LAB Catalase in Liver HONORS BIOLOGY, NNHS
|
|
|
- Trevor Austin
- 6 years ago
- Views:
Transcription
1 Name Date Block LAB Catalase in Liver HONORS BIOLOGY, NNHS OBJECTIVES: 1. To observe the effect of catalase on the chemical breakdown of hydrogen peroxide. 2. To observe the effects of temperature and ph on catalytic activity. BACKGROUND INFORMATION: Hydrogen peroxide is a highly reactive chemical, often used for bleaching and for cleaning minor wounds. It is also formed continually as a by-product of chemical reactions in living cells, but is poisonous, and must be immediately removed or broken down by the cells. In living cells, hydrogen peroxide is converted into two harmless substances, oxygen and water. For nearly every chemical reaction in organisms, enzymes are present which greatly speed up the rate of the reaction. In this experiment, you will see the effect of an enzyme called catalase, which speeds up the breakdown of hydrogen peroxide. Catalase can be found in the liver of chickens and cows. The breakdown of peroxide can be detected by bubbles of oxygen that are released and by using the glowing splint test for oxygen. You will have an opportunity to investigate the effect of temperature, and ph on catalase activity. MATERIALS: 3% hydrogen peroxide (H 2 O 2 ) acid (HCl, 0.1M) matches liver base (NaOH, 0.1M) test tube candle test tube holder forceps wood splints hot water bath PROCEDURE - For A Glowing Splint Test: 1. Light the end of a wood splint with a match or lit candle. 2. Gently blow out the flame on the end of the wood splint so that the wood is glowing orange. 3. Place the glowing splint into the test tube to test for the presence of oxygen gas. If the wood split re-lights, then there is oxygen present. If the wood split does not re-light by goes out, then oxygen is not present. N. Berg Page 1 of 5
2 PROCEDURE: Part A: Temperature Test 1. Pour hydrogen peroxide into a test tube to a depth of about one centimeter (the width of your pinky finger). 2. Liver is an organic material, which contains the enzyme catalase. Pick up a small piece of fresh liver and drop it into the tube. Place your thumb over the mouth of the tube and shake gently. Observe the reaction and test for oxygen with a glowing splint. Record your results. Clean your test tube. 3. Place a small piece of boiled liver of the same size you used before in a test tube. Repeat steps 1 & 2. Record your results. Clean your test tube. Part B: Acid Test 4. Pour hydrochloric acid (HCl) solution into a test tube to a depth of about 1-cm. Pour about 1- cm of hydrogen peroxide into the test tube. Use a glowing splint to test for the catalytic activity of the acid. Record your results. Clean your test tube. 5. Place a small piece of fresh liver in a test tube. Carefully pour in just enough hydrochloric acid solution to cover the liver. Let the liver soak in acid for three minutes. After three minutes, pour off the acid into the proper waste beaker and rinse the liver with water three times. 6. Pour hydrogen peroxide into the test tube with the liver that was soaked in acid to a depth of about one centimeter. Place your thumb over the mouth of the tube and shake gently. Observe the reaction and test for oxygen with a glowing splint. Record your results. Clean your test tube. Part C: Base Test 7. Pour sodium hydroxide (NaOH) solution into a test tube to a depth of about 1-cm. Pour about 1-cm of hydrogen peroxide into the test tube. Use a glowing splint to test for the catalytic activity of the base. Record your results. Clean your test tube. 8. Place a small piece of fresh liver in a test tube. Carefully pour in just enough sodium hydroxide solution to cover the liver. Let the liver soak in base for three minutes. After three minutes, pour off the sodium hydroxide solution into the proper waste container and rinse the liver with water three times. 9. Pour hydrogen peroxide into the test tube with the liver that was soaked in base to a depth of about one centimeter. Place your thumb over the mouth of the tube and shake gently. Observe the reaction and test for oxygen with a glowing splint. Record your results. Clean your test tube. LAB: Catalase in Liver Page 2 of 5
