Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK
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1 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Issue No: 001 Issue date: 08 June 2017 Dept of Microbiology Great Western Hospitals NHS Foundation Trust The Great Western Hospital Marlborough Road Swindon SN3 6BB Contact: Emma Hallett Tel: +44 (0) Fax: +44 (0) Website: Testing performed at the above address only DETAIL OF ACCREDITATION AND TISSUES Diverse corporal specimens Diverse corporal specimens clinical diagnosis Detection of white blood cells, epithelial cells, gram positive and negative bacteria (semiquantitative) General isolation and characterisation of aerobic, micro-organisms MIC-S-042 by gram stain and microscopy Manual inoculation and culture using procedures: MIC-S-001 Intraocular fluids and corneal scrapes MIC-S-002 Peritoneal dialysis fluid MIC-S-004 Antral washouts MIC-S-006 Blood transfusion reactions MIC-S-008 Tip specimens MIC-S-009 Cerebrospinal fluid MIC-S-010 Eye swabs and canalicular pus MIC-S-011 Faeces specimens MIC-S-013 Female genital swabs MIC-S-023 GU clinic specimens MIC-S-024 MRSA screening specimens MIC-S-025 Fluid and bile specimens Assessment Manager: HL Page 1 of 9
2 Diverse corporal specimens Aerobic, microaerophilic and anaerobic microorganisms isolated inhouse General isolation and characterisation of aerobic, micro-organisms Identification of aerobic, micro-organisms Manual inoculation and culture using procedures: MIC-S-026 Sputum and BAL MIC-S-028 Urine specimens MIC-S-030 Wound specimens MIC-S-064 Tissue specimens MIC-S-151 Cough swab specimens MIC-S-152 Contact lens case solution MIC-S-164 Respiratory specimens MIC-S-185 Genital specimens (excluding female genital swabs) MIC-E-019 using BioMerieux VITEK 2 (Automated Identification (ID) and Antimicrobial susceptibility testing (AST) instrument) MIC-S-034 BBL crystal ID software MIC-S-038 Identification of Vibrio sp MIC-S-044 Identification of Neisseria sp MIC-S-050 Identification of Corynebacterium sp MIC-S-052 Enteric slide serology MIC-S-061 Kohn s media MIC-S-176 Identification of Bordetella pertussis and Bordertella parapertussis MIC-S-177 Identification of Legionella species Assessment Manager: HL Page 2 of 9
3 Aerobic, microaerophilic and anaerobic microorganisms isolated inhouse Identification of aerobic, micro-organisms MIC-E-019 using BioMerieux VITEK 2 (Automated Identification (ID) and Antimicrobial susceptibility testing (AST) instrument) MIC-S-178 Miscellaneous Tests for Bacterial Identification MIC-S-179 Identification of Yersinia species from faeces MIC-S-182 Identification of Moraxella species and morphologically similar bacteria MIC-S-183 Identification of Pasteurella species and morphologically similar bacteria MIC-S-184 Identification of Haemophilus species and the HACEK group of organisms MIC-S-195 Introduction to the ID of medically important bacteria MIC-S-196 Identification of aerobic actinomycetes MIC-S-197 Identification of anaerobic cocci MIC-S-198 Identification of anaerobic gramnegative rods MIC-S-199 Identification of anaerobic atinomycetes species MIC-S-200 Identification of clostridium species MIC-S-201 Identification of enterobacteriaceae MIC-S-202 Identification of glucose nonfermenting gram-negative rods Assessment Manager: HL Page 3 of 9
4 Aerobic, microaerophilic and anaerobic microorganisms isolated inhouse Whole blood Identification of aerobic, micro-organisms Antimicrobial susceptibilities of aerobic, microaerophilic and anaerobic micro-organisms General isolation of microorganisms of clinical significance MIC-E-019 using BioMerieux VITEK 2 (Automated Identification (ID) and Antimicrobial susceptibility testing (AST) instrument) MIC-S-203 Identification of shigella species MIC-S-204 Identification of escherichia coli 0157 MIC-S-205 Identification of salmonella species MIC-S-206 Identification of campylobacter species MIC-E-019 using