Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK

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1 Schedule of ccreditation United Kingdom ccreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK ccredited to Unit 16 lacklands Way bingdon usiness Park bingdon Oxfordshire OX14 1DY Contact: Mr Peter Harper Tel: +44 (0) Fax: +44 (0) pharper@cellmark.co.uk Website: Website: Laboratory locations: below s covered by the organisation and their relevant activities details ctivity code ddress Unit 16 lacklands Way bingdon usiness Park bingdon Oxfordshire OX14 1DY Local contact Mr Peter Harper Tel: +44 (0) Fax: +44 (0) pharper@cellmark.co.uk Website: Forensic Testing; Paternity and Relationship testing; Forensic and Veterinary, Forensic nalysis ddress Unit 1 uckshaw Link Ordnance Road uckshaw Village Chorley Lancashire PR7 7EL Local contact Mr Peter Harper Tel: +44(0) Fax: +44(0) pharper@cellmark.co.uk Website: Forensic nalysis ddress The rrow Centre, nnesley Road, Hucknall, Nottinghamshire NG15 8Y Local contact Mr Peter Harper Tel: +44(0) Fax: +44(0) pharper@cellmark.co.uk Website: Forensic nalysis C ssessment Manager: JL1 Page 1 of 22

2 Schedule of ccreditation United Kingdom ccreditation Service ccredited to DETIL OF CCREDITTION Forensic Testing The organisation has demonstrated adherence to the relevant requirements of the Forensic Science Regulators of Practice and Conduct (Version 3.0, February 2016) in relation to their Forensic ctivities TISSUES Forensic nalysis lood - Whole - Stains - FT cards Semen - Whole - zoospermic Saliva - Whole - Stains - FT Cards - Swabs (buccal cells) Hair Cellular Material DN Profiling: Short Tandem Repeat (STR) DN profiling for forensic analysis of: - Low Template DN (elevated cycle number (SGM Plus) and post PCR enhancement SGM Plus and NGM SElect)) - Crime Scene Samples meeting the requirements of the Custodian for the Purpose of Supply to the National DN Database - Subject Samples (PCE and Volunteer) meeting the requirements of the Custodian for the Purpose of Supply to the National DN Database - Elimination Database samples (VED/SED and PED) - Environmental Monitoring Samples using manual/automated extraction - utomate Express (FSP0124) - Qiagen (EZ1) (FSP0050) - Chelex (FSP0038) - FT purification (STR0187/FSP0156) - DN IQ (STR0119) using manual quantification - Pico Green - Oli Green (QC0005/STR0161) Real Time Quantification using Quantiplex HYres Dual RT-PCR (FSP0054) (FSP0156/STR0159) using manual amplification (PCR) and the following chemistry: - SGM Plus - NGM SElect (FSP0028/STR0162/STR0183) using Electrophoresis - pplied iosystems 3100/3130/3130xl Genetic nalyser ssessment Manager: JL1 Page 2 of 22

3 Schedule of ccreditation United Kingdom ccreditation Service ccredited to TISSUES Forensic nalysis lood - Stains Saliva - Stains - Swabs Hair Cellular Material DN Profiling: Short Tandem Repeat (STR) DN profiling for forensic analysis of: - Crime Scene Samples meeting the requirements of the Custodian for the Purpose of Supply to the National DN Database - Environmental Monitoring Samples using manual/automated extraction - Qiagen (EZ1) (FSP0050) using manual quantification - Real Time Quantification using Thermo Fisher 7500 (FSP0156/STR0159) using manual amplification (PCR) and the following chemistry: - NGM SElect (FSP0028/STR0162/STR0183) using Electrophoresis - pplied iosystems 3130 Genetic nalyser C ssessment Manager: JL1 Page 3 of 22

