Key words: Fusarium graminearum, Fusarium verticillioides, maize, fumonisin, mycotoxin, resistance, visual rating, 557 inoculation methods.
|
|
- Katherine Warner
- 6 years ago
- Views:
Transcription
1 548 Epidemiology / Épidémiologie Fumonisin B 1 accumulation and severity of fusarium ear rot and gibberella ear rot in food-grade corn hybrids in Ontario after inoculation according to two methods A.W. Schaafsma, L. Tamburic-Ilincic, and L.M. Reid Abstract: Fusarium verticillioides and Fusarium graminearum are pathogens of corn causing fusarium ear rot and gibberella ear rot, respectively. The mycotoxins fumonisin B 1 (FB 1 ) and deoxynivalenol (DON) are produced by F. verticillioides and F. graminearum, respectively, and commercial millers monitor the levels of these toxins in corn to avoid problems with contamination in finished foods. The objectives of the present study were: (1) to evaluate the susceptibility of corn hybrids to F. graminearum and F. verticillioides, following silk-channel or kernel-wounding inoculations, (2) to evaluate the variability in FB 1 production among food-grade corn hybrids, following silk or wound inoculation with F. verticillioides, and (3) to determine the association between the severity of fusarium ear rot and FB 1 to see if visual ratings of fusarium ear rot are predictive of the tendency of corn hybrids to accumulate FB 1. The experiments were conducted in the field from 1999 to 2002 with different sets of commercial hybrids each year. Mean fusarium ear rot severity and FB 1 accumulation varied among years regardless of the inoculation method. Severity of fusarium ear rot and gibberella ear rot (rating on a scale of 1 7) varied respectively from 1.5 to 2.7 and 1.8 to 3.9, with silk inoculation, and from 2.0 to 2.3 and 2.8 to 4.2, with wound inoculation. For a same inoculation method, hybrids responses differed between F. graminearum and F. verticillioides inoculations, likely influenced by the different environmental conditions favoured by each fungus. There was a high level of variation in FB 1 accumulation among the hybrids within each year; for example, FB 1 accumulation across hybrids grown under the same environmental conditions ranged from not detected to ppm in Severity of fusarium ear rot and FB 1 accumulation after wound inoculation with F. verticillioides were correlated, suggesting that severity symptoms of fusarium ear rot after wound inoculation might be useful to select hybrids that are less likely to accumulate FB 1. Key words: Fusarium graminearum, Fusarium verticillioides, maize, fumonisin, mycotoxin, resistance, visual rating, 557 inoculation methods. Résumé : Le Fusarium verticillioides et le Fusarium graminearum sont des agents pathogènes du maïs respectivement responsables de la fusariose de l épi et du grain et de la fusariose de l épi. Les mycotoxines fumonisine B 1 (FB 1 )et désoxynivalénol (DON) sont respectivement produites par le F. verticillioides et le F. graminearum, et les meuneries commerciales surveillent la concentration de ces toxines dans le maïs afin d éviter les problèmes reliés à la contamination des aliments transformés. Les objectifs de la présente étude étaient (1) d évaluer la sensibilité de maïs hybrides au F. graminearum et au F. verticillioides inoculés par voie des soies et par blessure des grains, (2) d évaluer la variabilité de la production de FB 1 parmi les hybrides de maïs de qualité alimentaire par suite d inoculations des soies ou par blessure avec le F. verticillioides et (3) de déterminer l association entre l intensité de la fusariose de l épi et du grain et la FB 1 de façon à déterminer si la notation visuelle de la fusariose de l épi et du grain permet de prédire la tendance des maïs hybrides à accumuler la FB 1. Des expériences furent menées sur le terrain de 1999 à 2002 avec différentes séries d hybrides commerciaux à chaque année. L intensité moyenne de la fusariose de l épi et du grain et l accumulation de la FB 1 varia selon l année, quelle que soit la méthode d inoculation. Avec l inoculation des soies, l intensité de la fusariose de l épi et du grain et de la fusariose de l épi (cote sur une échelle de 1à7)varia respectivement de 1,5 à 2,7 et de 1,8 à 3,9 et, avec l inoculation par blessure, de 2,0 à 2.3 et 2,8 à 4,2. Pour une même méthode d inoculation, la réponse des hybrides différait entre les inoculations avec le F. graminearum et Accepted 31 October A.W. Schaafsma 1 and L. Tamburic-Ilincic. University of Guelph, Ridgetown Campus, Ridgetown, ON N0P 2C0, Canada. L.M. Reid. Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, Ottawa, ON K1A 0C6, Canada. Contribution No from the Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada. 1 Corresponding author ( aschaafs@ridgetownc.uoguelph.ca). Can. J. Plant Pathol. 28: (2006)
2 Schaafsma et al.: corn / fusarium ear rot / gibberella ear rot / mycotoxins / inoculation methods 549 celles avec le F. verticillioides, vraisemblablement influencée par les différentes conditions environnementales privilégiées par chaque champignon. Il y avait une forte variation dans la teneur en FB 1 parmi les hybrides à l intérieur de chaque année; par exemple, dans les mêmes conditions environnementales, la teneur en FB 1 d un hybride à l autre alla d indétectable à 211,2 ppm en L intensité de la fusariose de l épi et du grain et la teneur en FB 1 après inoculation par blessure avec le F. verticillioides étaient corrélées, ce qui suggère que les symptômes d intensité de la fusariose de l épi et du grain après inoculation par blessure pourraient être utiles pour la sélection d hybrides moins susceptibles d accumuler de la FB 1. methods Mots clés : Fusarium graminearum, Fusarium verticillioides, maïs, fumonisine, mycotoxine, résistance, cote visuelle, méthodes d inoculation. Schaafsma et al.: corn / fusarium ear rot / gibberella ear rot / mycotoxins / inoculation Introduction Interest in expanding food-grade corn production in Canada to offset imports has grown in recent years (Doidge 1997). One of the main barriers to viable food-grade corn production is the potential for Fusarium toxins to accumulate in the grain. Early-maturing corn hybrids suitable for both the food industry and agriculture in Ontario were identified in a concurrent study (Darrah et al. 2001), which also provided the opportunity to examine these same hybrids for their reaction to Fusarium spp. and their propensity to accumulate mycotoxins. Fusarium graminearum Schwabe (teleomorph Gibberella zeae (Schw.) Petch) and Fusarium verticillioides (Sacc.) Nirenberg (= Fusarium moniliforme Sheldon) (teleomorph Gibberella fujikuroi (Sawada) Wr.) are species of Fusarium frequently isolated from corn ears in Ontario, Canada (Vigier et al. 1997). Fusarium graminearum causes gibberella ear rot and produces the mycotoxin deoxynivalenol (DON) in the grain, while F. verticillioides causes fusarium ear rot and produces the mycotoxin fumonisin B 1 (FB 1 ) (Nelson et al. 1993). Canada has no guidelines for either toxin in foodgrade corn, but both may cause potentially serious animal and human health problems (Miller and Trenholm 1994). Commercial millers monitor the levels of these toxins in corn to avoid problems with contamination in finished foods. Fumonisins and DON are found frequently in corn samples in Ontario. Fumonisins were recovered from naturally infected corn grown in Ontario for the first time in 1993 (Miller et al. 1995). Naturally contaminated corn samples from Eastern Canada have been reported to contain fumonisin concentration averaging 0.31 µg g 1 with a maximum of 1.71 µg g 1 (Campbell et al. 2002). In contrast, corn inoculated with F. verticillioides, using the silk method, contains much more fumonisin, with concentrations ranging from 4.0 µg g 1 to µg g 1 (Kleinschmidt et al. 2005). DON has been reported in 73% of the corn samples in Ontario at concentrations 0.2 µg g 1, and approximately 23% of the samples were contaminated with FB 1 at concentrations 1.0 µg g 1 (Hooker and Schaafsma 2005). Fusarium graminearum growth in Ontario is favoured by temperatures around 28 C and wet conditions (Sutton 1982; Miller et al. 1995), whereas F. verticillioides is favoured by temperatures 2 to 3 C higher and dry conditions (Miller et al. 1995; Reid et al. 1999). High incidence of FB 1 (58%) and DON (88%) in corn occurred in 1999, in Ontario, when near-normal rainfall and above-normal temperatures prevailed from mid-june to mid-october (Hooker and Schaafsma 2005). Significant interactions between genotype and environment were reported for fumonisin accumulation in Argentina (de la Campa et al. 2005). In the same study, insect damage significantly increased fumonisin production. However, hybrid-corn genetics had more impact than environment on DON and FB 1 accumulation in Ontario (Hooker and Schaafsma 2005). Selecting for genetic resistance of corn to F. graminearum may help mitigate the risk of mycotoxin contamination. However, Schaafsma et al. (1997) found that only a few hybrids exhibited consistent resistance to F. graminearum across environments, with wound inoculations and silk inoculations giving different results. They also reported that rating hybrids visually for severity of gibberella ear rot, without the high cost of DON analysis, may be appropriate if done over several years. Kleinschmidt et al. (2005) were the first to report on visually evaluating resistance of hybrids to F. verticillioides in relation to their propensity to accumulate fumonisins. They used only the silk-channel method of inoculation and found that the amount of visible fusarium ear rot after inoculation was not highly correlated with the amount of FB 1. Furthermore, these silk inoculations resulted in unnaturally high levels of FB 1 in some of the food-grade corn hybrids tested (Kleinschmidt et al. 2005). Fumonisin has been reported in asymptomatic kernels (Rheeder et al. 1992), and kernel wounding factored prominently in the contamination of grain with this toxin (de la Campa et al. 2005). The objectives of the present study were: (1) to determine if the susceptibilities of food-grade corn hybrids to fusarium ear rot and gibberella ear rot are correlated, following silkchannel or kernel-wounding inoculations, (2) to compare FB 1 concentrations after silk or wound inoculation with F. verticillioides, (3) to determine if fusarium ear rot severity and FB 1 accumulation are correlated, and (4) to determine a suitable method of inoculation as well as a suitable method of rating to screen corn hybrids for their tendency to accumulate FB 1. Materials and methods Experimental design and plant material Two similar experiments were conducted each year in early May in 1999, 2000, 2001, and 2002 at Ridgetown, Ontario; one for F verticillioides without mist irrigation and the other for F. graminearum with mist irrigation. Each experiment was designed as a split plot arranged in a randomized complete block design, with blocks replicated three
