Wang X, Garrett MR, Didion SP. Direct correlation between blood pressure and nephron endowment in a genetic mouse model of hypertension. Hypertension. 68:A052, Book Chapters: Didion SP, Chrissobolis S, Faraci FM. Oxidative stress in hypertension. In Oxidative Stress in Aging. Edited by Miwa S, Beckman KB, and Muller FL. Springer Theses: Didion SP. The role of the endothelium on calcium mobilization in rat aortic smooth muscle in response to phenylephrine, serotonin, and endothelin-1. In partial fulfillment for the MA degree. Didion SP. The effect of chronic heart failure on responses of skeletal muscle arterioles. In partial fulfillment for the PhD degree. Page 31

32 FELLOWSHIP AND FEDERAL GRANT SUPPORT Completed: 1994 Pre-doctoral Fellowship, Heart Failure and the Microcirculation, American Heart Association, Heartland Affiliate (940491S), Salary Support Only Pre-doctoral Fellowship, Endothelial Dysfunction of the Microcirculation During Heart Failure, American Heart Association, Heartland Affiliate (954027S), Salary Support Only Pre-doctoral Fellowship, Neuropeptides and the Microcirculation during Heart Failure, American Heart Association, Heartland Affiliate ( S), Salary Support Only Principal Investigator, Vascular Responses in Transgenic Hypertensive Mice, National Institutes of Health, Individual National Research Service Award (HL-10237), Salary Support Only Principal Investigator, University of Iowa Diabetes & Endocrinology Center Pilot and Feasibility Grant, National Institutes of Health (DK ), $20, Principal Investigator, Role of PPARg in Vascular Function During Diabetes Mellitus, American Heart Association, National Scientist Development Award ( N), $260, Principal Investigator, Cerebral Vascular Dysfunction in Response to a High-Fat Diet, American Heart Association, Heartland Affiliate Grant-In- Aid ( Z). $143,000. Page 32

33 Co-Investigator, Project 1: Vascular Protection During Hypertension: Inflammatory Mechanisms. NIH/NHLBI Program Project Grant (HL ): Oxidative Mechanisms in Vascular Disease ; Principal Investigator, Donald D. Heistad Principal Investigator, American Heart Association Southeast Affiliate, Grant-In-Aid. Mechanisms of Endothelial Dysfunction in Obesity. *Awarded but returned due to scientific overlap with R01-HL Principal Investigator, Mechanisms of Vascular Dysfunction in Diet- Induced Obesity NIH/NHLBI (R01-HL089884). $250,000/yr direct costs. Current: NIH/NHLBI (R01-HL107632) Didion (PI) Molecular Mechanisms of Hypertension in the Microcirculation Period: 5/2011-5/2017 on NCE Role: Principal Investigator $250,000/yr direct costs. National Institutes of Health/NIBIB (R01-EB020006) Janorkar (PI) 3D Spheroid Model of Adipose Pathophysiology Period: 9/2016-8/2021 Role: Co-Investigator Direct Costs: $250,000/yr Pending: NIH/NHLBI (R01-HL138106)- Didion (PI) Cardiovascular Inflammation and Circadian Disruption Period: 05/01/17-04/30/2022 Role: Principal Investigator (25% effort) $250,000/yr direct costs Page 33

34 NIH/NHLBI (R01-HL137883)- Booz (PI) Transition from Endothelial Inflammation to Diastolic Dysfunction in HFpEF Period: 05/01/17-04/30/2022 Role: Co-Investigator (5% effort) $250,000/yr direct costs NIH/NHLBI (R01-HL137789)- Pabbidi (PI) Mechanisms Underlying Cerebrovascular Function: Role of Gender and Age Period: 05/01/17-04/30/2022 Role: Co-Investigator (5% effort) $250,000/yr direct costs NIH/NHLBI (R01-HL13726)- Garrett (PI) Genetic Targets of Hypertension End Organ Damage Period: 05/01/17-04/30/2022 Role: Co-Investigator (5% effort) $250,000/yr direct costs June 2017 Page 34