4 Schedule of ccreditation United Kingdom ccreditation Service ccredited to TISSUES (cont d) Forensic nalysis (cont d) Related Opinions and Interpretation Interpretation of DN profiles generated internally from crime stains (single source/major-minor mixtures/complex mixtures) and reference samples Statistical analysis and comparison of DN profiles generated from crime stains with compatible reference DN profiles (internally generated or from other accredited laboratories) Documented In-House methods (STR0147/STR0183/STR0184/STR0 194/FRP0122/FSP0133/GM0004/G M0005/FSP0021) - Genetic Characterisation o GMID o GeneMapper IDX o Expert systems: Mixtures nalyser Sequencher DN Resolve ReliCalc STRMix (v2.3) Forensic Testing lood - FT cards Saliva - FT Cards DN Profiling: Short Tandem Repeat (STR) DN profiling for forensic analysis of: Subject Samples for input into population databases. (STR0202) & manufacturer specifications using FT purification (STR0187/FSP015) and amplification (PCR) using the following chemistry: - PowerPlex Fusion 6C (STR0203) using Electrophoresis: - pplied iosystems 3500 Genetic nalyser Genetic characterisation using documented in-house methods (STR0204): - GeneMapper IDX v1.4 ssessment Manager: JL1 Page 4 of 22

5 Schedule of ccreditation United Kingdom ccreditation Service ccredited to TISSUES (cont d) Forensic nalysis (cont d) lood - Whole - Stains - FT cards Semen - Whole - zoospermic Saliva - Whole - Stains - FT Cards - Swabs (buccal cells) Faeces Urine DN Profiling: Short Tandem Repeat (STR)/Mitochondrial/Y Chromosome DN profiling for forensic analysis of: - Low Template DN (elevated cycle number and post PCR enhancement) - Crime Scene Samples using manual/automated extraction - utomate Express (FSP0124) - Chelex (FSP0038) - FT purification (STR0187/FSP0156) - DN IQ (STR0119) using Manual quantification - Oli Green (QC0005/STR0161) - Real Time Quantification using Quantiplex HYres Dual RT-PCR (FSP0054) Mini-gel Stomach Contents Hair Cellular Material ody Tissue - Nail - Muscle - one - Teeth - mnoitic Fluid - Products of conception using Manual amplification (PCR) and the following chemistry: - SGM Plus (FSP0156/STR0159) - NGM SElect - Identifiler - Yfiler (FSP0156) - mtdn (MIT0004) - Minifiler (FSP0070) - Powerplex Y23 (FSP0149) (FSP0028/STR0162/STR0188) using Electrophoresis - pplied iosystems 3100/3130/3130xl Genetic nalyser ssessment Manager: JL1 Page 5 of 22

6 Schedule of ccreditation United Kingdom ccreditation Service ccredited to TISSUES (cont d) Forensic nalysis (cont d) Related Opinions and Interpretation Interpretation of DN profiles generated internally from crime stains (single source/major-minor mixtures) and reference samples Documented In-House methods (STR0168/STR0183/STR0184/STR0 194/FRP0135) - Genetic Characterisation o GMID o GeneMapper IDX o Expert systems: Sequencher Statistical analysis and comparison of DN profiles generated from crime stains with compatible reference DN profiles (internally generated or from other accredited laboratories) ssessment Manager: JL1 Page 6 of 22

7 Schedule of ccreditation United Kingdom ccreditation Service ccredited to TISSUES (cont d) Relationship nalysis lood - Whole - Serum - Stains Semen - Whole - zoospermic Saliva - Whole - Swabs (buccal cells) Faeces Hair ody Tissue - Nail - Muscle - one - Teeth - mnoitic Fluid - Products of conception Short Tandem Repeat (STR)/Mitochondrial/Y Chromosome DN profiling for relationship testing for: - Paternity - Maternity - Sibling - Familial Searching - Extended relationship (unt/uncle, Niece/Nephew, Grandparent, Grandchild, Cousin) using Manual/utomated extraction - utomate Express (FSP0124) - Chelex (FSP0038) - FT Purification (STR0187) (FSP0054) using Manual quantification Real Time Quantification using Quantiplex HYres Dual RT-PCR (FSP0054) using Manual amplification and the following chemistry: - NGM Select (STR0161) - Yfiler (FSP0065) - Identifiler Direct (STR0187) - Powerplex Fusion (STR0183) - Powerplex Y23 (FSP0149) using Electrophoresis (FSP0028/STR0162/STR0188) - pplied iosystems 3130 Genetic nalyser Related Opinions and Interpretation Comparison, interpretation and statistical analysis of DN profiles against compatible DN Profile information from within submitted cases Documented In-House methods (STR0186/STR0183/STR0184/STR0 194) - Genetic Characterisation o GMID 3.2 o GeneMapper IDX ssessment Manager: JL1 Page 7 of 22

