Syncrostim. The second chance for open cows. Presented for single cow treatment
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1 Syncrostim The second chance for open cows Presented for single cow treatment
2 Syncrostim Equine chorionic gonadotrophin (ecg) for single cow treatment The active compound in Syncrostim is ecg, previously known as pregnant mare serum gonadotrophin (PMSG). Whilst in mares, ecg is known to induce the formation of secondary corpora lutea to support gestation, when administered to cows it stimulates both luteinising hormone (LH) and follicle stimulating hormone (FSH) activity1. Syncrostim indications are: Induction and synchronisation of oestrus and ovulation in non-cycling cattle (cows and heifers) and in ewes and ewe-lambs To be used in combination with a progestagen
3 Uses of ecg in cattle Broadly, ecg may be used to overcome anoestrus in a number of situations. Animals in anoestrus do not express heat and present with small ovaries and an absence of follicles >10mm diameter8. Anovulatory anoestrus is a major barrier to efficient reproduction in the majority of herds, particularly those practicing seasonal calving, where the window of opportunity for cows to conceive is ~90 days8. Cows with low body condition score, less than 100 days postpartum or kept on pasture, and thus under nutritional restrictions, may be susceptible to falling into anoestrus. Overcoming suckler inhibition Prolonged postpartum acyclicity in suckled beef cows, caused by inhibition of LH pulses when a calf is at foot, reduces the calf crop and is a drain on resources to beef cattle producers. LH reserves are replenished between days postpartum, yet suckling anoestrus can persist for up to 67 days3. Methods to overcome this include bull exposure, calf removal or hormonal treatment; bull exposure is often impractical and unpredictable; whilst weaning is costly, can negatively impact calf weight and lead to short subsequent cycles9. Administration of progesterone through an intravaginal device has been shown to reduce this period of anoestrus through allowing maturation of postpartum dominant follicles and their subsequent ovulation, followed by normal cycles9. Increased pregnancy rates have been observed when ecg is added to progesterone protocols in cows under nutritional stress10. Improving reproductive performance in fixed-time artificial insemination (FTAI) protocols in beef cattle Studies in beef cattle, in which P4-based FTAI protocols were supplemented with ecg concurrent with removal of the P4 insert, provided evidence that ecg increased the percentage of cows that ovulated to the FTAI protocol, increased circulating P4 following FTAI and increased fertility to FTAI6.
4 Reducing embryonic loss The increased serum progesterone following egc treatment may also support embryonic survival rates, particularly as they have been observed at a critical time for embryonic survival (days 12 and 14 of the subsequent cycle) 5. It is suggested that the higher levels of serum progesterone are the result of changes to large luteal cells as they are responsible for 80% of CL progesterone production 5. Boosting ovulation rates In stimulating growth of the dominant follicle, ecg has been shown to increase ovulation rates, especially in cows with low body condition score (BCS) or in postpartum anoestrus 10. Whilst progesterone device treatment alone can achieve pregnancy rates of 50% where the BCS >3 (1-5 scale), addition of ecg to the protocol allows similar (>50% pregnancy rate) in cows with a BCS Improving follicle maturation The LH and FSH effects of ecg treatment can enhance follicular maturation and avoid follicle regression following removal of a progesterone device. Reducing the postpartum period Postpartum anoestrus is reported to be the most important factor determining herd economy 7. Studies have shown that 6-59% of animals remain acyclic 60 days postpartum, likely due to slow follicle growth and later ovulation 7. Where ovarian activity is detected in the early postpartum period, the fate of the first wave dominant follicle is greatly variable. Studies have shown that whilst 46% may ovulate, 23% may become cystic it is important to bear this in mind when ovulation of the first recruited dominant follicle could reduce the postpartum period by 31 days 3. A recent trial 3 investigating the effects of ecg administration on day 6 postpartum on ovarian activity demonstrated ovulation of the first wave dominant follicle in 71.4% of ecg-treated cows, whilst all untreated cows failed to ovulate. Further, the same trial reported a 100% ovulation rate by day 20 postpartum in ecg-treated cows as compared to 0% in those not receiving ecg. Figure 1. Effect of ecg administration on postpartum ovarian activity 100% ovulation rate by day 20 postpartum in ecg-treated cows 71.4% ovulation rate in ecg-treated cows follicle size 0% ovulation rate by day 20 postpartum Days postpartum
