Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK

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1 Department of Microbiology St Helier Hospital Wrythe Lane Carshalton SM5 1AA Contact: Girish Patel Tel: +44 (0) Testing performed at the above address only DETAIL OF ACCREDITATION Routine samples: Aspirates Cellular and fluid material contained on swabs, catheter tips and intrauterine contraceptive devices Fluids Genital samples Pus Samples from sterile sites Semen Tissues Cellular and fluid material contained on swabs Blood diagnosis Screening for Methicillin-Resistant Staphylococcus aureus (MRSA) following current Public Health England (PHE) Standards for Microbiological Investigations (SMIs) Manual inoculation of culture media MU.SOP.028 MU.SOP.015 MU.SOP.006 MU.SOP.011 MU.SOP.012 MU.SOP.054 MU.SOP.001 MU.SOP.029 MU.SOP.007 MU.SOP.008 MU.SOP.010 Manual inoculation culture media as above and MU.SOP.022 and 043 Liquid media culture MU.SOP and 106 and BacTalert automated microbial detection system Assessment Manager: KS Page 1 of 8

2 Sterile site samples: Tissue Swabs Fluid Bone CAPD Cerebrospinal fluid (CSF) CSF Bronchoalveolar lavage Bronchial aspirate, brushings & washings Cellular and fluid material contained on swabs Endotrachael tube specimens Nasopharyngeal aspirate Pleural fluid Sputum Ascitic Fluids diagnosis Detection, identification and quantification (inc. differential) of: White blood cells Red blood cells Bacterial cells Detection, identification and quantification of: White blood cells Red blood cells Bacterial cells Yeast cells and fungal hyphae Manual media culture MU.SOP004 MU.SOP.019 MU.SOP.004 MU.SOP.018 Microscopy & staining (Gram Stain and differential) using KOVA slides MU.SOP.020 Manual inoculation and media culture MU.SOP.020 Manual inoculation and media culture MU.SOP.033 MU.SOP.034 Staining (inc. Gram) and microscopic examination MU.SOP.037 and MU.SOP.019 Assessment Manager: KS Page 2 of 8

3 Faeces Cultures isolated in-house from all samples listed above diagnosis Detection, identification and quantification of: White blood cells Red blood cells Squamous epithelial cells Bacterial cells Casts Isolation, identification and antimicrobial susceptibility of bacterial pathogens: Campylobacter spp. Clostridium difficile Escherichia coli O157 Salmonella sp. Shigella spp. Vibrio spp. Yersinia spp. Identification of microorganisms of Automated urinalysis, image capture and recognition MU.SOP.003 and Menarini Sedimax and/or Manual microscopic examination MU.SOP.028 Manual inoculation and media culture MU.SOP.023 Manual and/or automated biochemical methods Vitek 2 and Vitek MS Maldi-Tof Matrix-assisted laser desorption/ionisation MU.SOP.049 MU.SOP.106 Assessment Manager: KS Page 3 of 8

4 Bacterial cultures isolated in-house from all samples listed above Faeces Skin Hair Nails Tissue diagnosis Antimicrobial susceptibility testing of clinically significant bacteria Detection of ova, cysts and parasites (OCP) Isolation of clinically significant yeasts and fungi Manual antimicrobial susceptibility testing using the latest EUCAST methodology and Vitek 2: Vitek 2 Disc diffusion & gradient minimum inhibitory concentration determination using in-house procedures Unstained and iodine stained direct microscopic examination and/or Formol-ether faecal concentration and microscopic examination using PARASEP concentrators MU.SOP.024 MU.SOP.049 and MU.SOP.120 And light microscopy Joint fluid samples Examination for crystals Polarised Light microscopy using : MU.SOP.099 Sputum Bronchial Lavage Pleural Fluid Pus or swabs Tissue, lymph nodes or biopsies CSF Isolation and presumptive identification of Acid-alcohol fast bacilli (inc. Mycobacterium spp.) Auramine phenol staining and microscopy confirmation using Kinyouan stain MU.SOP.037 Assessment Manager: KS Page 4 of 8

