2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Microbiology Contact: Paul Randell St Richards Hospital Tel: +44 (0)1243 831 632 Spitalfield Lane E-Mail: paul.randell@wsht.nhs.uk Chichester Website: www.westernsussexhospitals.nhs.uk PO19 6SE Testing performed at the above address only Routine samples: Aspirates Cellular and fluid material contained on swabs, catheter tips and intrauterine contraceptive devices Fluids Genital samples Pus Samples from sterile sites Semen Tissues Cellular and fluid material contained on swabs Blood DETAIL OF ACCREDITATION diagnosis Microbiology Screening for Methicillin-Resistant Staphylococcus aureus (MRSA) following current Public Health England (PHE) Standards for Microbiological Investigations (SMIs) Manual inoculation, incubation and manual reading of culture media [LP-MIC-GENSWABS] [LP-MIC-GENPUS] [LP-MIC-GU] Manual inoculation, incubation and manual reading of chromogenic or culture media [LP-MIC-MRSA] Liquid media culture using SOP: [LP-MIC-BLOOD] and Biomerieux BacTAlert Assessment Manager: PW Page 1 of 8
Sterile site samples: Tissue Swabs Fluid Bone Cerebrospinal fluid (CSF) CSF Bronchoalveolar lavage Bronchial aspirate, brushings & washings Cellular and fluid material contained on swabs Endotrachael tube specimens Nasopharyngeal aspirate Pleural fluid Sputum Aspirates Eye (specimens from) Fluids Tissues diagnosis Detection, identification and quantification (inc. differential) of: White blood cells Red blood cells Bacterial cells Detection, identification and quantification of: White blood cells (including differential count on ascetic and peritoneal fluids) Red blood cells Bacterial cells Yeast cells and fungal hyphae Manual or automated inoculation, incubation and manual reading of culture media [LP-MIC-GENSTERILSANDTISS] Staining (Gram stain and Methylene Blue differential stain) and microscopic examination using SOPs: [LP-MIC-STAIN] Manual inoculation and media culture using SOP: [LP-MIC-CSF] Manual inoculation and media culture [LP-MIC-RESP] Staining (Gram stain and Methylene Blue differential stain) and microscopic examination using SOPs: [LP-MIC-STAIN] Assessment Manager: PW Page 2 of 8
Faeces Cultures isolated in-house from all samples listed above diagnosis Detection, identification and quantification of: White blood cells Red blood cells Squamous epithelial cells Bacterial cells Casts Isolation, identification and antimicrobial susceptibility of bacterial pathogens: Campylobacter spp. Clostridium difficile Escherichia coli O157 Salmonella sp. Shigella spp. Vibrio spp. Yersinia spp. Pleisiomonas spp. Identification of microorganisms of and their antimicrobial sensitivity using EUCAST methodology Automated urinalysis, image capture and recognition using SOP : [LP-MIC-URINE] and A.Menarini Diagnostics SediMAX system and Manual microscopic examination using SOPs : [LP-MIC-URINE] Manual inoculation and media culture using SOP: [LP-MIC-ENTCULTURE] Manual and/or automated biochemical and non-biochemical methods [LP-MIC-BACID] [WI-MIC-V2-2] [LP-MIC-VITEKMS] [LP-MIC-ANTIBIOTIC] and using the Biomerieux VITEK MS Giles Scientific BIOMIC Assessment Manager: PW Page 3 of 8
Female genital tract swabs diagnosis Microscopic diagnosis of bacterial vaginosis Staining (Gram stain) and microscopic examination using SOPs: [LP-MIC-STAIN] [LP-MIC-GU] Hair Nail Skin Faeces Faeces Mycology Isolation and identification of clinically significant dermatophyte fungi Parasitology Detection of ova, cysts and parasites (OCP) Detection of Cryptosporidium oocysts (confirmation of EIA positives) Detection of Schistosoma haematobium ova Manual inoculation, media culture, and macroscopic & microscopic examination (stained & unstained) using SOPs : [LP-MIC-MYCOL] Unstained and iodine stained direct microscopic examination and/or Formol-ether faecal concentration and microscopic examination [LP-MIC-PARASITE] Cold Ziehl-Neelsen incident-light microscopic examination [LP-MIC-PARASITE] Microscopic examination using inhouse SOPs: [LP-MIC-SCHIST] Assessment Manager: PW Page 4 of 8