3 550 Can. J. Plant Pathol. Vol. 28, 2006 times. Main plots were hybrids and these were split into two equal subplots with one assigned to the silk-channel inoculation and the other to the kernel-wounding inoculation. Main plots were single rows spaced 0.76 m apart and 4 m long, with 35 seeds planted per plot. Subplots were one half of each main plot or 2 m rows. Different sets of commercial hybrids were planted each year, following the annual list for performance testing of food-grade hybrid-corn candidates (Darrah et al. 2001), and all hybrids were tested to each pathogen. One hybrid (Pioneer 34P93) was common to all 4 years of the study. Corn hybrids were planted in early May and thinned to 25 plants per plot during June of each year. The crop was maintained according to provincial recommendations for weed control and plant nutrition (Baute 2002). Inoculum preparation and inoculation Single-spore cultures of F. verticillioides (isolate DAOM ) and F. graminearum (isolate DAOM ) were generated and inoculum was produced according to Schaafsma et al. (1993). For the silk-inoculation method, a 2 ml aliquot of a suspension of F. graminearum or F. verticillioides macroconidia at a concentration of 10 5 conidia/ml was injected with a needle into the silk channels of individual ears (Reid et al. 1993). For the wound-inoculation method, the kernels were damaged directly via penetration of the husk with 25 brass pins embedded in a lead square measuring 2.5cm 2.5cm.A2mLaliquot of a suspension with 10 5 conidia/ml was sprayed directly into the wound area (Schaafsma et al. 1993). Ten primary ears of each hybrid were inoculated once per replicate, per inoculation method in each subplot, for each of the two pathogens. Silk inoculations were done at 50% silk browning (Reid et al. 1992), while wound inoculations were done after 600 crop heat units (Brown and Bootsma 1993) accumulated post 50% silking, when kernels were most susceptible to wound infection (Schaafsma et al. 1993; Reid et al. 2002). The single inoculations were spread over 3 weeks to accommodate the range of hybrid maturities. Plots inoculated with F. graminearum were irrigated using overhead misting (Schaafsma et al. 1997), from the first date of inoculation until 3 weeks after the last one for each year. Plots inoculated with F. verticillioides were not irrigated, to encourage dryer conditions more favourable for this fungus (Miller et al. 1995). Evaluation of severity of fusarium ear rot and gibberella ear rot Ears were rated for severity of fusarium ear rot and gibberella ear rot in mid-october of each year. For the silkchannel inoculation method, the percentage of kernels covered with fungal growth was rated according to a 1 7 scale (Reid et al. 1993): 1, 0%; 2, 1% 3%; 3, 4% 10%; 4, 11% 25%; 5, 26% 50%; 6, 51% 75%; 7, >75%. This scale was modified for disease-severity rating under the wound-inoculation method: 1 3, no girdling of the cob observed; 4 7 girdled cobs observed. When plots were inoculated with F. verticillioides, if the mycelium was white to salmon in colour, we assumed that fusarium ear rot was the disease manifested. When plots were inoculated with F. graminearum, if the mycelium showed pink, red, and purple colouration, we assumed that gibberella ear rot was manifested. An average severity rating was calculated per replication for each subplot in both experiments. Determination of FB 1 levels In the experiment with F. verticillioides, 10 ears from each plot were harvested at maturity for FB 1 analysis and threshed by hand in November of each year. Only the hybrids from the F. verticillioides experiment were used for FB 1 analysis. The grain from each plot was pooled and these single-plot samples were ground through a No. 60 grist mill (C.S. Bell Co., Tiffin, Ohio); the resulting meal was thoroughly mixed by hand. A 250 g subsample of meal was then ground finely through a grinding subsampling mill (model 2A, Romer Labs, Inc., Union, Mo.). Extractions were made from a 50 g sample of this flour, using 100 ml of acetonitrile (C 2 H 3 N) and 4% aqueous KCl in a ratio of 9:1 (v/v). Quantification of FB 1 was achieved under thin layer chromatography with a limit of detection of approximately 1.0 µg g 1 (Schaafsma et al. 2002). Statistics Data for minimum and maximum daily temperatures and rainfall were obtained from the Environment Canada automated weather station located at Ridgetown, Ontario, for 30 days after corn inoculation in each year and these were compared with the 30-year averages from the same weather station for the same period. Normality and homogeneity of variance for the data was tested using PROC UNIVARIATE version 8 (SAS Institute Inc. 1999), and the data were transformed using ln(x +1)as required. Analyses of variance with the general linear model (PROC GLM) were conducted by year (separately for the variables gibberella ear rot severity, fusarium ear rot severity, and FB 1 accumulation) because different corn hybrids were used each year. Hybrid response to the variables FB 1 accumulation, fusarium ear rot severity, and gibberella ear rot severity were compared using a Pearson s and Spearman s rank correlation coefficient (SAS Institute Inc. 1999) because the hybrids were tested in two different experiments. The level of significance used for statistical analyses was P= 0.05 unless otherwise stated. Results Environmental conditions varied among the 4 years of the study (Fig. 1). Average temperature and rainfall were near normal in 1999 and 2001 compared with the 30-year averages. In 2000, temperatures were lower while mean rainfall was much higher than the 30-year averages, and in 2002, temperatures were very high and rainfall was low. Gibberella ear rot severity, after silk inoculation with F. graminearum, ranged from 2.7 to 5.5, 2.1 to 5.5, 1.6 to 5.8, and 1.2 to 2.8 in 1999, 2000, 2001, and 2002, respectively. After wound inoculation, gibberella ear rot severity ranged from 2.9 to 5.7, 3.3 to 4.6, 2.1 to 4.7, and 1.8 to 3.7 in 1999, 2000, 2001, and 2002, respectively. Mean severity of gibberella ear rot after either silk or wound inoculation with F. graminearum was similar in 1999, 2000, and 2001, but lower in 2002 (1.8 and 2.8, respectively) (Table 1).
4 Schaafsma et al.: corn / fusarium ear rot / gibberella ear rot / mycotoxins / inoculation methods 551 Table 1. Gibberella ear rot severity in food-grade corn hybrids after silk or wound inoculation with Fusarium graminearum, and fusarium ear rot severity and fumonisin B1 (FB1) levels in food-grade corn hybrids after silk or wound inoculation with Fusarium verticillioides, at Ridgetown, Ontario, Disease severity a Gibberella ear rot Fusarium ear rot Concentration of FB 1 (ppm) d Hybrid Silk b Wound c Silk b Wound c Silk Wound 1999 Dekalb DK Dekalb DK Dekalb DK Direct D Direct 9979xFG Enerfeast Garst 8527W Maizex dry Maizex dry Maizex wet Maizex wet Mycogen X Novartis G Novartis MAX Novartis MAX Novartis MAX Novartis N47-H Novartis NX Novartis Wilson Pioneer 34K Pioneer 34P Pioneer 3443W Pioneer Pioneer Pioneer 36B Pioneer 36K Pioneer 36Y Pioneer 36Y Pioneer Pioneer Pride K Pride K Pride L-ZM 548/ Pride L-ZM 548/ Renk RK Renk RK Renk RK Mean Coefficient of variation Great Lakes Hyland HL NK E Pioneer 34K Pioneer 34P Pride K Pride X Pride X Mean Coefficient of variation Asgrow RX776W
5 552 Can. J. Plant Pathol. Vol. 28, 2006 Table 1 (concluded). Disease severity a Gibberella ear rot Fusarium ear rot Concentration of FB 1 (ppm) d Hybrid Silk b Wound c Silk b Wound c Silk Wound B73 Mo IFSI Lfy (FR810 Lfy728w) Lfy (MBS62W Lfy728W) Monsanto EXP 162W Nd NC+ RE557W Pioneer 32H Pioneer 32K Pioneer d 33T Pioneer Pionner 34P Pioneer Vineyard V420W Vineyard V431W Vineyard V433W Vineyard V445W Vineyard Vx6122W Whisnand 50AW Whisnand 100W Nd Nd Zimmerman 1790W Zimmerman E Zimmerman E Zimmerman Z75W Mean Coefficient of variation Asgrow RX776W Guelph CGX004W Guelph CGX010W Guelph CGX013W Monsanto EXP 162W NC+ 4950W NC+ 5642W Pioneer 32A Pioneer 32K Pioneer 33T Pioneer 34P Pioneer Brand 32W Pioneer Brand Vineyard V433W Vineyard Vx7120W Whisnand 50AW Whisnand 100W Yellow check B73 Mo Zimmerman 1790W Zimmerman WX Mean Coefficient of variation Note: Nd, not detected. a Rated on a scale of 1 to 7, according to the percentage of kernels that were covered with mold: 1, 0%; 2, 1% 3%; 3, 4% 10%; 4, 11% 25%; 5, 26% 50%; 6, 51% 75%; 7, >75%. b A macroconidial suspension (2 ml) of F. graminearum or F. verticillioides at a concentration of 10 5 conidia/ml was injected into the silk channels of individual ears with a syringe. c The kernels were directly damaged via penetration of the husk with 25 brass pins embedded in a lead square measuring 2.5 cm 2.5 cm, previously dipped in a conidial suspension of F. graminearum or F. verticillioides, delivering 2 ml of a suspension with 10 5 conidia/ml into the wound area. d Transformed using ln(x + 1).