3 Wrap Up 10. While you remember the reactions, fill in the fourth column of your data chart, estimating the relative activity of each catalyst on a scale of 0-10 (0 = no catalytic activity; 10 = very strong catalytic activity). 11. Carefully clean all the glassware you have used and return lab tray. DATA: Record your observations in the chart below. Record the amount of bubbles released and whether or not the glowing splint indicated the presence of oxygen. In the column on the right, estimate of the different rates of catalytic activity, using a scale of 0 to 10 (0 = no detectable activity; 10 = extremely high activity). Substance placed in the H 2 O 2 Catalytic Activity of Various Substances Catalytic Activity Measured By Amount of Bubbling: Lots Some Little None Glowing Splint Test: Lights! Nothing Amount of Activity Rated by # (0-10) Additional DETAILED Observations Liver (at room temperature) Liver (boiled) Hydrochloric Acid (ph= 2) hydrochloric acid Sodium Hydroxide (ph= 12) sodium hydroxide LAB: Catalase in Liver Page 3 of 5
4 CLASS DATA: Substance placed in the H 2 O 2 Catalytic Activity Measured By Amount of Bubbling: Glowing Splint Test: Amount of Activity Rated by # (0-10) NOTES/COMMENTS Liver (at room temperature) Liver (boiled) Hydrochloric Acid (ph= 2) hydrochloric acid Sodium Hydroxide (ph= 12) sodium hydroxide ADDITIONAL NOTES FROM DISCUSSION: LAB: Catalase in Liver Page 4 of 5
5 ANALYSIS QUESTIONS: Please TYPE the answers to this lab report. Staple to this lab. Use complete sentences. A. THE CHEMICAL REACTION: a. What was the enzyme in this lab? b. What was the substrate? c. What were the products? B. GLOWING SPLINT TEST: a. What did the glowing splint test show? b. How is it related to the enzyme s activity? C. PREDICTING & REASONING: Predict what effect grinding a piece of liver at room temperature has on the catalytic activity of catalase. Explain your prediction. D. IDENTIFYING ERROR: Identify one source of error and explain how it might affect the outcome of the results. E. CLAIM: It is important that your body carefully control the temperature and ph of its tissues. EVIDENCE & REASONING: Refer to two specific pieces of evidence that support the claim above. Explain why is it important that your body carefully controls the temperature and ph of its tissues. In your explanation you must use the following terms correctly: enzyme, active site, substrate, product, primary structure, R-groups, denature, and reaction rate. LAB: Catalase in Liver Page 5 of 5
Catalase Lab - A Bio ENZYME ACTIVITY Investigation Created by Gen Nelson, modified by Dr G
Catalase Lab - A Bio ENZYME ACTIVITY Investigation Created by Gen Nelson, modified by Dr G INTRODUCTION Hydrogen peroxide (H 2O 2) is a poisonous byproduct of metabolism that can damage cells if it is
Problem: What would happen to enzyme activity if enzymes are placed outside their normal conditions? Hypothesis:
Name: Date: Period: Honors Biology: Enzyme Lab Background information What would happen to your cells if they made a poisonous chemical? You might think that they would die. In fact, your cells are always
EXERCISE 5. Enzymes H amylase + starch + amylase-starch complex maltose+ amylase.
