Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK

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1 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Microbiology Department Contact: Alison M Cabot Jersey General Hospital Tel: +44 (0) Glouchester Street a.cabot@health.gov.je Jersey Website: St Helier JE1 3QS Testing performed at the above address only Sterile fluids Cerebrospinal fluid Wound swabs/ pus samples/ drains /tissues/ breast milk Swabs (nasal, ear, eye, mouth & dental, skin and throat). Whole blood and aspirates in blood culture bottles DETAIL OF ACCREDITATION diagnosis Isolation of microorganisms of clinical significance Culture and examination of Aspirates MBROUS025, Examination of Peritoneal Dialysis Fluid from Continuous Ambulatory Peritoneal Dialysis Patients MBROUS026 Examination of Cerebrospinal fluid MBROUS027 Culture of wounds, Tissue, Abscesses & Drainage specimens MBROUS024 Nasal swab culture MBROUS016, Ear swab culture MBROUS017, Eye swab culture MBROUS018, Mouth and dental specimen culture MBROUS019, Skin swab culture MBROUS023 Throat swab culture MBROUS015 Blood culture using the Becton Dickinson Bactec FX with continuous monitoring. Blood Cultures MBROUS030 Use and Maintenance of the Bactec FX MSEQUS022 Assessment Manager: JP Page 1 of 8

2 Blood bags Genital specimens Respiratory samples Urine Intravascular cannulae tips Sterile fluids, Cerebrospinal fluid diagnosis Isolation of microorganisms of clinical significance White blood cells (quantification & differentiation) Microorganisms Standard bacterial culture identification. Culture of Blood Bags MBROUS034 Culture of the male genital specimen and identification of pathogens MBGENS002 Culture of female genital specimen and identification of pathogens MBGENS003 Processing and reporting specimens from GUM & Brook clinics. MBGENS004 Culture of sputum, NPA, and ETT tubes. MBROUS037 Microscopy[y using an inverted microscope. Culture onto Oxoid Brilliance UTI clarity chromogenic agar. Examination & Culture of Urine MBURIS002 Semi-quantitative method Culture of intravascular cannulae tips MBROUS028 Manual cell count CSF SOP, MBROUS027 Gram's stain and manual culture. M-B-ROU-S001 Assessment Manager: JP Page 2 of 8

3 Faeces diagnosis Enteric sample culture and sensitivity testing for Salmonella, Shigella, E.coli 0157, Campylobacter, Vibrio, Yersinia and other food poisoning organisms. Standard bacterial culture identification & microscopy Oxoid Brilliance Salmonella selective agar. The Isolation of Pathogens found in the Gastrointestinal Tract MBFAES001 Identification of Campylobacter spp. MBFAES011 Identification of E.coli 0157 MBFAES015 Identification of Vibrio spp, Aeromonas spp. And Plesiomonas spp. MBFAES015 Identification of Yersinia enterocolitica MBFAES016 Identification of Salmonella and Shigella spp. MBFAES019 Faeces Testing for the presence of C.difficile Techlab C.difficile Quik Chek Complete, Alere C.difficile testing MBFAES014 Cepheid GeneXpert Real Time Polymerase Chain Reaction Cepheid GeneXpert C.difficile MSFS015 Assessment Manager: JP Page 3 of 8

4 Faeces Sputum/ Broncho-alveolar lavage/ pleural aspirate/ pus or tissue samples Swabs / Sputum/ Urine Corneal scrapings, contact lenses and contact lens solution Urine diagnosis Examination for the presence of ova, cysts and parasites. Detection of acid-fast bacilli Isolation of Methicillin resistant Staphylococcus aureus (MRSA) Investigation of eye infections for bacterial and fungal pathogens. Detection of Schistosoma haematobium ova Direct microscopy after faecal concentration. Detection of Ova, Cysts and Parasites in Faeces MBFAES003 Direct stain for Crytosporidia The examination of Faeces using Auramine-Phenol staining MBFAES004 Identification Of Tapeworm Proglottids MBFAES009 Auramine-phenol staining and fluorescence microscopy Investigations of specimens for Mycobacterium species MBMTBS001 Culture using Oxoid Brilliance MRSA 2 agar Bacteriology Specimen reception MSADMS017 Detection and identification of MRSA MBROUS031 Examination of corneal scrapings, contact lenses and contact lens solution MBROUS010 Examination of urine for the presence of Schistosoma haematobium MBURIS002 Assessment Manager: JP Page 4 of 8

