G. Hajarooba, N.Y. Hirimuthugoda and K. Radampola

Transcription

1 World Journl of Fish nd Mrine Sciences 9 (3): 17-24, 2017 ISSN IDOSI Pulictions, 2017 DOI: /idosi.wjfms Effect of Proiotics on the Growth Performnce nd Survivl of Fresh Wter Prwn (Mcrorchium rosenergii) Fed on Formulted Diets Supplemented with Proiotics G. Hjroo, N.Y. Hirimuthugod nd K. Rdmpol 1 Deprtment of Animl Science, Fculty of Agriculture, University of Jffn, Ariviyl Ngr, Kilinochchi (44000), Sri Lnk 2 Deprtment of Animl Science, Fculty of Agriculture, University of Ruhun, Sri Lnk 3 Deprtment of Fisheries nd Aquculture, Fculty of Fisheries nd Mrine Sciences nd Technology, University of Ruhun, Wellmdm, Mtr (81000), Sri Lnk Astrct: Two experiments were conducted to study the effects of proiotic Biosyn-AQ (comprising Lctocillus sp, Bifidocterium nd Streptococcus) on growth nd survivl of gint freshwter prwn, Mcrorchium rosenergii fed on formulted diets contining proiotics. In experiment-1 (6 weeks), three different dietry tretments; Control diet without proiotics (Commercil feed with 42% Protein [P]) nd two formulted diets with proiotics Biosyn-AQ nd vrying levels of protein; Diet-1 (proiotics 4 g/kg of feed nd 35% P) nd Diet 2 (proiotics 4 g/kg of feed nd 33% P) were used in triplicte groups. Post lrve (PL) were stocked t rte of 40PL/tnk (60 cm 30 cm 30cm). Feeding ws done t 10% of ody weight per dy nd st prwns were weighed every 2 weeks. At the end of the 1 experiment, Growth prmeters; finl lengths nd weights, length gin ( cm), weight gin ( g) nd specific growth rte ( ) of prwns showed no significnt difference etween tretments. Significntly lower feed conversion rtios (0.96±0.16, 1.18±0.18) nd higher survivl (80.00±10.89 %, 62.50±12.5 %) were recorded for control diet nd diet-1 respectively. Feed costs for diet 1 nd 2 were lower compred to the price of control diet. However, PL fed on diet-1 performed on pr with PL fed on protein rich control diet in terms of etter growth performnce nd nd selected s sl diet for 2 experiment. In experiment-2 (4 weeks), Bsl diet (35% crude protein) ws prepred y the supplementtion of proiotics t 3 different concentrtions ie; 1%, 2% nd 3% of the diet. Prwns were stocked t rte of 20 juveniles / tnk. Significntly higher length gin, weight gin, specific growth rte nd survivl rte were recorded for prwns fed on diets supplemented with 2% nd 3% proiotics thn those fed on diet supplemented with 1% proiotics fed group. On the other hnd, diet with 2% proiotics performed sttisticlly in pr with diet with 3% proiotics. Therefore, 2% of proiotics ws selected s n idel concentrtion in terms of low cost nd etter growth performnce. From oth experiments, it cn e concluded tht diet-1 formultion supplemented with 2% proiotics Biosyn-AQ leds to etter growth performnce of prwns nd cn e suggested for promoting sustinle culture of M.rosenergii under Sri Lnkn conditions. Key words: Post Lrve Juveniles Proiotics Biosyn-AQ INTRODUCTION vlue nd omnivorous hit hve mde the species s n excellent cndidte for quculture [2]. In Sri Lnk, the The gint freshwter prwn (Mcrorchium production of M.rosenergii is sed on cpture, minly rosenergii) is n economiclly importnt qutic from reservoirs nd flood plins, such s the villus of crustcen species, undnt in the south-southest Asi Mhveli River [3] nd freshwter prwn culture gins nd Asi pcific region [1]. Its superior tste, nutritionl momentum in the inlnd fisheries sector tody. Corresponding Author: G. Hjroo, Deprtment of Animl Science, Fculty of Agriculture, University of Jffn, Ariviyl Ngr, Kilinochchi (44000), Sri Lnk. Tel: , Fx:

2 World J. Fish & Mrine Sci., 9 (3): 17-24, 2017 An pproprite feeding is mjor fctor ehind the In experiment-1, the ingredients of Diet-1 nd 2 were success in quculture. Feed costs constitute 40-60% of weighted s per formule, mixed properly; stem cooked opertionl costs in production of the freshwter prwn for 15 minutes nd cooled down to room temperture. [4]. The types of feed used in freshwter prwn frming Then the feed mixture ws mixed with proiotics t the minly include live feeds nd rtificil feeds. At higher rte of 4g freeze dried powder per kg of feed mixture, mde stocking densities prwn individuls my e more into dough n finlly mde into pellets y using loclly dependent on rtificilly prepred diets [5]. The use of mde hnd pellet mchine. The pellets were dried in proiotics in the culture of qutic orgnisms is o thermosttic oven t 40 c until it reched constnt weight incresing in demnd for more environment friendly nd stored in polythene gs t room temperture. quculture prctices [6]. Use of different commercil Commercil feed for P. monodon which is currently used proiotics y dministering them through feed of to feed M. rosenergii in grow-out ponds in Sri Lnk M. rosenergii ws reported [7]. Growth nd survivl ws used s the control diet without proiotics. Protein rtes of M. rosenergii fed on the proiotic supplements contents of the control nd experimentl diets were were significntly greter thn those of the controls determined y Kjeldhl method. In experiment-2, selected [8-11]. sl diet from experiment-1 ws incorported with 3 Development of semi-intensive quculture of different concentrtions (1%, 2% nd 3%) of proiotic M.rosenergii s commercil industry in Sri Lnk is Bio-syn AQ. limited minly due to unvilility of suitle commercil feeds. At present, the widely used commercil feed in Feeding Experiments prwn frming is poultry feed strter-i, which is of low Experiment-1: M. rosenergii Post Lrve (PL-35) with nutritive vlue. However some frmers use the commercil the verge length nd weight of 1.64±0.25 cm nd 0.08 g feed of Peneus monodon to feed the M.rosenergii post respectively were stocked t stocking density of 40 lrve [12]. Developing n rtificil feeding regime with PL/tnk in glss quri (60 cm 30 cm 30 cm). Three proiotics is highly desirle in order to sustin the different dietry tretments; Control diet without growth of fresh wter prwn frming nd exports of Sri proiotics (Commercil feed with 42% Protein [P]) nd two Lnk. However such studies re scrce in Sri Lnk. formulted diets with proiotics Bio-syn AQ nd vrying Therefore, the present study ws conducted to levels of protein; Diet-1 (proiotics 4 g/kg of feed nd evlute the growth prmeters of fresh wter prwn fed 35% P) nd Diet 2 (proiotics 4 g/kg of feed nd 33% P) on formulted diets supplemented with proiotic-biosyn were used in triplictes. The experiment ws crried out AQ in qurium conditions to select n pproprite, cost for 7 weeks. effective rtificil diet formultion comined with est proiotic inclusion level. Experiment-2: Prwn juveniles with the verge length nd weight of 3.75±0.26 cm nd 1.02±0.29 g respectively MATERIALS AND METHODS were stocked in glss quri in the stocking density of 20 juveniles per tnk. Three experimentl diets with 1%, 2% Post Lrve (PL 20) of Fresh wter prwn were stocked nd 3% inclusion levels of proiotics Bio-syn AQ were in glss tnks (60 cm 30 cm 30 cm) filled with fresh fed to juveniles for period of 4 weeks. wter nd were cclimtized to lortory conditions for In oth experiments, PLs / juveniles were fed twice 2 weeks (up to PL 35) efore the commencement of dy (8.00 m nd 5.00 pm) t rte of 10% of the ody experiments. The tnks were clened on dily sis y weight. Prwns in ech replicte were counted in fortnight siphoning the tnk ottom. Aertors were connected to intervl nd 10 rndomly selected prwns were weighed tnks to provide continuous mild ertion. Smll pieces of nd their length mesured in every 2 weeks. Uneten feed, PVC pipes were put into tnks s shelter for prwn to feces nd moult (if ny) were siphoned out every dy in minimize the cnnilism mong prwns. the morning. Diet Preprtion: The experimentl diets (Diet-1 nd Diet- Evlution of Growth Prmeters: After the feeding tril, 2) were formulted y considering the stndrd dietry the growth prmeters such s weight gin (WG) nd protein requirements of prwns (Tle 1). length gin (LG), survivl rte, specific growth rte (S.G.R) Proiotics used is Bio-syn AQ comprising nd feed conversion rtio (F.C.R) were individully Lctocillus sp, Bifidocterium nd Streptococcus. determined y the following equtions [13]. 18

