Mycotoxin ความท าทาย
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- Ella Lyons
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2 Mycotoxin ความท าทาย
3 Key Raw material : Mycotoxin contamination Aflatoxin Zearalenone Fumonisin T2 Toxin Soy Bean Meal Corn Baked Beans Fishmeal Broken Rice Casava Rice Bran DDGS ท มา : คณะส ตวแพทยศาสตร มหาว ทยาล ยเกษตรศาสตร ว ทยาเขตกาแพงแสน
4 Production of mycotoxins Harvest Fusarium Claviceps Mycotoxins Aspergillus Penecillium Field Time Storage 4 4
5 Mycotoxin Binder How do you make sure that the mycotoxins in your grain don t impair animal health or performance? How do you make sure that mycotoxins in your feed don t impair your profit? The countries most affected by mycotoxin contamination of feed
6 How to control Mycotoxin Yeast Cellwall Clay Activated Charcoal Synthetic Enzyme Herb Quick response ** **** **** **** ** Polar Mycotoxin *** **** **** **** Nonpolar Mycotoxin ** *** **** **** Zearalenone ** *** *** *** Fumonisin * ** ** Ochartoxin ** * ph Supportive **** * Immunity *** * Palatability *** ** Liver Function ** Combine Nutrient *** **** Easy denature *** ** 6
7 Effects of mycotoxins on animals Poultry Aflatoxin Growth reduction Reduced feed efficiency Immunosupression Anemia Reduced antibiotic effectiveness Anorexia Liver damage Reduced egg production Increased mortality Reduced skin pigmentation Lethargy Reduced ability to metabolize fat, starch, and protein Increased blood clotting time Zearalenone Increased mortality Increased susceptibility to disease Increased blood clotting time Ochratoxin Nephrotoxicity Reduced feed consumption Pale beaks Weakness and lameness Ataxia Tremors Diarrhea Death T-2 Toxin Digestive disorders Increased mortality Bloody diarrhea Reduced egg production Intestinal hemorrhages Increased susceptibility to disease Deoxynivalenol Reduced reproductive performance Increased mortality Reduced feed efficiency Diarrhea Reduced growth Reduced egg production Neural disturbances Increased susceptibility to disease Increased blood clotting time Source: CAST, Dairlyland labs
8 Effects of mycotoxins on animals Swine Aflatoxin Liver damage Hydrolyzation into M1 and is eliminated in milk ( piglets) Immunosupression Acute signs: anorexia, depression, ataxia, epitasis Chronic signs: reduced feed efficiency, reduced milk production, icterus, decreased appetite Deoxynivalenol Reduced feed consumption / feed refusal Vomiting Ergot alkaloids Reduced growth Vertigo, staggers, convulsions, temporary posterior paralysis Decreased peripheral blood supply Reduced reproductive efficiency of sows Eventual death T-2 Toxin Immunosupression Frequent defecation Vomiting Weight loss Feed refusal Source: CAST, University of Minnesota Ochratoxin Reduced feed efficiency Growth reduction Liver and kidney damage Increased mortality Zearalenone Hyperestrogenism Vulvovaginitis Vaginal or rectal prolapses in pre-pubertal gilts Enlarged uterus, swollen or twisted uterus, shrunken ovaries In boars, testes atrophy, enlarged mammary glands, decreased fertility
9 Effects of mycotoxins on animals Ruminants Aflatoxin Reduced feed efficiency Growth reduction Hydrolyzation into M1 and is eliminated in milk ( humans) Tachycardia Tachypnoea Liver damage Death T-2 Toxin Feed refusal Reduced production Gastroenteritis and intestinal hemorrhages Reduced immunological response in calves Abortions Death Deoxynivalenol Reduced feed consumption / feed refusal Vomiting Reduced milk production Increased count of somatic cells in milk Reduced reproductive efficiency Esporidesmine Eczema facial Zearalenone Hyperestrogenism Vulvovaginitis Vaginal secretion Abortions Reduced feed consumption Reduced milk production Reduced reproductive efficiency Source: NCSU, CAST
10 Aflatoxins fatty liver kidney disorders leg and bone problems pigmentation problems (carcasses, egg yolk) reduced hatchability of eggs, smaller eggs, eggshell quality affected coccidiosis vaccine failure reduced immunity, lower resistance to diseases, bacteria, viruses reduced performances (weight gain, FCR, mortality)
11 Ochratoxin increased mortality reduced growth and FCR feed refusal diarrhoea, tremors and other neural malfunctions (high doses). reduced egg production weaker eggshell eggs with blood and meat spots.
