ccredited to Laboratory locations: Schedule of ccreditation United Kingdom ccreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Chester & Wirral Microbiology Service 11 Bassendale Road Wirral CH62 3QL Contact: Mr Iain Green Tel: +44 (0)1244 363354 E-Mail: iain.green@nhs.net below s covered by the organisation their relevant activities details ctivity code ddress Chester & Wirral Microbiology Service 11 Bassendale Road Wirral CH62 3QL Local contact Mr Iain Green See above Full Microbiology diagnostic service including: Bacteriology Mycology Parasitology Serology Molecular testing Site activities performed away from the locations listed above: details ctivity code On Call Laboratory Pathology rrowe Park Hospital rrowe Park Road Wirral CH49 5PE Mr Iain Green Microscopy culture of : CSF Joint fluids Theatre pus fluids s < 3 yrs age B On Call Laboratory Pathology Countess of Chester Hospital Liverpool Road Chester CH2 1UL Mr Iain Green Microscopy culture of : CSF Joint fluids Theatre pus fluids s < 3 yrs age C ssessment Manager: KS 1 of 8
Schedule of ccreditation United Kingdom ccreditation Service ccredited to DETIL OF CCREDITTION HUMN TISSUES ND FLUIDS clinical diagnosis Routine samples: spirates Isolation of microorganisms of clinical significance culture, B, C Fluids, B, C Pus MBSOP 21, B, C Samples from sterile sites MBSOP 18, B, C MBSOP 29, B, C Bone Cellular fluid material contained on swabs, catheter tips intrauterine contraceptive devices Genital samples Semen Tissues Cellular fluid material contained on swabs Screening for Methicillin- Resistant Staphylococcus aureus (MRS) culture MBSOP 44 Rectal swab, Screening for Resistant organisms VRE CPE culture MBSOP 46 Blood Joint fluids Isolation of microorganisms of clinical significance Liquid media culture MBSOP 52 MESOP 12 Biomérieux BacTalert ssessment Manager: KS Page 2 of 8
Schedule of ccreditation United Kingdom ccreditation Service ccredited to HUMN TISSUE ND Cerebrospinal fluid (CSF) Detection, identification quantification (inc. differential) of: White blood cells Red blood cells Bacterial cells Microscopy & staining (Gram differential) MBSOP 21 MBSOP 11, B, C CSF Isolation of microorganisms of clinical significance culture MBSOP 21 MBSOP 11, B, C Bronchoalveolar lavage Bronchial aspirate, brushings & washings Cellular fluid material contained on swabs Endotrachael tube specimens Nasopharyngeal aspirate Pleural fluid Sputum Isolation of microorganisms of clinical significance Detection, identification quantification of: culture MBSOP 42 Staining (inc. Gram) microscopic examination spirates White blood cells MBSOP 21, B, C Fluids Red blood cells MBSOP11, B, C CPD Bacterial cells MBSOP52 Corneal scrapes Yeast cells fungal hyphae MBSOP42 Eye (specimens from) Tissues BL ssessment Manager: KS Page 3 of 8
Schedule of ccreditation United Kingdom ccreditation Service ccredited to HUMN TISSUE ND Detection, identification quantification of: White blood cells Red blood cells Squamous epithelial cells Bacterial cells Casts Yeasts utomated urinalysis, manual examination MBSOP 33 UF1000 nalyser- MESOP 36 /or Manual microscopic examination KOV slides, B, C Isolation of microorganisms of clinical significance culture MBSOP 33, B, C Isolation of bacterial pathogens: Campylobacter spp. Escherichia coli O157 Salmonella sp. Shigella spp. Vibrio spp. Yersinia spp. Pleisiomonas spp. culture MBSOP59 Hair Nail Skin Isolation identification of clinically significant fungi Manual inoculation, media culture, macroscopic & microscopic examination MBSOP 38 ssessment Manager: KS Page 4 of 8