BioMerieux VITEK 2 (Automated Identification (ID) and Antimicrobial susceptibility testing (AST) instrument) MIC-S-036 Detection of beta-lactamase enzymes MIC-S-110 manual sensitivity testing MIC-S-116 Detection of ESBL organisms MIC-S-153 GRE screening specimens MIC-S-217 Detection of carbapenamase hydrolysing β-lactamases Automated culture using MIC-S-021 using BioMerieux BacT/ALERT 3D (automated system detecting CO2 production) Stool Stool analysis consistency/appearance MIC-S-011 by macroscopic assessment Stool Glutamate dehydrogenase (GDH) detection MIC-S-175 using C.difficile GDH Enzyme Immunoassay (EIA) Assessment Manager: HL Page 4 of 9
5 Stool Cervicovaginal specimen Urine Urine Urine & CSF Respiratory samples Clostridium difficile toxin A and B detection PCR ribotyping of Clostridium difficile Rotavirus antigen detection Presence of white blood cells, red blood cells, epithelial cells and yeasts (semi-quantitative) Presence of white blood cells, red blood cells and epithelial cells, bacteria and casts (semiquantitative) Presence of white blood cells, red blood cells, epithelial cells, bacteria and casts (semiquantitative) Presence of Schistosoma haematobium Detection of Legionella pneumophila serotype 1 antigen Detection of pneumococcal soluble Antigen Detection of RSV fusion protein antigen MIC-S-175 using VIDAS C.difficile Toxin A & B Enzyme immunoassay (EIA) and Biomerieux minividas (automated Enzyme-Linked Fluorescent Immunoassay (ELFA)) MIC-S-175 using Xpert C.difficile and Cepheid GeneXpert (automated nucleic acid amplification) MIC-S-011 using immunocard STAT Rotavirus Enzyme immunoassay (EIA) MIC-S-013 by microscopy MIC-S-028 using BioMerieux UF1000i (digital microscopy analysis) MIC-S-028 by microscopy MIC-S-020 by microscopy Binax Now kit by Alere MIC-S-132 Binax Now kit by Alere MIS-S-132 Binax Now kit by Alere MIS-S-129 Assessment Manager: HL Page 5 of 9
6 Bacterial cultures isolated in-house from all samples listed above CSF CSF CSF and dialysate fluid Oral and throat swabs Stool Faeces Clear adhesive tape from perianal region Genital Antimicrobial susceptibility testing of clinically significant bacteria Presence of white blood cells and red blood cells (quantitative) Detection of Cryptococcus neoformans capsules Differential of white blood cells Presence of fusospirochaetal bacteria and white blood cells Detection of Cyclospora sp, Isospora sp and Cryptosporidium sp oocysts Presence and identification of ova and parasites Detection of ova, cysts and parasites (OCP) Presence of Enterobius vermicularis ova Detection of Trichomonas vaginalis Manual and/or automated antimicrobial susceptibility testing using the latest British Society for Antimicrobial Susceptibility Testing (BSAC) methodology Disc diffusion & gradient minimum inhibitory concentration using M.I.C.E. and E.tests MIC-S-110. MIC-S-009 by microscopy MIC-S-009 using India Ink and microscopy MIC-S-186 using Diff-Quik stain and microscopy MIC-S-111 using dilute Carbol Fuschin and microscopy MIC-S-053 by Modified Ziehl Neelsen stain and microscopy MIC-S-011 and MIC-S-041 by microscopy Concentration and light microscopic examination using Parasep method: SOP MIC-S-041. MIC-S-011 by microscopy MIC-S-013 by microscopy Assessment Manager: HL Page 6 of 9
7 Serum/plasma Serum/Plasma Serology examination activities for the purpose of clinical diagnosis Microbial serology for: HIV-1 and 2 antibodies plus p24 antigen Hepatitis A IgM Hepatitis B surface antigen Hepatitis B core IgG & IgMHepatitis B surface antibody (quantitative) Hepatitis C antibody Rubella IgG Treponema pallidum Toxoplasma IgG CMV IgG & IGM Epstein-Barr Virus (EBV) Viral Capsid Antigen (VCA) IgM antibody Epstein-Barr Virus (EBV) Viral Capsid Antigen (VCA) IgG antibody Epstein-Barr Nuclear Antigen (EBNA) IgG antibody Infectious Disease in Pregnancy (IDP) screening: Detection of Rubella IgG antibody Documented in-house methods and manufacturer s operating instructions to support clinical diagnosis MIC-E-025 using Abbott Architect (automated random and continuous access chemiluminescent microparticle assay (CMIA) MIC-S-158 using bioelisa Rubella IgG colour and Dynex DS2 (automated microplate Enzyme immunoassay (EIA) Assessment Manager: HL Page 7 of 9
