Ashley Novais EDUCATION. ICVS, University of Minho, Portugal
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1 Ashley Novais Braga, Portugal (+351) EDUCATION University of Minho (Portugal) Ph.D. in Health Sciences, (1 st edition of the inter-university PhD program PhDOC ) December 2013 Dissertation: Unraveling the role of neudesin in the central nervous system. Dissertation advisor: João Carlos Sousa, Ph.D. M.Sc. in Molecular Genetics, December 2009 Master s Thesis: Establishment and characterization of in vivo models of glial tumors. Dissertation advisor: Rui Manuel Reis, Ph.D. B.S. Applied Biology, September 2007 University of Porto (Portugal) Post-graduation in Forensic Sciences, September 2008 TecMinho September Present Post-graduation in Project Management Training Programme in Pharmaceutical Medicine (Portugal) August 2017 Clinical investigator certificate (CLIC), Direção Geral de Veterinária (Portugal) September 2008 Felasa C certificate in Laboratory Animal Science, RESEARCH EXPERIENCE INL, Braga, Portugal Research Fellow Sep Present Project: Implantable systems for multimodal theranostics. Improve configuration, geometry and bio-compatibility of implantable microelectrodes and sensors for the monitoring of neurologic activity. Wavemetrix, London, UK Jan 2017-September 2017 Freelance research analyst in media opinion. Research analyst ICVS, Portugal - INSERM, Paris, France - NeuroSpin-CEA, Saclay, France Researcher In vivo, ex vivo and spectroscopy and functional MRI data correlation with corticosterone levels and behavior scores of rats exposed to chronic stress. Depart. Pharmacology, University of Athens, Greece PhD student 2012 Training in HPLC technique for the quantification of monoamines and amminoacids in mouse brain samples. ICVS, University of Minho, Portugal Project: Neudesin - characterization of a novel neurotrophic factor. PhD student
2 AWARDS & HONOURS Travel Grant from IBRO for the Society for Neurocience", November 11th to 17th 2016, San Diego, USA. In Europe Short Stay award from IBRO. To join the lab of Sebastian Meriaux, at Neurospin, Saclay, France, Best poster in the "international meeting of Steroids in the Nervous system" February 14 th to 18 th 2015, Torino, Italy. Travel Grant for the "International Meeting Steroids and Nervous System" February 14 th to 18 th 2015, Torino, Italy. Travel Grant for the "Optogenetics symposium" in May 3 th, 2014, Oxford, UK. SKILLS & ACTIVITY Research skills: Animal models: Mouse knockout colony management. Rodent handling. Mouse and rat behavioral assessment of emotional and cognitive dimensions. Determination of embryonic timepoints. Vaginal smears collection and estrous cycle evaluation. Acute and chronic stress paradigms. Intra-peritoneal, sub-cutaneous injections and drug preparation. Blood collection by tail venipuncture. Cardiac perfusion, organ collection. Rat stereotaxic cannulas implantation. Cellular and molecular biology: Frozen brain section immunohistochemistry and paraffin included tissue immunohistochemistry. Confocal, fluorescence, stereological and neuron morphometric (Golgi-cox staining) microscopy. RNA extraction from small amounts or normal amounts of tissue. Gene Expression by qpcr. Competitive immunoassay (ELISA) quantification. BrdU labeling protocols. DNA cloning with plasmid vectors. Other techniques: Monoamines and aminoacids quantification by high-performance liquid chromatography (HPLC). Chick Chorioallantoic Membrane assay. Cell line culture management. Primary cultures of rat hippocampal neurospheres. Primary cultures of rodent astrocytes. Transferable skills: Written & Oral Communication; Research & Information Management; Project Management & Organization; Analysis & Problem-Solving; Interpersonal & Leadership Skills; Self-Management & Work Habits. Tutorial skills: Teaching assistant in the hands-on laboratory sessions of the "The fundamentals in neuroscience" course, 5 th, 6 th, 7 th, 8 th and 9 th editions. ICVS-3B's. University of Minho. Braga, on Co-supervision of several medical students in summer lab rotations and master thesis work. 2