3 World J. Fish & Mrine Sci., 9 (3): 17-24, 2017 Weight gin (g) = Men finl prwn weight - Men initil prwn weight Length gin (cm) = Men finl prwn length Men initil prwn length No. of totl live prwn Survivl rte (%) = 100 Totl no. of prwn stocked log W2 log W S.G.R (% per dy) = t W nd W = Initil nd Finl weight (g) respectively 1 2 t = Totl numer of experimentl dys Totl weight of feed consumed (dry) F.C.R = Biomss gin (wet) Wter Qulity Anlysis: Wter temperture nd ph were mesured once dy y using thermo meter nd ph meter (Model: AD131, U.K) respectively. Dissolved oxygen ws mesured once week y using the Field DO meter (Model: YSI Pro-20, USA) wheres turidity ws mesured weekly using portle turidity meter (HACH 2100P, USA). Sttisticl Anlysis: A one-wy nlysis of vrince (ANOVA; SPSS version: 16.0) ws used to determine whether significnt vrition etween the tretments existed. Differences etween mens were determined nd compred y the post hoc multiple comprison test (DMRT). All tests used significnce level of P <0.05. Dt re reported s men± stndrd devition. RESULTS Wter Qulity: Men vlues for wter qulity prmeters during experiment-1 nd 2 re tulted in Tle 2 nd Tle 3 respectively. During the oth experimentl periods, wter temperture in glss quri rnged from C, ph rnged from 6.86 to 7.33, turidity vried from 1.23 to 2.40 NTU nd dissolved oxygen vried from 7.58 to 7.69 mg/l. Growth Performnce of Fresh Wter Prwns: Experiment 1: Growth prmeters nd survivl of fresh wter prwn post lrve (PL) under different tretments of st 1 experiment re presented in Tle 4. After 7 weeks, there were no significnt differences (p>0.05) in finl lengths nd weights of PLs fed on control diet, diet-1 nd diet-2. It showed tht the experimentl Tle 1: Proximte composition nd cost of formulted diets Composition Ingredients Diet 1 Diet 2 Fish mel (g/100g) Soy en mel (g/100g) Coconut mel (g/100g) Rice rn (g/100g) Whet flour (g/100g) Vegetle oil (g/100g) Vitmin nd minerl premix (g/100g) Totl Cost/Kg (LKR) Crude protein (%) Crude ft (%) 8 8 Tle 2: Men ± SD for the wter qulity prmeters in the glss quri of M. rosenergii post lrve fed with two experimentl diets with proiotics nd control diet during experiment-1 Tretments Prmeters Control diet Diet-1 Diet-2 Wter temperture ( C) 28.42± ± ±0.04 Turidity (NTU) 1.40± ± ±0.27 ph 6.88± ± ±0.02 Dissolved oxygen (mg/l) 7.58± ± ±0.01,, c Ech vlue is men ± SD of three replicte, within row, mens without common superscript differ (P < 0.05) Tle 3: Men ± SD for the wter qulity prmeters in the glss quri of post-lrve of M. rosenergii fed with three experimentl diets incorported with different proiotic concentrtions (1%, 2% nd 3%) during experiment-2 Tretments: Level of proiotics Prmeters 1% 2% 3% Wter temperture ( C) 30.05± ± ±0.07 Turidity (NTU) 1.72± ± ±0.18 ph 7.33± ± ±0.03 Dissolved oxygen (mg/l) 7.63± ± ±0.02,, c Ech vlue is men ± SD of three replicte, within row, mens without common superscript differ (P < 0.05) diets 1 (35% protein) nd 2 (33% protein) with proiotics were performed in pr with control diet (42% protein) without proiotics. Significntly higher (p<0.05) survivl rtes were recorded under control diet nd diet-1 where s diet-2 showed the lowest survivl rte. Effect of control nd experimentl diets were not significnt on growth prmeters such s Percent length nd weight gin nd SGR of prwn PLs. Length nd weight gins vried from cm nd g respectively. SGR vlues found to e % (Tle 4). In cse of FCR, significntly lowest vlues were oserved for control diet nd diet-1 compred to the diet-2 fed group of Pls. 19