8 Schedule of ccreditation United Kingdom ccreditation Service ccredited to TISSUES (cont d) Forensic nalysis NY MTERIL Searching for: - lood - Semen - Saliva - Urine - Hairs (FSP0027/FSP0069) using: - visual examination - White light - Filter source - High Energy light Sources - low power microscopy - high power microscopy - chemical testing (see below), C, C, C, C Recovery and preparation, including for contingency purposes, for subsequent DN analysis by an ISO/IEC accredited laboratory of the following from searched materials and swabs: - lood - Semen - Saliva - Hairs - Cellular Material (FSL0002) using: - cutting - swabs and swabbing - extraction of stained materials - extraction of swabs - taping - mini-taping, C, C, C, C lood Presumptive testing for blood via detection of: - Peroxidase - Human Haemoglobin (FSL0027, FSL0069, FSL0070, FSL0013, FSL0031, FSL0058, FSL0082) using: - Visual Examination - lternative light sources - KM (Kastle Meyer) - Hemastix ( only (CS DN)) - Luminol - Hydrogen Peroxide - OTI, C,C, C,, Related Opinions and Interpretations Identification, interpretation and recording of blood patterns (P) on clothing and other items examined at the laboratory (FSL0044/TG0010) using: - visual examination - low power microscopy - dimensional. ssessment Manager: JL1 Page 8 of 22

9 Schedule of ccreditation United Kingdom ccreditation Service ccredited to TISSUES (cont d) Forensic nalysis (cont d) Semen Presumptive testing for seminal fluid, via detection of: - cid Phosphatase - Choline (FSL0005, FSL0007) using: - Visual Examination - lternative light sources - cid phosphatase detection (colour reaction) - Choline detection by Florence Iodine test Confirmatory testing for seminal fluid via identification of spermatozoa (FSL0009/FSL0017/FSL0008) using: - High power microscopy - Christmas Tree staining - Haematoxylin and Eosin staining Saliva Presumptive testing for saliva via detection of: - mylase (FSL0004) using: - Visual examination - lternative light sources - Phadebas paper - Phadebas tube test Saliva Presumptive testing for saliva via detection of: - mylase (FSL0004) using: - Phadebas paper - Phadebas tube test C Urine Presumptive testing for urine via detection of: - Urea (FSL0060) using: - Visual examination - lternative light sources - DMC - zostix Hairs Differentiation of human and animal hairs (FSL0010/FSL0068) using: - visual examination - low power microscopy - high power microscopy ssessment Manager: JL1 Page 9 of 22

10 Schedule of ccreditation United Kingdom ccreditation Service ccredited to TISSUES (cont d) Forensic and Veterinary lood ody Tissue DN FT Cards nalysis of ovine Short Tandem Repeat (STR) loci defining extraction, amplification, electrophoresis and profile designation of ovine samples using Finnzymes kit 1.2 (OVSTR0003) Forensic samples and meat products Related Opinions and Intepretations Identification of animal species by sequencing of the 12S rrn mitochondrial gene from DN extracts (SID0004) defining extraction, amplification, sequencing and analysis and comparison with reference sequences and analysis (SID ). In house methods for relationship analysis (OVSTR0006-7) TISSUES - TOXICOLOGY Forensic nalysis Hair Screening for the presence of drugs of abuse: - mphetamines - enzodiazepines - Cocaine - Ketamine - Methadone - Methamphetamine - Mephedrone - Opiates (TOX0009) using liquid-liquid extraction and LC-MSMS Hair Confirmation and quantitation of: (TOX0013) using liquid-liquid extraction and LC-MSMS mphetamine Group : - mphetamine - Methamphetamine - MD - MDM - MDE - MD - Mephedrone ssessment Manager: JL1 Page 10 of 22

