Recent FMD situation and control strategies in China Dr.Shiqi Sun shiqisun21@hotmail.com The national FMD reference laboratory of the People s Republic of China Lanzhou Veterinary Research Institute, CAAS 2011-12-0112
Contents 1Th 1.The recent outbreaks of ffmdi in China Type AsiaⅠ(2005-2009) 2009) Type A (2009-2010) Type O (2010-2011) 2. Strategies for prevention and control in China 3. New technologies/products developed in LVRI 5
1. The recent outbreaks of FMD in China
Serotype Asia1 From 2005 to 2009, total 46 outbreaks in 17 provinces
Serotype Asia1 in 2009 FMDV Asia1 strains collected in 05-08 20 17 Outbreak 15 Province 10 5 10 7 7 8 3 33 8 7 0 0 0 2005 2006 2007 2008 2009 2010 FMDV Asia1 strains collected in 2009
Survey of epidemiology i in 2009 In 2009, serotype Asia1 reappear, 8 outbreaks in 7 provinces. Sequence analysis suggested that all stains isolated from different province are closed to each other. Since June 2009,there was no outbreak of serotype Asia1.
Serotype A There was outbreaks of serotype A in Wuhan Hubei province of China in Jan 2009
Outbreaks of serotype A Date Species Susceptible Cases Location 22/01/2009 Cattle 294 58 Sunwan, Dongxihu, Wuhan, HUBEI 1 12/02/2009 Cattle 440 41 Wusi, Fen Xian, SHANGHAI 2 30/04/2009 Cattle 413 17 Congcong, Wujing, Changzhou, JIANGSU 3 22/05/2009 Cattle 184 12 Xi'nan, Lin'gui, Guilin, GUANGXI 4 Swine 570 0 22/05/2009 Cattle 78 71 Swine 19 19 08/06/2009 Cattle 290 33 25/11/2009 Cattle 1408 321 Haixing chaoyang village, Pan, Liupanshui, GUIZHOU 5 Bingzhou, Bingcheng district, Bingzhou, SHANDONG 6 Fukang City, Fukang City, Changji Prefecture, XINJIANG 7 15/01/2010 Cattle 37 28 Xi'nier town, XINJIANG 02/02/2010 Cattle 44 26 Beicheng county, XINJIANG Sheep 125 0
2009 20102010 Type A in China 2009 n=7 2010 n=2+1
Serotype O From 2010 to present, there are 25 outbreaks in 9 provinces O/Mya-98 and O/PanAsia are dominant serotype. O/Mya 98 n=20 O/PanAsia n=5
Serotype O/Mya 98 At present, Serotype O/Mya-98 is dominant strain to threaten Chinese livestock. Cattle, pigs, sheep can be infected experimentally and by direct contact. Epidemic frequency slowdown from Sep 2010. There are 2 outbreaks in 2011. Analysis of sequence suggested that it is related with strains isolated from southeast Asia (Thailand, Vietnam and Malaysia >97%).
Serotype O/ O/PanAsia It broke out in Guizhou/Tibet /Ningxia in 2011. This stain mainly infect cattle, pigs do not have typical signs. Analysis of epidemiology suggested it came from southeast Asia, such as Vietnam, Cambodia.
Threaten virus strains O/ME-SA/PanAsia-2 A/Iran-05 Asia1 unnamed strain (G VII?)
