MrOS Measurements. Baseline

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1 Abdominal adipose measurements Anthropometry (from QCT scans) Anthropometry Height Harpenden stadiometer Anthropometry Neck, waist, hip circumferences Anthropometry Sitting height Weight balance beam scale (digital Anthropometry scale in PO) Skeletal muscle mass (from d3- Anthropometry creatine dilution method) Abdominal aortic calcification (lateral Aortic calcification radiographs of lumbar spine) Blood Pressure Pulse Blood Pressure Resting blood pressure Blood Pressure Ankle Arm blood pressure Caffeine Caffeine Intake Cognitive Function Digit Vigilance Cognitive Function Self-reported memory change Teng Modified Mini-Mental State Eam Cognitive Function 3MS pt) Cognitive Function Trails B SAQ Mouth X-ray Periodontal Eam Probing and Attachment Levels Oral BMD Depression Geriatric Depression Scale Diet Block FFQ Diet Diet history DISH Diffuse idiopathic skeletal hyperostosis assessment DXA Hip Measures # DXA Spine Measures # DXA Whole body Measures # ** ECG ECG Fatigue Revised Piper Fatigue Scale Fatigue Pittsburgh Fatigability Scale Finite Element Analysis DXA-based hip FEA (case cohort) Finite Element Analysis QCT-based hip FEA (case cohort) Finite Element Analysis QCT-based spine FEA (case cohort) Fracture Quantative Morphometry Fracture Semi-quantative assessment Fracture WHO FRAX 10-yr fracture probabilities Fracture FORE-FRC 10-yr fracture probabilities Sleep

2 General Information Ancestry General Information Education level General Information Family questions General Information Living arrangement General Information Living arrangement- type of residence General Information Marital status General Information Occupation General Information Race General Information Social and economic status Hand Eam Hand eam (OA) + hand pain Hip Eam Range of Motion Hip OA Femoral Acetabular Impingement (FAI) Hip OA Minimal Joint Space Hip OA Joint space narrowing Hip OA Osteophytes Hip OA Sclerosis, Cysts, Defomity, Buttress Hip OA Pagent's Disease HRpQCT Measures Cross sectional moment of interia HRpQCT Measures Finite element derived failure load HRpQCT Measures Cortical porosity HRpQCT Measures Trabecular density Images DXA (Hip) Images DXA (Spine) Images DXA (Whole body) ** Images HRpQCT (radius) Images HRpQCT (tibia) Images HRpQCT (proimal calf) Images QCT (Hip) Images QCT (Spine) Images X-ray (Hip) Images X-ray (Thoracic and lumbar spine) Kyphosis Block s Kyphosis Cobb Angle Lifestyle Life Space Lifestyle Social Networks Lifestyle Stressful Life Events Lifestyle/QOL SF-12 Lifestyle/QOL SOF Functional Status (IADL) Lifestyle/QOL EQ5D Lung Function Respiratory Symptoms Questionnaire Lung Function Spirometry Medical History Back and joint health Sleep

3 Etended CVD self-reported medical Medical History conditions General self-reported medical Medical History conditions Medical History General pain Medical History Hip pain Medical History History of falls Medical History History of fractures Medical History Hospitalizations Medical History Intention to lose weight Medical History Limited activity days Medical History Rose questionnaire CVD Sensory Questions (hearing, smell, Medical History touch) Medical History Seual health Seual health- erectile dysfunction Medical History single question Medications Medication Inventory *** Moods/Feelings Goldberg Aniety Scale Personality Personality Questionnaire Physical Activity Accelerometry (Activity Monitor) Physical Activity General questions Physical Activity PASE Physical Performance 6m walk Physical Performance 400 m walk Physical Performance Instrumented balance and Gait (ISAW) ** Physical Performance Chair Stands Physical Performance Force Plate Physical Performance Grip Strength Physical Performance Narrow Walk Physical Performance Nottingham (leg power) * Physical Performance SPPB Balance Test Prostate Health AUA Symptom Questionnaire Prostate Health General questions QCT Spine, hip analysis QCT Statistical shape and density modeling (case cohort) Sleep Actigraphy Sleep Arrhythmia data from PSG Sleep EEG power spectral data from PSG Sleep Epworth sleepiness scale Sleep Functional Outcomes of Sleep Sleep Insomnia severity inde Sleep Pittsburgh Sleep Quality Inde Sleep

