Sprint. Revolutionary technology for the rapid, safe and direct determination of protein

Transcription

1 Sprint Rapid Protein Analyzer Revolutionary technology for the rapid, safe and direct determination of protein

2 Benefits Its simple to use Fast analysis of all types of food No hazardous chemicals Safer than kjeldahl with better results Automates methods approved by AOAC and AACC

3 Sprint complies with approved AOAC and AACC methods Dairy Protein in Milk, Fluid Milk, Buttermilk, Half and Half, Chocolate Drink, Non-Fat Dry Milk, Ice Cream AOAC Method AOAC Method AOAC Method Grains, Pet Foods, Animal Feeds Cereal grains, oilseeds, legumes, forages, animal products, dairy products, and formulated feeds or foods AACC Method 46-14B Meat Beef, Pork, Chicken, Turkey, Hot Dog, Pepperoni, Hard Salami, Summer Sausage, Ham

4 Protein Tagging How the CEM Sprint Rapid Protein Analyzer Works

5 First Things First Amino Acids A single link in a chain In foods there are 20 different kinds of amino acids They readily link together to form chains Peptides Chains of 2 to 50 amino acids Proteins Greater than 50 amino acids About 17% of the mass of a protein is due to nitrogen

6 Protein The order that the amino acids are linked in determines the Protein s function All proteins contain the elements Carbon Nitrogen Hydrogen Sulfur

7 The 20 Amino Acids in Foods The Basic Amino Acids Alanine Arginine Asparagine Aspartate Cystine Glutamine Glutamate Glycine Histidine Isoleucine Leucine Lysine Methionine Phenylalanine Proline Serine Threonine Tryptophan Tyrosine Valine Virtually all proteins found in foods contain these basic amino acids

8 The Three Basic Amino Acids Common Amino Acid Structure Arginine Histidine Lysine

9 The Three Basic Amino Acids These are the only amino acids with basic side chains Arginine Histidine Lysine

10 α - Casein Histidine Arginine N-Terminus Lysine A Milk Protein 201 Amino Acids 27 Basic Amino Acids 15 Arginine 4 Histidine 7 Lysine 1 N-Teminus 13.4% of Amino Acids are Basic

11 itag Molecule Acid Aromatic group which absorbs light, and appears orange

12 α - Casein Protein itag itag will bind to the basic sites on the protein at ph = 2 After binding the protein drops out of solution

13 How Sprint Measures Protein

14 After binding the remaining itag is drawn through the disposable filter

15 Operating Sprint Routine operation of Sprint is easy!

16 Contain everything needed for 50 samples 50 cups 50 Filters itag Solution Bar code reader eases preparation of Sprint for analysis 1 year shelf life Stored in a laboratory environment Sprint Paks

17 Restandardize Sprint Sprint automatically restandardizes after every 50 samples It is part of the procedure for logging in a new Sprint Pak The filter photometer records the light absorbed by the itag solution LED that emits light at approximately 480 nm Sample in contained in a cuvet 480 nm low band pass filter Detected by a light-tofrequency converter

18 Select a Method and Start the Run Use a keyboard to input sample IDs as well Sprint s bar code reader for entering sample ID s

19 Use a Balance to Weigh Sample Typically 0.5 gm to 2 gm Maximum range 0.05 gm to 20.0 gm Sample size is preprogrammed into Sprint methods Best precision is obtained when using recommended sample size

20 Main Components Wash Station Disposable Cup Disposable 0.45 µm Filter Turn Table

21 Add itag itag Dispenser Turn Table has Moved to Place Cup under itag Dispenser Detector checks for presence of cup and filter

22 Homogenize and Attach Filter Homogenizer Homogenizer Enters Cup and Blends Sample Turn Table Moves Cup to Homogenizer Detector Attaches Filter

23 Analyze Sprint aspirates itag solution through filter and measures amount of itag removed by Protein Homogenizer is Cleaned None of the sample enters the detector Only filtered itag

24 Sample Cup is Removed and Discarded All sample is in the cup Sprint can store up to 36,000 runs and download them directly to a LIMS system Protein combined with itag is now on the bottom of the cup Data can be loaded onto a USB memory stick for easy transfer to a spreadsheet

25 To See Sprint in Action

26 Total Nitrogen Techniques Kjeldahl and Dumas (combustion) methods are used to measure protein Total nitrogen content is measured in samples Crude Protein Multiply total nitrogen by 6.38 for milk Other factors are used for other materials Crude protein is not true protein content Measures nitrogen from protein as well as from non-protein nitrogen sources (NPN)

27 Crude Protein Protein content is not fully correct Proportion of non protein nitrogen (NPN) within and between dairy products varies 3-8% in Milk 25-30% in Whey This is affected by the animal s diet Grams of Protein pe er Liter Total itrogen Content in Milk Non- Protein Protein

28 Crude vs. True Protein Total Nitrogen Content in Milk Crude protein Measure nitrogen From all sources Multiplies by an accepted factor Popular because it requires minimum effort Grams of Protein per Liter Non-Protein Protein True Protein Measure nitrogen only from protein sources Not often done because requires more than twice the effort Crude Protein Changes Urea Ammonia Creatinine Creatine Uric Acid Adulterants 10 Sample 1 Sample 2 True Protein May be Constant

29 Sprint Results Sprint always measures true protein Other techniques do not measure protein Kjeldahl and Dumas both measure nitrogen Apply a correction factor to express nitrogen as protein

30 A more Accurate Protein Sprint provides A green alternative A safe technique A simple way to measure protein A rapid test A more accurate test Measurement

31 Melamine Sprint removes the incentive to use melamine

32 Why add Melamine? Milk Protein costs approximately $2.25 per pound If milk has 3.15% protein Value of the protein in the truck load of milk is $3,543 A Milk Tanker Truck carries 50,000 pounds of milk If milk has 4.41% protein Value of the protein in the truck load of milk is $4,961 40% increase!

33 Comparison of Results, Milk Fluid Milk Spiked with Melamine Melamine 0.3% Produced an apparent increase in protein of 1.26% Homogenized Milk Sprint % Protein (Crude) Pure Milk Kjeldahl % Protein (Crude) Average Milk with 0.3% Melamine added Average

34 Melamine and Sprint Sprint measures only protein Sprint gives a different answer than total nitrogen methods The incentive to use melamine is removed with Sprint Why would you add a chemical when it won t increase the value of the product?

35 Green Technology No harmful chemicals Such as sulfuric acid, sodium hydroxide, potassium sulfate, mercury oxide or copper sulfate No hazardous waste for disposal The sample and non-toxic itag solution are contained within the sample cup for easy disposal. Sprint only uses non-toxic itag solution Cups and caps are recyclable

36 Advantages Of Rapid True Protein Incentive to adulterate ingredients is removed Security of food supply is improved More samples can be tested easily Raw material pricing is more accurate Process control is improved with rapid test Less impact on the environment

37 IFT Innovation Award Winner The 2008 IFT Food Expo Innovation Awards program Honors outstanding Honors outstanding innovation in products, equipment, instrumentation, technology, and services

38 Thank You John Urh Product Manager