6 Schaafsma et al.: corn / fusarium ear rot / gibberella ear rot / mycotoxins / inoculation methods 553 Fig. 1. Mean temperature and cumulative rainfall for the 30 days after corn inoculation in , and the 30-year averages based on data from June and July in each year, at Ridgetown, Ontario. Fig. 2. Disease severity (on a scale of 1 to 7) + standard error in food-grade corn hybrid Pioneer 34P93 after silk or wound inoculation at Ridgetown, Ontario, Gibberella ear rot severity was rated after inoculation with Fusarium graminearum, and fusarium ear rot severity was rated after inoculation with Fusarium verticillioides. Fig. 3. Fumonisin B 1 accumulation + standard error in the foodgrade corn hybrid Pioneer 34P93 after silk or wound inoculation with Fusarium verticillioides at Ridgetown, Ontario Fusarium ear rot severity, after silk inoculation with F. verticillioides, ranged from 1.0 to 2.1, 1.8 to 4.7, 1.1 to 4.2, and 1.1 to 2.2 in 1999, 2000, 2001, and 2002, respectively. After wound inoculation, fusarium ear rot severity ranged from 1.1 to 3.2, 2.0 to 2.8, 1.4 to 3.5, and 1.5 to 3.5 in 1999, 2000, 2001, and 2002, respectively. Fusarium ear rot severity in Pioneer 34P93 was consistently higher across all years after wound inoculation than after silk inoculation (Fig. 2). The highest fusarium ear rot severity (4.7) across all years was found in hybrid Hyland HL2632 in 2000 after silk inoculation (Table 1). FB 1 levels, after silk inoculation with F. verticillioides, ranged from 2.0 to ppm, 1.0 to 14.0 ppm, not detected to 28.3 ppm, and 1.7 to 33.4 ppm in 1999, 2000, 2001, and 2002, respectively (Table 1). After wound inoculation, FB 1 levels ranged from 7.3 to ppm, 1.3 to 10.0 ppm, not detected to ppm, and 7.2 to 85.6 ppm in 1999, 2000, 2001, and 2002, respectively. The highest mean concentration of FB 1 across all years and hybrids, after both methods of inoculation (15.8 and 51.7 ppm, respectively), occurred in 1999 (Table 1). Generally hybrids had higher mean levels of FB 1 after wound inoculation than after silk inoculation in all years, except in 2000 (Table 1). In addition, the FB 1 concentration in hybrid Pioneer 34P93 across the years was also higher after wound inoculation than after silk inoculation, except in 2000 (Fig. 3). In 1999, Pioneer 36Y96 had the highest level of FB 1 after silk inoculation, while Pioneer 36B08 had the highest level of FB 1 after wound inoculation (Table 1). After silk inoculation, Pioneer 36Y96 had low fusarium ear rot severity but high FB 1 level in In contrast, Dekalb DK579 had low fusarium ear rot severities and FB 1 levels after wound or silk inoculation in 1999 (Table 1).
7 554 Can. J. Plant Pathol. Vol. 28, 2006 Table 2. Analysis of variance for the effect of the hybrid, the method of inoculation, and their interaction on gibberella ear rot severity in food-grade corn hybrids after silk or wound inoculation with Fusarium graminearum and on fusarium ear rot severity and fumonisin B1 (FB 1 ) levels in food-grade corn hybrids after silk or wound inoculation with Fusarium verticillioides, at Ridgetown, Ontario, df Mean square F P >F a Severity of gibberella ear rot 1999 Hybrid (H) Method (M) H M Hybrid Method H M Hybrid Method H M Hybrid Method H M Severity of fusarium ear rot 1999 Hybrid Method H M Hybrid Method H M Hybrid Method H M Hybrid Method H M FB 1 level 1999 Hybrid Method H M Hybrid Method H M Hybrid Method H M Hybrid Method Table 2 (concluded). df Mean square F P >F a H M Note: The analysis of variance was conducted separately for severity of gibberella ear rot, severity of fusarium ear rot, and FB 1 accumulation. df, degree of freedom. a The level of significance for the statistical analyses was P = The lowest mean level of FB 1 across years and hybrids occurred in 2000 for silk and wound inoculations combined (8.0 and 6.3 ppm, respectively) (Table 1). There was a high level of variation in FB 1 accumulation among the hybrids (from not detected to ppm after wound inoculation) in In the same year, hybrid Lfy (FR810 Lfy728w) had the highest detected level (211.2 ppm) of FB 1 across years and hybrids (Table 1). Novartis G-4030 had high FB 1 levels after wound inoculation but not after silk inoculation (80.0 vs. 2.0 ppm) in 1999 (Table 1). There was no FB 1 detected in hybrid Whisnand 100W after both methods of inoculation in 2001 (Table 1). However, in 2002, Whisnand 100W had 19.8 and 42.2 ppm of FB 1 after silk and wound inoculations, respectively (Table 1). A comparison across all years of hybrid performance of Pioneer 34P93 showed that gibberella ear rot severity was higher than fusarium ear rot severity for all growing seasons except for 2001, when fusarium ear rot severity after wound inoculation was higher. Fusarium ear rot severity was more severe after wound inoculation than silk inoculation with the opposite for gibberella ear rot severity, except in 2002 (Fig. 2). In addition, FB 1 accumulation was increased about twofold by wound inoculation compared with silk inoculation, except for the 2000 growing season (Fig. 3). Hybrids and the method of inoculation significantly influenced fusarium ear rot severity in all years and the gibberella ear rot severity and FB 1 accumulation in 1999, 2001, and 2002 (Table 2). There was a significant interaction between hybrids and the method of inoculation for fusarium ear rot severity in all years, with the exception of 2002 (Table 5). This interaction was also significant for FB 1 accumulation in 2001 and for gibberella ear rot severity in 2001 and 2002 (Table 2). Pearson s and Spearman s rank correlations were calculated and found to be similar, therefore, only Spearman s rank correlation coefficient is presented in Table 3. Data on gibberella ear rot severity after silk or wound inoculation were correlated after inoculation with F. graminearum in all years, except in 2002 (Table 3). Data on fusarium ear rot severity after silk or wound inoculation with F. verticillioides were correlated in 2000 and 2002, but not in 1999 and 2001 (Table 3). FB 1 levels after both inoculation methods were significantly correlated within the years, with the exception of 2000 (Table 3). There was a significant correlation between fusarium ear rot severity and FB 1 accumulation after silk inoculation in all years, except in 2001 (Table 3). However, the highest and most consistent correlation among all variables tested was between fusarium ear rot severity and FB 1 accumulation after wound inoculation (r = 0.51, 0.63, 0.73, and 0.78 in 1999, 2000, 2001, and 2002, respectively) (Table 3).