EXERCISE 5 Enzymes LEARNING OBJECTIVES Demonstrate enzyme activity by the hydrolysis of starch by amylase. Determine the effect of different temperatures on the rate of starch hydrolysis. Determine the
Investigation: Enzymes
Investigation: Enzymes INTRODUCTION: What would happen to your cells if they made a poisonous chemical? You might think that they would die. In fact, your cells are always making poisonous chemicals. They
Catalytic Activity of Enzymes
Catalytic Activity of Enzymes Introduction Enzymes are biological molecules that catalyze (speed up) chemical reactions. You could call enzymes the Builders and Do-ers in the cell; without them, life could
Enzyme Action: Testing Catalase Activity
Enzyme Action: Testing Catalase Activity LabQuest 6A Many organisms can decompose hydrogen peroxide (H 2 O 2 ) enzymatically. Enzymes are globular proteins, responsible for most of the chemical activities
Name: There are two things that will determine which particles will pass through and which will not:
18 Diffusion and Osmosis in Living Systems Name: Problem: How do substances move into and out of cells? Introduction: In order for cells to carry on their life processes, they must take in materials and
MiSP ENZYME ACTION Teacher Guide, L1 - L3. Introduction
MiSP ENZYME ACTION Teacher Guide, L1 - L3 Introduction The subject of this unit, enzymes, is typically a high school topic and is studied in depth in Advanced Placement Biology. Even so, it can be successfully
Name: Date: AP Biology LAB : FACTORS INFLUENCING ENZYME ACTIVITY
LAB : FACTORS INFLUENCING ENZYME ACTIVITY Background Enzymes are biological catalysts capable of speeding up chemical reactions by lowering activation energy. One benefit of enzyme catalysts is that the
The Effect of Hydrogen Peroxide Concentration (substrate) on the Activity of the Enzyme Catalase
The Effect of Hydrogen Peroxide Concentration (substrate) on the Activity of the Enzyme Catalase Exercise adapted from: Allot, A. & Mindorff, D. (2007). Biology Course Companion. Oxford: Oxford University
3. Which of the following cannot increase metabolic rate? A. Reading B. Sleeping C. Talking D. Jogging
Topic 4: Enzymes and metabolism 1. An is only required in a small amount because A. its shape is specific. B. it speeds up both forward and backward reactions. C. it is made of proteins. D. it is not consumed
Enzymes: What s in your spit? Teacher Version
Enzymes: What s in your spit? Teacher Version In this lab students will investigate a few of the different enzymes from our body. You will learn how these enzymes work and how their activity is dependent
ENZYME ACTION: TESTING CATALASE ACTIVITY
Name Date Period ENZYME ACTION: TESTING CATALASE ACTIVITY Many organisms can decompose hydrogen peroxide (H 2 O 2 ) enzymatically. Enzymes are globular proteins, responsible for most of the chemical activities
Evaluation copy. Enzyme Action: Testing Catalase Activity. Computer
Enzyme Action: Testing Catalase Activity Computer 6A Many organisms can decompose hydrogen peroxide (H 2 O 2 ) enzymatically. Enzymes are globular proteins, responsible for most of the chemical activities
Name: Date Block Selective Permeability
LAB Name: Date Block Selective Permeability OBJECTIVES: Observe the selective permeability of an artificial membrane. Observe diffusion of substances across an artificial membrane. Devise a model for the
The Hydrogen Peroxide Breakdown
Biology The Hydrogen Peroxide Breakdown Examining Factors That Affect the Reaction Rate of Enzymes MATERIALS AND RESOURCES EACH GROUP aprons 2 beakers, 50 ml calculator, graphing forceps goggles graduated
ENZYME CONCENTRATIONS AND ENZYME ACTIVITY: PLANNING SHEET
Activity 2.11 Student Sheet ENZYME CONCENTRATIONS AND ENZYME ACTIVITY: PLANNING SHEET To investigate how enzyme concentration can affect the initial rate of reaction. Wear eye protection, lab coats and
Challenge Finding which plants have an enzyme called catalase That breaks hydrogen peroxide into water and oxygen.
Challenge Finding which plants have an enzyme called catalase That breaks hydrogen peroxide into water and oxygen. General Description Visitors work with 3% hydrogen peroxide and several different fruits
Digestive Enzyme Lab
Digestive Enzyme Lab Objectives 1. To describe the function of enzymes 2. To define: reactants, products, activation energy 3. To describe the enzymatic digestion of carbohydrates by salivary amylase 4.
In this lab, you will determine, through observation, which protease is secreted into the stomach, and which is secreted into the small intestine.
Lab 2: Protein and Fat Digestion LABORATORY OBJECTIVES To investigate the effect of ph and digestive enzymes on the digestion of proteins To investigate the action of lipase on the breakdown of fats INTRODUCTION:
Gas Tests. Distinguishing Chemical Property acts as the fuel for combustion, allowing things to burn more vigorously in its presence
Gas Tests Date: As we have learned, air is made up of a mixture of many gases, including nitrogen, oxygen, carbon dioxide and hydrogen. Most of these gases are colourless, odourless, and, making them difficult
Source 1 Evaluation. Source (using Harvard reference style)
1 Evaluation (using Harvard reference style) Type of Overview of Secondary Relevance and Reliability of the Morton, D. and Perry, J.B. (2011) Laboratory manual for human biology. Available at: https://books.google.com.au/books?id=aenovttzlpcc&pg=pt78&dq=the
AP BIOLOGY Enzyme Catalysis
AP BIOLOGY Enzyme Catalysis Introduction In general, enzymes are proteins produced by living cells; they act as catalysts in biochemical reactions. A catalyst affects the rate of a chemical reaction. One
Evaluation copy 17B. Enzyme Action: Testing Catalase Activity. Computer
Enzyme Action: Testing Catalase Activity Computer 17B Many organisms can decompose hydrogen peroxide (H 2 O 2 ) enzymatically. Enzymes are globular proteins, responsible for most of the chemical activities
BIOLOGY 1101 LAB 1: OSMOSIS & DIFFUSION. READING: Please read pages & in your text prior to lab.