12 Trichothecenes (ex T-2) lesions at the edges of the beaks lesions in the intestine. decreased feed intake, weight loss anomalous feathering in chicks drop in egg production eggs with weaker shells
13 Maximum levels of mycotoxin recommended in poultry feeds (Dr. Jowaman Khajarern Thailand) Mycotoxin (ppb) Animal AF ZEN T2 OC FMN DON Duckling < <100 <300 <300 Starter Broiler < <300 <300 Grower Broiler < <200 <500 <300 Layer/Breeder < <100 <300 <300 AF = Aflatoxin ZEN = Zearalenone T2 = T-2 toxin OC = Ochratoxin FMN = Fumonisins DON = Deoxynivalenol
14 Immune Suppressive Aflatoxin Vomitoxin, T - 2 toxin Ochratoxin Fumonisins
15
16 How do mycotoxins act? Impact to production Health,Immunity 2.Absorp Mycotoxin at intestine and transform at liver x x x x x x 1.Mycotoxin contaminate in feed Mycotoxin destroy target organ and be deactivated by liver kidney
17 Our Learning of Mycotoxin 1. Polar Mycotoxin can absorped by Clay and Yeast Cellwall: Aflatoxin 2. Non Polar Mycotoxin,have some surface charge can absorped by clay and Yeast Cellwall but need to create environment : Zeralenone 3. Non Polar Mycotoxin,No surface charge need to change Mycotoxin s structure for more polarity and synergistic effect in nano level (Nanocomposit action): Fumonisin /DON /Ochatoxin 4. We have to absorp Mycotoxin As much as Posible before get into liver 5. Liver and Kidney be Performed for Eliminate Mycotoxin 6. Mycotoxin have > 200 type and combination effect need to manage as Broad Spectrum effect 19
18 Zetatechnology L Tonic We develop Nanocomposite by enhance the absorption performance and support Enzyme s Immobilization support Biotransformer action Totally, we are the first Nanocomposite plus Biotansformer in Mycotoxin binding action Our products are Theurapeutic Program planning for Feed Industry (Prevent +Treatment before lose) 20
19 STP (cc/g) Select Raw Material : Characterization Zeta Relative Pressure (p/p0) Surface area of Zeta L-Tonic Product BET Surface area [m 2 /g] Characteristic of Surface area Average pore Total pore volume diameter (cc/g) (Nanometre) Type of pore IUPAC Zeta L-Tonic Mesopore
20 Mode of Action of Zeta technology 1 Attraction 2 Absorption Polar mycotoxins (Aflatoxin, CPA, Ergot) Ca ++ Na O + O-CH O 3 O O O ß-ceto-lactone of Aflatoxin Non-polar mycotoxins (DON, FB1, OTA, T-2, ZEA) Adsorbent Pore system: Macro Meso Micro Submicro Liquid phase
21 Select material by Characteristic s Nano structure Charateristic % Absorption Normal Clay Modified Clay Zetatechnology Zetatechnology +Biotransformer Surface Area m2/g pore diametre nm Total pore cm3/g Aflatoxin* Zearalenone* Fumonisin* Ochartoxin** Dosage per ton (KG) Cost per ton ( THB ) * Ku Vet lab ** Central lab
22 Select & Develop Raw Material Nano structure Pore Big Pore Pore size Pore volume Zeta potential Clays HSCAS Modified Clays Nano composite
23 Select & Develop Raw Material Nano structure Pore Big Pore Clays HSCAS Modified Clays Pore size Pore volume Zetapotential Nano composite
24 Select & Develop Raw Material Nano structure Nanocomposite testing : Normal Clay Nanocompozite Modified Clay Nanocompozite +Biotransformer
25 2.Enzymatic Detoxification & Biotransformer
26 Enzymatic Detoxification