8 Schaafsma et al.: corn / fusarium ear rot / gibberella ear rot / mycotoxins / inoculation methods 555 Table 3. Spearman s correlation coefficients among gibberella ear rot severity in foodgrade corn hybrids after silk or wound inoculation with Fusarium graminearum, fusarium ear rot severity, and fumonisin B 1 (FB 1 ) levels in food-grade corn hybrids after silk and wound inoculation with Fusarium verticillioides, at Ridgetown, Ontario, Gibberella ear rot Fusarium ear rot FB 1 Silk Wound Silk Wound Silk 1999 Fumonisin B 1 Wound NS NS 0.32* 0.51** 0.52** Silk NS NS 0.49** 0.36* Fusarium ear rot Wound NS NS NS Silk NS NS Gibberella ear rot Wound 0.48** 2000 Fumonisin B 1 Wound 0.65* 0.81* NS 0.63** NS Silk NS NS 0.66* NS Fusarium ear rot Wound NS NS 0.69* Silk NS NS Gibberella ear rot Wound 0.69* 2001 Fumonisin B 1 Wound NS NS NS 0.73** 0.37* Silk NS NS NS NS Fusarium ear rot Wound NS NS NS Silk NS NS Gibberella ear rot Wound 0.71** 2002 Fumonisin B 1 Wound NS 0.45* 0.56* 0.78** 0.66** Silk NS 0.60* 0.81** 0.62** Fusarium ear rot Wound NS 0.40* 0.47* Silk NS 0.75** Gibberella ear rot Wound NS Note: *, P < 0.05; **, P < ; NS, not significant. Discussion Disease severities were greater for both fungi in 1999 and 2001, when average temperatures and rainfall were near normal compared with the 30-year averages (Fig. 1). Both F. verticillioides and F. graminearum reach maximum growth around 28 C, however, a decline was observed for F. graminearum at higher temperatures, but not for F. verticillioides (de la Campa et al. 2005). In 2000, growth of Fusarium graminearum was favoured over that of F. verticillioides by lower temperatures and higher rainfall than the 30-year averages (Fig. 1). In 2002, in contrast, F. verticillioides growth and FB 1 accumulation were favoured over those of F. graminearum by higher temperatures and lower rainfall than the 30-year averages. Other studies (Sutton 1982; Miller et al. 1995; Reid et al. 1999; de la Campa et al. 2005) have also found that higher rainfall stimulates F. graminearum growth, while hot and dry conditions stimulate F. verticillioides growth and FB 1 accumulation. This study was not designed to compare hybrids across environments and to study genotype environment interactions. However, ear rot severity and FB 1 accumulation varied among years regardless of inoculation method. In addition, hybrids and the method of inoculation significantly influ-
9 556 Can. J. Plant Pathol. Vol. 28, 2006 enced fusarium ear rot severity in all years and the gibberella ear rot severity and FB 1 accumulation in 1999, 2001, and These results emphasize the complexities involved with hybrid screening as affected by the different environments experienced and hybrids used during the 4 years of this study. This emphasizes the need to screen for resistance to gibberella ear rot, fusarium ear rot, and (or) fumonisin accumulation under specific targeted conditions. The range in gibberella ear rot severity produced with inoculation of F. graminearum on food-grade corn hybrids are consistent with those found earlier in commodity corn hybrids (Schaafsma et al. 1993). Moreover, gibberella ear rot severity after silk or wound inoculation with F. graminearum correlated well in all years, except in We assume that hot and dry weather conditions experienced in 2002 likely stressed and shortened silking, providing unfavourable conditions for F. graminearum propagules to germinate and grow in the silk-channel method. The woundinoculation method is less dependent on environment in that the inoculum is introduced directly into wounded, developing kernels that have high water potential. The hybrids with high gibberella ear rot severity did not necessarily have high fusarium ear rot severity, suggesting that the mechanisms for resistance to each pathogen responsible for these disease are likely different, or the disease may be affected by the different environmental conditions that favour F. graminearum and F. verticillioides growth. Our data suggest that screening for resistance to each type of ear rot ought to be done separately, using the appropriate species of Fusarium under optimized conditions for each species. Canada has no recommended tolerance levels for fumonisins, but the United States Food and Drug Administration recommends 2 to 4 µg g 1 or less for corn and corn products destined for human consumption (Kleinschmidt et al. 2005). FB 1 varied from not detected to 211 ppm in the current study. It was detected in most of the hybrids across all years of the study and its levels were generally higher in our inoculated experiments than those reported from naturally infected corn, which, according to Hooker and Schaafsma (2005), ranged from 0 to 7 ppm with a mean of 1.5 ppm in Ontario from 1993 to However, the high levels of FB 1 in our inoculated plots were similar to those reported by Kleinschmidt et al. (2005) where they ranged from 4 to 255 µg g 1, after silk inoculation. FB 1 levels for both inoculation methods were correlated across the years of this study, with the exception of 2000, when temperatures were significantly lower while rainfall was much higher than the 30-year averages. The hybrid list tested in 2000 was also smaller than for the other years, which may help to explain why there was no correlation in this year. The correlation between fusarium ear rot severity and FB 1 accumulation after silk inoculation was consistently low throughout all years of this study, supporting the finding of Kleinschmidt et al. (2005) who suggested that disease severity cannot be used to judge the tendency of corn hybrids to accumulate FB 1 when the silk-inoculation technique is applied. For example, we found that the hybrid Pioneer 36Y96 had low fusarium ear rot severity but high FB 1 level after silk inoculation in Furthermore, high concentrations of FB 1 arising from systemic infections with F. verticillioides can be detected in asymptomatic kernels (Rheeder et al. 1992). Fusarium ear rot severity and FB 1 accumulation after wound inoculation with F. verticillioides were consistently correlated. This relationship was not tested by Kleinschmidt et al. (2005). Therefore, fusarium ear rot symptoms after wound inoculation may be a useful criterion to screen hybrids for their resistance to FB 1 accumulation, perhaps with the goal of keeping the highly susceptible hybrids out of the foodproduction chain. Robertson et al. (2006) also recommended, on the basis of genetic correlation and the employment of wound inoculations 1 week after silk inoculations, that hybrids be screened visually for ear rot without the high cost of FB 1 analysis. In conclusion, this work emphasizes the importance of screening for resistance to fusarium ear rot and gibberella ear rot separately, because the causal organisms respond quite differently to hybrids and the environment. Gibberella ear rot symptoms may be used as a surrogate predictor for DON accumulation after either silk or wound inoculation, as noted in previous studies. However, this is not the case for fusarium ear rot and FB 1 accumulation, where only ear rot severity after wound inoculation is predictive of FB 1. Finally, there is a measurable variability in susceptibility to fumonisin accumulation among food-grade corn hybrids, which is a promising avenue, and that breeding for resistance constitutes a viable path to take. Acknowledgements The authors thank two anonymous reviewers for their useful suggestions. They also want to extend their appreciation to the Ontario Corn Producers Association, Guelph, Ont.; the Ontario Ministry of Agriculture, Food and Rural Affairs; Pioneer Hi-bred Canada Ltd.; and the Canadian Adaptation Council for their financial support. Laboratory and field technical support by Diane Paul and Todd Phibbs of Ridgetown Campus, University of Guelph, Ontario, are gratefully acknowledged. References Baute, T Agronomy guide for field crops. Ontario Ministry of Agriculture, Food and Rural Affairs, Publication 811. Queens Printer, Toronto, Ont. Brown, D.M., and Bootsma, A Crop heat units for corn and other warm-season crops in Ontario. Ontario Ministry of Agriculture, Food and Rural Affairs, Factsheet No , Agdex 111/31. Queens Printer, Toronto, Ont. Campbell, H., Choo, T.M., Vigier, B., and Underhill, L Comparison of mycotoxin profiles among cereal samples from eastern Canada. Can. J. Bot. 80: Darrah, L.L., West, D.R., Lundquist, R.L., Hibbard, B.E., Wilmot, D.B., Schaafsma, A., Lee, E.A. Mckinney, J., Pearce, W.L., Betran, F.J., Xu, W., Michener, P.M., Pataky, J.K., Gordon, B., Maddux, L.D., Elmore, R.W., Stenburg, D.E., Beauzay, P.B., Thomison, P.R., Geyer, A.B., Minyo, R.J., Ziegler, K.E., Henry, R., Fischer, D.B., Strissel, J.F., Tragesser, S., and Duetsch, J.A White food-grade corn performance tests. Agricultural Research Service, US Department of Agriculture, Agricultural Experiment Station, University of Missouri-Columbia, Mo., Special Report 540.