11 Schedule of ccreditation United Kingdom ccreditation Service ccredited to TISSUES TOXICOLOGY (cont d) Forensic nalysis (cont d) Hair Confirmation and quantitation of (cont d): Cocaine Group : - Cocaine - Cocaethylene - enzoylecgonine - Norcocaine - nhydroecgonine methyl ester (EME) - Ecgonine methyl ester (EME) (TOX0013) using liquid-liquid extraction and LC-MSMS Opioid Group : - Methadone - EDDP enzodiazepines Group - Diazepam - Temazepam - Oxazepam - Chlordiazepoxide - Desmethyldiazepam Opiate Group : - Morphine - ine - 6-Monoacetylmorphine (6-MM) - Heroin - 6 cetylcodeine - Dihydrocodeine Hair Confirmation and quantitation: - Ketamine (TOX0014) using liquid-liquid extraction and LC-MSMS Confirmation and quantitation: - Ethyl Glucoronide (EtG) (TOX0015) using solid phase extraction and GC-MSMS ssessment Manager: JL1 Page 11 of 22

12 Schedule of ccreditation United Kingdom ccreditation Service ccredited to TISSUES -TOXICOLOGY (cont d) Forensic nalysis (cont d) Hair Confirmation only : Cannabis Group : - Cannabinol - Cannabidiol (TOX0011) using solid-phase extraction and GC-MSMS Hair Confirmation and quantitation: Cannabis Group : - THC - THC-OH - THC-COOH (TOX0011) using solid-phase extraction and GC-MSMS Whole lood (Preserved) Detection and quantitation of drugs in relation to s5 of the Road Traffic ct 1988 (as amended) and The Drug Driving (Specified Limits) (England and Wales) Regulations 2014: Documented in house (TOX0026) using: - Protein Precipitation - LCMS-MS mphetamine (250 ug/l) enzoylecgonine(50 µg/l) Clonazepam (50 µg/l) Cocaine (10 µg/l) Diazepam (550 µg/l) Flunitrazepam (300 µg/l) Ketamine (20 µg/l) Lorazepam (100 µg/l) Lysergic cid Diethylamide LSD (1µg/L) Methadone (500 µg/l) Methylamphetamine (10 µg/l) Methylenedioxymethampheta mine MDM (10 µg/l) 6-Monoacetylmorphine (5 µg/l) Morphine (80 µg/l) Oxazepam (300 µg/l) Temazepam (1000 µg/l) ssessment Manager: JL1 Page 12 of 22

13 Schedule of ccreditation United Kingdom ccreditation Service ccredited to TISSUES - TOXICOLOGY (cont d) Forensic nalysis (cont d) Whole lood (Preserved) (cont d) Delta-9-tetrahydrocannabinol (THC) (2 µg/l) Documented in house (TOX0024) using: - Solid phase extraction - GC-MS-MS Detection and quantification of alcohol in relation to the Road Traffic ct. (10mg/mL) Documented in house (TOX0029) using : - HS-GC-FID, lood (Preserved, Unpreserved) Qualitative and Quantitative nalysis of Drugs of buse mphetamines: - mphetamine - utylone - Methamphetamine - Methylenedioxyamphe tamine (MD) - Methylenedioxymetha mphetamine (MDM) - Methylenedioxypyroval erone (MDPV) - Methylone - Naphyrone Documented in house (TOX0026) using: - Protein Precipitation - LCMS-MS enzodiazepines: - lprazolam - Clonazepam - Diazepam - Desmethyldiazepam - Flunitrazepam - Lorazepam - Oxazepam - Phenazepam - Temazepam Cathinones: - Mephedrone - (4- Methylmethcathinone) ssessment Manager: JL1 Page 13 of 22

14 Schedule of ccreditation United Kingdom ccreditation Service ccredited to TISSUES - TOXICOLOGY (cont d) Forensic nalysis (cont d) lood (Preserved, Unpreserved) (cont d) Qualitative and Quantitative nalysis of Drugs of buse (cont d) Documented in house (TOX0026) using: - Protein Precipitation - LCMS-MS Cocaine: - enzoylecgonine - Cocaethylene - Cocaine - Ecgonine methyl ester (EME) - Norcocaine Opioids: - 6-cetylcodeine - uprenorphine - 6-Monoacetylmorphine (6-MM) - ine - Dihydrocodeine - Morphine - Methadone - 2-ethylidene-1,5- dimethyl-3,3- diphenylpyrrolidine (EDDP) Piperazines: - enzylpiperazine (ZP) - Meta- Chlorophenylpiperazin e (m-cpp) - 3- Trifluoromethylphenyl - piperazine (TFMPP) Ketamine Lysergic cid Diethylamide (LSD) ssessment Manager: JL1 Page 14 of 22