4 Sleep Light data (LUX)- from actigraphy Sleep Polysomnography Sleep Psg morning survey Respiratory-releated limb movement Sleep from PSG Sleep Restless legs syndrome rating scale Sleep Sleep Disorders Sleep Sleep habits Specimen Collection Blood blotter Specimen Collection Finger and toe nails Specimen Collection Serum (V4R stored locally) Specimen Collection Plasma Specimen Collection Urine- 1st morning void Specimen Collection Urine- 24 hour ** Specimen Collection Urine- spot collection Specimen Collection Whole blood Specimen Collection Stool Spondylolisthesis from spine Spondylolisthesis radiographs Tobacco & Alcohol Use CAGE Tobacco & Alcohol Use Current: frequency & quantity Trochanteric soft tissue from DXA Trochanteric Soft Tissue (case cohort) Ultrasound Hologic Sahara Vision Functional Vision Vision Self-reported visual function Adiponectin (serum, V3 is subset) Albumin (serum) Albumin (micro) (urine, case-cohort) Albumin (urine) Alkaline phosphatase (serum) alpha-ctx (urine, case-cohort) beta-ctx (urine, case-cohort) Calcium (serum) Calcium (urine, case-cohort) Chloride (serum) Chloride (urine, case-cohort) Cholesterol (serum) Complete blood count Sleep

5 C-reactive protein (serum, baseline is case-cohort, V3 is subset) Creatinine (serum, V3 is subset) Creatinine (urine, V1 is case cohort) CTX (serum, case-cohort) Cystatin-C (serum, V1 is case-cohort, V3 is subset) Cytomegalovirus seropositivity, optical density (serum, case-cohort) egfr equations (V3 is subset) Estradiol by Mass Spec (serum, casecohort) Estradiol by RIA (serum, stratified random cohort) Estrone (serum, case-cohort) Ferritin (serum) FGF23 (serum, case-cohort) Follicle Stimulating Hormone (FSH) (serum, case-cohort) Free Thyroine (FT4) (serum, casecohort) Glucose (serum, V3 is subset) Hepcidin (serum) High IFN-y density (Interferon-gamma) lipoprotein (serum) (serum, V3 is subset) IL-1β (Interleukin-1beta) (serum, V3 is subset) IL-10 (Interleukin-10, serum, casecohort) IL-6 (Interleukin-6) (serum, baseline is case-cohort, V3 is subset) Insulin (serum, V3 is subset) Irisin (serum) Iron (serum) Leptin (serum, V3 is subset) Luteinizing Hormone (LH) (serum, casecohort) Low density lipoprotein (serum) Magnesium (urine, case-cohort) Melatonin (urine) Osteocalcin (serum) Oidized LDL (serum) Phosphate (serum) Phosphatase (urine, case-cohort) Potassium (serum) Sleep

6 Potassium (urine, case-cohort) P1NP (serum, case-cohort) PTH, total intact (serum, baseline is case cohort) PTH, whole (serum, baseline is case cohort) SHBG (serum, case cohort) SHBG (serum, stratified random cohort) Sodium (serum) Sodium (urine, case cohort) Testosterone by Mass Spec (serum, case-cohort) Testosterone by RIA (serum, stratified random cohort) Thyroid Stimulation Hormone (TSH) (serum, case-cohort) TNF-a (tumor necrosis factor-alpha) (serum, baseline is case-cohort, V3 is subset) TNF-asRII (tumor necrosis factor-alpha soluble receptor II) (serum, V3 is subset) TRACPb (serum, case-cohort) Transferrin (serum) Triglycerides (serum) Uric Acid (serum, case-cohort) Vitamin D2 1,25(OH)2 (serum, casecohort) Vitamin D3 1,25(OH)2 (serum, casecohort) Vitamin D binding protein (serum, casecohort) Vitamin D2 25(OH) (serum, V1 is casecohort) Vitamin D3 25(OH) (serum, V1 is casecohort) Sleep

7 Sleep MrOS Visits Time Range N year^ March 2000 to April Interim Questionnaire July 2002 to March September 2002 to May Sleep December 2003 to March March 2005 to May March 2005 to April March 2007 to March Interim Questionnaire 2 March 2009 to February Sleep November 2009 to March May 2014 to May March 2016 to July Interim April 2017 to December * Optional, not all sites completed it. ** Portland Site Only # DXA scans were obtained at Sleep, but not centrally processed *** MIF is not recorded in the database yet. ^ year of follow-up rounded to the nearest half-year updated 7/28/17

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