BIOLOGY 1101 LAB 1: OSMOSIS & DIFFUSION READING: Please read pages 27-31 & 83-86 in your text prior to lab. INTRODUCTION: All living things depend on water. A water molecule is made up of an oxygen atom
Name Group Members. Table 1 Observation (include details of what you observe)
Name Group Members Macromolecules, Part 1 - PROTEINS There are four classes of macromolecules that are important to the function of all living things. These include carbohydrates, lipids, proteins and
Enzyme Action: Testing Catalase Activity
Enzyme Action: Testing Catalase Activity Pennsylvania Science Standards: S11.A.1.1.4 S11.A.1.3.1 S11.A.2.2.2.1 S11.A.2.2.2.2 Keystone Eligible Content Bio.B.4.1.1, Bio.B.4.1.2, and Bio.B.4.2.5 Introduction
Lab: Enzymes and the factors that affect their function
Name Date Hour Lab: Enzymes and the factors that affect their function INTRODUCTION: What would happen to your cells if they made a poisonous chemical? You might think that they would die. If fact, your
Topic 4: Enzymes and metabolism
Topic 4: Enzymes and metabolism 1. An is a living molecule produced by glands to digest food in the alimentary canal. living molecule produced by cells to synthesise complex molecules from simpler ones.
Terminology-Amino Acids
Enzymes 1 2 Terminology-Amino Acids Primary Structure: is a polypeptide (large number of aminoacid residues bonded together in a chain) chain of amino acids linked with peptide bonds. Secondary Structure-
(LM pages 91 98) Time Estimate for Entire Lab: 2.5 to 3.0 hours. Special Requirements
Laboratory 7 Chemical Aspects of Digestion (LM pages 91 98) Time Estimate for Entire Lab: 2.5 to 3.0 hours Special Requirements Incubation. Students should start these sections at the beginning of the
Amylase: a sample enzyme
Amylase: a sample enzyme Objectives: After completion of this laboratory exercise you will be able to: 1. Explain the importance of enzymes in biology. 2. Explain the basic properties of an enzyme as a
Enzymes. Cell Biology. Monday, November 02, 2015 Mrs Wrightson
Enzymes Cell Biology 1 Enzymes 2 Recap Enzymes are specific: They only act with one substrate. Watch Me Type of Reaction Substrate Enzyme Product Degradation Starch Amylase Maltose Degradation Protein
Name: Enzymes. Class: Date: 101 minutes. Time: 100 marks. Marks: Level 1,2 and 3. Increasing demand. Comments:
Enzymes Name: Class: Date: Time: 0 minutes Marks: 00 marks Comments: Level,2 and 3. Increasing demand Q. Catalase is an enzyme. Catalase controls the following reaction: hydrogen peroxide water + oxygen
Rate of Decomposition of Hydrogen Peroxide as a Function of Catalase. Concentration
Rate of Decomposition of Hydrogen Peroxide as a Function of Catalase Concentration Performed: Herrin High School September 11, 2017 Mr. Johns INTRODUCTION Purpose: To determine how the concentration of
TRATION: ANALYSIS OF SODIUM HYDROXIDE
Experiment 8 Name: 22 Ti TRATION: ANALYSIS OF SODIUM HYDROXIDE In this experiment, you will learn the concept and technique of titration. You will use a chemical standard (potassium hydrogen phthalate)
ENZYME ACTIVITY. Introduction
ENZYME ACTIVITY This activity is an alternative to the titration proposed for Enzyme Catalysis (AP Bio Lab #2, Biology Lab Manual). There are numerous alternative lab activities that measure the rate of
Identification of Organic Compounds Lab
Identification of Organic Compounds Lab Introduction All organic compounds contain the element carbon (C). Organic compounds usually also contain oxygen (O) or hydrogen (H) or both. They may also contain
4. 10/09/14 Ch. 5: Populations /22/14 Ch. 2: Chemistry of Life 55