3 Technical skills: MS office (Word, Excel, Powerpoint) & Endnote GraphPad Prism & SPSS Illustrator & Photoshop & AutoCad MatLab Excellent Excellent Solid Beginner Organization skills: Participation in the yearly ICVS open door s day to the community; Staff of the 5 th edition SINAL, Braga 2010 RESEARCH PROJECTS Norte2020 Regional Operational program: Frontiers of technology for theranostics of cancer, metabolic and neurodegenerative diseases. Active team member. FCT Scientific research project Grant: "Adaptive and maladaptive response to stress: longitudinal characterization in brain imaging and molecular correlates. Active team member. Bial pharmaceuticals grant: "Temporal modulation of the subventricular zone neural stem cell niche by choroid plexus cerebral spinal fluid derived factors". Team member. FCT Scientific research project Grant: "Characterization of neudesin, a novel neurotrophic factor". Project management and active team member. ADVANCED COURSES Advanced Course: European college of Neuropsychopharmacology - ECNP Seminar Greece ECNP Seminar, Nafplion, Greece - November 2012 Post-Graduate course: Regenerative Medicine Center for Neuroscience and Cell Biology, Faculty of Medicine, University of Coimbra, Coimbra, Portugal May 2010 Post-Graduate course: Cell Damage and Repair - Centre of Ophthalmology-University of Coimbra, Biomedical Institute for Research in Light and Image, Coimbra, Portugal May 2010 Post-Graduate course An integrative approach to cell analysis - Life and Health Sciences Research Institute (ICVS), University of Minho. Braga, Portugal, August March, 2010 Post-Graduate course Host and pathogen interaction -Life and Health Sciences Research Institute (ICVS), University of Minho. Braga, Portugal, August March 2010 Post-Graduate course: Molecular oncology Portuguese Institute of Oncology, IPOLFG, EPE, Lisbon, Portugal February 2010 Post-Graduate course: Chronic inflammation and immunomodulation advanced course - Faculty of Medical Sciences, Chronic Diseases Research Center, Lisbon, Portugal February 2010 Post-Graduate course Research methodologies - Life and Health Sciences Research Institute (ICVS), University of Minho. Braga, Portugal, August December 2009 Post-Graduate course Bioinformatics in health sciences - Life and Health Sciences Research Institute (ICVS), University of Minho. Braga, Portugal, August November
4 Post-Graduate course Fundamentals of genetics, development and neoplasia - Life and Health Sciences Research Institute (ICVS), University of Minho. Braga, Portugal, August October 2009 Post-Graduate course Fundamentals in neuroscience - Life and Health Sciences Research Institute (ICVS), University of Minho. Braga, Portugal, August September 2009 PUBLICATIONS & PRESENTATIONS Papers: Magalhães R, Barrière DA, Novais A, Marques F, Marques P, Cerqueira JJ, Sousa JC, Cachia A, Boumezbeur F, Bottlaender M, Mériaux S, Jay TM and Sousa N, The dynamics of stress: a longitudinal MRI study of brain structure and connectome. Accepted in Molecular Psychiatry. Impact factor: 13,314 Ferreira AC, Santos T, Sampaio-Marques B, Novais A, Mesquita SD, Ludovico P, Bernardino L, Correia- Neves M, Sousa N, Palha JA, Sousa JC and Marques F, Lipocalin-2 regulates adult neurogenesis and contextual discriminative behaviours. Accepted in Molecular Psychiatry. Impact factor: 13,314 Luis R. Jacinto, Rui