www.sciencesignaling.org/cgi/content/full/8/407/ra127/dc1 Supplementary Materials for Loss of FTO in adipose tissue decreases Angptl4 translation and alters triglyceride metabolism Chao-Yung Wang,* Shian-Sen Shie, Ming-Shien Wen, Kuo-Chun Hung, I-Chang Hsieh, Ta-Sen Yeh, Delon Wu The PDF file includes: *Corresponding author. E-mail: cwang@ocean.ag Published 15 December 2015, Sci. Signal. 8, ra127 (2015) DOI: 10.1126/scisignal.aab3357 Fig. S1. FTO protein distribution in human and mice. Fig. S2. FTO abundance after 2 and 14 days on a high-fat diet. Fig. S3. Generation of adipocyte-specific FTO knockout mice. Fig. S4. Analysis of tibial length, adipocyte size, energy expenditure, thyroid function, body weight, and paired feeding experiments. Fig. S5. Relative mrna expression of brown fat genes in AFO and wild-type mice. Fig. S6. Glucose tolerance and insulin sensitivity. Fig. S7. Lipidomics analysis of white fat tissue in AFO and wild-type mice. Fig. S8. FTO protein abundance during adipocyte differentiation. Fig. S9. LPL-mediated lipoprotein-dependent fatty acid uptake. Fig. S10. RNA immunoprecipitation experiments. Fig. S11. RNA expression in white adipose tissue. Fig. S12. RNA EMSAs for Angptl4 mrna and FTO protein. Fig. S13. DAA treatment and Angptl4 abundance in AFO adipocytes. Fig. S14. m 6 A pattern in Angptl4 mrna. Fig. S15. Polysome profiles and Angptl4 RNA abundance in control and FTO knockdown MCF-7 cell lines. Fig. S16. Serum triglyceride concentrations in wild-type and AFO mice after inguinal fat pad injections of control adenovirus or adenovirus expressing Angptl4.
Fig. S1. FTO protein distribution in human and mice. Left panel: Western blot analysis of FTO protein abundance in human tissue samples. Right panel: Western blot analysis of FTO protein expression in mouse adipose tissue. SF, subcutaneous fat; VF, visceral fat; BF, brown fat. Data are representative of two independent experiments.
Fig. S2. FTO abundance after 2 and 14 days on a high-fat diet. (A) mrna analysis of PPAR- and FTO from subcutaneous white fat (WF), visceral WF, and brown fat (BF) from WT mice fed chow diet (CD), 2-d HFD, or 14-d HFD (n = 4 mice per diet). * P < 0.05, 2d- HFD or 14-d HFD, compared to CD. (B) Protein analysis by Western blot of PPAR- and
FTO from subcutaneous fat, visceral fat, and brown fat from WT mice fed chow diet, 2-d HFD, or 14-d HFD (n = 4 mice per diet). * P < 0.05, 2d-HFD or 14-d HFD, compared to CD. (C, D) FTO mrna and protein abundance in adipose tissue from WT mice that received the PPAR- inhibitor GW9662 for 1 d after being fed with CD or 2-d HFD (n = 4 mice per diet and treatment). * P < 0.05, HFD-GW9662 compared to HFD-C. Data represent mean ± s.e.m. Statistical analysis was done using two-sample Mann-Whitney test.
Fig. S3. Generation of adipocyte-specific FTO knockout mice (A) Wild-type, floxed, and deleted FTO gene loci with targeting vector. (B) Absolute FTO mrna copy number (copies/10 ng total RNA) in AFO and WT (FTO f/f) mice (n = 3 mice per genotype). Data represent mean ± s.e.m. * P < 0.05, Adiponectin-Cre FTO f/f compared to FTO f/f.