5 Sputum Bronchial Lavage Pleural Fluid Pus or swabs Tissue, lymph nodes or biopsies CSF diagnosis Detection and isolation of Mycobacterium species Detection of Legionella pneumophilia serotype 1 antigen and pneumococcal antigen BacTAlert automated microbial detection system and fluorescent light microscopy (auramine stain) and light microscopy (Kinyouan stain). Manual culture using Pyruvate LJ and plain LJ media MU.SOP.027 Membrane immunochromatographic assay using Binax kit: MU.SOP.076 and MU.SOP.077 Faeces Rotavirus MU.SOP.038 Immunochromatographic and Enzyme linked fluroscent assay - ELFA measurement principles using Biomerieux VIDAS: Faeces C.difficile toxin and GDH MU.SOP.089 And GDH EIA kit Enzyme immunoassay -EIA measurement using membrane immunoassay, Proflow Prolab diagnostics kit Faeces Norovirus MU.SOP.089 Using EIA Oxoid amplified IDEIA Hp star kit Faeces Helicobacter pylori antigen MU.SOP.083 Assessment Manager: KS Page 5 of 8

6 Serological examination activities for the purpose of clinical diagnosis Serum/Plasma unless otherwise Hepatitis A IgG ab MU.SOP.087 stated Hepatitis A IgM ab HBsAg ab Hepatitis B Total Antibody HBsAb Abbott Architect using Chemiluminescent microparticle immunoassay- CMIA technology: Serum/Plasma/Dried blood spot HCV ab MU.SOP.088 Hepatitis C Antigen HIV Ag/Ab HTLV I/II Ab Rubella IgG ab Syphilis IgG ab Syphilis IgM ab Biomerieux Vidas using Enzyme linked fluorescent assay- ELFA technology: Serum CMV IgG ab MU.SOP.089 CMV IgM ab Hepatitis B core IgM ab HBeAb HBeAg HbsAg HCV Ab HIV ag/ab Lyme IgG ab Lyme IgMab Measles IgGab Rubella IgMab Toxoplasma IgMab VZV IgMab Complement fixation test using Virion serion reagents Serum Detection of total antibody to: MU.SOP.075 Influenza A ab Influenza B ab C.burnetti p.2 ab RSV ab Adenovirus ab C.burnetti p.1ab HSV ab CMV ab Leptospira sp.ab Assessment Manager: KS Page 6 of 8

7 Serological examination activities for the purpose of clinical diagnosis Enzyme immunoassay- EIA technology using Various kits detailed below: Serum unless otherwise stated Enterovirus IgM ab Virion Serion ELISA classic MU.SOP.065 EBV VCA IgG ab EBNA IgG ab EBV VCA IgMab Enzyme immunoassay- EIA Trinity Biotech kits: MU.SOP.066 MU.SOP.067 MU.SOP.068 Heterophile antibody Biokit Monogen Latex kit VSOP 15 Measles IgGab Mumps IgGab Parvovirus IgGab VZ IgG ab VZ IgM ab Measles IgM ab Mumps IgM ab Parvovirus IgM ab Mycoplasma pneumoniae abs Treponema pallidium abs Anti streptolysin O Rubella IgG ab Reagin Abs Enzyme immunoassay- EIA Trinity Biotech kits: MU.SOP.078 MU.SOP.080 MU.SOP.061 MU.SOP.063 MU.SOP.064 Virion Serion Elisa Classic EIA kit MU.SOP.079 MU.SOP.081 MU.SOP.060 Seriodia- MYCO II- Fujirebio Inc using microparticle agglutination MU.SOP.074 Syphagen TPHA Biokit kit using Haemagglutination: MU.SOP.073 Biokit Rheumajet ASO using latex agglutination: MU.SOP.070 Biokit Rubagen kit: MU.SOP.071 Biokit RPR reditest using agglutination: MU.SOP.072 Assessment Manager: KS Page 7 of 8

8 Serological examination activities for the purpose of clinical diagnosis Alere Binax now kit using immunoassay NPA samples RSV antigen MU.SOP.084 Molecular diagnostic examination diagnosis Extraction, PCR amplification and detection Molecular diagnostics for: Real time Polymerase Chain Reaction (PCR) Abbott M2000: Detection of genetic material from: Genital swabs/ Chlamydia trachomatis MU.SOP.091 Neisseria Gonorrhoeae Plasma HIV Viral loads MU.SOP.090 Hain GenoXtract and Fluorocycler using real time PCR: Lesion Swabs HSV 1 and HSV 2 MU.SOP.002 Respiratory Samples Influenza A ( matrix, 2009 H1N1, H1 and H3) Influenza B RSV Corona virus (229E, HKU1, NL63, OC43) Para influenza (1-4) Entero/Rhinovirus Metapneumovirus Adenovirus Boca virus Mycoplasma pneumoniae Legionella pneumophila Chlamydophila pneumoniae Luminex Magpix using RPP assay and Multiplex reverse transcription PCR MU.SOP.094 END Assessment Manager: KS Page 8 of 8