Maximising Matrix benefits from enzymes, options for feed producers. AB Vista enzyme application Rob ten Doeschate Technical Director EMEA INDUSTRY PRESSURES Regionally variable & cross-linked
INGREDIENT PRICES ARE SPIKING UP Source: IndexMundi, 2018 OFFERING A FLEXIBLE ENZYME STRATEGY Apply enzyme on top for improved performance
OFFERING A FLEXIBLE ENZYME STRATEGY MMN Apply a nutrient credit, maintain performance and save cost Apply enzyme on top for improved performance UNDERSTANDING THE DIET TO OPTIMISE DIGESTION
WHAT IS FIBRE? Choct, 2015 Choct, 2015 EFFECT OF FIBRE IN ANIMAL NUTRITION starch protein lipid Annison & Choct (1991). Antinutritive activities of cereal nonstarch polysaccharides in broiler diets and strategies minimizing their effects
FIBRE HELPS GUT DEVELOPMENT Insoluble NSP Soluble NSP Insoluble NSP Soluble NSP Jiménez-Moreno et al, 2009 TYPES AND LEVELS OF NSP IN CEREAL GRAINS Source: AB Vista
XYLANASES HAVE THREE MAIN MECHANISMS Reduce digesta viscosity Improve nutrient digestibility Influence intestinal fermentation Helps prime the gut for beneficial bacteria NOT ALL XYLANASES CAN PRODUCE THE RIGHT AXOS AB Vista Internal Data, 2015
ECONASE XT INFLUENCES INTESTINAL FERMENTATION IN BROILERS Gonzales Ortiz et al., 2016 XYLANASE IMPROVES AMINO ACID DIGESTIBILITY Effect of xylanase on ileal amino acid digestibility (~538 observations) 60 % improvement in IAAD with xylanase 50 40 30 20 10 Efficacy drops ~2% for every 10% increase in control digestibility 90% control digestibility xyl +3% 80% control digestibility xyl +5% 0 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1-10 Control IAAD Cowieson and Bedford, 2009 19 peer reviewed studies published between 1998 and 2009
CONSISTENT ENERGY EFFECT OF XYLANASE IN CORN- SOY DIETS USP BRZ CQS USA CQS USA SPR USA UFV BRZ MU NZ CQS USA TAM USA USP BRZ USP BRZ RES POL UFV BRZ * * PC vs. NC, P < 0.05; NC vs. NC+EXT, P < 0.05; PC vs. NC+EXT, NS *PC and NC+EXT vs. NC, NS Source: AB Vista PHYTATE REDUCES NUTRIENT DIGESTIBILITY IN FEED Phytate is the primary source of phosphorus in feedstuffs. The compound also binds to important minerals, amino acids and proteins. As monogastrics do not break down phytate efficiently, many of the vital nutrients contained within and bound to the molecule are under-utilised or excreted as waste.
PHYTATE IS PRESENT IN ALL FEED INGREDIENTS Phytic P, (%) > 100,000 samples tested Source: AB Vista PHYTATE REDUCES AMINO ACID AND PROTEIN DIGESTIBILITY IN SEVERAL WAYS Reduces activation of pepsin enzyme for protein breakdown Increases endogenous losses resulting in lower amino acid absorption Binds to proteins Reduces NA/K pump activity key for amino acid absorption
PEPSIN ACTIVITY IS LIMITED BY PHYTATE Yu et al. (2012) QUANTUM BLUE HAS A HIGH AFFINITY FOR PHYTATE: OPTIMISED FOR HIGH DOSE APPLICATION Available phosphorus release (avp%) 90% phytate breakdown Source: Suppliers published data
Developing Maximum Matrix Nutrition Digestibility studies Large effects of high Quantum Blue dose on AA digestibility Dose response studies for main amino acids Substantial potential for AA replacement Results include confidence limits Consolidated validation matrix Further safety margins applied, realistic AA and AME replacement values Test with enzyme combination Final matrix recommendations REDUCE FEED COSTS ASSOCIATED WITH AMINO ACIDS WHILE MAINTAINING FCR IN BROILERS D0-21 0 FTU 2,000 FTU 81.8% 98.3% Dig methionine and cysteine Walk and Rama Rao, 2018. Poultry Science
POTENTIAL TO SPARE M+C, LYS AND THR IN POULTRY DIETS Predicted response of Quantum Blue and digestible amino acids in poultry Response variable % of M+C spared % of Lys spared % of Thr spared BW gain, d0-42 15.9 8.0 12.5 FCR, d0-42 9.9 11.4 2.9 Carcass weight, d42 * 13.6 * Breast weight, d42 * * * BW gain, d0-21 17.2 15.9 4.0 FCR, d0-21 14.0 19.2 2.0 AID amino acid, d21 16.3 8.1 * Average 14.7% 12.7% 5.4% Summary of 6 trials in poultry 95% confidence limits applied. POTENTIAL TO SPARE LYS, THR AND TRP IN PIG DIETS Predicted response of Quantum Blue and digestible amino acids in pigs FTU/kg QB 3000 FTU 3000 FTU 2200 FTU Pigs (kg) 25-50 kg 30-60 kg 18-55kg Amino acid Lysine release Thr release Trp release ADG 0.090 0.050 0.026 G : F 0.080 0.060 0.017 Average 0.085 0.055 0.022 Summary of 3 trials in pigs
ENZYME INFLUENCE ON PROTEIN AND STARCH DIGESTIBILITY Sorghum based diet + Xylanase + Phytase + Protease + Xylanase + Phytase + Xylanase + Protease + Phytase + Protease + Xylanase + Phytase + Protease Ileal digestibility Protein (%) 72 c 75 bc 78 ab 75 bc 81 a 79 ab 78 ab 78 ab Ileal digestibility Starch (%) 75 c 83 b 86 ab 83 b 89 a 86 ab 88 a 87 a abc P<0.05 Enzymes are not additive why use a protease? Sultan, 2011 BIOLOGICAL LIMITATION: ANIMAL REQUIREMENT Lee et al., 2018. Poultry Science.