Statistical analysis was done using two-sample Mann-Whitney test. (C) Protein abundance of FTO and actin in AFO and WT mice (n = 3 mice per genotype). Fig. S4. Analysis of tibial length, adipocyte size, energy expenditure, thyroid function, body weight, and paired feeding experiments. (A) Tibial length (TL) and adipocyte size quantification for epididymal white adipocytes from 20-week-old mice (n=10 mice per
genotype). Energy expenditure (EE) in 8-week-old mice (n=12 mice per genotype), adjusted for variation in lean mass using multiple linear regression (ANCOVA). Data represent means ± s.e.m. * P < 0.05, adiponectin-cre mice compared to WT mice. The FTO f/f and AFO mice data presented here were from Fig. 1D, 1G, and 2G to enable comparison with the other genotypes. (B) Thyroid function of AFO and WT (FTO f/f) mice (n = 6 mice per group). Data represent mean ± s.e.m. NS, not significant, AFO compared to FTO f/f (twosample Mann-Whitney test). (C) Body weights of AFO and FTO f/f littermate mice on chow diet, HFD, and paired feeding experiments (n = 10 12 mice per group). Pair-fed mice received the same amount of calories in a high-fat diet as mice fed with the chow diet. Data represent mean ± s.e.m. AFO on the high-fat diet weighed significantly more than FTO f/f on the high-fat diet by two-way ANOVA with repeated measures (P = 0.03); *, P < 0.05, AFO paired feeding compared to FTO f/f paired feeding (Student s unpaired t-test). Fig. S5. Relative mrna expression of brown fat genes in AFO and wild-type mice. Data represent mean ± s.e.m. *, P < 0.05 AFO compared to FTO f/f brown fat. NS, not significant. n = 6 mice per group. Statistical analysis was done using two-sample Mann-Whitney test.
Fig. S6. Glucose tolerance and insulin sensitivity. (A) Glucose tolerance and (B) insulin sensitivity in AFO and WT (FTO f/f) mice fed with chow diet (n = 8 mice per group). Data represent mean ± s.e.m.
Fig. S7. Lipidomics analysis of white fat tissue in AFO and wild-type mice. Epididymal white fat was isolated and lipids were extracted and analyzed with lipidomics (n = 3 mice per group). Data represent mean ± s.e.m. *, P < 0.05 AFO compared to FTO f/f. Statistical analysis was done using two-sample Mann-Whitney test.
Fig. S8. FTO protein abundance during adipocyte differentiation. Western blot analysis of FTO and actin abundance during the in vitro differentiation of preadipocytes from WT and FTO-knockout mice to mature adipocytes (n = 3 mice per genotype). WT, wild-type adipocytes; KO, FTO-knockout adipocytes.
Fig. S9. LPL-mediated lipoprotein-dependent fatty acid uptake. (A) Adipocytes from AFO or WT (FTO f/f) mice were treated with or without orlistat and incubated with vehicle, intralipid, or oleic acid-rich bovine serum albumin (OA-BSA). Intracellular triglyceride was analyzed and normalized to cell numbers (n = 3 mice per genotype and treatment). Data represent mean ± s.e.m. (B D) Intracellular lipids were analyzed by lipidomics (n = 4 mice
per group). Data represent mean ± s.e.m. *, P < 0.05 AFO compared to FTO f/f treated with vehicle. Statistical analysis was done using two-sample Mann-Whitney test. A Isolation of visceral white fat IP RNA purification RNA sequence Flag reads vs. IgG reads Enriched RNA list F-FTO Bowtie mapping Selection of RNA target related to lipid homeostasis RNA-IP Immunoprecipitation with Flag or IgG beads Read counts for known & new RNAs Normalization Flag vs. IgG Cross reference to Microarray lists from WT/AFO mice B C log (IgG-IP) 2 15 10 5 0-5 -10-15 -20-25 -30-35 -35-30 -25-20 -15-10 -5 0 5 10 15 log 2(Flag-IP) Adcre - Adcre + D STARD3 SNX17 SLS27A4 SCARF1 PRKAB1 PPARD PMVK PCSK9 OXCT2A MVK LCAT INSIG1 IDI2 FDPS ECHS1 CYP46A1 CYP11A1 CYB5R3 APOA4 APOA2 APOA1 ANGPTL4 ACSM5 ACOX3 ACOX1 ACOT8 ACOT2 ACADS ACADL ACAA1A ABCG1 Flag-FTO IP Lipid homeostasis related RNA enrichment ratios 0 1 2 3 4 5 6 Fig. S10. RNA immunoprecipitation experiments. (A) Schematic diagram of the RNAimmunoprecipitation (IP) procedure. (B) Scatterplot of Flag-FTO-IP and RNA-sequence data, in which normalized and log-transformed read numbers for each known transcript are
plotted against control IgG-IP data. The FLAG-FTO-IP analysis was performed twice. (C) Microarray of white adipose tissues from AFO and WT (FTO f/f) mice (n = 3 mice per genotype). (D) The enrichment ratios of FTO-associated RNAs with functions related to lipid homeostasis. Fig. S11. RNA expression in white adipose tissue. Quantitative PCR of gene expression in white adipose tissue from AFO and WT (FTO f/f) mice (n = 3 mice per genotype). Data represent mean ± s.e.m. NS, not significant. Statistical analysis was done using two-sample Mann-Whitney test.
