Studies on larval nutrition of large yellow croaker

Transcription

1 Lrvi 29 Gent, Belgium Studies on lrvl nutrition of lrge yellow croker A review Qinghui Ai, Kngsen Mi Ocen University of Chin

2 Lrge yellow croker One of most economiclly importnt mrine fish in Chin Over-fishing (Pseudoscien croce ) It hs een widely cultured since 199s It hs een widely cultured since 199s fter the success in rtificil htchery

3 Mrine cge-culture culture 7, tons nnully Nutrition & feeding ecomes one of ottleneck in Nutrition & feeding ecomes one of ottleneck in htchery & culture Our l strted to study lrvl nutrition of lrge yellow croker from 1999

4 1. A histologicl study on digestive system development On the sis of oservtions on the development of digestive orgns & histology: Pro-lrve: 5 DAH Post-lrve: 6 2 DAH Juvenile : egin t 21 DAH (Mi et l. 25, J. Fish Biol)

5 2. Activities of selected digestive enzymes during lrvl development Enzymes in pncres Enzymes in intestine

6 2. Activities of selected digestive enzymes during lrvl development Digestive system mtured etween dy 23 nd 25. Enzyme dt & mouth enlrge -ment indicting: the lrve could e fed successfully with compound diet, t lest from dy 23

7 2. Activities of selected digestive enzymes during lrvl development M et l., 25, Aquculture

8 3. Lrvl Feeding Behvior 摄食量 /(m mg. fish -1 ) Feed con nsumption 2 (1) Feeding rhythm 日龄 DAH 6: 1: 14: 18: 22: 2: 6:.25 摄食量 /(mg. fish -1 ) Feed consumption 日龄 11-12DAH 6: 1: 14: 18: 22: 2: 6:.4 Crepusculr feeding 摄食量 /(mg. fish -1 ) Fe eed consumption 日龄 4-5DAH 6: 1: 14: 18: 22: 2: 6: 时间 /h Time

9 (2) Optiml Feeding Frequency Inter - i ndi vi dul vr i t i on, CV (%) The effect of feeding frequency on the survivl of lrge yellow cr oker P1 P2 P3 P4 c P1 P2 P3 P4 SGR R, % The effects of feeding frequency on SGR of lrge yellow croker lrve c c P1 P2 P3 P4 P1: 2 mel per dy P2: 4 mel per dy P3: 8 mel per dy P4: 12 mel per dy 8 mels per dy is optiml

10 (3).Feeding Attrctnts D2 with.3% ti etine+.5% +5% 饲料中不同诱食剂对大黄鱼稚鱼特定生长率的影响 nucleotide+.2%al +.1% Arg ws the est for the feeding nd growth of lrve. 1 Amino cid mixture 2 Betine+nucleotide+Al+Arg id A 3 Extrct from clmworm 4 Chinese trditionl hers 5 Commercil ttrctnt, 7 Live prey / dy) 1 SGR( % Mrueni Nisshin Feed Co., Ltd c d c 饲料号 c

11 4. Nutrient Requirements y = -.9x x R 2 =.9173 Protein y = x x R 2 =.9335 Met SGR (% d -1 ) SGR (% d 1 ) X mx =2.58% Dietry protein (% DM) Dietry methionine level (% of diet) Higher compred with juvenile

12 4. Nutrient Requirements Optiml level of Protein hydrolyste Effects on sur vi vl Sur vi vl (%) c d Diet1 (%) Diet2 (25 25%) Diet3 (5%) 1 5 Diet4 (75%) Di et 1 Di et 2 Di et 3 Di et 4 生物饵料 11 S 1 G R 9 (%/ dy) 8 7 Ef f ect on gr owt h c Di et 1 Di et 3 生物饵料 25% is optiml

13 4. Nutrient Requirements SGR( %dy y-1) Lipid nd phospholipids SGR( %dy- 1) e c 8. 3% 12. 5% 16. 4% 2. 4% 24. 8% LP 饲料脂肪水平 c d y = x x R 2 = 磷脂添加量 (%) 存活率 (%) e 8. 3% 12.5% cd 16.4% 2.4% c 饲料脂肪水平 d 24.8% Optiml lipid: 17.5% Optiml phosphlipids:5.5% LP