Mata, Ashley Novais, Fernanda Marques and Nuno Sousa, The habenula as a critical node in chronic stress-related anxiety. Experimental Neurology (2016) doi: /j.expneurol Impact factor: 4,706 Novais A, Monteiro S, Roque S, Correia-Neves M and Sousa N, How age, sex and genotype shape the stress response. Neurobiology of stress (2016). doi: /j.ynstr Castanho TC, Moreira PS, Portugal-Nunes C, Novais A, Costa PS, Palha JA, Sousa N, and. Santos NC, The role of sex and sex-related hormones in cognition, mood and well-being in older men and women. Biological psychology (2014) 103C doi: /j.biopsycho Impact factor: 3,2 Novais A, Ferreira AC, Marques F, Pêgo JM, Cerqueira JJ, David-Pereira A, Campos FL, Dalla C, Kokras N, Sousa N, Palha JA, Sousa JC Neudesin is involved in anxiety behavior: structural and neurochemical correlates. Front. Behav. Neurosc. (2013) 7:119. doi: /fnbeh Impact factor: 3,104 Ferreira AC, Pinto V, Dá Mesquita S, Novais A, Sousa JC, Correia-Neves M, Sousa N, Palha JA, Marques F Lipocalin-2 is involved in emotional behaviors and cognitive function. Front. Cell. Neurosci. (2013) 7:122. doi: /fncel Impact factor: 4,555 Falcão AM, Marques F, Novais A, Sousa N, Palha JA and Sousa JC The path from the choroid plexus to the subventricular zone: go with the flow! Front. Cell. Neurosci. (2012) 6:34. doi: /fncel Impact factor: 4,555 Cumulative impact factor: 33,434 Oral communications: Invited speaker at Experimental & Translational Medicine Seminar, October 14 th, Ninewells Hospital & Medical School, Dundee, UK. "The role of neudesin in the brain: implication in anxiety." Selected for oral communication at the XIII Annual Meeting of the Portuguese Society for Neuroscience, 30th of May to 1st of June of 2013, Luso, Portugal. Posters: Novais A, Ferreira AC, Marques F, Sousa N, Palha JA and Sousa JC. Dimorphic modulation of membraneassociated progesterone receptor neudesin in adult hippocampal neurogenesis. Society for Neurocience (SfN), November 11 th to 17 th, 2016, San Diego, USA. 4
5 the adult hippocampal neurogenesis. NeuroConferences, December 2 nd and 3th, 2015, Braga, Portugal. Boumezbeur F, Magalhães R, Novais A, Mériaux S, Bottlaender M, Cachia A, Jay T and Sousa N. Assessment of Stress-induced Neurochemical Alterations in a Rat Model of Chronic Stress using in vivo 1 H MRS at 11.7 Tesla. 23 rd Annual Meeting of the international society for magnetic resonance in Medcare, May 30 th June 5 th, 2015, Toronto, Canada. the adult hippocampal neurogenesis a link between progesterone and anxiety?. XIV Annual Meeting of the Portuguese Society for Neuroscience, June 4 th and 5 th, 2015, Porto, Portugal. the adult hippocampal neurogenesis - a link between progesterone and anxiety?. 8th international meeting steroids and nervous system, February 14 th to 18 th, 2015, Torino, Italy Novais A, Ferreira AC, Marques F, Pego JM, Cerqueira JJ, Pereira AD, Campos FL, Dalla C, Kokras N, Sousa N, Palha JA, Sousa JC. The role of neudesin in the brain: implication in anxiety. XIII Annual Meeting of the Portuguese Society for Neuroscience, May 30 th to June 1 st, 2013, Luso, Portugal. Novais A, Ferreira AC, Marques F, Sousa N, Palha JA, Sousa JC. Neudesin, a putative neurotrophic factor, ablation impacts on post-natal developmental milestones. Society for Neurosciences (SFN), October 13 th to 17 th, New Orleans, USA. REFERENCES Joao C Sousa ICVS - University of Minho Campus de Gualtar Braga Portugal jcsousa@med.uminho.pt Sébastien Meriaux CEA / DRF / I 2 BM / NeuroSpin / UNIRS Bâtim. 145, Point Courr Gif-sur-Yvette Cedex France Sebastien.meriaux@cea.fr Nuno Sousa ICVS - University of Minho Campus de Gualtar Braga Portugal njcsousa@med.uminho.pt 5
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