4 World J. Fish & Mrine Sci., 9 (3): 17-24, 2017 Fig. 1: Men ody weight vritions of M.rosenergii fed with control diet nd two experimentl diets t different smpling weeks during experiment-1 Tle 4: Men ± SD of growth prmeters nd survivl of post-lrve of M. rosenergii fed on control diet without proiotics nd two st experimentl diets with proiotics during 1 experiment Tretments Prmeters Control Diet-1 Diet-2 Initil length (cm) 1.64± ± ±0.25 Finl length (cm) 3.63± ± ± Initil weight (g) 0.08± ± ±0.00 Finl weight (g) 0.78± ± ±0.18 Survivl rte (%) 80.00± ±12.5 c 45.00±6.61 Length gin (cm) 1.99± ± ±0.50 Weight gin (g) 0.70± ± ±0.18 SGR (% per dy) 2.29± ± ±0.24 FCR (g) 0.96± ± ±0.66,, c Ech vlue is men ± SD of three replicte, within row, mens without common superscript differ (P < 0.05) Tle 5: Men ± SD of growth prmeters nd survivl of post-lrve of M. rosenergii fed on three experimentl diets with 1%, 2% nd nd 3% inclusion levels of proiotics during 2 experiment Tretment: Level of proiotics Prmeters 1% 2% 3% Initil length (cm) 3.75± ± ±0.26 Finl length (cm) 4.16± ± ± Initil weight (g) 1.02± ± ±0.31 Finl weight (g) 1.18± ± ±0.22 Survivl rte (%) 62.5± ± ±3.54 Length gin (cm) 0.41± ± ±0.14 Weight gin (g) 0.16± ± ±0.22 SGR (% per dy) 0.11± ± ±0.66,, c Ech vlue is men ± SD of three replicte, within row, mens without common superscript differ (P < 0.05) Men ody weights of PLs up to first two weeks were more or less similr nd then rpid increses in weights recorded for control diet thn experimentl rd diets during 3 week. But the experimentl diet fed groups were performed grdully nd lmost reched the th weights of control diet fed groups t the end of 6 week (Figure 1). Growth Performnce of Fresh Wter Prwns: Experiment 2: The growth dt of prwn juveniles in experiment-2 re given in Tle 5. Diet-1 ws selected from the experiment-1 nd used s sl diet formultion in experiment-2 with 3 different inclusion levels of proiotics (1%, 2% nd 3%). Men finl lengths nd weights of prwn juveniles were significntly higher for diet with 2% (4.73±0.18 cm; 1.88±0.16 g) nd 3% proiotics (4.78±0.61 cm; 1.96±0.22 g) when compred to diet with 1% proiotics (4.16±0.54 cm; 1.18±0.10 g). Prwns fed on 2% nd 3% proiotic supplemented diets showed significntly higher survivl rtes. Furthermore, men vlues recorded for growth prmeters such s length gin, weight gin nd SGR were significntly higher for 2% nd 3% proiotic supplemented diets thn 1% proiotic supplemented diet fed group juveniles. Hence, the effects of different proiotic inclusion levels were not significnt on ll growth prmeters nd survivl etween 2% nd 3% proiotic fed groups (Tle 5). DISCUSSION Wter Qulity: Growth nd survivl in prwn lrve re directly relted to mintennce of good wter qulity, food qulity nd quntity nd vilility of the prwns of cquire the food [14-16]. Temperture, turidity, ph nd dissolved oxygen recorded in the present study were found to e within the cceptle limits for M.rosenergii reported in vrious litertures [12, 17-20]. This might e ttriuted to the supplementtion of proiotics with the experimentl diets of the current study [21]. Experimentl Diets nd Proiotics: In view of the high cost of cysts nd their occsionl scrcity, too much dependence on Artemi (mjor live feed) is mjor constrint in the expnsion of M. rosenergii htcheries 20

5 World J. Fish & Mrine Sci., 9 (3): 17-24, 2017 [22]. Control (42% protein) nd the experimentl diets Tuifex, clm met nd egg custrd [37] might e due to (Diet-1: 35% protein; Diet-2: 33% protein) used in the the higher nutritive vlue, especilly the higher content of present study meet the protein requirement of fresh wter crude protein of those food items (44-66%) thn tht of prwns ccording to finding tht the M. rosenergii the experimentl feeds used in the present study. required 30-40% protein s optimum in the diet [24]. Survivl rtes of the current experiment indictes Likewise there were severl litertures [25-28] in rnge of (45-80%) in which control diet fed group hd greement with the protein content of the diets used in significntly higher survivl (80%) which my e due to this study in terms of higher growth for M.rosenergii its higher protein content leds to helthier nd roust such s dietry protein levels of 40%, 30-38%, 35% nd PLs. Survivl rtes recorded y Hossin et l. [38] 25-35%. The protein content in the commercilly ville reported tht the survivl (%) of the prwns rnged poultry feed strter-i (20-21%), which is used to feed the etween 46.6 to 66.6 % in monoculture in erthen post-lrve of M. rosenergii in semi-intensive pond ponds; Kumr et l. [39] reported tht the survivl rte of culture in Sri Lnk t present is less thn the minimum M. rosenergii monoculture ws 62.4%, Sdek & El-Gyr required