5 diagnosis Bacterial Isolates Identification of pathogens Biomerieux API The Use of API System of Identification MBROUS011 Staphaurex Oxoid DNase agar Prolab Rabbit coagulase plasma Catalase test (hydrogen peroxide) Identification of Staphylococcus spp. MBROUS002 Prolab Streptococcus grouping kit. Oxoid bile aesculin. Identification of Streptococcus spp. MBROUS003 Urea tubes ß-Glucoronidase (PGUA) diagnostic tablets Prolab Oxidase reagent Identification of Enterobacteriaceae MBROUS004 Pro-lab Oxidase reagent Identification of non-fermenters MBROUS005 Catarrhalis test disk. Identification of Haemophilus spp. And Moraxella spp. MBROUS006 Pro-lab Oxidase reagent Identification of Coryneform and related bacteria MBROUS007 Assessment Manager: JP Page 5 of 8

6 diagnosis Bacterial Isolates Identification of pathogens Detection and identification of methicillin resistant Staphylococcus aureus MBROUS031 Isolation and identification of anaerobic bacteria MBROUS032 Identification of Neisseria species MBROUS040 Identification of Listeria ap. MBROUS041 Identification of Bacillus sp. MBROUS042 Bacterial isolates Antibiotic sensitivity testing British Society for Antimicrobial Chemotherapy (BSAC) BSAC Disc susceptibility method MBROUS012 Use of MIC gradient strips MBROUS014 Direct urine BSAC disc susceptibility method MBURIS005 Assessment Manager: JP Page 6 of 8

7 Serum unless otherwise stated Serum Serum Faeces / swabs / urine / respiratory secretions / sputum Serological examination activities for the purpose of clinical diagnosis Hepatitis B surface antigen Hepatitis B core antibody Hepatitis B surface antibody Hepatitis C antibody Hepatitis C antigen Hepatitis A IgG Hepatitis A IGM HTLV I/II HIV Ag/Ab Syphilis CMV IgG CMV IgM Antimicrobial assay Vancomycin Epstein-Barr virus (VCA IgG, IgM & EBNA IgG). Varicella zoster IgG Toxoplasma IgG & IgM CMV IgM Measles IgG Mumps IgG Rubella IgG Qualitative and semi quantitative Non-Treponemal test RPR test. Norovirus GI/GII C.difficile toxin gene/ presumptive 027 ribotype MRSA/Staphylococcus aureus Abbott Architect automated chemiluminescent micro particle immunoassay analyser. Abbott Architect Testing samples MSEQUS112 Abbott Architect testing antibiotic levels MDEQUS113 Biomerieux mini-vidas automated analyser for the quantitative detection of antibodies to viruses, using the enzyme linked fluorescent assays. Use of Biomerieux mini-vidas MSEQUS105 Plasmatec RPR test kit Detection of virus by real-time polymerase chain reaction using the Cepheid GeneXpert GeneXpert Norovirus MVSFCS014 GeneXpert C.difficile MVSFCS015 GeneXpert SA nasal complete MVSFCS017 Assessment Manager: JP Page 7 of 8

8 Faeces / swabs / urine / respiratory secretions / sputum Serological examination activities for the purpose of clinical diagnosis Influenza A/B and respiratory syncytial virus Carbapenemase screening Chlamydia / Gonorrhoea Mycobacterium tuberculosis Abbott Architect automated chemiluminescent micro particle immunoassay analyser. Detection of virus by real-time polymerase chain reaction using the Cepheid GeneXpert GeneXpert Flu A, B, RSV PCR MVSFCS011 GeneXpert Carbapenemase (Carb R) MVSFCS016 GeneXpert CT & GC MVSFCS013 GeneXpert TB PCR MVSFCS012 Faeces Detection of Rota-Adeno virus by Biomerieux BioNexia Rota-Adeno qualitative immunochromatographic test MVSFCS011 Alere BinaxNOW qualitative immunochromatographic assays MCR&RS009 Nasal washes/ nasopharyngeal swabs Urine Genital, oral & rectal swabs. Urine and peritoneal fluid Detection of respiratory syncytial virus (RSV) fusion protein antigen Detection of Legionella pneumophilia serogroup 1 antigen Detection of Streptococcus pneumoniae Detection of Chlamydia trachomatis & gonococcus Alere BinaxNOW RSV card Alere BinaxNOW Legionella Urinary antigen card qualitative immunochromatographic assay Alere BinaxNOW Streptococcus pneumoniae antigen test Strand displacement amplification using the Becton Dickinson ProbeTec ET BD ProbeTec amplified DNA assay MVSFCS002 END Assessment Manager: JP Page 8 of 8

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