Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK

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1 ccredited to Laboratory locations: Schedule of ccreditation United Kingdom ccreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Brighton and Sussex Pathology The Royal Sussex County Hospital djacent Thomas Kemp Tower Eastern Road Brighton BN2 5BE United Kingdom Contact: John McBride Tel: +44 (0) John.McBride@bsuh.nhs.uk Website: below s covered by the organisation and their relevant activities details ctivity code ddress The Royal Sussex County Hospital djacent Thomas Kemp Tower Eastern Road Brighton BN2 5BE United Kingdom Local contact John McBride See above Full Microbiology diagnostic service including: Bacteriology Mycology Parasitology Serology Molecular testing Site activities performed away from the locations listed above: details ctivity code ddress Princess Royal Hospital Lewes Road Haywards Heath RH16 4EX Local contact John McBride See above Isolation and first stage identification for MRS only B ssessment Manager: HL Page 1 of 11

2 Schedule of ccreditation United Kingdom ccreditation Service ccredited to DETIL OF CCREDITTION HUMN TISSUES ND FLUIDS Microbiological examination clinical diagnosis Routine samples: spirates Isolation of microorganisms of clinical significance Manual inoculation and media culture using: procedures: LIC-MIC-INOC Fluids Pus Samples from sterile sites Bone Cellular and fluid material contained on swabs, catheter tips and intrauterine contraceptive devices Genital samples Semen Tissues Cellular and fluid material contained on swabs Screening for Methicillin- Resistant Staphylococcus aureus (MRS) Manual inoculation and media culture using: SOP LPc-MBP-MRS, B Rectal swab, Faeces and Screening for Resistant organisms VRE and CRE Manual inoculation and media culture using SOP s: LPc-MBP-VRE LPc-MBP-CRE Blood Joint fluids Isolation of microorganisms of clinical significance Liquid media culture Using SOP: LPc MBP-BloodCul and BD BCTEC Sterile samples - joint fluid, CSF, pleural effusion, BL, ascites, biopsies, tissue samples, corneal scrapes, CPD Detection, identification and quantification (inc. differential) of: White blood cells Red blood cells Bacterial cells Microscopy & staining (Gram and differential) using: LPc-MTP-STINS ssessment Manager: HL Page 2 of 11

3 Schedule of ccreditation United Kingdom ccreditation Service ccredited to HUMN TISSUE ND Microbiological examination CSF Isolation of microorganisms of clinical significance Manual inoculation and media culture using: SOP LPc-MBP-CSF Bronchoalveolar lavage Bronchial aspirate, brushings & washings Cellular and fluid material contained on swabs Endotrachael tube specimens Nasopharyngeal aspirate Pleural fluid Sputum Isolation of microorganisms of clinical significance Detection, identification and quantification of: Manual inoculation and media culture using: SOP LIC-MIC-INOC Staining (inc. Gram) and microscopic examination using: spirates White blood cells SOP: LPc-MTP-STINS Fluids CPD Corneal scrapes Eye (specimens from) Tissues BL Red blood cells Bacterial cells Yeast cells and fungal hyphae Sterile fluids incl pleural fluids, CSF and bone marrow Sputum, BL, urines, pus, tissues, non-sterile fluids Detection of Mycobacterium Microscopy and culture LPc-MBP-TB ssessment Manager: HL Page 3 of 11

4 Schedule of ccreditation United Kingdom ccreditation Service ccredited to HUMN TISSUE ND Microbiological examination Detection, identification and quantification of: White blood cells Red blood cells Squamous epithelial cells Bacterial cells Hyaline casts Granular casts Fatty casts Waxy casts Cellular casts Yeasts utomated urinalysis, and manual examination using: LPc-MBP-URINE using Menarini sedimx Isolation of microorganisms of clinical significance Manual and/or automated inoculation and media culture using: SOP Lpc-MBP-Uri utomated using LPc-MBP-URINE and Mast URI System Hair Nail Skin Isolation and identification of clinically significant fungi Manual inoculation, media culture, and macroscopic & microscopic examination using: SOP LPc-MYP-MYCOL Clear adhesive tape Detection of Enterobious vermicularis larvae and ova Microscopic examination using SOP: LPc-MPP Genital site swab Detection of Trichomonas vaginalis trophozoites Light/phase contrast microscopy using SOP: LPc-MBP-GENITL ssessment Manager: HL Page 4 of 11

