Supplemental Information. Proprioceptive Opsin Functions. in Drosophila Larval Locomotion

Similar documents
Nature Neuroscience: doi: /nn Supplementary Figure 1. Neuron class-specific arrangements of Khc::nod::lacZ label in dendrites.

Nature Medicine doi: /nm.2860

effects on organ development. a-f, Eye and wing discs with clones of ε j2b10 show no

SUPPLEMENTARY INFORMATION

marker. DAPI labels nuclei. Flies were 20 days old. Scale bar is 5 µm. Ctrl is

glial cells missing and gcm2 Cell-autonomously Regulate Both Glial and Neuronal

Department of Cell and Developmental Biology, Vanderbilt University, Nashville, TN

Supplementary Figure 1. Flies form water-reward memory only in the thirsty state

50mM D-Glucose. Percentage PI. L-Glucose

Nature Biotechnology: doi: /nbt Supplementary Figure 1. Analysis of hair bundle morphology in Ush1c c.216g>a mice at P18 by SEM.

Supplementary Table 1. List of primers used in this study

Supporting Information

Regulation of sleep plasticity by a thermo-sensitive circuit in Drosophila

Supplemental Information. Otic Mesenchyme Cells Regulate. Spiral Ganglion Axon Fasciculation. through a Pou3f4/EphA4 Signaling Pathway

Supplementary Figure 1. Procedures to independently control fly hunger and thirst states.

Supplementary Figure 1 hlrrk2 promotes CAP dependent protein translation.

Nature Neuroscience: doi: /nn Supplementary Figure 1

Supplementary Figure 1 Madm is not required in GSCs and hub cells. (a,b) Act-Gal4-UAS-GFP (a), Act-Gal4-UAS- GFP.nls (b,c) is ubiquitously expressed

100 mm Sucrose. +Berberine +Quinine

Zhu et al, page 1. Supplementary Figures

Supplementary Information

Supplementary Figure 1. SC35M polymerase activity in the presence of Bat or SC35M NP encoded from the phw2000 rescue plasmid.

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION

Supporting Online Material for

* * A3027. A4623 e A3507 A3507 A3507

Rescue of mutant rhodopsin traffic by metformin-induced AMPK activation accelerates photoreceptor degeneration Athanasiou et al

SUPPLEMENTARY INFORMATION

Supplementary Figure 1. Gene schematics of hyls-1, gasr-8 and k10g6.4, and TEM analysis of TFs in WT and hyls-1 cilia. (a) Gene structure of hyls-1,

SUPPLEMENTARY INFORMATION Glucosylceramide synthase (GlcT-1) in the fat body controls energy metabolism in Drosophila

Supplementary Figures

Edited by J. Richard McIntosh, University of Colorado, Boulder, CO, and approved June 17, 2016 (received for review November 13, 2015) Results

T H E J O U R N A L O F C E L L B I O L O G Y

Supplementary Information

Table S1. Gal4 Driver Lines in This Study, Related to Figures 1, 2, 3, 4, 5, 6, S1, S2, S3, S4, S5, and S6

Head of College Scholars List Scheme. Summer Studentship Report Form

Diabetic pdx1-mutant zebrafish show conserved responses to nutrient overload and anti-glycemic treatment

Supplementary Figure 1: Signaling centers contain few proliferating cells, express p21, and

Supplements. Figure S1. B Phalloidin Alexa488

ROCK/Cdc42-mediated microglial motility and gliapse formation lead to phagocytosis of degenerating dopaminergic neurons in vivo

Concerted action of CB1 cannabinoid receptor and Deleted in Colorectal Cancer (DCC) in axon guidance

Response to Mechanical Stress Is Mediated by the TRPA Channel Painless in the Drosophila Heart

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY FIGURE LEGENDS

Figure S1. (A) SDS-PAGE separation of GST-fusion proteins purified from E.coli BL21 strain is shown. An equal amount of GST-tag control, LRRK2 LRR

Capu and Spire Assemble a Cytoplasmic Actin Mesh

Supplementary Information

Supplemental Information. Ciliary Beating Compartmentalizes. Cerebrospinal Fluid Flow in the Brain. and Regulates Ventricular Development

T H E J O U R N A L O F C E L L B I O L O G Y

The Drosophila melanogaster life cycle

Nature Neuroscience: doi: /nn Supplementary Figure 1

Name: Answer Key. Question 1.