5 Give a second chance to open dairy and beef cows with the SyncroPlus protocol! SyncroPlus combines the use of PRID Delta (Progesterone Intravaginal Releasing Device) with the use of Syncrostim 500IU (Figure 2). 24 hours before removal Calving Figure 2. SyncroPlus protocol Enzaprost No signs of oestrus Fixed time AI at 56 hrs or twice at 48 and 72 hrs 7 days in PRID Delta out Syncrostim 500 IU Animals receiving ecg have been shown to have enhanced: Follicular growth rates2,3 Ovulation rates3 Pregnancy rates4,5 Serum progesterone concentrations6 Oestrus rate7 AI at observed heat
6 SYNCROSTIM 500 IU lyophilisate and solvent for solution for injection for cattle and sheep. COMPOSITION: Reconstituted solution for 1 dose of 2 ml contains: 500 IU Equine serum Gonadotrophin (ecg, formerly known as PMSG). INDICATIONS: In non cycling cattle (cows and heifers) and in ewes and ewe-lambs: Induction and synchronisation of oestrus and ovulation. To be used in combination with a progestagen. DOSAGE, ROUTE AND METHOD OF ADMINISTRATION: Intramuscular use. 500 IU of equine serum gonadotrophin (ecg) per animal in one administration corresponding to 2 ml of the reconstituted solution. Dissolve the lyophilisate with 2 ml of solvent. Mix until completely dissolved to obtain a homogenous solution. The reconstituted solution should be used immediately. The product should be administered at the time of the progestagen device withdrawal. WITHDRAWAL PERIODS: Meat and offal: zero days; milk: zero days. CONTRAINDICATIONS: Studies in laboratory animals have shown teratogenic effects after administration of ecg. The safety of the veterinary medicinal product has not been established during pregnancy and lactation. Do not use during pregnancy. ADVERSE REACTIONS: ecg is an exogenous protein for species other than equine. Therefore, antigen-antibody reactions may result. In very rare cases, repeated administrations of ecg can provoke anaphylactic shock. PRECAUTIONS: Special precautions for use in animals: In case of anaphylactic shock, symptomatic treatment (e.g. adrenaline or corticosteroids) should be administered. Special precautions to be taken by the person administering the veterinary medicinal product to animals: Care should be taken when handling the product to avoid self-injection. In case of accidental self-injection, seek medical advice, and show the package leaflet. Wash hands after handling the product. Studies in laboratory animals exhibited teratogenic. effects after the administration of ecg. Pregnant women, those intending to become pregnant, or whose pregnancy status is unknown, should not use the product. Accidental spillage on the skin should be washed off immediately with soap and water. Vm 15052/4049 Please use medicines responsibly ( For more information, please contact your Territory Manager or: Ceva Animal Health Ltd, Unit 3, Anglo Office Park, White Lion Road, Amersham, Bucks. HP7 9FB Tel: Fax: SYN2-L References 1. Murphy BD (2012). Equine chorionic gonadotropin: an enigmatic but essential tool. Anim Reprod. 9(3): Bó GA, Baruselli PS, Martínez MF (2003). Pattern and manipulation of follicular development in Bos indicus cattle. Anim Reprod Sci. Oct 15;78(3-4): Rostami B, Niasari-Naslaji A, Vojgani M, Nikjou D, Amanlou H, Gerami A (2011). Effect of ecg on early resumption of ovarian activity in postpartum dairy cows. Anim Reprod Sci.128(1-4): Păcală N, Corin N, Bencsik I, Dronca D, Cean A, Boleman A, Carabă V, Papp S (2010). Stimulation of the Reproductive Functions at Acyclic Cows by Ovsynch and PRID/ ecg. Scientific Papers: Animal Science and Biotechnologies. 43(1): Souza AH, Viechnieski S, Lima FA, Silva FF, Araújo R, Bó GA, Wiltbank MC, Baruselli PS (2009). Effects of equine chorionic gonadotropin and type of ovulatory stimulus in a timed-ai protocol on reproductive responses in dairy cows. Theriogenology. 72(1): Baruselli PS, Reis EL, Marques MO, Nasser LF, Bó GA (2004). The use of hormonal treatments to improve reproductive performance of anestrous beef cattle in tropical climates. Anim Reprod Sci. Jul;82-83: Garcia-Ispierto I, López-Helguera I, Martino A, López-Gatius F (2012). Reproductive performance of anoestrous high-producing dairy cows improved by adding equine chorionic gonadotrophin to a progesterone-based oestrous synchronizing protocol. Reprod Domest Anim. 47(5): Bryan MA, Bó G, Mapletoft RJ, Emslie FR (2013). The use of equine chorionic gonadotropin in the treatment of anestrous dairy cows in gonadotropin-releasing hormone/progesterone protocols of 6 or 7 days. J Dairy Sci. 96(1): Yavas Y, Walton JS (2000). Induction of ovulation in postpartum suckled beef cows: a review. Theriogenology. 54(1): Bó GA, Baruselli PS, Mapletoft RJ (2013). Synchronization techniques to increase the utilization of artificial insemination in beef and dairy cattle. Anim. Reprod. 10(3):
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