level of 23% [29] wheres in the formulted feeds [40] reported tht the survivl rtes of 62-80% re more or used in the present study, these vlues were higher thn less similr to tht of present study. However, survivl the required minimum. rtes recorded in the experiment-1 were higher thn those recorded y severl litertures [41-43] which were 11.9%, Growth Performnce: Experiment-1: Optimiztion of 10.2% nd 30%. Survivl rte vlues ttined for control growth hs received high ttention mong growers nd diet nd diet-1 were higher thn those recorded when fed reserchers. Becuse the size of post-lrve t stocking with Artemi (44%) or Moin (33%) lone nd diet ply n importnt role in getting the mximum consisting of 50:50 comintions of Artemi nd Moin productivity s well s the mximum profit in the (56%) [44]. Diets 1 nd diet-2 fed groups showed lower quculture of M. rosenergii [32]. Performnce of diet-1 survivl rte compred to control diet fed group might e nd diet-2 fed group of PLs were sttisticlly in pr with due to slightly lower CP of those diets thn control diet, control diet in terms of growth performnce regrding ll incidence of cnnilism nd loss of viility of growth prmeters; finl lengths nd weights, length nd proiotics. Cnnilism is inevitle in crustcen culture. weight gins nd SGR except survivl nd FCR. Length Therefore, survivl cnnot e lwys considered to e gins (1.83 to 1.99 cm) in the present experiment-1 were directly originted from the nutritionl conditions. Long lower thn tht of length gins ( cm) wheres lrvl phse (Compred to peneid shrimps) nd the weight gins were more or less similr to the vlues cnnilistic nture of lrve of freshwter prwn hve ( g) recorded y Murlishnkr et l. [33] when resulted in poor survivl in the commercil htcheries in M.rosenergii fed with different oil supplemented feeds Indi [45]. Venkt et l. [34] pointed out tht the survivl with 40% CP. But higher vlues for weight gins ( performnce of M. rosenergii PL supplemented with g) were oserved in the present study thn tht of vlues L. cidophillus nd L. sporogenes diets with 35% CP hd ( g) recorded y Amreweer et l. [12] when 100% survivl. But the results of survivl of prwns fed prwns fed with formulted diets (33-35% CP) on proiotic Biosyn-AQ supplemented diet-1 nd diet-2 incorported with unutilized protein sources such s of the experiment- 1 hs not supported the ove finding mussel met, trsh fish nd cttle intestine during 90 dys. might e due to loss of proiotic viility nd lower Higher vlues oserved for specific growth rte dpttion of prwns to glss qurium conditions. indictes tht lrger size cn e ttined with lesser time. FCR which is the feed mount needed to increse SGRs oserved in the present experiment re supported single unit of the ody weight. Significntly lowest vlues y SGRs recorded y Amreweer et l. [12] when of FCR nd highest vlues for FCE were otined for prwns fed with formulted diets (33-35% CP) control diet nd diet-1 when compred to diet-2. FCRs incorported with unutilized protein sources nd higher recorded for control diet nd diet-1 in the present study thn those recorded in the feeding experiments with were more or less similr to the vlues recorded y Lctocillus-sed proiotics [34], diets enriched with Prkrm et l. [35] in their feeding experiments with vitmins E nd D, cod liver oil nd stxnthin [35], diets vitmins enriched formulted feeds ( ). However, supplemented with proiotics BinifitTM [36]. However, present FCR vlues for control nd diet-1 were lower the SGRs recorded in the present experiment re lower thn the vlues recorded in feeding experiments y thn those recorded when fed with Artemi, Moin, Venkt et l. [34] with Lctocillus-sed proiotics 21