A Drosophila model for alcohol reward. Supplemental Material

Lecture: Introduction to nervous system development

Supplementary Fig. 1 V-ATPase depletion induces unique and robust phenotype in Drosophila fat body cells.

Supplementary information

1) Drop off in the Bi 150 box outside Baxter 331 or to the head TA (jcolas).

Glial-Derived Prodegenerative Signaling in the Drosophila Neuromuscular System

SUPPLEMENTARY INFORMATION

Why am I working in a Drosophila lab? Dr. Stefan Pulver, Brandeis University

Supplemental Data. Wu et al. (2010). Plant Cell /tpc

Wenqin Hu, Cuiping Tian, Tun Li, Mingpo Yang, Han Hou & Yousheng Shu

Supplementary Figure 1. EC-specific Deletion of Snail1 Does Not Affect EC Apoptosis. (a,b) Cryo-sections of WT (a) and Snail1 LOF (b) embryos at

XUE HUI Department of Histology& Embryology, Basic Medicine College of Jilin University

Supplementary Materials

Supplementary Figure 1

Lack of cadherins Celsr2 and Celsr3 impairs ependymal ciliogenesis, leading to fatal

Supplementary Materials for

Supplementary Information

Glia and Muscle Sculpt Neuromuscular Arbors by Engulfing Destabilized Synaptic Boutons and Shed Presynaptic Debris

Supplementary Figure S1: TIPF reporter validation in the wing disc.

Supplemental Information. Memory-Relevant Mushroom Body. Output Synapses Are Cholinergic

CONTRACTING ORGANIZATION: Cold Spring Harbor Laboratory Cold Spring Harbor, NY 11724

Test of visual pathway function

Role of Autophagy in Glycogen Breakdown and Its Relevance to Chloroquine Myopathy

The microrna bantam Functions in Epithelial Cells to Regulate Scaling Growth of Dendrite Arbors in Drosophila Sensory Neurons

Supplementary Figure 1. Nature Neuroscience: doi: /nn.4547

Figure S1: CtxA interacts with Notch and the endogenous Drosophila camp machinery. Figure S1, related to Figure 1.

Supplemental Figure 1. Quantification of proliferation in thyroid of WT, Ctns -/- and grafted

CHAPTER 10 THE SOMATOSENSORY SYSTEM

Cellular and genetic bases of cold nociception and nociceptive sensitization in Drosophila larvae

SUPPLEMENTARY INFORMATION

GFP/Iba1/GFAP. Brain. Liver. Kidney. Lung. Hoechst/Iba1/TLR9!

The Serotonergic Central Nervous System of the Drosophila Larva: Anatomy and Behavioral Function

doi: /nature09554

SUPPLEMENTARY INFORMATION

THE VISUAL WORLD! Visual (Electromagnetic) Stimulus

SUPPLEMENTARY INFORMATION

Meigo governs dendrite targeting specificity by modulating Ephrin level and N-glycosylation

Nature Neuroscience: doi: /nn Supplementary Figure 1. Large-scale calcium imaging in vivo.

Supplemental Materials Molecular Biology of the Cell

High sensitivity rod photoreceptor input to blue-yellow color opponent pathway in macaque retina

ns ns hp761(lf); daf-28(lf) daf-28(gf) Figure S1

SUPPLEMENTARY MATERIAL

The target of rapamycin complex 2 controls dendritic tiling of Drosophila sensory neurons through the Tricornered kinase signalling pathway

Supplementary Figure 1

Miniature Neurotransmission Regulates Drosophila Synaptic Structural Maturation

Supplemental Data. Wang et al. (2013). Plant Cell /tpc

AMP-activated protein kinase (AMPK) is a metabolic enzyme that is essential for

Supplementary Fig. 1. The Brown Norway rat has higher coronary flow compared to other rat strains. Publically available data for coronary flow

Transcription:

Neuron, Volume 98 Supplemental Information Proprioceptive Opsin Functions in Drosophila Larval Locomotion Damiano Zanini, Diego Giraldo, Ben Warren, Radoslaw Katana, Marta Andrés, Suneel Reddy, Stephanie Pauls, Nicola Schwedhelm-Domeyer, Bart R.H. Geurten, and Martin C. Göpfert