5 Schedule of ccreditation United Kingdom ccreditation Service ccredited to HUMN TISSUE ND Microbiological examination Cultures isolated in-house from all samples listed above Identification of microorganisms of clinical significance Manual and/or automated biochemical methods using: LIc-MIC-Inoc, LPc-MS-SENS nd/or Matrix-assisted laser desorption/ionisation using Maldi-ToF and SOP: LPC-MBI-MLDITOF Bacterial cultures isolated inhouse from all samples listed above ntimicrobial susceptibility testing of clinically significant bacteria Manual antimicrobial susceptibility testing using BSC methodology and in-house procedures LIc-MIC- Inoc, LPc-MS-SENS MESOP 24 Disc diffusion & gradient minimum inhibitory concentration determination Female genital tract swabs Microscopic diagnosis of bacterial vaginosis Gram stained microscopic examination using: LPc-MBP-GENITL Faeces, tissues, biopsies, pus, sputum, duodenal/jejunal aspirate, bronchoalveolar lavage Detection of ova, cysts and parasites (OCP) Unstained and iodine stained direct microscopic examination and/or Formol-ether faecal concentration (faeces only) and microscopic examination Using SOP: LPc-MPP-PR Faeces Detection of C. difficile toxin & B and glutamate dehydrogenase (GDH) Wampole QuikChek complete by rapid membrane enzyme immunoassay and SOP: LPc-MB- CD-GDHTOXB Blood Detection of Helicobacter pylori Immunocard methodology and proceure LPc-MB-HPntigen ssessment Manager: HL Page 5 of 11

6 Schedule of ccreditation United Kingdom ccreditation Service ccredited to HUMN TISSUE ND Microbiological examination Faeces Detection of Cryptosporidium oocysts uramine fluorescent microscopic examination using: LPc-MPP-PR Detection of Schistosoma haematobium ova Molecular examination clinical diagnosis Microscopic examination using inhouse procedure: LPc-MPP-PR Nasopharyngeal aspirate Nasopharyngeal swab Detection or exclusion of: Influenza Influenza (H1N1) Influenza B Respiratory syncytial virus /B Rhinovirus Coronavirus NL63, 229E, OC43, HKU parainfluenza 1, 2, 3, 4 human metapneumovirus /B Bocavirus denovirus Enterovirus Parechovirus Mycoplasma pneumoniae utomated Molecular Testing (PCR) using SOP s: LP-MOT-FTDEXT, LP-MOT-FTDMSTERMIX, LPC- MOT-FTDLC480 by multiplex polymerase chain reaction (PCR) using Fast Track Diagnostics assays Genital swab Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, herpes simplex virus 1, 2 and Treponema pallidum ssessment Manager: HL Page 6 of 11

7 Schedule of ccreditation United Kingdom ccreditation Service ccredited to HUMN TISSUE ND Molecular examination utomated Molecular Testing (PCR) using: Fast Track Diagnostics assays (cont d) Blood, swabs and CSF Detection of vesicular rash causing viruses (herpes simplex virus 1, 2 and varicella-zoster virus). Swab, blood, and faeces Human herpes virus 6, 7, parvovirus B19, enterovirus and measles virus Swabs, fluids and tissues. Herpes simplex virus 1, 2, varicella-zoster virus, adenovirus and Chlamydia trachomatis Blood Genital site swab, urine CSF Faeces Detection and quantification of cytomegalovirus, Epstein-Barr virus and adenovirus Detection and confirmation of Neisseria gonorrhoea Detection of viral meningitis (herpes simplex virus 1, 2, varicella-zoster virus, enterovirus, mumps virus and parechovirus Detection of viral gastroenteritis (norovirus G1, G2, astrovirus, rotavirus, adenovirus and sapovirus). ssessment Manager: HL Page 7 of 11

