Turnaround Time (from time received in the laboratory to reporting) Up to 2 days for microscopy, 8 weeks for initial culture results.

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1 MICROBIOLOGY IN HOUSE TURNAROUND TIMES : Non-Blood Ascitic fluid Up to for full result Broncho-alveolar lavage Up to for full result Chlamydia (eyes) Up to Chlamydia NAAT (urine) Up to CSF cryptococcal antigen Up to 2 days CSF M, C & S Up to for full result Ear swab Eye swab Faeces C&S, C.difficile toxin, OCP for full result (Parasites) Faeces - C.difficile toxin only 24 hours Fluids Up to for full result High Vaginal swab Up to 4 days Helicobacter pylori antigen Up to 2 weeks Legionella & Pneumococcal Antigen (Urine) Up to 2 working days (ed Mon-Fri only) MRSA screen (nose, throat, groin) Mycology (skin scrapes, nail clippings, hair samples)) Nasopharyngeal aspirate (RSV) Peritoneal fluid Pernasal swab (Whooping cough) Fluids Semen (C&S) Sputum culture (C&S) Sputum for Acid fast bacilli (AFBs) Early morning specimens preferred. Throat swab Tips CVC, ETT etc Urine early morning urine for AFBs (Send on 3 consecutive days) Urine midstream / catheter (C&S) Wound swab Up to 5 weeks Up to 2 days (not tested on call) Up to for full result Up to 8 days Up to for full result Up to 2 days for microscopy, 8 weeks for initial culture results. Up to 8 weeks for initial culture results Page 1 of 5

2 performed: Blood Antenatal screen (Rubella, HIV, Hepatitis B, Syphilis) Antibiotic assays Anti-streptolysin O titre (ASOT) Aspergillus precipitans Avian precipitans Blood cultures CMV IgG antibody CMV IgM antibody Cryptococcal antigen HIV antibody (initial screen) Hepatitis A IgM antibody Hepatitis A total antibody Hepatitis B surface Antigen Hepatitis B core antibody Hepatitis B surface antibody Hepatitis C antibody Measles IgG antibody Mumps IgG antibody Mycoplasma antibody Rubella IgG antibody Rubella IgM antibody Syphilis antibody (screen) Toxoplasma IgG antibody Varicella-Zoster Virus IgG *Urgent requests tested within 2 days 24 hours 5 days 2 days 3 days * * Page 2 of 5

3 16s PCR Acanthamoeba Culture Adenovirus PCR Alphavirus serology Amoebic Antibodies Anaerobes / Actinomyces ID Anthrax Serology Antiviral Assay - Aciclovir Antibiotic Assay - Amikacin Antibiotic Assay - Benzylpenicillin Antibiotic Assay - Ceftazidime Antibiotic Assay - Chloramphenicol PRE / Antibiotic Assay - Ciprofloxacin, Antibiotic Assay - Colistin Antibiotic Assay - Cycloserine Antibiotic Assay - Daptomycin Antibiotic Assay - Ertapenem Antibiotic Assay - Flucloxacillin Antifungal Assay - Flucytosine Antiviral Assay - Gancyclovir Antibiotic Assay - Gentamicin Antibiotic Assay - Linezolid Antibiotic Assay - Meropenem Antibiotic Assay - Moxifloxacin Antibiotic Assay - Rifampicin PRE / POST Antibiotic Assay - Streptomycin PRE / POST Antibiotic Assay - Teicoplanin Antibiotic Assay - Tobramycin Antibiotic Assay - Vancomycin Anti Strept Serology Aspergillus PCR Bacillus anthracis Bacterial Susceptibility Bordetella pertussis Ab Borellia Burgdorferi B. pertussis Antibody Brucella ID Brucella Serology Campylobacter Serology (Abs) C. difficile Culture C. difficile Ribotyping Candida Antigen & Antibody Chikungunya Chlamydia Antibody Chlamydia LGV PCR CJD CMV DNA PCR Coccidioides Serology Cockatiel Serology Coronavirus PCR Coxiella Burnetti (Q Fever) Serology Cryptococcal serology Gt Ormond Street Hospital, London London School of Hygiene and Tropical Medicine Anaerobe Reference Laboratory, Cardiff Biochemistry Antibiotic Resistance & Monitoring Laboratory, Colindale See Lymes Disease Veterinary Laboratory Agency, Weybridge Brucella Special Diagnostic Unit, Liverpool Royal Preston Hospital, Lancashire See Hanta virus See LGV National CJD Surveillance Unit, Edinburgh / Page 3 of 5