SA&SEA O A Asia1 O/Mya 98 O/PanAsia A/SEA Asia1
Northeast Asia 2010.4-Japan 2010.4-Korea 2010.4- Mongolia 2010.7-Russia 2011.2-North Korea
A/Iran/05 O/PanAsia-2 Asia1 The middle East Th middle The iddl Asia A i
2.Strategies for prevention and control
The framework of prevention and control system on FMD laws and regulations strategies and measures technique administrative support system finance guarantee
International measures to control FMD compulsive inoculation with vaccine to susceptible animals B slaughter of animals which were infected or suspected infection A measures C Restriction of animal and animal products movement Epidemiological i i l survey, risk analysis and forecast and forewarning E D Strengthening quarantine system
Th P i C t l P th f FMD t l The Progressive Control Pathway for FMD control (PCP-FMD)
Laws and Regulations of China prevention and control norm for foot and mouth disease Animal Epidemic Prevention Law of the People's Republic of China Regulation on the Bio-safety Management of Pathogenic Microbe Labs
Outlines of prevention and control Vaccination Restriction of animal and animal products movement Measures slaughter of infected or suspected infected animals Serological and etiological surveillance. Compulsively inoculated with vaccine combined with slaughtering in China
3. New technologies/products developed in LVRI
The National FMD Reference Laboratory(NFMDRL) Lanzhou veterinary research institute (LVRI)was set up by Ministry of Agriculture in 1958 NFMDRL was established by Ministry of Agriculture in 2002. NFMDRL was assigned one of OIE FMD Reference Laboratory by OIE. 兰州兽医研究所 家畜疫病病原生物学国家重点实验室 世界动物卫生组织参考实验室
P3 biosafe laboratoryy Facilities Excellent group
Groups at NFMDRL Basic research Epidemiology Diagnostic and detection tools development Vaccine development Surveillance technology (Software of System Supervise, Databases of information )
Diagnostic Kit Indirect sandwich ELISA Kit Liquid phase blocking ELISA(LPBE) Kit 3ABC-ELISA Kit Multi-RT-PCR diagnosis Kit RT-PCR diagnosis i Kit Real time quantitative PCR Cllid Colloid-gold ld test t strips
Vaccine Chemically inactivated oil adjuvant vaccines: Type O Type A Type Asia1 monovalent vaccine Type O-A Type O-Asia1 bivalent vaccine Type O-A-Asia1 Asia1 multivalent vaccine
Challenge after immunization of O/MYA98/2010 vaccine Virus strains Pigs (three batch of vaccine) Cattle(two batch of vaccine) PD 50 PD 50 MYA98 10.81,13.59,10.81 7.08,10.81 PanAsia 7.49,10.81,11.84 11.84,9.00 Swine the eligible rate of antibody against serotye O( 1:64) Dose(Pig) 21d (%) 28d(%) Full dose 14/20,70.0%, 17/20,85.0%, 1/3 dose 8/20,38.1% 14/20,70.0% 1/9 dose 1/20,5.0% 3/20,15.0%
Persistent FMDV infection and mucosal immune response Carriers are defined as those ruminants in which FMDV persists (detected) in the pharyngeal region beyond 28 days after infection Carriers are a potential source for the generation of antigenic variants. Transmission of FMDV between animals is primarily via oral-pharyngeal exposure from contaminated feed and aerosols emitted from infected animals. This has led to a particular interest in the local, mucosal immune response to FMDV infection in the pharynx since, following exposure, this region is the most common site for primary virus replication.
Mucosal immunity of FMD vaccine? To reduce the persistent infection, the New technologies have been developed to enhance mucosal immunity of FMD and make the protection against FMDV move up to the oral-pharyngeal,.
Genetic engineering vaccine Reverse genetics based vaccine Parental virus SL1-a SL1-b SL2 Mutation ti SL1 SL2 Engineered viruses 3 UTR 3D L protein RGD P1 region P2-P3 P3 region P1region Aa
a TEM of rfmdv Plaque formation assay suckling mice
Genetic engineering i vaccine Virus-like Particles based vaccine (Eco.li system) dose protection ti (%) PD50 in cattle 100%(16/16) protection ti in pig 1 5/5 6.34 1/3 4/5 1/9 2/5 Ctrl 0/3
Genetic engineering i vaccine DNA vaccine Recombinant adenovirus vectored vaccine
Genetic engineering i vaccine Silkworm-Baculovirus l i vector vaccine Transgenic plant tbased vaccine
ACKNOWLEDGEMENTS LIU Xiangtao (Director of NRL-FMD, LVRI) GUO Huichen (LVRI) ZHENG Hiaxue (LVRI) HE Jijun (LVRI) GUO Jianhong (LVRI) CHEN Juan (LVRI) SHANG Youjun (LVRI) JIN Ye (LVRI) CAI Xuepeng (A-CDC)
Th k you Thank 谢谢大家 Welcome to Lanzhou z Veterinaryy Research Institute