Fig. S12. RNA EMSAs for Angptl4 mrna and FTO protein. (A) Two segments of the Angptl4 3ʹ UTR were in vitro transcribed and incubated with different amounts of FTO protein. RNA-protein complexes were analyzed together with free probe, unlabeled RNA, and FTO protein control. (B) RNA EMSA analysis of Angptl4 mrna in free probes (F), FTO protein-angptl4 mrna probe complexes (FTO), and nonlabeled Angptl4 mrna
competitor + FTO protein-angptl4 mrna probe complexes (C). (C) Angptl4 3ʹ UTR segments were incubated with adipose tissue protein lysates from wild-type (WT) or FTOknockout (KO) mice and analyzed with EMSA. Data are representative of three independent experiments. Fig. S13. DAA treatment and Angptl4 abundance in AFO adipocytes. (A) Treatment of primary adipocytes from WT (FTO f/f) and AFO mice with vehicle or DAA. Representative Western blot for Angptl4 and m 6 A Northern-Western blot for m 6 A abundance in total RNA (n = 4 mice per genotype). (B) Angptl4 m 6 A abundance analyzed by m 6 A immunoprecipitation and real-time quantitative PCR (n = 3 mice per genotype and treatment). (C) Angptl4 mrna abundance analyzed by real-time quantitative PCR (n = 3
mice per genotype and treatment). (D) Quantification of Angptl4 protein abundance by Western blot (n = 3 mice per genotype and treatment). Data represent mean ± s.e.m. *, P < 0.05, AFO compared to WT. NS, not significant, AFO+DAA compared to WT+DAA. Statistical analysis was done using two-sample Mann-Whitney test. Fig. S14. m 6 A pattern in Angptl4 mrna. mrna purified from total RNA was subjected to immunoprecipitation by m 6 A antibody. Immunoprecipitated mrnas were analyzed by RNA sequencing and visualized with an integrative genomics viewer (n = 2 independent experiments).
Fig. S15. Polysome profiles and Angpt4 RNA abundance in control and FTO knockdown MCF-7 cell lines. (A) Polysome profiles from control MCF-7 (MCF-7 sh) and FTO knock-down MCF-7 (MCF-7 AS2) cell lines (n = 3 independent experiments). (B) Relative Angptl4 RNA abundance in RNA extracts from polysome fractions of MCF-7 sh and MCF-7 AS2 cell lines (n = 3 independent experiments). Data represent mean ± s.e.m. Yellow-shaded areas indicate polysome fractions.
Fig. S16. Serum triglyceride concentrations in wild-type and AFO mice after inguinal fat pad injections of control adenovirus or adenovirus expressing Angptl4. (A) Western blots and (B) relative Angptl4 mrna abundance of inguinal adipose tissues from WT (FTO f/f) and AFO mice 2 weeks after injections of control adenovirus (Ad516) or adenovirus expressing Angptl4 (AdAngptl4) (n = 6 mice per genotype and adenovirus injection). Data represent mean ± s.e.m. (C) Serum triglyceride concentrations of WT and AFO mice fed with chow diet or high-fat diet (HFD) 2 weeks after inguinal fat pad injections of control adenovirus (Ad516) or adenovirus expressing Angptl4 (AdAngptl4) (n = 6 mice per genotype and adenovirus injection). Data represent mean ± s.e.m. NS, not significant. * P < 0.05, AdAngptl4 with HFD compared with Ad516 with HFD. Statistical analysis was done using two-sample Mann-Whitney test.