14 Essentil ftty cids EPA+DHA 1.86% % Sur v i v l y = x x R = ArA,%dry weight ARA %

15 Optiml Dietry Vitmin C Vc 添加量 -SGR 折线模型 SGR %/ dy ( 89. 6, 8.82) Vc mg/ kg

16 5 Protein nd lipid sources Shrimp mel + mussel mel + fish mel (5%): good! %2 sur vi vl Sur vi vl SGR Survivl in FO nd LO ws significntly higher compred with SO nd MO. SGR in SO ws significntly lower thn other groups. 5 Fish oil Linseed oil Soyen oil Mixture (1:1:1) D1 D2 D3 D4

17 6 Utiliztion of Nitrogen Sources SGR,% Survivl,% Specil growth rte 12 Growth Survivl nd Survivl % FM FM+25%CAA FM+25%DH FM:fishmel Survivl 1% FM FM+25%CAA FM+25%DH DH: fish protein hydrolyste CAA: mixture of crystlloid mino cids 1. SGR 1%FM tretment ws significntly lower thn other groups. 2. Survivl 1%FM tretment ws the lowest.

18 7 Artificil microdiet 7-1 Size of microdiet Diet must e prepred s microprticles whose size must e dpted to the size of the lrve mouth. (M et l., 25)

19 7-1 Prticle size of ingredients Ef f ect s of pr t i cl e si ze of i ngr edi ent on gr owt h of l r ge yel l ow cr oker l r ve Diet 1: 1µm 12 c Diet 2: 71µm e d 1 Diet 3: 48µm (suitle) S 8 Diet 4: 25 µm G R 6 Diet 5: Commercil diet (%/ dy) 4 Live prey LP (Di et )

20 7-2 Binder selection for micro-diets Control: No specific inder Cr: Crgeen CMS: Croxyl methyl Cellulose N SA: Alginte N Gel: Geltin drgel: LP: Live prey ention rte of y mt t er (%) ret Leghi ng r t e of di et s Con Cr CMS SA Gel i mmer si on t i me i n shki ng th( 分钟 ) 实验结束各组大黄鱼存活率 实验结束各组大黄鱼特定生长率 (SGR) 存活率 (%) c Con Cr CMS SA Gel LP 饲料 (Di et )/ 饵料组 S G R ( %/ dy) c c Con Cr CMS SA Gel LP 饲料 (Di et )/ 饵料组 c

21 8 Additives: Yest Cell Extrcted Polyscchride (YCEP) Effect of YCEP on SGR of Lrve YCEP YCEP Effect of YCEP on High-temper t ur e Resi st nce Specific Growth Rte ( %/d) c c 系列 1 Time( min) Hl f - l et hl 系列 1 YCEP suppl ement s ( %) YCEP Suppl ement s( %) Addition of YCEP significntly ifi improved growth of lrve. Addition of YCEP significntly elongted the hlf-lethl time of lrve ginst high temperture (32 )

22 8 Additives: Proiotics--Xile 333 Sur vi vl ( %) Ef f ect of Xi l e 333 on Sur vi vl of Lr ve 4 Survivl in lrve fed the diets with.2%, 3 2 系列 % nd 1.% supplementtion were significntly higher thn tht of control group. Xi l e 333 supplement s ( %) Effect of Xile 333 on High Tempr t ur e Resi st nce of Lr ve The supplementtion of Xile333 significntly l et hl t i me ( mi n) 系列 1 elongted the hlf-lethl time of lrve ginst high temperture (32 ) Hl f - l Xi l e 333 supplement s ( %)

23 Summry 1. Pro-lrve: 5 DAH;Post-lrve: 6 2 DAH; Juvenile : fter 21 DAH 2. Digestive system mtured etween dy 23 nd Lrve could e fed successfully with formulted diets, t lest from dy Crepusculr feeding ehvior. 5. Optiml Feeding Frequency: 8 mels/dy 6. Mixture of etine,nucleotide, Al & Arg ws effective feeding ttrctnt. 7. Optiml dietry levels: protein 6%, met 2.58%, lipid 17.5%, phosphlipids 5.5%, EPA + DHA 1.85%, ARA.9%, Vc 9 mg/kg

24 Summry 8. Mixed proteins (Shrimp mel + mussel mel + fish mel (5%)), FM with CAA & fish protein hydrolyste etter. 9. Prticle size of ingredients in micro-diets: < 48µm. 1. Crgeen & lginte N re suitle inders of microdiets. 11. Yest cell extrcted polyscchride & the proiotics were le to improve growth nd stress resistnce of lrve

25 Welcome to Qingdo, Chin 14th Interntionl Symposium on Feeding nd Nutrition of Fish