Table of Contents # Date Title Page # 1. 1 2. 09/02/14 Ch. 1: The Science of Biology 09/16/14 Ch. 4: Ecosystems and Communities 17 3. 09/23/14 Ch. 3: The Biosphere 26 4. 10/09/14 Ch. 5: Populations 45
LAB: DIFFUSION ACROSS A SELECTIVELY PERMEABLE MEMBRANE
LAB: DIFFUSION ACROSS A SELECTIVELY PERMEABLE MEMBRANE NAME: PERIOD: DATE: Building Background Knowledge: 1) SELECTIVELY PERMEABLE MEMBRANE: Every cell is surrounded by a selectively permeable membrane
Enzymes: What s in your spit? Student Version
Enzymes: What s in your spit? Student Version In this lab students will investigate a few of the different enzymes from our body. You will learn how these enzymes work and how their activity is dependent
Lab 6: Cellular Respiration
Lab 6: Cellular Respiration Metabolism is the sum of all chemical reactions in a living organism. These reactions can be catabolic or anabolic. Anabolic reactions use up energy to actually build complex
Properties of Alcohols and Phenols Experiment #3
Properties of Alcohols and Phenols Experiment #3 Objectives: To observe the solubility of alcohols relative to their chemical structure, to perform chemical tests to distinguish primary, secondary and
Carbohydrates Chemical Composition and Identification
Carbohydrates Chemical Composition and Identification Introduction: Today, scientists use a combination of biology and chemistry for their understanding of life and life processes. Thus, an understanding
Enzyme Action: Testing Catalase Activity
Enzyme Action: Testing Catalase Activity Many organisms can decompose hydrogen peroxide (H 2 O 2 ) enzymatically. Enzymes are globular proteins, responsible for most of the chemical activities of living
LAB Potato Cores Honors Biology, Newton North High
Name Date Block LAB Potato Cores Honors Biology, Newton North High BACKGROUND: Osmosis is a type of passive transport. No input of energy is needed in order for water to pass through a selectively permeable
fossum/files/2012/01/10 Enzymes.pdf
http://www.laney.edu/wp/cheli fossum/files/2012/01/10 Enzymes.pdf Enzyme Catalysis Enzymes are proteins that act as catalysts for biological reactions. Enzymes, like all catalysts, speed up reactions without
How do abiotic or biotic factors influence the rates of enzymatic reactions?
Big Idea 4 Interactions investigation 13 ENZYME ACTIVITY* How do abiotic or biotic factors influence the rates of enzymatic reactions? BACKGROUND Enzymes are the catalysts of biological systems. They speed
Cell Diffusion & Permeability: See-Through Eggs Teacher Version
Cell Diffusion & Permeability: See-Through Eggs Teacher Version In this lab, students will learn about the permeability of the cell membrane. By studying the ability of a shell-less egg to absorb various
Enzymes & Experimental Design
Lab 4- Bio 201 Name: Enzymes & Experimental Design OBJECTIVES: To continue to practice to apply hypothesis testing. To continue to practice experimental design. To gain a better understanding of enzymes
GCSE. Biology Practical Manual. Unit 3: Practical Skills CCEA GCSE TEACHER GUIDANCE
GCSE CCEA GCSE TEACHER GUIDANCE Biology Practical Manual Unit 3: Practical Skills 1.4 Investigate the effect of temperature on the action of an enzyme For first teaching from September 2017 Practical
LAB: DIFFUSION ACROSS A SELECTIVELY PERMEABLE MEMBRANE
LAB: DIFFUSION ACROSS A SELECTIVELY PERMEABLE MEMBRANE NAME: PERIOD: DATE: Building Background Knowledge: 1) SELECTIVELY PERMEABLE MEMBRANE: Every cell is surrounded by a selectively permeable membrane
Testing the capacity of biological buffer systems to resist changes in ph By P. H. Meter
Testing the capacity of biological buffer systems to resist changes in ph By P. H. Meter Personal Engagement and Exploration Research Question: How do plant and animal tissues respond to changes in ph?
