PVDOMICS: Right Heart Catheterization
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1 PVDOMICS: Right Heart Catheterization Cardiovascular Physiology Core Cleveland Clinic, Cleveland OH November 7, 2016 NHLBI Pulmonary Vascular Disease Phenomics Program Funded by the National Heart, Lung, and Blood Institute of the National Institutes of Health with support from the Pulmonary Hypertension Association
2 Introduction This training is intended to serve as a reference document for PVDOMICS study coordinators who will be collecting and transmitting right heart catheterization (RHC) data. It will also include information and training on the blood draw that takes place during the right heart catheterization procedure. 2
3 Coordinator Responsibilities All right heart catheterizations will be performed by a trained and licensed medical professional. Study coordinators are responsible for ensuring that all data is obtained and transmitting that data to the Cardiovascular Physiology Core (CPC) Study coordinators may need to supervise the collection of omics blood during the RHC and assist in transporting this blood to the lab in a timely fashion. 3
4 Order of Procedures Resting Right Heart Catheterization in Supine Position Oxygen Challenge in Supine Position Exclude patients who are CO 2 retainers Vasodilatory Challenge in Supine Position Exclude if PCWP 25 Fluid Challenge in Supine Position OR Invasive CPET in Upright Position 4
5 Data To Be Obtained Pressures Saturations Outputs RA SVC Thermodilution RV PA Direct Fick (optional) PCWP PCWP PA DPG
6 Data Obtained: Blood Samples The following blood samples will be obtained during the resting RHC per the blood protocol. Venous (via introducer, before RHC) (27.5mL) Systemic Arterial (optional, before RHC) (6.5mL) Pulmonary Capillary (Wedge) (6.5mL) Pulmonary Arterial (Mix) (6.5mL) All blood samples mustreach the lab within 1 hour of the draw time. 6
7 Data Obtained: Blood Samples 1. Participant must rest for 15 min before blood collection Side port of introducer 2. VENOUS blood collection Discard 3ml OMICS blood: 27.5ml* ml Red PAX gene (RT) 2. 10ml Lavender (ice) 3. 10ml Lavender (ice) 4. 5ml Gold (ice) OPTIONAL 2a. SYSTEMIC Arterial blood collection Discard 3ml Blood gas: 1ml OMICS blood: 6.5ml* Arterial access 1. 4ml Lavender (ice) ml Red PAX gene (RT) 3. Resting RHC hemodynamic measurements 4. Measure PCW pressure Distal port 5. Pulmonary Capillary (Wedge) blood collection Discard 3ml OMICS blood: 6.5ml* 1. 4ml Lavender (ice) ml Red PAX gene (RT) 6. Measure PA pressure Distal port 7. Pulmonary Arterial (Mix) blood collection Discard 3ml OMICS blood: 6.5ml* 1. 4ml Lavender (ice) ml Red PAX gene (RT) Order of RHC Order of OMICS Blood Collection 2 Continue with the rest of the RHC procedure and challenges * non-heparinized syringe RT = room temperature 7 heparinized syringe ice = place on wet ice
8 Blood Collection Supplies Vacutainers Four 2.5mL PAXgene (Red) Two 10mL EDTA (Lavender) Three 4mL EDTA (Lavender) One 5mL SST (Gold) Blood Transfer Device Allows you to transfer blood from the syringe to the vacutainer tubes 8
9 Vacutainer Blood Tubes 2.5mL PAXgene 4mL EDTA 5mL SST 10mL EDTA 9
10 Blood Transfer Device Needs to have a femaleluer adapter! Luer adapters and holders are also sold separately 10
11 Blood Draw: Venous Venous omics Blood Draw Participant has to be resting for 15 minutes before drawing the blood. After the introducer has been placed, but beforeany other procedures or measurements are performed, draw the venous blood sample from the SVC through the side port of the sheath. Draw back 3mL of blood using a sterile syringe and discard the sample. Then, using a second sterile syringe, draw 27.5mL of blood for omics analysis. Team member or coordinator will then fill the tubes using a sterile transfer device. 11