8 Schedule of ccreditation United Kingdom ccreditation Service ccredited to HUMN TISSUE ND Molecular examination Faeces Detection of Salmonella spp, Shigella spp, Verotoxin, Campylobacter spp, Giardia spp and Cryptosporidium spp LPc-MBP-FECESPCR using EnteroBio method Genital site swab, urine Detection of Chlamydia trachomoatis and Neisseria gonorrhoea LIC-MOT-EXVIPERUSER by nucleic acid amplification tests (NTS) using BD Viper Genital swab, urine Detection and confirmation of Chlamydia trachomatis LIC-MOT-EXRGQMNUL by polymerase chain reaction (PCR) using Qiagen assays Blood, Detection and quantification of CMV Blood Detection and quantification of Epstein-Barr virus Swabs, blood, csf and samples from normally sterile sites Detection of herpes simplex 1 and 2 Blood Detection of parvovirus B19 Blood, swabs Detection of varicella zoster virus ssessment Manager: HL Page 8 of 11

9 Schedule of ccreditation United Kingdom ccreditation Service ccredited to HUMN TISSUE ND Serological examination clinical diagnosis Plasma Serum Microbial serology for detection of: Hepatitis IgM and IgG Hepatitis B surface antigen Hepatitis B core antibodies (IgM and Total) Hepatitis B antigen Hepatitis B antibody Hepatitis C antibodies Hepatitis C core antigen HIV p24 antigen, HIV 1 and 2 antibodies HTLV I / II antibodies Treponemal antibodies CMV IgG Enzyme Immuno-ssay (EI) LPc-MVS-RCHITECT using bbott rchitect Quantification of: Hepatitis B surface antibodies Hepatitis C core antigen Rubella IgG Plasma/Serum Hepatitis B core antibodies Hepatitis B surface antigen HIV1 & 2 antibodies and P24 antigen Lyme IgM Lyme IgG Measles IgG Mumps IgG BioMerieux MiniVidas and procedure: LIC-MVS- EXVIPROCMN ssessment Manager: HL Page 9 of 11

10 Schedule of ccreditation United Kingdom ccreditation Service ccredited to HUMN TISSUE ND Serological examination Plasma Serum Microbial serology for detection of: EI DiaSorin Liaison XL using LIC-MVS-EXLIXLUSERMN Parvovirus IgG Parvovirus IgM CMV IgG CMV IgM CMV IgG avidity Epstein-Barr IgM HSV 1 IgG HSV 2 IgG Measles IgG Rubella IgM Toxoplasma IgM Toxoplasma IgG Treponema total antibodies VZV IgM EBN IgG EBV viral capsid IgG Quantification of: VZV IgG Rubella IgG Microbial serology for detection of: Measles IgG Measles IgM Mumps IgM Mumps IgG EI using Chorus and procedure: LIC-MVS-EXCHORUSOP Quantification of: HBV viral load HIV-1 viral load HCV viral load LIC-MOT-EXM2000RT using M2000rt Detection of Cryptococcal antigen LP-MVS-CRYPTOG using Meridian Premier kit ssessment Manager: HL Page 10 of 11

11 Schedule of ccreditation United Kingdom ccreditation Service ccredited to HUMN TISSUE ND Serological examination Plasma Serum Detection of Hepatitis C antigen and antibody LIC-MVS-EXBIOHCV using BioRad Monolisa Detection and differentiation of antibodies to HIV-1 and HIV-2 LIc-MVS-HIVTYPEQUICK using Geenius HIV 1&2 BiSpot Detection of non-treponemal antibodies Reiters Protein Reaction LPc-MVS- RPR using Werfen Biokit Detection of antibodies to Treponema pallidum LPc-MVS-TPHV2 using Werfen Biokit Plasma/Serum Quantification of nti- Streptolysin O LIC-MVS-EXSO using Werfen Biokit Detection of Legionella pneumophila serogroup 1 antigen LIa-MB-BinaxLegPne using BinaxNOW Legionella END ssessment Manager: HL Page 11 of 11