4 CSF-CJD CSF Viral PCR CSF-Whipples PCR Cysticerocosis serology Dengue Fever Diphtheria ID E. Coli Serology EBV PCR (Quantitative) EBV Serology IgG, IgM, EBNA Enterococcus ID Enterovirus IgM (Coxsackie) Enterovirus PCR (Coxsackie) Eye viral PCR screen Faeces Virology PCR Fasciola serology Filaria serology Flavivirus Serology Fungal ID / Susceptibility Galactomanann Genital Ulcer PCR See CJD See Whipples Refer to Flavivirus serology West Park Hospital, Epsom See Norovirus Giardia Serology Gp A Streptococcus Typing H. Influenzae B Abs Haemophilus / Pneumococcal / Tetanus AB Haemophilus Culture ID / Typing Haemophilus PCR Hanta Virus HAV (RL) HBV PCR (Quant) HBV genotypic resistance HBV sequencing / genotyping HBV Viral Load (Occ Health) HCV Referral HCV Genotyping HCV PCR / Viral load (Quant) HDV (DELTA) Referral HDV RNA Viral load Herpes IgG Herpes Simplex PCR & VZV DNA PCR HEV Serology Histoplasma serology HIV (Maternal Transmission) HIV Referral HIV Genotype Resistance HIV PCR Viral load HIV 1 Proviral DNA HIV (STARHS) HTLV SEROLOGY Human Herpes 6 DNA PCR Human Herpes 7 DNA PCR Human Herpes 8 DNA PCR Hydatid Serology JCV PCR JCV Serology Legionella Antigen Referral Leishmania serology Leptospira (Weils) Serology LGV (lympho granuloma venerium) PCR Royal Infirmary HPA West Midlands, Heartlands Hospital, Birmingham Mycology Reference Laboratory, Bristol Leptospira Reference Unit, Hereford Page 4 of 5

5 Listeria ID & typing Lyme / Borreliosis disease Measles IgG & IgM Measles IgM Measles PCR MecA PCR Meningococcal PCR Meningococcal Ref Microsporidial PCR MRSA Typing Mumps IgG & IgM Mumps IgM Mumps PCR Mycobacterium ID Mycobacteria in Blood Mycoplasma / Ureaplasma Neisseria gonorrhoea Nocardia ID Norovirus PCR Pan Valentine Leukocidin (PVL) Staph Parasitology Parovirus IgG Parovirus IgG & IgM Parovirus IgM Parvovirus (QUAL) PCR Parvovirus (QUANT) PCR Pertussis Antibody Phlebovirus serology Pneumococcal Antibody Pneumococcal PCR Pneumococcus Typing Polio Antibodies Q Fever Quantiferon Respiratory PCR Screen Rickettsia Rubella IGM (RL) Salmonella ID / Typing Schistosomal Antibodies Shigella ID Staph aureus Streptococcal ID (Not Enterococcus spp) Streptococcal typing Starhs (HIV) Swine Flu antibodies (GBS) Syphilis Referral Dept for Bioanalysis & Horizon Technologies, Colindale Refer to Coxiella Burnetti Homerton Hospital, London Royal Preston Hospital, Lancashire TB PCR TB Quantiferon Toxocara Serology Toxoplasma (Ref lab) Treponemal (Syphilis) referral Trichinella serology IFAT Trypanosoma serology (Ab detection) VZV IgG & IgM (Ref Lab) Whipples PCR Zika Homerton Hospital, London Toxoplasma Reference Laboratory, Swansea Gt Ormond Street Hospital, London Page 5 of 5

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