Investigation 13: Enzyme Activity Notes From the teacher
Day 1: Investigation 13: Enzyme Activity Notes From the teacher Before class: Read Entire Lab and Complete Pre Lab. Pre-Lab: 1. What is the difference between catalytic and anabolic enzymes? 2. Describe
MONDAY Review ( SL 2.5)
Enzymes 8.1 MONDAY Review ( SL 2.5) -Active site to which specific substrate binds -Enzymes are catalysts: bring about biochemical reactions! -Optimal environment: affected by ph, temp, and substrate concentration
Enzymes Adapted from Air All Around: Oxygen Investigation
Enzymes Adapted from Air All Around: Oxygen Investigation Author: Doris Pun & Brittland DeKorver Institute for Chemical Education and Nanoscale Science and Engineering Center University of Wisconsin-Madison
Biology: 4. The Digestive System
Biology: 4. The Digestive System Please remember to photocopy 4 pages onto one sheet by going A3 A4 and using back to back on the photocopier OB6 OB7 OB8 Syllabus Identify and locate the major parts of
Cell Diffusion & Permeability: See-Through Eggs Student Advanced Version
Cell Diffusion & Permeability: See-Through Eggs Student Advanced Version In this lab, students will learn about the permeability of the cell membrane. By studying the ability of a shell-less egg to absorb
Standards: Next Generation Science Standards ( )
Discovering Enzymes Author(s): Pascale Chenevier and Gil Toombes Date Created: 2000 Subject: Chemistry Grade Level: Middle & High School Standards: Next Generation Science Standards ( www.nextgenscience.org
The Energy in Food. Energy (J/g) = (final temperature start temperature) x mass of water (g) x 4.2 (J per o C) Mass of food burned (g)
The Energy in Food NAME DATE Food supplies energy for all animals without it we could not live. The quantity of energy stored in food is of great interest to humans. The energy your body needs for running,
250-mL beakers. iodine solution metric ruler. 10-mL graduated cylinders pipettes. (Read the Procedure first to answer the Questions)
Detecting Diffusion Introduction A cell membrane is a selectively permeable barrier. Some particles can pass through the cell membrane while other particles are held back. Solutes that can move across
09 Enzymes. December 04, Chapter 9 Enzymes. Mr. C Biology 1
Chapter 9 Enzymes Mr. C Biology 1 Chapter 9 Enzymes Metabolism is the sum of all chemical reactions in the body. Your metabolism is controlled by enzymes. Enzymes are proteins made in the ribosome from
9. Determine the mass of the fat you removed from the milk and record in the table. Calculation:
Chemistry 100 Instructor s Initials Name: Experiment 14: Biochemistry Analysis of milk for the lipids, carbohydrates, and proteins. A. Determining the % Fat in Whole Milk 1. Weigh a clean, dry, empty 50
SALIVA TEST Introduction
SALIVA TEST Introduction This is a practical lesson using saliva to learn digestive enzyme activity. We can check the existence of reducing sugars clearly by Benedict s reaction after salivary enzyme decomposes
Potions and Poisons B
Potions and Poisons B Science Olympiad North Regional Tournament at the University of Florida Rank: Points: Name(s): Team Name: School Name: Team Number: This exam consists of 4 sections, (1) multiple
Progressive Science Initiative. Click to go to website:
Slide 1 / 28 New Jersey Center for Teaching and Learning Progressive Science Initiative This material is made freely available at www.njctl.org and is intended for the non-commercial use of students and
Properties of Alcohols and Phenols Experiment #3
Properties of Alcohols and Phenols Experiment #3 bjectives: (A) To observe the solubility of alcohols relative to their chemical structure and (B) chemical tests will be performed to distinguish primary,
For example, monosaccharides such as glucose are polar and soluble in water, whereas lipids are nonpolar and insoluble in water.