6 World J. Fish & Mrine Sci., 9 (3): 17-24, 2017 ( ), Amreweer et l. [12] with unutilized protein growth prmeters. In the present experiment, results re sources ( ) nd Seenivsn et l. [10] with in greement with the ove findings. Therefore, diet with different proiotic concentrtions ( ). 2% nd 3% proiotics (or >1% proiotics) cn e utilized Diet-1 nd diet-2 were performed sttisticlly on pr in terms of optimized growth performnce of with control diet in terms of growth performnce regrding M.rosenergii. But diet with 2% proiotics is strongly ll growth prmeters except survivl nd FCR even nd recommended from the 2 experiment in ccordnce with though; the control diet ws highly proteinceous. As fr higher survivl nd economic efficiency due to cost s the price of the ll three diets were concerned, effectiveness thn 3% inclusion of proiotics Biosyn-AQ. experimentl diets (Diet-1: 138/= per kg nd Diet-2: 129/= Hence, control diet (commercil shrimp feed) nd per kg) were cheper thn control diet (250/=). Diet 1 ws diet-1 supplemented with proiotic Biosyn-AQ which performed etter thn diet-2 in terms of survivl nd FCR. showed improved growth performnce nd survivl of In selecting est diet from the experimentl diets, Diet-1 M.rosenergi in experiment-1 cn e used insted of incorported with proiotics Biosyn-AQ ws highly in currently used poultry feed strter in semi-intensive fresh greement with the performnce of protein rich control wter prwn nurseries nd grow-out ponds. From oth diet regrding ll the growth prmeters nd survivl nd experiments, It cn e concluded tht developing it is cheper thn the control diet s well. Then sl comintion of rtificilly formulted diet-1 with 2% mixture of diet-1 lone hs een undergone for second inclusion level of proiotics Biosyn-AQ cn e experiment in order to find out the optimum proiotic suggested for promoting sustinle culture of concentrtion tht leds to mximized growth performnce M.rosenergii under Sri Lnkn conditions in ccordnce of prwns. with etter growth prmeters nd low cost thus leding to higher profits. Growth Performnce: Experiment-2: Proiotics influence digestive processes y enhncing microil enzyme REFERENCES ctivity nd consequently improving the digestion, sorption nd utiliztion of the feed [46]. According 1. Aknd, A.M. nd Hsn, M.R, Sttus of to the results from experiment-2, Diets supplemented freshwter prwn (Mcrorchium spp.) culture in with 2% nd 3% proiotics fed juveniles showed Bngldesh. In: Freshwter Prwns (ed. y E.G. significntly higher men vlues for growth prmeters Sils), pp Kerl Agriculturl University, nd survivl nd therefore etter growth performnce Thrissur, Indi. thn diet supplemented with 1% proiotics fed juveniles. 2. Chen, S.M. nd J.C. Chen, Effects of ph on Higher survivl rtes of 92.5% for diet with 2% proiotics survivl, growth, molting nd feeding of gint nd 87.5% for diet w ith 3% proiotics when compred freshwter prwn Mcrorchium rosenergii. to diet with 1% proiotics (62.5%) were oserved. Aquculture, 218: Similr improved survivl hs een reported in 3. Hettlrchchi, A. nd M. Kulrtne, M. rosenergii PL fed with Biogen supplemented diets [8] Oservtions on smll scle pond culture of gint nd diets supplemented with different proiotics [7]. fresh wter prwn. Journl of Inlnd Fisheries, Survivl rtes of 80-90% for diets with 45% CP 4: supplemented with proiotic Binifitin TM [36], 80-92% for 4. D Armo, L.R. nd S.S. Sheen, Nutritionl diets (40% CP) supplemented with comined proiotics requirements, feed formultion nd feeding prctices [9], 80-90% for diets (40% CP) supplemented with for intensive culture of the freshwter prwn, L.sporogenes [10] were supported the present results. Mcrorchium rosenergii. Review of Fisheries Seenivsn et l. [36] reported tht the growth prmeters Science, 2: (survivl, weight gin, SGR, feed conversion efficiency 5. Tidwell, J.H., C. Coyle, S.D. Weiel nd J. Evns, nd protein efficiency rtio) were significntly (P<0.05) Effects nd interctions of stocking density nd higher in 2% Binifit TM incorported diet fed PL. Although dded sustrte on production nd popultion Seenivsn et l. [9] were suggested 3% L.sporogenes + structure of freshwter prwns Mcrorchium B.sutilis + S.cerevisie incorported diet fed post lrve rosenergii. Journl of the World Aquculture (PL) to otin significntly higher growth prmeters nd Society, 30: % L. sporogenes incorported diet ws recommended y 6. Gtesoupe, F.J., The use of proiotics in Seenivsn et l. [11] in order to ttined optimized quculture. Aquculture, 180:

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