27
28 Enzymatic Detoxification Enzyme Dosage % Bio transformer Zearalenone Fumonisin B1 Ochratoxin A Mycopase 50 mg/10 ml nd. nd. nd. 100 mg/10 ml nd. nd %
29 Biotransformer & Nanocomposite Performance Zeta L-Tonic product Dosage % Absorption Zearalenone Fumonisin B1 Ochratoxin A Zeta L-Tonic 100 mg/10 ml 82.7% 55.4% 90.1%
30 Our Learning of Mycotoxin 33
31 ซ ต าเทคโนโลย : นว ตกรรมสารด ดจ บช วพ ษเช อรา
32 Zeta CARE in support Environment Herbal Technology Support Liver Function 1-3,1-6 Betaglucan Immuno-stimulation Feed Intake Structural Absorption Organic Glycine Chelate Functional Supplement Zn Mn Cu Zeta Oxidant Diatomateus Earth Natural Essential oil
33 Zeta CARE : Glycine Chelate Zn Mn Cu Zn Chelate Zn, Increased Mn, Cu absorbtion of Ca Egg shell quality Immune enhancer Pigment of egg shell Mn Chelate Improved fertility and bone structure Cu Chelate Pigment of egg shell Immune enhancer
34 Key Success of Zeta technology 2 1 Composition 1 nanocomposite 2 BioTransformer 3 Support Liver Kidney Function Mode of Action 1Fast absorb before transform in liver 2 Bio Transformer 3 Support Liver Kidney Function 3 Solve Key Mycotoxin Laboratory &Field result 30 minute action
35 KLEAN Greentech Mycotoxin Nano Composite Zeta-CARE Y Y Y Y Y Y Y Y Y Y Y Y Y YY Y YY Y YY Y YY Y YY Y YY Y Y Y YY Y YY Y YY Y YY Y Y Y Y Y Y Y Y 38
36 Why Zeta-Technology work!! Zeta Bio Technology Transformer 1-3,1-6 Betaglucan Zn, Mn, Cu Zeta Care % Absorption Zearalenone %* Fumonisin %* Ochatoxin %** Aflatoxin %* * Veterinary Sci Faculty,Kasetsart University,2014 * * Central lab,2014 Source: Central lab ศ นย ห องปฏ บ ต การกลาง,ส งหาคม 2556
37 สร ปผลการทดสอบค ดเป นเปอร เซ นต ท จ บได Zeta L tonic /Zeta TOF Zeta L Tonic Zeta CCF Aflatoxin B1 50 ppb Zearalenone 200 ppb Fumonisin B ppb Percent Absorption Product Dose kg/ton Aflatoxin B1 50 ppb Zearalenone 200 ppb Fumonisin B ppb Zeta L Tonic Zeta TOF
38 44
39 ZEARALENONE DON FUMONISIN Testing Raw Material: Find out Solutions
40 Raw Material Risk Low Risk Moderate Risk High Risk 1. % Mycotoxin Positive Less than 25 % positive % More than 75 % ) 2. Mycotoxin toxic Level Less 100 % % more than150 % 3.% CV Sampling Random less 10 % % More than 35 % 4. How long of Stock Less than 7 day 7-10 ว น More than 7-10 day (ท มา : Cargill Animal Nutrition, Romer labs )
41 AVERAGE DAILY GAIN Therapeutic Program Planning TPP Normal Loss before TPP Infection TPP Profit from TPP Treatment Profit from Treatment TIME IN DAYS Death
42 Dosage of Zeta Products Preventive dose: 0.5 kg/t Standard dosage: 1.0 kg/t In case of serious problems: Small Animal : up to 2.0 kg Breeder : up to 1.5 kg
43 Zn Mn Cu Compare Zeta8 Other Premium Composition Zeta Composite Don't show composition 1) Large pore + some Clay + Yeast 2)Total pore+ some only Modified Clay High Zeta Potential + Modified Clay + Resin 3) More surcharge Zeta Transformer Some use Synthetic Enzyme 4) Natural Papain 5) Autolysis Yeast Extract Zeta Care : Liver Function some have only item 6) 1-3,1-6 Betaglucan 7) Zeta Oxidant 8) Artichoke Egg Design 9)Glycine Chelate : Zn+ Mn +Cu No design Reduce Crake Egg Absorption with Aflatoxin T2 Toxin DON
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