Sellotape slide Perianal swab Bile Duodenal aspirates Jejunal aspirates Corneal scrapes Contact lenses Aspirates Cellular and fluid material contained on swabs Fluids Sputum Tissue Nasopharyngeal aspirate diagnosis Detection of Enterobious vermicularis larvae and ova Detection of OCP including: Strongyloides stercoralis larvae, Giardia lamblia trophozoites Cryptosporidium oocysts Cyclospora cayetanesis oocysts Cystoisospora belli oocysts Detection of Acanthamoeba Isolation and presumptive identification of Acid-alcohol fast bacilli (inc. Mycobacterium spp.) Detection or exclusion of Respiratory syncytial virus Microscopic examination [LP-MIC-PARASITE] Microscopic examination (unstained and stained auramine phenol and Cold Ziehl-Neelsen) Using SOPs: [LP-MIC-PARASITE] Microscopic examination of culture plates using SOP: [LP-MIC-ACANTH] Liquid culture, auramine phenol staining and incident-light fluorescence microscopy [LP-MIC-MYCOB] and Biomerieux MB/BacT Membrane Immunochromatographic assay using SOP: [LP-MIC-RSVNPAS] and: Alere BinaxNOW RSV Assessment Manager: PW Page 5 of 8
diagnosis Detection of Legionella pneumophilia serotype 1 antigen Membrane immunochromatographic assay using SOP: [LP-MIC-LEGAG] and BinaxNOW Legionella urinary antigen test kit Faeces Detection of Rotavirus Immunogold-based horizontal-flow membrane assay using SOP: Serology Serological examination activities for the purpose of clinical diagnosis [LP-MIC-ROTAT] and ImmunoCard STAT! Rotavirus kit Microbial serology for: Anti-HBs CMV IgG CMV IgM HAVAb IgG HAVAb IgM Anti-HBc HBsAg Qualitative Anti-HCV HIV Ag/Ab COMBO Rubella IgG Rubella IgM Chemiluminescence [LP-MIC-ARCHITECT] [LP-MIC-AHBS] [LP-MIC-CMVG] [LP-MIC-CMVM] [LP-MIC-HAVG] [LP-MIC-HAVM] [LP-MIC-HBcAb] [LP-MIC-HBV] [LP-MIC-HCV] [LP-MIC-HIV] [LP-MIC-RubellaIgG] [LP-MIC-RubellaIgM] and Abbott Architect i2000sr Assessment Manager: PW Page 6 of 8
Faecal extracts Serological examination activities for the purpose of clinical diagnosis Microbial serology for: Toxoplasma competition EBV EBNA IgG EBV VCA/EA IgG EBV VCA IgM Measles IgG Varicella zoster virus (VZV) IgG Syphilis antibodies Anti-streptolysin O titre (ASOT) Rapid plasma regain (RPR) Antibodies to Treponema pallidum (TPPA) Clostridium difficile glutamate dehydrogenase (GDH) C. difficile toxins A & B Enzyme Immunoassay combined with final fluorescent detection (ELFA) [LP-MIC-VIDAS] [LP-MIC-TOXOV] [LP-MIC-EBVEBNA] [LP-MIC-EBVVACG] [LP-MIC-EBVVCAM] [LP-MIC-MEASIgG] [LP-MIC-VZVG] [LP-MIC-SyphilisTP] and Biomerieux VIDAS Manual agglutination method using SOP: [LP-MIC-ASO] and BioKit Rheumajet ASO Manual flocculation method using SOP : [LP-MIC-SyphilisTP] and BioKit RPR Reditest Particle-agglutination method using SOP: [LP-MIC-SyphilisTP] and SERODIA TP.PA Automated EIA using SOP: [LP-MIC-CDIFF] and Alere DS2 Assessment Manager: PW Page 7 of 8
from males Endocervical swab Urethral swab Vaginal swab Serological examination activities for the purpose of clinical diagnosis Molecular diagnostics Detection of ribosomal RNA from: Chlamydia trachomatis Neisseria gonorrhoeae Nucleic acid amplification technique (NAAT) using SOP: [LP-MIC-PANTHER] and# Hologic Panther END Assessment Manager: PW Page 8 of 8