Primary Mouse Cerebral Cortex Neurons V: 80% TE: 70%
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1 Primary Mouse Cerebral Cortex Neurons V: 80% TE: 70% Pictures: 9 days after electroporation Red: MAP2 Blue: GFAP Green: GFP The cells were from Embryonic Day 14 Mouse Cerebral Cortex
2 Primary Mouse Hippocampal Neurons Pictures: 3 days after electroporation Red: MAP2 Green: GFP
3 Primary Mouse Neural Progenitor Cells V: 80% TE: 60% Pictures: 7 days after electroporation Red: Phalloidin Blue: DAPI Green: GFP
4 Primary Mouse Cerebellar Granule Neurons Nucleofector (Lonza amaxa) result: Transfection Efficiency 24% TE: 65%
5 Primary Rat Cerebral Cortex Neurons V: 70% TE: 75% The cells were from Embryonic Day 16 Rat Cerebral Cortex. Pictures: 5 days after electroporation
6 Primary Rat Bulbar Neurons V: 80% TE: 75% 7 days after electroporation / The cells were from Embryonic Day 15 Rat Bulbar Neurons
7 Primary Rat Schwann cells V: 90% TE: 80%
8 Primary Rat Schwann cells V: 90% TE: 60% Pictures: 48 hours after electroporation
9 Primary Chick Dorsal Root Ganglia V: 90% TE: 40% The cells were from day 7-9 chick embryos
10 Primary Chick Dorsal Root Ganglia V: 90% TE: 40% The cells were from day 7-9 chick embryos
11 Mouse Neurospheres V: 90% TE: 75% The cells were from E13.5 mouse brain: ganglionic eminence
12 Mouse Neurospheres Neural stem cells derived from mouse SVZ 24 hours after electroporation 48 hours after electroporation 72 hours after electroporation
13 Primary Mouse Cerebral Cortex Neurons in Adherence The neurons were prepared from E15 mouse cerebral cortex. Electroporation: After 6 days in vitro (DIV) on 24-well plates Pictures: 2 days after electroporation Many robust EGFP signals suggest high transfection efficiency.
14 Primary Mouse Cerebral Cortex Neurons in Adherence The neurons were prepared from E15 mouse cerebral cortex. Electroporation: After 6 days in vitro (DIV) on 24-well plates Pictures: 2 days after electroporation *High magnification Neurites are shown clearly. Department of Neurochemistry, National Institute of Neuroscience, Japan
15 Primary Mouse Cerebral Cortex Neurons in Adherence The neurons were prepared from E15 mouse cerebral cortex. Electroporation: After 6 days in vitro (DIV) on 24-well plates Pictures: 2 days after electroporation *High magnification Neurites are shown clearly. Department of Neurochemistry, National Institute of Neuroscience, Japan
16 Primary Mouse Cerebral Cortex Neurons in Adherence V: 70% TE: 70% The neurons were prepared from E16 mouse cerebral cortex. Electroporation: After 2 days in vitro (DIV) on 24-well plates
17 Primary Mouse Cerebral Cortex Neurons in Adherence V: 60% TE: 65% The neurons were prepared from E16 mouse cerebral cortex. Electroporation: After 2 days in vitro (DIV) on 24-well plates
18 Primary Mouse Cerebral Cortex Neurons in Adherence V: 95% TE: 75% The neurons were prepared from E14 mouse cerebral cortex. Electroporation: After 5 days in vitro (DIV) on 24-well plates
19 Primary Mouse Hippocampal Neurons in Adherence V: 85% TE: 54% The neurons were prepared from E18 mouse cerebral cortex. Electroporation: After 2 days in vitro (DIV) on 24-well plates
20 Primary Mouse Microglial cells in Adherence V: 80% TE: 73% Electroporation: After 1 days in vitro (DIV) on 24-well plates, post 1-week co-culturing astrocyte and microglial cells
21 Primary Mouse Glial cells in Adherence Electroporation: After 14 days in vitro (DIV) V: 80% TE: 50%
22 Primary Rat Cerebral Cortex Neurons in Adherence V: 70% TE: 60% The neurons were prepared from E17 rat cerebral cortex. Electroporation: After 2 days in vitro (DIV) on 24-well plates Pictures: 24 hours after electroporation
23 Primary Rat Hippocampal Neurons in Adherence V: 70% TE: 70% The neurons were prepared from E16 rat cerebral cortex. Electroporation: After 2 days in vitro (DIV) on 24-well plates Pictures: 2 days after electroporation
24 Primary Rat Hippocampal Neurons in Adherence V: 70% TE: 70% The neurons were prepared from E16 rat cerebral cortex. Electroporation: After 2 days in vitro (DIV) on 24-well plates Pictures: 4 days after electroporation
25 Primary Rat Hippocampal Neurons in Adherence V: 100% TE: 50% 48 hours after electroporation The neurons were prepared from P7 rat hippocampus. Electroporation: After 11 DIV
26 Primary Rat Hippocampal Neurons in Adherence V: 100% TE: 50% 48 hours after electroporation The neurons were prepared from P7 rat hippocampus. Electroporation: After 11 DIV
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The neuron Biocytin labeled pyramidal neuron recorded in piriform cortex Discovery of the neuron (A) Reticularist Doctrine (B) Neuron Doctrine Exception.GAP JUNCTIONS between neurons Neuronal shape Dendrites
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Fig The C.S. of the Spinal Cord A ganglion is a term for a collection of perikarya located outside of the CNS. In certain regions of the spinal
Chapter 9 Nervous System The nervous system is divided into two components: The CNS - the brain and spinal cord and the PNS - the nerves emanating from the spinal cord and brain that distribute to other