QUANTUM BLUE AND ECONASE XT WORK TOGETHER TO IMPROVE NUTRIENT DIGESTIBILITY AND PERFORMANCE N = 76 Diet P < 0.0001 Site P < 0.0001 Walk et al., 2015. 20 th European Symposium on Poultry Nutrition OFFERING A FLEXIBLE ENZYME STRATEGY MMN Apply a nutrient credit, maintain performance and save cost Apply enzyme on top for improved performance
MAXIMUM MATRIX NUTRITION: TARGETING OPTIMUM NUTRIENT UTILISATION 2000 FTU/kg Potential savings Poultry $15-27/tonne or $0.05 per bird* Swine $20-30/tonne or $0.23 per pig* 9600 BXU/kg *compared to an industry control diet with 500 FTU/kg phytase and 9600 BXU/kg xylanase POULTRY MMN DELIVERS A REDUCTION IN FEED COST WHILST MAINTAINING ANIMAL PERFORMANCE Trial Report: Quantum Blue Broilers 139
POULTRY MAXIMUM MATRIX NUTRITION IMPROVES AMINO ACID AND P EFFICIENCY Trial Report: Quantum Blue Broilers 139 POULTRY MAXIMUM MATRIX NUTRITION ALLOWS FOR A 3.5% REDUCTION IN PRODUCTION COSTS Trial Report: Quantum Blue Broilers 139
SWINE MAXIMUM MATRIX NUTRITION: PERFORMANCE P1 pig performance Trial Report: Quantum Blue Pigs 817 SWINE MAXIMUM MATRIX NUTRITION: PERFORMANCE Overall pig performance Trial Report: Quantum Blue Pigs 817
SWINE MAXIMUM MATRIX NUTRITION: EFFICIENCY NC + MMN Trial Report: Quantum Blue Pigs 817 MONITOR PHYTATE LEVELS TO ENSURE THERE IS ENOUGH AVAILABLE SUBSTRATE FOR HIGH MATRIX APPLICATION Phytate levels vary in the diet, so understanding levels in ingredients or complete feed allows for optimum phytase use in diets to deliver reliable cost savings AB Vista Feed Quality Service provides you with the phytate-p level in raw materials and finished feeds. This allows you to use the optimal amount of phytase, depending on the phytate-p level present BENEFITS CAN INCLUDE: Reduce the cost of feed formulation by optimising the P matrix from phytase Understand the variability of phytate levels in raw materials and feeds
MAXIMUM MATRIX NUTRITION RECOMMENDATION - BROILERS Quantum Blue (FTU/kg) 1500 2000 Econase XT (BXU/kg) 9600 16000 9600 16000 AvP, % 0.221 0.221 0.240 0.240 Ca, % 0.243 0.243 0.264 0.264 Na, % 0.052 0.052 0.056 0.056 ME low visc., kcal/kg Up to 117 Up to 142 Up to 122 Up to 147 ME high visc., kcal/kg Up to 145 Up to 182 Up to 150 Up to 187 Protein, % 0.692 0.778 0.734 0.820 M+C, % 0.052 0.055 0.056 0.059 Lys, % 0.028 0.031 0.029 0.033 Thr, % 0.047 0.051 0.051 0.054 Trp, % 0.025 0.026 0.027 0.028 Ile, % 0.036 0.039 0.039 0.042 MAXIMUM MATRIX NUTRITION RECOMMENDATION - PIGS Quantum Blue (FTU/kg) 1500 2000 Econase XT (BXU/kg) 12000 16000 20000 12000 16000 20000 AvP, % 0.221 0.221 0.221 0.240 0.240 0.240 Ca, % 0.243 0.243 0.243 0.264 0.264 0.264 Na, % 0.052 0.052 0.052 0.056 0.056 0.056 ME low visc., kcal/kg Up to 128 Up to 142 Up to 152 Up to 133 Up to 147 Up to 157 ME high visc., kcal/kg Up to 161 Up to 182 Up to 197 Up to 166 Up to 187 Up to 203 Protein, % 0.728 0.778 0.814 0.770 0.820 0.856 M+C, % 0.053 0.055 0.056 0.057 0.059 0.060 Lys, % 0.029 0.031 0.033 0.031 0.033 0.034 Thr, % 0.049 0.051 0.053 0.052 0.054 0.056 Trp, % 0.025 0.026 0.026 0.027 0.028 0.028 Ile, % 0.037 0.039 0.040 0.040 0.042 0.043
Maximising Matrix benefits from enzymes, options for feed producers. AB Vista enzyme application Rob ten Doeschate Technical Director EMEA