10 Schaafsma et al.: corn / fusarium ear rot / gibberella ear rot / mycotoxins / inoculation methods 557 de la Campa, R., Hooker, D.C., Miller, J.D., Schaafsma, A.W., and Hammond, B.G Modeling effects of environment, insect damage, and Bt genotypes on fumonisin accumulation in maize in Argentina and Philippines. Mycopathologia, 159: Doidge, B.R Food grade corn: expanding the industry in Ontario. Internal report to the Ontario Corn Producers Association, Guelph, Ont. Hooker, D.C., and Schaafsma, A.W Agronomic and environmental impacts on concentrations of deoxynivalenol and fumonisin B 1 in corn across Ontario. Can. J. Plant Pathol. 27: Kleinschmidt, C.E., Clements, M.J., Maragos, C.M., Pataky, J.K., and White, D.G Evaluation of food-grade dent corn hybrids for severity of fusarium ear rot and fumonisin accumulation in grain. Plant Dis. 89: Miller, J.D., and Trenholm, H.L. (Editors) Mycotoxins in grains: compounds other than aflatoxin. Eagan Press, St. Paul, Minn. Miller, J.D., Savard, M.E., Schaafsma, A.W., Seifert, K.A., and Reid, L.A Mycotoxin production by Fusarium moniliforme and Fusarium proliferatum from Ontario and occurrence of fumonisin in the 1993 corn crop. Can. J. Plant Pathol. 17: Nelson, P.E., Desjardins, A.E., and Plattner, R.D Fumonisins, mycotoxins produced by Fusarium species: biology, chemistry, and significance. Annu. Rev. Phytopathol. 31: Reid, L.M., Mather, D.E., Hamilton, R.I., and Bolton, A.T Genotypic differences in the plant resistance of maize silk to Fusarium graminearum. Can. J. Plant Pathol. 14: Reid, L.M., Spaner, D., Mather, D.E., Bolton, A.T., and Hamilton, R.I Resistance of maize hybrids and inbreds following silk inoculation with three isolates of Fusarium graminearum. Plant Dis. 77: Reid, L.M., Nicol, R.W., Ouellet, T., Savard, M., Miller, J.D., Young, J.C., Stewart, D.W., and Schaafsma, A.W Interaction of Fusarium graminearum and F. moniliforme in maize ears: disease progress, fungal biomass, and mycotoxin accumulation. Phytopathology, 89: Reid, L.M., Woldemariam, T., Zhu, X., Stewart, D.W., and Schaafsma, A.W Effect of inoculation time and point of entry on disease severity in Fusarium graminearum, Fusarium verticillioides, orfusarium subglutinans inoculated maize ears. Can. J. Plant Pathol. 24: Rheeder, J.P., Marasas, W.F.O., Thiel, P.G., Syndenham, E.W., Shephard, G.S., and Schalkwyk, D.J.V Fusarium moniliforme and fumonisins in corn in relation to human esophageal cancer in Transkei. Phytopathology, 82: Robertson, L.A., Kleinschmidt, C.E., White, D.G., Payne, G.A., Maragos, C.M., and Holland, J.B Heritabilities and correlations of fusarium ear rot resistance and fumonisin contamination resistance in two maize populations. Crop Sci. 46: SAS Institute Inc SAS/STAT user s guide. Version 8. SAS Institute Inc., Cary, N.C. Schaafsma, A.W., Miller, J.D., Savard, M.E., and Ewing, R.J Ear rot development and mycotoxin production in corn in relation to inoculation method, corn hybrid and species of Fusarium. Can. J. Plant Pathol. 15: Schaafsma, A.W., Nicol, R.W., and Reid, L.M Evaluating commercial maize hybrids for resistance to gibberella ear rot. Eur. J. Plant Pathol. 103: Schaafsma, A.W, Hooker, D.C., Baute, T.S., and Tamburic- Ilincic, L Effects of Bt-corn hybrids on deoxynivalenol content in grain in harvest. Plant Dis. 86: Sutton, J.C Epidemiology of wheat head blight and maize ear rot caused by Fusarium graminearum. Can. J. Plant Pathol. 4: Vigier, B., Reid, L.M., Seifert, K.A., Stewart, D.W., and Hamilton, R.I Distribution and prediction of Fusarium species associated with maize ear rot in Ontario. Can. J. Plant Pathol. 19:
EAR AND KERNEL ROTS. When to look for: Mid-August to October (and during storage)
EAR AND KERNEL ROTS When to look for: Mid-August to October (and during storage) Reduction in yield and grain quality due to infection by fungi of ears and kernels both during the growing season, and while
More informationEffect of time of harvest on the incidence of Fusarium spp. in kernels of silage corn
Effect of time of harvest on the incidence of Fusarium spp. in kernels of silage corn Allen G. Xue 1, Baoluo L. Ma 1, Randall M. Clear 2, and Bernard J. Vigier 1 Received 2005-08-22; accepted 2005-12-12
More informationmycotoxin-contaminated contaminated food or feed
Poisons in our Food By Melvin Newman, Ph.D. Professor Entomology and Plant Pathology Foods that may contain toxins Introduction Fungi: produce an array of chemical products called secondary metabolites.
More informationEffect of Plant Height on Fusarium Head Blight in Spring Wheat
Journal of Plant Studies; Vol. 4, No. 2; 2015 ISSN 1927-0461 E-ISSN 1927-047X Published by Canadian Center of Science and Education Effect of Plant Height on Fusarium Head Blight in Spring Wheat Hana Moidu
More informationFusarium species and Deoxynivalenol in maize product of Moqan region
DOI: http://dx.doi.org/10.22092/jaep.2012.107219 Fusarium species and Deoxynivalenol in maize product of Moqan region - - ( : : ) n.aliakbari@gmail.com. -... (DON). Czapek PDA Nash-Snyder CLA PDA. % /
More informationDeoxynivalenol: Known Facts and Research Questions. DON (deoxynivalenol) is a damaging toxin produced by the fungus Fusarium
Deoxynivalenol: Known Facts and Research Questions Summary: DON (deoxynivalenol) is a damaging toxin produced by the fungus Fusarium graminearum in the heads of small grains. In addition to DON, F. graminearum
More informationFusaria and Fusarium mycotoxins in leaves and ears of maize plants 2. A time course study made in the Waikato region, New Zealand, in 1997
New Zealand Journal of Crop and Horticultural Science ISSN: 0114-0671 (Print) 1175-8783 (Online) Journal homepage: http://www.tandfonline.com/loi/tnzc20 Fusaria and Fusarium mycotoxins in leaves and ears
More informationREPORT TO THE AGRICULTURAL RESEARCH FOUNDATION FOR THE OREGON PROCESSED VEGETABLE COMMISSION December 2010 Project Title: Management of Fusarium
REPORT TO THE AGRICULTURAL RESEARCH FOUNDATION FOR THE OREGON PROCESSED VEGETABLE COMMISSION December 21 Project Title: Management of Fusarium diseases of sweet corn in the PNW: microflora influence on
More informationBulgarian Journal of Agricultural Science, 14 (No 3) 2008, Agricultural Academy
321 Bulgarian Journal of Agricultural Science, 14 (No 3) 2008, 321-328 Agricultural Academy INFLUENCE OF SOME AGRONOMY FACTORS ON SPIKE COMPONENTS AFTER A RARE INCIDENCE OF FUSARIUM HEAD BLIGHT EPIPHYTOTY
More information2008 PMR REPORT #ESOYSMI4 SECTION E: CEREAL, FORAGE CROPS, and OILSEEDS Insect Pests
2008 PMR REPORT #ESOYSMI4 SECTION E: CEREAL, FORAGE CROPS, and OILSEEDS Insect Pests CROP: Soybean, Glycine max (L.) Merr., Hyland Seed cvs. HS24R45 (3200 CHU) ( st planting) and RR Razor (2 nd planting)
More informationFOOD SAFETY, TOXICOLOGY AND UTILIZATION OF MYCOTOXIN-CONTAMINATED GRAIN
FOOD SAFETY, TOXICOLOGY AND UTILIZATION OF MYCOTOXIN-CONTAMINATED GRAIN Section 3: Food Safety, Toxicology, and Utilization of Mycotoxin-contaminated Grain WHEAT KERNEL BLACK POINT AND FUMONISIN CONTAMINATION
More informationAsian Journal of Agriculture and Rural Development. Screening of Mycotoxin Produced by Fusarium Verticillioides and F. Proliferatum in Culture Media
Asian Journal of Agriculture and Rural Development journal homepage: http://www.aessweb.com/journals/5005 Screening of Mycotoxin Produced by Fusarium Verticillioides and F. Proliferatum in Culture Media
More informationSection 4: Milling and Baking Quality
Section 4: Milling and Baking Quality Grain samples of 62 entries in Virginia s 2014 State Wheat Test grown at Blacksburg, VA were submitted to the USDA-ARS Soft Wheat Quality Lab in Wooster, OH for advanced
More informationRole of Seed Analysts in the Management of Scab Disease