15 Schedule of ccreditation United Kingdom ccreditation Service ccredited to TISSUES - TOXICOLOGY (cont d) Chemical nalysis (cont d) lood (Preserved, Unpreserved) (cont d) Qualitative and Quantitative nalysis of Drugs of buse (cont d) Documented in house (TOX0024) using: - Solid phase extraction - GCMS-MS Cannabinoids: Cannabidiol Cannabinol Carboxy-THC (THC-COOH) Delta-9-tetrahydrocannabinol (THC) Hydroxy-THC (THC-OH) DMGE Forensic nalysis Damage (Clothing and Fabric material) Related Opinions and Interpretations Examination, assessment and evaluation of a damage item, comparison of damage with suspected instrument to determine the likelihood the suspected instrument caused the damage. (FSL0003) using: - visual examination - microscopy - dimensional - Physical fit (FSL0075/FSL0039) FIRES Forensic nalysis Recovery of fibres for contingency purposes from clothing and objects (FSL0010/FSL0055) using - visual examination - low power microscopy - taping - forceps Search and recovery of fibres from clothing and objects for analysis (including tapings) (FSL0010/FSL0055) using - visual examination, - low power microscopy and screening - fibre recovery (taping) - mounting ssessment Manager: JL1 Page 15 of 22

16 Schedule of ccreditation United Kingdom ccreditation Service ccredited to FIRES (cont d) Forensic nalysis (cont d) Identification of fibre type (FRP0085/ FSL0087/FSL0089) using - polarised light microscopy - FTIR - MSP Spectroscopic analysis of fibres in the visible range for the purpose of comparison of fibres (method ref) using - visible Microspectrophotometry Comparison of fibres (FRP0085/ FSL0087/FSL0089) using - stereo microscopy - polarised light microscopy - comparison microscopy - FTIR - MSP Opinion and Interpretation The evaluation of the significance of any matching features between the suspect and reference/control fibre to determine the likelihood of the suspect fibre coming from a specific source ssessment Manager: JL1 Page 16 of 22

17 Schedule of ccreditation United Kingdom ccreditation Service ccredited to FLMMLE LIQUIDS (FIRE CCELERNTS) Forensic nalysis Material Recovered from and associated with Fire Scenes Debris and materials recovered from fires and suspected fire scenes Recovery of potential fire accelerants Documented in-house method using - Direct sampling - Headspace sampling Common fire accelerant liquids Recovery of potential fire accelerants Documented in-house method (CCEL0002) using - Direct sampling - Headspace sampling Examination and analysis of the following flammable liquids - Petrol - Paraffin - Turpentine substitute - White spirit - Diesel - lcohols (methanol/ethanol) (CCEL0003) using - TENX - TD-GCMS Material Recovered from and associated with Fire Scenes (cont d) nalysis and identification of common fire accelerants : - Petrol - Paraffin - Turpentine substitute - White spirit - Diesel - lcohols (methanol/ethanol) - Lighter Fuels (CCEL0003) using - TENX - TD-GCMS Comparison of common fire accelerants (CCEL0004) using - TD-GCMS ssessment Manager: JL1 Page 17 of 22

18 Schedule of ccreditation United Kingdom ccreditation Service ccredited to GLSS Forensic nalysis Search and recovery of glass fragments from clothing and objects (FSL0029/FSL0033) using visual examination and low power microscopy Preparation of glass fragments for SEM nalysis Documented In-House Method (FSL0066) involving stub preparation. Characterisation of glass fragments Documented In-House methods (FSL0035/FSL0036/FSL0037) using - refractive index determination by oil immersion (GRIM 3) - low power and interference microscopy - re-annealing by tube furnace Opinion and Interpretation The evaluation of the significance of any matching features between the suspect and reference/control glass to determine the likelihood of the suspect glass coming from a specific source. - Database GUN SHOT RESIDUE (GSR / FDR) Forensic nalysis ny Material Recovery of in-organic gun shot residues (primer) (FSL0078) using - carbon coated aluminium stubs - Wipes - Swabbing Recovered Material Identification of in-organic gun shot residues (primer) (GSR0002/GSR0010) using SEM/EDX ssessment Manager: JL1 Page 18 of 22