12 Blood Draw: Venous & Optional Arterial 1. Participant must rest for 15 min before blood collection Side port of introducer 2. VENOUS blood collection Discard 3ml OMICS blood: 27.5ml* ml Red PAX gene (RT) 2. 10ml Lavender (ice) 3. 10ml Lavender (ice) 4. 5ml Gold (ice) OPTIONAL 2a. SYSTEMIC Arterial blood collection Discard 3ml Arterial access Blood gas: 1ml OMICS blood: 6.5ml* 1.4ml Lavender (ice) 2.2.5ml Red PAX gene (RT) 3. Resting RHC hemodynamic measurements Order of RHC Order of OMICS Blood Collection 2 * non-heparinized syringe RT = room temperature heparinized syringe ice = place on wet ice 12
13 Blood Draw: Pulmonary Capillary (Wedge) Blood draw must be done after all pressure measurements have been completed. Draw back 3mL of blood using a sterile syringe and discard the sample. Then use a second sterile syringe to draw 6.5mL of blood for omics analysis. Transfer the blood from the sterile syringe to the appropriate blood collection tubes using a blood transfer device. 13
14 Blood Draw: Pulmonary Capillary (Wedge) 4. Measure PCW pressure 5. Pulmonary Capillary (Wedge) blood collection Discard 3ml Distal port OMICS blood: 6.5ml* 1. 4ml Lavender (ice) ml Red PAX gene (RT) Order of RHC Order of OMICS Blood Collection 2 * non-heparinized syringe RT = room temperature heparinized syringe ice = place on wet ice 14
15 Blood Draw: Pulmonary Artery (Mix) Blood draw must be done after all pressure measurements have been completed. Draw back 3mL of blood using a sterile syringe and discard the sample. Then use a second sterile syringe to draw 6.5mL of blood for omics analysis. Transfer the blood from the sterile syringe to the appropriate blood collection tubes using a blood transfer device. 15
16 Blood Draw: Pulmonary Artery (Mix) 6. Measure PA pressure 7. Pulmonary Arterial (Mix) blood collection Discard 3ml OMICS blood: 6.5ml* 1.4ml Lavender (ice) 2.2.5ml Red PAX gene (RT) Distal port Continue with the rest of the RHC procedure and challenges Order of RHC Order of OMICS Blood Collection 2 * non-heparinized syringe RT = room temperature heparinized syringe ice = place on wet ice 16
17 Recording Data: Pressure Tracings You should receive paper printouts of pressure tracings. Total of 24 printouts There should be 2 printouts per location (RA, RV, PWCP, PA) for each condition (resting, oxygen challenge, nitric oxide challenge and fluid challenge/icpet) All pressure tracings need to have an ECG tracing on then Digitized or computer generated mean line should be recorded with all tracings. 17
18 Condition should be noted ECG tracing Measurement location must be noted somewhere Mean Line Pressure scale and Speed
19 RHC Transmission to the CPC 1. Label and De-identify the RHC printout (if necessary) a) Verify that each waveform printout has the participant s 6 digit ID #, participant s alphacodeand the date of RHC test indicated on it somewhere b) If one of the 3 elements mentioned above is missing, hand write the missing element on the RHC printout c) Verify that no identifying information exists on the RHC printout d) Cross off or obscure any identifying information that does exist on the printout (e.g. name, date of birth, SSN, MRN, initials) 19
20 RHC Transmission to the CPC 2. Scan the de-identified RHC printout into a secure computer and save it as a PDF file. 3. Name the PDF file according to the following convention: RHC_######_ac_mmddyyyy.pdf Type of Test Alphacode File Type PVDOMICS ID# Date of Test 20
21 RHC Transmission to the CPC 4. Submit RHC to DCC. Log in to SSHwith your assigned account name and password Drag and drop the PDF file into SSHhome directory for PVDOMICS on DCC server DCC will then move the file out of the home directory for processing 5. Document the date you uploaded the RHC on Form
22 40.9 RHC Transmission to the CPC 6. Complete Form 280 (paper) and enter it into the PVDOMICS Study database. 7. Keep the paper copy of Form 280 and the original waveform printouts in the participant s study file maintained at your site. 22
23 For Questions Please Contact Jennifer Kirsop, Research Coordinator CPC core group: Phone: (216)
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