Biology 4A Laboratory Biologically Important Molecules Objectives Perform tests to detect the presence of carbohydrates, lipids, proteins, and nucleic acids Recognize the importance of a control in a biochemical
Lab 4: Enzymes ANSWER KEY. 1. How could you test to see if an enzyme was completely saturated during an experiment? (2 pts)
Lab 4: Enzymes ANSWER KEY Pre-Lab Questions 1. How could you test to see if an enzyme was completely saturated during an experiment? (2 pts) To test this, you d simply see if the reaction was still taking
Name: Per. Date: / 71 points MACROMOLECULE LAB: Testing for the Presence of Macromolecules
Name: Per. Date: / 71 points MACROMOLECULE LAB: Testing for the Presence of Macromolecules Introduction: There are four broad classes of macromolecules that can be found in living systems. Each type of
Enzymes. Chapter Enzymes and catalysts. Vital mistake. What is an enzyme?
Chapter 3 Enzymes Vital mistake We may not be able to see them, but enzymes are absolutely crucial to the lives of ourselves and all other living organisms. The Quarter Horse (Figure 3.1) is a breed of
Analysis of Polyphenoloxidase Enzyme Activity from Potato Extract Biochemistry Lab I (CHEM 4401)
Analysis of Polyphenoloxidase Enzyme Activity from Potato Extract Biochemistry Lab I (CHEM 4401) Background Enzymes are protein molecules (primarily) that serve as biological catalysts. They are responsible
6 The chemistry of living organisms
Living organisms are composed of about 22 different chemical elements. These are combined to form a great variety of compounds. Six major elements make up almost 99% of the mass of the human body, as shown
Determining the Molecular Mass of an Unknown Acid by Titration
Determining the Molecular Mass of an Unknown Acid by Titration Objectives: To perform an analytical titration. To standardize a basic solution. To determine the equivalent mass of an unknown acid. Background:
How do abiotic or biotic factors influence the rates of enzymatic reactions?
Investigation 13 ENZYME ACTIVITY* How do abiotic or biotic factors influence the rates of enzymatic reactions? BACKGROUND Enzymes are the catalysts of biological systems. They speed up chemical reactions
Classwork #10 - Enzymes Key Vocabulary protein enzyme catalyst reactant substrate active site product
Biology 2017-2018 Noble efforts change lives. Name: Excellence. Tenacity. Community. Reflection. Classwork #10 - Enzymes Key Vocabulary protein enzyme catalyst reactant substrate active site product Pre-Reading
AP Biology Macromolecules
AP Biology Macromolecules Introduction: There are four broad classes macromolecules that can be found in living systems. Each type macromolecule has a characteristic structure and function in living organisms.
DIFFUSON AND OSMOSIS INTRODUCTION diffusion concentration gradient. net osmosis water potential active transport
DIFFUSON AND OSMOSIS NAME DATE INTRODUCTION The life of a cell is dependent on efficiently moving material into and out of the cell across the cell membrane. Raw materials such as oxygen and sugars needed
Properties of an Enzyme: Wheat Germ Acid Phosphatase Experiment #10
Properties of an Enzyme: Wheat Germ Acid Phosphatase Experiment #10 Objective To show the catalysis of a chemical reaction by an active enzyme and to observe the effects of temperature, killing the enzyme
2. Now that you have made your hypothesis, you will begin. must perform at least 3 trials. Pleasee complete
Water Olympics Lab Pre-Lab Questions: 1. What does it mean to be a polar molecule? 2. Draw the water molecule showing its polarity. 3. List and explain the different properties of water. Part 1: Drops
Enzymes - Exercise 3 - Rockville
Enzymes - Exercise 3 - Rockville Objectives -Understand the function of an enzyme. -Know what the substrate, enzyme, and the product of the reaction for this lab. -Understand how at various environments
Name... Class... Date...
Required practical 4: Food tests Aiming for 8 Specification references: Required practical 4: Use qualitative reagents to test for a range of carbohydrates, lipids, and proteins. To include: Benedict s
12BL Experiment 2: Extraction & Saponification of Trimyristin from Nutmeg
12BL Experiment 2: Extraction & Saponification of Trimyristin from Nutmeg Safety: Proper lab goggles/glasses must be worn (even over prescription glasses). Heating of organic solvents releases irritating
How Do Antacids Work?