19 Schedule of ccreditation United Kingdom ccreditation Service ccredited to LCHRYMTORS Forensic nalysis Submitted devices and material recovered from clothing Identification of: - Orthochlorobenzalmalonitril e (CS) - lphachloroacetophenone (CN) - Pelargonic acid vanillylamide (PV) - Capsaicin (Pepper Spray) & (Dihydrocapsaicin) (TOX0002) using GC-MS Legal Classification of devices (Firearms ct 1968) and rticle 45 of the Firearms (N.I.) Order 2004) nalysis to determine the nature of the contents of a device, and whether the item fits the description of a Prohibited Weapon ssessment Manager: JL1 Page 19 of 22

20 Schedule of ccreditation United Kingdom ccreditation Service ccredited to MRKS ND IMPRESSIONS Forensic nalysis Footwear Enhancement of footwear marks recovered from scenes (FSL0026/FSL0090) using - lighting techniques - ESL - gel lifting - digital capture photography Production of test marks from suspect footwear (FSL0025) using - casting (dental stone/bio foam) - powders (ristol black/aluminium/light mineral oil/magnetic powder) - digital capture photography Footwear mark (physically or image) ssessment, comparison and evaluation of footwear with scene marks Documented In-House methods (FSL0025) using visual examination, low power microscopy and dimensional s Opinion and Interpretation The evaluation of the significance of any matching features between the suspect and reference/control footwear marks to determine the likelihood of the suspect mark coming from a specific footwear Documented In-House methods using - Personal experience - Database NFRC system for coding of marks Toolmarks Enhancement of toolmarks (FSL0057) using - lighting technique - casting - digital capture/photography Production of test marks from suspect items (FSL0057) using - casting - digital capture/photography ssessment Manager: JL1 Page 20 of 22

21 Schedule of ccreditation United Kingdom ccreditation Service ccredited to MRKS ND IMPRESSIONS (cont d) Forensic nalysis (cont d) Toolmarks Comparison of submitted marks or marks made from suspect items with marks left at scene Documented In-House methods (FSL0057) using visual examination, low power microscopy, comparison microscopy, dimensional s and photography Opinion and Interpretation The evaluation of the significance of any matching features between the suspect and reference/control toolmarks to determine the likelihood of the suspect mark coming from a specific tool Documented In-House methods (FSL0057) using - Personal experience Marks in blood and enhancement of marks in blood from items recovered from crime scenes (FSL0026/FSL0070) using - Sulphosalicylic acid Hydrogen Peroxide - methanol and heat fixing acid black 1 and leuco crystal violet PINT Forensic nalysis Search and recovery of paint and paint fragments from clothing and objects (FSL0029) using - visual examination - low power microscopy Comparison of recovered and control samples (FSL0061/FSL0062/FSL0064) using - spot tests - florescence - high power microscopy ssessment Manager: JL1 Page 21 of 22

22 Schedule of ccreditation United Kingdom ccreditation Service ccredited to MRKS ND IMPRESSIONS (cont d) Forensic nalysis (cont d) Opinion and Interpretation PINT The evaluation of the significance of any matching features between the suspect and reference/control paint samples to determine the likelihood of the suspect paint fragment coming from a specific source. LCOHOL TECHNICL DEFENCE lcohol Technical Defence (in relation to RT) for sample matrix including lood/urine/breath Estimation of alcohol consumption and elimination with respect to validity of drinking patterns: 1) Effect of alleged post accident alcohol consumption on measured breath/body fluids alcohol levels 2) Effect of alleged spiked drink 3) ack calculations of breath/blood/urine alcohol levels to the time of accident or other incident from 20mg% and above s using mathematical calculations END ssessment Manager: JL1 Page 22 of 22