8 Serum/plasma Serum Serology examination activities for the purpose of clinical diagnosis Infectious Disease in Pregnancy (IDP) screening: Detection of Hepatitis B surface antigen Infectious Disease in Pregnancy (IDP) screening: Detection of HIV-1 and 2 antibodies and HIV p24 antigen Infectious Disease in Pregnancy (IDP) screening: Detection of Treponema pallidum antibody Detection of Lymes IgG antibody Detection of Lymes IgM antibody Detection of Helicobacter pylori IgG antibody Detection of Varizella Zoster virus IgG antibody Detection of Measles IgG antibody Documented in-house methods and manufacturer s operating instructions to support clinical diagnosis MIC-S-158 using bioelisa HBsAg 3.0 and Dynex DS2 (automated microplate Enzyme immunoassay (EIA)) MIC-S-162 using Genscreen ULTRA HIV Ag- Ab and Dynex DS2 (automated microplate Enzyme immunoassay (EIA)) MIC-S-191 using bioelisa SYPHILIS 3.0 and Dynex DS2 (automated microplate Enzyme immunoassay (EIA)) MIC-S-189 using VIDAS Lyme IgG and Biomerieux minividas (semi-automated (ELFA)) MIC-S-189 using VIDAS Lyme IgM and Biomerieux minividas (semi-automated (ELFA)) MIC-S-126 using Premier H.pylori Enzyme immunoassay (EIA) and Dynex DS2 (automated microplate Enzyme immunoassay (EIA)) MIC-S-191 using VIDAS Varicella Zoster IgG and Biomerieux minividas (semi-automated (ELFA) MIC-S-191 using VIDAS Measles IgG and Biomerieux minividas (semi-automated (ELFA)) Assessment Manager: HL Page 8 of 9
9 Serum Stool Diverse corporal specimens Staphylococccus isolated from organisms in-house Female Cervicovaginal specimens and male and female urine specimens Female Cervicovaginal specimens and male and female urine specimens Serology examination activities for the purpose of clinical diagnosis Detection of Mumps IgG antibody Molecular examination activities for the purpose of clinical diagnosis Detection of Norovirus nucleic acid Detection of Herpes simplex virus (HSV) type 1 (HSV-1) and HSV type 2 (HSV-2) nucleic acid Detection of Panton-Valentine Leukocidin (PVL) toxin nucleic acid Detection of Chlamydia trachomatis and Neisseria gonorrhoea nucleic acid Detection of Neisseria gonorrhoea nucleic acid Miscellaneous examination clinical diagnosis Documented in-house methods and manufacturer s operating instructions to support clinical diagnosis MIC-S-191 using VIDAS Mumps IgG and Biomerieux minividas (semi-automated (ELFA) MIC-S-171 using Seegene SeePrep (automated robotic system for magnetic bead Nucleic Acid extraction) and Cepheid SmartCycler (automated integrated DNA/RNA amplification and detection). MIC-S-212 using Cepheid SmartCycler (automated integrated DNA/RNA amplification and detection) MIC-S-219 using Seegene SeePrep (automated robotic system for magnetic bead Nucleic Acid extraction) and Cepheid SmartCycler (automated integrated DNA/RNA amplification and detection) MIC-S-174 using Roche Cobas 4800 (automated nucleic acid amplification) MIC-S-213 using Cepheid GeneXpert (automated nucleic acid amplification) Joint fluids Detection of crystals MIC-S-025 by microscopy END Assessment Manager: HL Page 9 of 9
Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK
2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Luton and Dunstable University Hospital NHS Foundation Trust Lewsey Road Luton Bedfordshire LU4 0DZ Contact: Pauline Philip Tel: +44 (0)1582
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Schedule of ccreditation United Kingdom ccreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Issue No: 001 Issue date: 11 ugust 2017 ccredited to Department of Microbiology
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