How Do Antacids Work? Materials Needed: Bottle of antacid (the tablets should be broken into smaller pieces) A Mortar and pestle Dropper bottle with fake stomach juice = (0.01M HCl, or use vinegar) Round
TRATION: ANALYSIS OF SODIUM HYDROXIDE
Experiment 10 Name: 22 Ti TRATION: ANALYSIS OF SODIUM HYDROXIDE In this experiment, you will learn the concept and technique of titration. You will use a standard (potassium hydrogen phthalate) to determine
UNIVERSITY OF CAMBRIDGE INTERNATIONAL EXAMINATIONS International General Certificate of Secondary Education CO-ORDINATED SCIENCES
Centre Number Candidate Number Name UNIVERSITY OF CAMBRIDGE INTERNATIONAL EXAMINATIONS International General Certificate of Secondary Education CO-ORDINATED SCIENCES 0654/05 Paper 5 Practical Test October/November
THE COMPOSITION OF URINE
THE COMPOSITION OF URINE STUDENT BOOK Chapter 6, page 192 TOOLBOX Page 42 LAB 61 OBSERVATION Goal Determine which of four synthetic urine samples most closely resembles natural urine. Observation criteria
SOTM LAB: B1 11/23/99
SOTM LAB: B1 11/23/99 I. TEACHER NOTES & GUIDELINES TITLE OF LAB: An Enzyme Catalyzed Reaction DEVELOPERS OF LAB: Diane McCarron JD729,Sandy Black JD706, Joyce Valenti, Michael J. Dupre, Judith Hassler
Experiment 9 Amino Acids and Proteins
Experiment 9 Amino Acids and Proteins Proteins are very important biological molecules, with many possible functions. Enzymes are proteins that catalyze biological reactions. There are transport proteins
Student Manual. Background STUDENT MANUAL BACKGROUND. Enzymes
Background Enzymes Enzymes are typically proteins (some nucleic acids have also been found to be enzymes) that act as catalysts, speeding up chemical reactions that would take far too long to occur on
ENZYMES: BIOLOGICAL CATALYSTS OF LIFE
Potential Energy Lab 6 ENZYMES: BIOLOGICAL CATALYSTS OF LIFE OBJECTIVES Define catalyst, enzyme, activation energy, enzyme-substrate complex, substrate, product, active site, denaturation, and cofactor;
Exploring Mitosis Lab IMPORTANT LEARNING OBJECTIVES INTRODUCTION
Exploring Mitosis Lab Today s lab will give you a chance to get an up-close view of mitosis, the process by which eukaryotic cells divide to generate identical daughter cells. We re using garlic (A. sativium)
Chemistry 151 Last Updated: Dec Lab 2: Measurements & Chemical Changes
Chemistry 151 Last Updated: Dec. 2013 Lab 2: Measurements & Chemical Changes Introduction This lab will serve as an introduction to some of the basic techniques and skills you ll be using in this course
Carbohydrates. Objectives. Background. Experiment 6
1 of 6 3/15/2011 7:27 PM Experiment 6 Carbohydrates Objectives During this experiment you will look at some of the physical and chemical properties of carbohydrates. Many of the carbohydrates, especially
Burping Yeast: An Investigation of Cellular Respiration
Burping Yeast: An Investigation of Cellular Respiration Student Materials Introduction... 2 Lab Protocol... 4 Data Collection Worksheet... 6 Pre-Lab Questions... 7 Post-Lab Questions and Analysis... 8
Exploring Mitosis Lab IMPORTANT LEARNING OBJECTIVES INTRODUCTION cell cycle interphase division (mitosis)
Exploring Mitosis Lab Today s lab will give you a chance to get an up-close view of mitosis, the process by which eukaryotic cells divide to generate identical daughter cells. We re using garlic (A. sativium)
CCMR Educational Programs
CCMR Educational Programs Title: Date Created: July 21, 2006 Author(s): Appropriate Level: Abstract: Time Requirement: Frank La Gatta Esterfication Regents and Honors Chemistry An ester is produced when
To understand osmosis, we must focus on the behavior of the solvent, not the solute.
GCC CHM 130LL Osmosis and Dialysis Purpose: The purpose of this experiment is to observe the closely related phenomena of osmosis and diffusion as it relates to dialysis. It is hoped that you will be able