Schedule of ccreditation United Kingdom ccreditation Service ccredited to HUMN TISSUE ND Cultures isolated in-house from all samples listed above Identification of microorganisms of clinical significance Manual /or automated biochemical methods Vitek 2- MESOP 24 Matrix-assisted laser desorption/ionisation MESOP 32 Vitek MS Maldi-ToF Bacterial cultures isolated inhouse from all samples listed above ntimicrobial susceptibility testing of clinically significant bacteria Manual /or automated antimicrobial susceptibility testing using the latest CLSI methodology Vitek 2 using in-house procedure MESOP 24 Disc diffusion & gradient minimum inhibitory concentration determination using in-house procedure MBSOP 06 Female genital tract swabs Microscopic diagnosis of bacterial vaginosis Gram stained microscopic examination MBSOP 29 utomated staining using Prolab Polystainer as per MESOP39 Detection of ova, cysts parasites (OCP) Unstained iodine stained direct microscopic examination /or Formol-ether faecal concentration microscopic examination MBSOP 59 ssessment Manager: KS Page 5 of 8
Schedule of ccreditation United Kingdom ccreditation Service ccredited to HUMN TISSUE ND FLUIDS Detection of Cryptosporidium oocysts uramine fluorescent microscopic examination MBSOP 59 Detection of Schistosoma haematobium ova Microscopic examination using inhouse procedure: MBSOP 33 Nasopharyngeal aspirate Swabs Culture isolate suspension Detection or exclusion of: Influenza Influenza B Respiratory syncytial virus MRS CPE VRE Norovirus Difficile Toxin Genes tcdb, cdt tcdc utomated Molecular Testing (PCR) MBSOP 73 MBSOP 49 MBSOP 71 MBSOP 72 MVSOP 14 MBSOP 70 : Cepheid GeneXpert Detection of Legionella pneumophilia serotype 1 antigen Streptococcus pneumoniae antigen Immuno-chromatographic assay MVSOP 12 Launch TRU ntigen kit C.difficile toxin B GDH Immuno-chromatographic assay TekLab ToxB Quikchek EI Dynex DS2 Premier GDH MBSOP 70 MESOP 29 Rotavirus adenovirus Immuno-chromatographic assay Coris Combi-strip MVSOP 13 ssessment Manager: KS Page 6 of 8
Schedule of ccreditation United Kingdom ccreditation Service ccredited to HUMN TISSUES ND Serological examination clinical diagnosis Helicobacter ntigen EI Dynex DS2 Premier Platinum HpS Plus MBSOP 09 MESOP 29 Microbial serology for: Enzyme Immuno-ssay (EI) Roche Cobas 6000 MESOP 30 Hepatitis B surface antibody MVSOP 29 CMV IgM IgG MVSOP 34 Hepatitis IgG IgM MVSOP 04 Hepatitis B surface antigen MVSOP 02 Hepatitis C antibody MVSOP 28 HIV ag ab MVSOP 05 HTLV ab MVSOP 27 Rubella IgG IgM MVSOP 07 Syphilis IgG MVSOP 15 Toxoplasma IgG MVSOP 09 Microbial serology for: EI - Dynex BEST 2000 MESOP 09 Chlamydia IgG MVSOP 32 EBV IgM IgG MVSOP 25 Measles IgG MVSOP 30 Varicella Zoster IgG MVSOP 06 Mycoplasma IgM MVSOP 10 Microbial serology for: Parvovirus IgM IgG EI - Organotec legria DOC1613 ssessment Manager: KS Page 7 of 8
Schedule of ccreditation United Kingdom ccreditation Service ccredited to HUMN TISSUES ND Serological examination Microbial serology for: EI - VIDS DOC1619 Hepatitis B Markers MVSOP 02 Varicella IgG MVSOP 06 Hepatitis B Core IgM IgG MVSOP 02 Lymes (Borrelia) IgM IgG MVSOP 24 HIV antigen-antibody Immuno-chromatographic strip method lere HIV-combo kit MVSOP 05 Syphilis antibody Fujirebio Espline MVSOP 15 Nasopharyngeal aspirate RSV antigen detection Immuno-chromatographic strip method MVSOP08 Binax NOW kit END ssessment Manager: KS Page 8 of 8