Role of Seed Analysts in the Management of Scab Disease Dr. Bill Grey, MSU Foundation Seed Program Harold Armstrong, RST, Monsanto Corp. Association of Official Seed Analysts, Inc. / Society Commercial
More informationFusarium stalk rot of sorghum in the Northern region. By Lisa Keller and Malcolm Ryley, Agri-Science Qld, DEEDI, 203 Tor St Toowoomba
Fusarium stalk rot of sorghum in the Northern region By Lisa Keller and Malcolm Ryley, Agri-Science Qld, DEEDI, 203 Tor St Toowoomba Sorghum stalk rots are a continual but increasing threat to Australian
More informationAflatoxin Accumulation in a Maize Diallel Cross
Agriculture 2015, 5, 344-352; doi:10.3390/agriculture5020344 Article OPEN ACCESS agriculture ISSN 2077-0472 www.mdpi.com/journal/agriculture Aflatoxin Accumulation in a Maize Diallel Cross W. Paul Williams
More informationMany species in the genus Fusarium have the capacity to
Published September, 01 RESEARCH Diallel Analysis of Resistance to Fusarium Ear Rot and Fumonisin Contamination in Maize Hsiao-Yi Hung and James B. Holland* ABSTRACT The fungus Fusarium verticillioides
More informationJournal of Plant Pathology (2009), 91 (2), Edizioni ETS Pisa, AND AFLATOXIN B 1
023_JPP375SCAlakonya_459 25-06-2009 14:03 Pagina 459 Journal of Plant Pathology (2009), 91 (2), 459-464 Edizioni ETS Pisa, 2009 459 SHORT COMMUNICATION FUMONISIN B 1 AND AFLATOXIN B 1 LEVELS IN KENYAN
More informationTable 1 Disease Ratings* May 22 May 30 Tst Treatment and rate/a Inc Sev Fld Sev Inc Sev Fld Sev Bu/A** LSD P=
MANAGEMENT OF FUSARIUM HEAD BLIGHT IN WHEAT USING SELECTED BIOLOGICAL CONTROL AGENTS AND FOLIAR FUNGICIDES, 2001 D.E. Hershman 1, P.R. Bachi 1, D.M. TeKrony 2 and D.A. VanSanford 2 Department of Plant
More informationDOI: /v
P L A N T B R E E D I N G A N D S E E D S C I E N C E Volume 63 2011 DOI: 10.2478/v10129-011-0021-2 Vesna Krnjaja 1, Jelena Lević 2, Slavica Stanković 2, Zorica Bijelić 1 1 Institute for Animal Husbandry,
More informationReduced Fusarium Ear Rot and Symptomless Infection in Kernels of Maize Genetically Engineered for European Corn Borer Resistance
Disease Control and Pest Management Reduced Fusarium Ear Rot and Symptomless Infection in Kernels of Maize Genetically Engineered for European Corn Borer Resistance G. P. Munkvold, R. L. Hellmich, and
More informationSection 5: Wheat Scab Research
Section 5: Wheat Scab Research One of the primary research objectives of the Virginia Tech wheat breeding program is to identify and develop cultivars possessing resistance to Fusarium Head Blight () or
More informationSorghum Grain Mold: Variability in Fungal Complex
Table 2. Incidence and intensity of grain mold in sorghum cultivars under two treatments of panicles (with and without bags) 1. Incidence (%) Cultivar TGMR 2 Germination' Fusarium moniliforme 3 Curvularia
More informationMATERIALS AND METHODS 68
emr00 ACSJCA JCA10.0.1408/W Unicode research.3f (R2.0.i4 HF02:2745 2.0 alpha 36) 2011/11/15 15:21:00 PROD-JCA1 rq_38148 12/19/2011 15:15:15 5 pubs.acs.org/jafc 1 Survey of Mycotoxins in U.S. Distiller
More informationSignificant occurrence of aflatoxin M1 and M2 in milk, 2012
Eszterágpuszta : GOSZ maize test, ear rot data of maize hybrids GOSZ maize ear rot tests, means across eight locations, natural infection,. Aspergillus flavus as a new resistance problem in maize (Zea
More informationAbstract. Introduction
Pathogenicity and fusaric acid production by Fusarium proliferatum isolated from garlic in Spain D. PALMERO (1), M. DE CARA (2), W. NOSIR (3), C. IGLESIAS (1), M. GARCÍA (1), S. WOODWARD (3), J.C. TELLO
More informationAlternative Methods for the Control of Mycotoxins
Alternative Methods for the Control of Mycotoxins John F. Leslie University Distinguished Professor & Head Department of Plant Pathology Kansas State University Manhattan, Kansas 2014 J. F. Leslie What
More informationMycotoxins, MRL s & food chain contaminations
1 Mycotoxins, MRL s & food chain contaminations IAOM-MEA, 15.11.2008, Arusha Ir. Johny Boerjan Hazards associated with wheat & wheat processing (HACCP) Physical hazards Glass, stones, metal, Biological
More informationBurt Bluhm Department of Plant Pathology University of Arkansas Division of Agriculture
Burt Bluhm Department of Plant Pathology University of Arkansas Division of Agriculture Aspergillus flavus is a fungus that infects corn ears Produces aflatoxin causes cancer, other illnesses in humans
More informationDIAGNOSTIC VOMITOXIN (DON) SERVICES IN
DIAGNOSTIC VOMITOXIN (DON) SERVICES IN 2000-2001 Howard H. Casper* Department of Veterinary and Microbiological Sciences, North Dakota State University, Fargo, ND, 58105 *Corresponding author: PH: (701)
More informationSHORT COMMUNICATION OCCURRENCE AND DISTRIBUTION OF FUSARIUM SPP. ASSOCIATED WITH DURUM WHEAT SEED FROM ARGENTINA
Journal of Plant Pathology (2001), 83 (1), 63-67 Edizioni ETS Pisa, 2001 63 SHORT COMMUNICATION OCCURRENCE AND DISTRIBUTION OF FUSARIUM SPP. ASSOCIATED WITH DURUM WHEAT SEED FROM ARGENTINA G.A. Lori and
More informationMANAGEMENT OF FUSARIUM HEAD BLIGHT OF WHEAT USING ANTAGONISTIC MICROORGANISMS
MANAGEMENT OF FUSARIUM HEAD BLIGHT OF WHEAT USING ANTAGONISTIC MICROORGANISMS *Muthomi, J. W., Riungu, G. M., and Wagacha, J. M. Department of Plant Science and Crop Protection, University of Nairobi P.
More informationA new race of Diplocarpon rosae capable of causing severe black spot on Rosa rugosa hybrids
I 38 lnventaire des maladies des plantes au Canada 59:2, 1979 A new race of Diplocarpon rosae capable of causing severe black spot on Rosa rugosa hybrids A. T. Bolton and F. J. Svejda In 1977, severe black
More informationFumonisins are a significant health risk to livestock, and potentially also to humans , B 2
Food Safety Digest February 2018 REF. No.: WHO/NHM/FOS/RAM/18.2 Department of Food Safety and Zoonoses Fumonisins Fumonisins are a significant health risk to livestock, and potentially also to humans Fumonisins
More informationABSTRACT. Fusarium ear rot, caused by Fusarium verticillioides (Sacc.) Nirenberg (Syn. = F.
ABSTRACT HORNE, DAVID WILLIAM. Effects of Three Generations of Recurrent Index Selection for Fusarium Ear Rot Resistance, Grain Yield, and Lodging Resistance in Maize. (Under the direction of Dr. James
More informationRESEARCH REPOSITORY.
RESEARCH REPOSITORY This is the author s final version of the work, as accepted for publication following peer review but without the publisher s layout or pagination. The definitive version is available
More informationMYCOTOXINS COMMONLY FOUND IN IOWA
MYCOTOXINS COMMONLY FOUND IN IOWA The purpose of this paper is to provide information about mycotoxins commonly found in Iowa, including their sources, conditions favoring production, health effects, and
More informationEffect of Nozzles on Fungicide Efficacy for Control of Fusarium Head Blight on Barley
Effect of Nozzles on Fungicide Efficacy for Control of Fusarium Head Blight on Barley Halley, S. 1 *, Van Ee, G. 2 and Hofman, V. 3 1 Langdon Research Extension Center, North Dakota State University, Langdon,
More informationNorth Central Soybean Research Program. Seedling Diseases: Biology, Management and Education
North Central Soybean Research Program Seedling Diseases: Biology, Management and Education Jason Bond (Project Leader, University of Illinois- Carbondale, Leonor Leandro (Iowa State University), Gary
More informationPredicting the Unpredictable: Disease Factors in Small Grain Production. Juliet M. Marshall. Idaho Falls and Aberdeen R&E Centers
Predicting the Unpredictable: Disease Factors in Small Grain Production Juliet M. Marshall Idaho Falls and Aberdeen R&E Centers Fusarium Head Blight Management History Disease first described in the U.S.