Supplemental Information Proprioceptive Opsin Functions in Drosophila Larval Locomotion Damiano Zanini, Diego Giraldo, Ben Warren, Radoslaw Katana, Marta Andrés, Suneel Reddy, Stephanie Pauls, Nicola Schwedhelm-Domeyer, Bart R. H. Geurten, and Martin C. Göpfert Figure S1 (Related to Figure 1) Figure S1. Locomotion and contraction defects in opsin mutants. (A) Locomotion. (B) Body contraction. Same data as in Figure 1B,C. Instead of comparing to wild-type, however, comparisons are shown here against w 1118 larvae, 2

which crawl slightly slower than the wild-type. Opsin effects can nonetheless be discerned. N = 30 w 1118 larvae, otherwise as specified in the legend to Fig. 1B,C. Note that genetic rescues partly show significant differences from controls because they are partly outperforming them. Colour codes, abbreviations, and statistics as in Fig. 1B,C. 3

Figure S2 (Related to Figure 2) Figure S2. Opsin expression and localization in proprioceptors. (A, B) Additional examples to those shown in Fig. 2. (A) ninae (left) and Rh6 (right) expression in lch5. (B) Anti-NINAE (left) and Anti-Rh6 (right) staining of the inner dendritic segments of lch5 receptors, whereby the receptor neurons are counterstained with anti-hrp. For NINAE, staining is shown for wild-type (WT) larvae, whereas a staining from a Rh6 1, P{Rh6 + } rescue larva is shown for Rh6. Lch5 receptors 2 and 4 show only weak anti- Rh6 staining that can be seen at higher magnification as shown in Fig. 2C. For additional details, see legend to Fig. 2A,B. (C) Rh7 opsin gene expression in chordotonal proprioceptors. Left: Sketch of lch5, depicting chordotonal receptor dendrites (D), somata (S), and axons (A). Right: GFP signals in lch5 receptors revealed driving a UAS-6xGFP reporter with PBac{IT.Gal4}Rh7 0074-G4, a Gal4 insertion in the coding region of the Rh7 gene. Receptors of lch5 are counterstained with anti-hrp and GFP signals are enhanced with anti-gfp. 4

Figure S3 (Related to Figure 4) Figure S3. Ciliary ion channel localization in wild-type proprioceptors. Lch5 stained with anti-iav, anti-nompc, and anti-hrp antibodies. Iav localizes to the proximal ciliary region, between the two HRP bands (arrowheads, left panel). NOMPC localizes to the cilium tips, distally to the second HRP band, yet it protrudes in the proximal ciliary region in lch5 receptors 2 and 4 (arrows, middle panel), which also show weak anti-rh6 signals (Fig. 2C). The extended ciliary NOMPC localization in lch5 receptors 2 and 4 was consistently seen in controls and wild-type, documenting a partial overlap between Iav and NOMPC in some receptor cilia of lch5. 5

Figure S4 (Related to Figures 1 and 3) Figure S4. Locomotion and proprioceptor latency. (A) Locomotion effects caused by ablating chordotonal (Ch) receptors through crossing UAS-hid, rpr to the chordotonal receptor driver Dhc93AB-Gal4. (B) Locomotion defects caused by RNAi 6

knockdown of ninae in chordotonal receptors by crossing UAS-ninaE-RNAi to the chordotonal receptor driver dnai2-gal4. (C) Locomotion phenotypes of ninae 17, Rh6 1 double mutants compared to the single mutants. (D) Locomotion in santa-maria 1 mutants that fail to synthesize the retinal chromophore compared to wild-type (WT) and w 1118 controls. (E) Locomotion effects caused by ablating TRPA1-expressing neurons by crossing UAS-hid, rpr to trpa1-gal4. (F) Locomotion in ninae 17 and Rh6 1 mutants compared to wild-type and w 1118 controls at an elevated temperature of 29 C where opsins do not contribute to thermosensing. (G) Targeted ablation of the chordotonal receptors by driving UAS-hid, rpr with Dhc93AB-Gal4. Actin-based rods supporting lch5 receptor dendrites are stained with phalloidin (magenta) and neurons with anti-hrp (yellow). Whereas the actin-based rods persist, lch5 receptors are lost. (H) lch5 response latency. Example traces showing lch5 compound action currents (black) in response to a 3 m deflection (red) applied to the stimulus-conveying cap cells. Sample size in panels (A-H): N = 20 to 43 larvae per strain. Significant differences (Fisher's exact permutation tests with Bonferroni correction): * = p < 0.05, ** = p < 0.01, *** = p < 0.001, ns = not significant. 7

2024 © healthdocbox.com Privacy Policy | Terms of Service | Feedback