More informationStategic Planning for Mitigation of Fusarium Head Blight in Wheat
Stategic Planning for Mitigation of Fusarium Head Blight in Wheat Anita Brûlé-Babel Department of Plant Science One University. Many Futures. FHB Issue Prior to 1980 FHB epidemics were sporadic Since early
More informationFusarium root rot of soybean occurrence, impact, and relationship with soybean cyst nematode
Proceedings of the Integrated Crop Management Conference Proceedings of the 22nd Annual Integrated Crop Management Conference Dec 1st, 12:00 AM Fusarium root rot of soybean occurrence, impact, and relationship
More informationCo-Chairpersons: Jim Pestka and Paul Schwarz
session 2: Food SAFETY and TOXICOLOGY Co-Chairpersons: Jim Pestka and Paul Schwarz Impact of Bran Properties on Fusarium Mycotoxin Levels in Winter Wheat (Triticum aestivum L.) Kernels H.D.P. Damecharla
More informationBREEDING MAIZE FOR TOLERANCE TO FUSARIUM STALK AND EAR ROT STRESS
BREEDING MAIZE FOR TOLERANCE TO FUSARIUM STALK AND EAR ROT STRESS ABSTRACT Stalk and ear rot is the most harmful maize disease in Romania, and yield losses on the average of 20% (between 1.540%) have been
More informationFusarium thapsinum is the dominant species associated with sorghum stalk rot in Queensland and northern New South Wales
Department of Employment, Economic Development and Innovation Fusarium thapsinum is the dominant species associated with sorghum stalk rot in Queensland and northern New South Wales Lisa Kelly, Malcolm
More informationMycotoxin Testing Solutions
Mycotoxin Testing Solutions Mycotoxin Testing Solutions DON Fumonisin We Stand Behind Our Results AFLATOXIN is considered by many to be the most potent naturally-occurring carcinogen known. It has been
More informationServices and research to promote grain quality management
The Southern African Grain Laboratory NPC Quality is our passion Services and research to promote grain quality management GRAIN SILO INDUSTRY - MINI SYMPOSIUM CONTINUOUS IMPROVEMENT IDEAS, BEST PRACTICE
More informationLast findings on T2/HT2 on malting barley and behaviour from malting barley to malt. Dr Régis Fournier, IFBM
Last findings on T2/HT2 on malting barley and behaviour from malting barley to malt Dr Régis Fournier, IFBM Aim Fusarium contamination occurs in the field: control has to be setup within the field Growth
More informationPS Occurence of Fusarium species isolated from winter wheat and barley grains in Croatia
PS2-1-6118 Occurence of Fusarium species isolated from winter wheat and barley grains in Croatia J. Cosic 1*, D. Jurkovic 1, K. Vrandecic 1, B. Šimic 2 Abstract Fusarium head blight of small grain cereals
More informationPaul Williams USDA-ARS, Mississippi State, MS Evaluation of GEM Germplasm for Resistance to Aflatoxin Accumulation 2007 Results
Paul Williams USDA-ARS, Mississippi State, MS Evaluation of GEM Germplasm for Resistance to Aflatoxin Accumulation 2007 Results Contamination of corn grain with aflatoxin, a toxin produced by the fungus
More informationContamination of winter wheat with Fusarium mycotoxin depending on environment conditions and cultivar
Contamination of winter wheat with Fusarium mycotoxin depending on environment conditions and cultivar Grażyna Podolska, Edyta Boguszewska Department of Cereal Crop Production, Institute of Soil Science
More informationMycotoxin toxicity to animals
Page 1 of 5 Technical Note 06 Mycotoxin toxicity to animals Disclaimer Most inquiries about mycotoxins concern incidents of the poisoning of farm animals, often very expensive breeds. The toxicological
More informationCharacterization and Tracking of Strains of the Potato Blight Pathogen in Canada AgriInnovation Program Industry-led Research and Development Stream
Final Report March 2018 Name of Lead Researcher: Rick D. Peters, AAFC-Charlottetown Research and Development Centre Names of Collaborators and Institutions: CoPI: Larry Kawchuk, AAFC-Lethbridge Research
More informationAn integrated approach to mycotoxin testing in the poultry feed chain. Bankok, March 2010
An integrated approach to mycotoxin testing in the poultry feed chain Bankok, March 2010 OUTLINE Mycotoxins in poultry feed Integrated testing plan Sampling On-site testing Laboratory testing Mycotoxin
More informationOverview of Mycotoxins in India with special reference to Aflatoxins. F Waliyar
Overview of Mycotoxins in India with special reference to Aflatoxins F Waliyar Important Mycotoxins Aflatoxins Ochratoxins Fusarial toxins Fumonisins Zearalenone T2-toxin Deoxynivalenol Nivalenol Mycotoxin
More informationPathology and Mycotoxins
Pathology and Mycotoxins Aflatoxin B 1 and G, Production in Developing Zea mays Kernels from Mixed Inocula of Aspergillus flavus and A. parasiticus 0. H. Calvert, E. B. Lillehoj, W. F. Kwolek, and M. S.
More informationMycocheck Survey 2014
Mycocheck Survey 14 INTRODUCTION Mycotoxins are secondary metabolites produced by moulds that have the capacity to impair animal health and productivity. Contamination of forages and cereals with mycotoxins
More informationOccurrence of mycotoxin producing Fusarium species and other fungi on wheat kernels harvested in selected districts of Kenya
African Crop Science Conference Proceedings, Vol. 6. 335-339 Printed in Uganda. All rights reserved ISSN 123-7X $ 4. 23, African Crop Science Society Occurrence of mycotoxin producing Fusarium species
More informationPATHOGEN BIOLOGY AND GENETICS
PATHOGEN BIOLOGY AND GENETICS SURVEY FOR FUSARIUM GRAMINEARUM 15-ADON, 3-ADON AND NIV CHEMOTYPES IN WINTER WHEAT IN ONTARIO Pragyan Burlakoti and Ljiljana Tamburic-Ilincic Ridgetown Campus, University
More informationReduction of mycotoxins in cereals through an Integrated Crop Management approach
Reduction of mycotoxins in cereals through an Integrated Crop Management approach Dr. Alfons Sagenmüller Slide 1 EUREPGAP Integrated Farm Assurance Conference Amsterdam, The Netherlands November 9 th -
More informationRESULTS OF AGRONOMIC AND WEED SCIENCE RESEARCH CONDUCTED IN SOUTH CENTRAL MONTANA
RESULTS OF AGRONOMIC AND WEED SCIENCE RESEARCH CONDUCTED IN SOUTH CENTRAL MONTANA - 2005 The Annual Report of the Investigations at and Administration of the Southern Agricultural Research Center, Huntley,
More information= 0.002) 117 #!. 12, : = 0.45; P
Background: Psychosocial factors governing the use of postoperative, intravenous patient-controlled analgesia (PCA) have received little attention in spite of the fact that PCA is the most common modality
More informationSeasonal and daily variation in the airborne concentration of Gibberella zeae (Schw.) Petch spores in Manitoba
357 Epidemiology / Épidémiologie Seasonal and daily variation in the airborne concentration of Gibberella zeae (Schw.) Petch spores in Manitoba S. Inch, W.G.D. Fernando, and J. Gilbert Abstract: The aerial
More informationFusarium cob rot management in sweetcorn
Fusarium cob rot management in sweetcorn Andrew Watson NSW Agriculture Project Number: VG02108 VG02108 This report is published by Horticulture Australia Ltd to pass on information concerning horticultural
More informationHT-2 and T-2 in Oats - What we know and what we need to know
HT-2 and T-2 in Oats - What we know and what we need to know Simon Edwards and Samuel Imathiu Harper Adams University College Shropshire, UK sedwards@harper-adams.ac.uk Hans Pettersson Swedish University
More informationBât. E - Bd Armand Duportal - F Toulouse 3
Bt maize: a tool for improving food safety of grains at harvest Regnault-Roger, C.*# 1, Folcher, L. 1, Delos, M. 2, Jarry, M. 3,6, Weissenberger, A. 4, Eychenne, N. 5 1 Université de Pau et des Pays de
More informationP.J. Cotty, Page NO.1 of 8.
P.J. Cotty, Page NO.1 of 8. POSITION AND AFLATOXIN LEVELS OF TOXIN POSITIVE BOLLS ON COTTON PLANTS. P.J. Cotty and L.S. Lee Research Plant Pathologist and Research Chemist USDA, ARS, Southern Regional
More informationFINAL DRAFT EAST AFRICAN STANDARD
FINAL DRAFT EAST AFRICAN STANDARD Milled maize (corn) products Specification EAST AFRICAN COMMUNITY ICS 67.060 EAC 2011 Second Edition 2011 Foreword Development of the East African Standards has been necessitated
More informationIn-vitro determination of kernel resistance to Aspergillus flavus and aflatoxin accumulation in groundnut
Third RUFORUM Biennial Meeting 24-28 September 2012, Entebbe, Uganda Research Application Summary In-vitro determination of kernel resistance to Aspergillus flavus and aflatoxin accumulation in groundnut
More informationProject title: Fusarium wilt of lettuce: management through detection, avoidance and disease resistance
Abstract Project title: Fusarium wilt of lettuce: management through detection, avoidance and disease resistance Principal investigator: Thomas R. Gordon Department of Plant Pathology University of California
More informationM. W. Parsons a and G. P. Munkvold b* Introduction
Doi: 10.1111/j.1365-3059.2011.02590.x Effects of planting date and environmental factors on fusarium ear rot symptoms and fumonisin B 1 accumulation in maize grown in six North American locations M. W.
More informationPRAIRIE RECOMMENDING COMMITTEE FOR OILSEEDS (PRCO) APPENDIX C
PRAIRIE RECOMMENDING COMMITTEE FOR OILSEEDS (PRCO) APPENDIX C Operating Procedures for the Registration of a Flax Cultivar for Production in Western Canada Table of contents C1. Background...2 C2. The
More informationPredicting NuSun Hybrid Oleic Acid Concentration Through Early Sampling. J. F. Miller, D. A. Rehder, and B. A. Vick
Predicting NuSun Hybrid Oleic Acid Concentration Through Early Sampling J. F. Miller, D. A. Rehder, and B. A. Vick USDA-ARS Northern Crop Science Laboratory, PO Box 5677, Fargo, ND 58105 Introduction The
More informationUsing Near-Infrared Spectroscopy to Select for Resistance to FHB Various Applications
Using Near-Infrared Spectroscopy to Select for Resistance to FHB Various Applications Floyd Dowell, Ph.D. Stored Product Insect and Engineering Research Unit USDA ARS Center for Grain and Animal Health
More informationDRAFT EAST AFRICAN STANDARD
DEAS 44:2016 ICS 67.060 DRAFT EAST AFRICAN STANDARD Milled maize (corn) products Specification EAST AFRICAN COMMUNITY EAS 2016 First Edition 2016 Copyright notice This EAC document is copyright-protected
More informationWheat Update September 2017 ARKANSAS WHEAT PERFORMANCE TRIALS AND VARIETY SELECTION. Methods
September 2017 Wheat Update 2017 DR. JASON P. KELLEY Extension Agronomist Wheat and Feed Grains DR. ESTEN MASON Associate Professor Wheat Breeding and Genetics RANDY MILLER Program Associate DAVID MOON
More informationPROJECT PROPOSAL SUGARBEET RESEARCH AND EDUCATION BOARD OF MINNESOTA AND NORTH DAKOTA FY
1 PROJECT PROPOSAL SUGARBEET RESEARCH AND EDUCATION BOARD OF MINNESOTA AND NORTH DAKOTA FY 2018 2019 Project Title: SCREENING OF SUGAR BEET GERMPLASM FOR RESISTANCE TO FUSARIUM YELLOWING DECLINE Project
More informationEvaluation of African-Bred Maize Germplasm Lines for Resistance to Aflatoxin Accumulation
agronomy Article Evaluation of African-Bred Maize Germplasm Lines for Resistance to Aflatoxin Accumulation Robert L. Brown 1, *,, W. Paul Williams 2,, Gary L. Windham 2,, Abebe Menkir 3, and Zhi-Yuan Chen
More informationManaging transplant size and advancing field maturity of fresh tomatoes and peppers
Managing transplant size and advancing field maturity of fresh tomatoes and peppers J.W. Zandstra R.C. Squire G.J. Watt CORD IV Project # 9022 Final Report 15 November, 2007 Prepared for: Fresh Vegetable
More informationThermo-Therapy and Use of Biofungicides and Fungicides for Management of Internal Discoloration of Horseradish Roots
Thermo-Therapy and Use of Biofungicides and Fungicides for Management of Internal Discoloration of Horseradish Roots - 2006 1 M. Babadoost, 1 A. Eranthodi, 1 A. Jurgens, 1 K. Hippard, and 2 E. Wahle 1
More informationGenetic Diversity and Fumonisin Analyses of Fusarium species in the Philippines
Genetic Diversity and Fumonisin Analyses of Fusarium species in the Philippines ASIAHORC Symposium July 18-20, 2009 Nagoya, Japan Christian Joseph R. CUMAGUN Crop Protection Cluster College of Agriculture
More informationThe Efficacy of Spraying Organic Fungicides to Control Fusarium Head Blight Infection in Spring Wheat
The Efficacy of Spraying Organic Fungicides to Control Fusarium Head Blight Infection in Spring Wheat Dr. Heather Darby, UVM Extension Agronomist Erica Cummings, UVM Extension Crops and Soils Coordinator
More informationCrop Staging guide FungiCideS
Crop Staging Guide Fungicides Take a stand. Stop disease before it starts. Once yield is lost to disease, it s gone forever. There s no cure. The best way to prevent this yield loss is to stop disease
More informationM. S. ZUBER A. J. KEASTER N. G. WEIR C. F. STARK P. J. LOESCH, JR. January,1970
1 1 M. S. ZUBER A. J. KEASTER N. G. WER C. F. STARK P. J. LOESCH, JR. January,1970 THE AUTHORS M. S. Zuber is research agronomist, Crops Research Division, Agricultural Research Service, U. S. Department
More informationFusarium Head Scab Management in Alabama Wheat
PLANT PATHOLOGY SERIES TIMELY INFORMATION Agriculture & Natural Resources EXTENSION PLANT PATHOLOGY, 961 S. DONAHUE DRIVE, AUBURN UNIVERSITY, AL 36849-5624 April 15, 2014 PP-747 Fusarium Head Scab Management
More informationAflatoxin: What is it, and why worry? Doug Jardine Professor Kansas State University
Aflatoxin: What is it, and why worry? Doug Jardine Professor Kansas State University Aflatoxin facts Aflatoxins are metabolic products produced by the fungus Aspergillus flavus and several other species
More informationPreliminary report of Dehulling effect on the occurrence and distribution of Aspergillus flavus in maize grains stored in Mubi market
Available online at www.pelagiaresearchlibrary.com Advances in Applied Science Research, 2011, 2 (5):612-616 ISS: 0976-8610 CODE (USA): AASRFC Preliminary report of Dehulling effect on the occurrence and
More informationGenetics of resistance to ear diseases and mycotoxin accumulation in the pathosystems maize/fusarium and wheat/fusarium
Aus der Landessaatzuchtanstalt der Universität Hohenheim Apl. Prof. Dr. T. Miedaner Genetics of resistance to ear diseases and mycotoxin accumulation in the pathosystems maize/fusarium and wheat/fusarium
More informationMYCOTOXINS IN PLANT DISEASE
MYCOTOXINS IN PLANT DISEASE Mycotoxins in Plant Disease Under the aegis of COST Action 835 'Agricu1turally Important Toxigenic Fungi 1998-2003', EU project (QLK 1-CT-1998-01380), and ISPP 'Fusarium Committee'
More informationPossible climate change impact on occurrence of Aspergillus flavus on spelt wheat in Serbia
Possible climate change impact on occurrence of Aspergillus flavus on spelt wheat in Serbia Jelena KRULJ * * University of Novi Sad, Institute of Food Technology, 21000 Novi Sad, Bulevar cara Lazara 1,
More informationQ U A L I T Y I N N O V A T I O N S E R V I C E
Q U A L I T Y I N N O V A T I O N S E R V I C E Fusarium graminearum: not the only Fusarium species that should be of concern for Canada's grain industry Tom Gräfenhan Microbiology Program, Grain Research
More informationThird International Scientific Symposium "Agrosym Jahorina 2012"
10.7251/AGSY1203092R UDK 633.15-152.6 MICROELEMENTS CONCENTRATIONS IN MAIZE INBRED LINES GROWN ON ACID SOIL Mirta RASTIJA 1*, Vlado KOVACEVIC 1, Domagoj SIMIC 2, Tomislav BRKANIC 1 1 University of J. J.
More informationGrain Sorghum as a Feedstuff For Livestock
Grain Sorghum as a Feedstuff For Livestock What can I do right now to make my farm more viable? well, Anything and everything that contributes to lower cost of gain!!! Improved genetics Improved health
More informationGENOTYPIC AND PHENOTYPIC CHARACTERIZATION OF RESPONSE TO AFLATOXIN AND SECONDARY TRAITS IN MAIZE. A Dissertation MELANIE LOVE EDWARDS
GENOTYPIC AND PHENOTYPIC CHARACTERIZATION OF RESPONSE TO AFLATOXIN AND SECONDARY TRAITS IN MAIZE A Dissertation by MELANIE LOVE EDWARDS Submitted to the Office of Graduate Studies of Texas A&M University
More informationTask Force High Oil Corn Composition. Dirk E. Maier & Jenni L. Briggs, Agricultural & Biological Engineering $.30 $.53 $.25 $45 $.315 $.
GRAIN QUALITY Task Force High Oil orn omposition Dirk E. Maier & Jenni L. Briggs, Agricultural & Biological Engineering Fact Sheet #33 November 19, 1997 We have received numerous inquiries about oil levels
More informationTHE INFLUENCE OF THE NUMBER OF FUNGICIDE TREATMENTS UPON THE QUANTITY AND QUALITY OF WINTER WHEAT YIELD IN CLIMATIC CONDITIONS OF ARDS TURDA
NARDI FUNDULEA, ROMANIA ROMANIAN AGRICULTURAL RESEARCH, NO. 35, 2018 www.incda-fundulea.ro Print ISSN 1222 4227; Online ISSN 2067 5720 THE INFLUENCE OF THE NUMBER OF FUNGICIDE TREATMENTS UPON THE QUANTITY
More informationFactors Influencing Deoxynivalenol Accumulation in Small Grain Cereals
Toxins 2012, 4, 1157-1180; doi:10.3390/toxins4111157 Review OPEN ACCESS toxins ISSN 2072-6651 www.mdpi.com/journal/toxins Factors Influencing Deoxynivalenol Accumulation in Small Grain Cereals Stephen
More informationMycotoxins Overview and Sampling to Testing
Mycotoxins Overview and Sampling to Testing Webinar August 3, 2012 John L. Richard, Ph.D. Consultant to Romer Labs, Inc. Early History of Mycotoxins Alsberg and Black, 1913 Described toxicity of mold metabolites
More informationDETERMINATION OF TOXIGENIC FUNGI AND THEIR ASSOCIATED MYCOTOXINS IN MAIZE FROM MERU COUNTY JOHN NDERITU GATHOGO SUPERVISOR: PROF: R.
DETERMINATION OF TOXIGENIC FUNGI AND THEIR ASSOCIATED MYCOTOXINS IN MAIZE FROM MERU COUNTY NAME: REG NO: JOHN NDERITU GATHOGO A22/1803/2010 SUPERVISOR: PROF: R.D NARLA INTRODUCTION Maize belongs to gramineae
More informationThe Influence of Fungicide Treatment on the Production and the Quality of Several Autumn Wheat Varieties
The Influence of Fungicide Treatment on the Production and the Quality of Several Autumn Wheat Varieties Loredana SUCIU 1,2, Raluca MICLEA 1, Rozalia KADAR 2, Carmen PUIA 1* 1 University of Agricultural
More information