2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Microbiology Department West Hertfordshire Hospitals NHS Trust Watford General Hospital Vicarage Road Watford Hertfordshire WD18 0HB Contact: Mrs Arezou Fanaie Tel: +44 (0) 1923 217586 Fax: +44 (0) 1923 217598 E-Mail: Arezou.fanaie@whht.nhs.uk Testing performed at the above address only Website: www.westhertshospitals.nhs.uk TISSUES Wound & skin swabs Upper Respiratory Swabs, Cannula,Tips, Pus, Exudates Ear Swab Pernasal swab Genital tract swab Respiratory Fluids (Sputum,Antral, Nasopharyngeal Aspirates, Bronchial Washings), Cough swab Tissue & Biopsies Sterile Body Fluids Cerebrospinal Fluid (CSF) DETAIL OF ACCREDITATION diagnosis Isolation and characterisation of micro-organisms of clinical significance based on related UK Standards for Microbiology Investigations (SMIs) and manufacturer s operating instructions, where relevant, to support clinical diagnosis Manual inoculation and media culture MI/SOP106 MI/SOP58 MI/SOP111 MI/SOP52 MI/SOP60 MI/SOP57 MI/SOP47 MI/SOP36 MI/SOP49 MI/SOP87 Assessment Manager: PW Page 1 of 7
Swabs from nose, groin, perineum, wounds, hairline, tips (not urinary catheter tips), sputum and urine Respiratory fluids (Sputum,Antral, Nasopharyngeal Aspirates, bronchial washings), Tissue Blood Swabs, Tissue, fluids Respiratory fluids (Sputum,Antral, Nasopharyngeal Aspirates, Bronchial washings) Tissue, presumptive AFB isolated in-house Screening and detection of: Carbapenemase Resistant Enterobacteriaceae (CRE) Extended-Spectrum Betalactamase (ESBL) Gentamicin Resistant Organism (GRO) Methicillin Resistant Staph aureus (MRSA) Vancomycin Resistant (VRE) Pseudomonas Isolation and presumptive identification of Mycobacterium species Culture and detection of microorganisms of clinical significance Differentiation of microorganisms of clinical significance different organism types:gram positive/negative/variable, branching organisms, yeasts Presence or absence of Acid Fast Bacilli Manual inoculation and media culture MI/SOP38 Automated culture Biomerieux MB/Bact Alert system Liquid culture/fluorescent growth detection Media culture MI/SOP42 Automated culture Becton Dickinson (BD) BACTEC FX400 Liquid culture/fluorescent growth detection Media culture Liquid cultures (adult/paediatric ) MI/SOP113 Light microscope Gram staining Microscopy MI/SOP03 UV microscope Auramine fluoresent staining Fluorescent microscopy MI/SOP42 Assessment Manager: PW Page 2 of 7
CSF, Ascitic Fluid Clinical isolates cultured in-house (inc. Bacterial/yeasts/fungi) Detection and quantification of white blood cells and red cells Detection and quantification of white and red blood cells, bacteria and yeast cells Identification of microorganisms of clinical significance Light microscope Wet film microscopy Kova Counting Grid MI/SOP36 sedimax Analyser Automated urine microscopy and particle recognition MI/SOP87 Bruker Microflex Biotyper (MALDI- TOF) Mass spectrometry MI/SOP103 Bacterial isolates cultured in -house Antimicrobial susceptibility testing Media culture BD Phoenix, BD Auto Prep and EpiCenter MI/SOP107 MI/SOP110 Manual antimicrobial susceptibility testing using European Committee on Antimicrobial Susceptibility Testing (EUCAST) methodology Antibiotic disc diffusion and gradient MIC determination MI/SOP/108 MI/SOP/15 Detection of Pneumococcal soluble antigen in Detection of Legionella antigen in urine as an indicator of respiratory infection Binax Now Streptococcus kit MI/SOP62 Binax Now Legionella kit MI/SOP61 Assessment Manager: PW Page 3 of 7
Detection of Clostridium difficile GDH antigen and toxins A & B Detection of Helicobacter stool antigen. Techlab TOX A/B QUIK CHEK COMPLETE kit Lateral Flow EIA Device MI/SOP40 Proflow H.pylori test kit Manual Immunochromatographic MI/SOP/SE52 Detection of Rotavirus antigen ImmunoCard STAT test kit MI/SOP48 Mycology Skin scraping, Nail clippings, Hair samples Skin scraping, Nail clippings, Hair samples, Fluids, Tissue Microscopic determination of presence or absence of fungal elements Isolation and characterisation of micro-organisms of clinical significance Parasitology Identification/confirmation of Ova, Cysts and parasites Calcofluor-white fluorescence microscope MI/SOP/45 Manual inoculation and media culture Macroscopic and microscopic examination MI/SOP/45 Light microscope Concentrated wet film microscopy Detection of Cryptosporidium sp UV microscope Auramine fluorescent staining Detection of Enterobius vermicularis ova Detection of Schistosoma haematobium ova Sellotape slide Light Microscopy Light Microscopy Assessment Manager: PW Page 4 of 7
Serology Serum Detection of: VIDAS analyser Automated Fluorescent EIA MI/SOP/SE36 Hepatitis B surface antigen MI/SOP/SE3 Hepatitis B Surface antibody MI/SOP/SE5 Hepatitis B core antibodies MI/SOP/SE4 MI/SOP/SE7 Hepatitis C antibodies MI/SOP/SE49 HIV Duo Quick MI/SOP/SE10 Rubella IgG & IgM MI/SOP/SE11 MI/SOP/SE12 CMV IgG & IgM MI/SOP/SE8 MI/SOP/SE9 Epstein Barr Virus IgM MI/SOP/SE65 Measles IgG MI/SOP/SE37 Mumps IgG MI/SOP/SE38 Toxoplasma IgG Varicella Zoster IgG Hepatitis A Total antibody and IgM Borrelia burgdorferi IgG and IgM antibodies MI/SOP/SE13 MI/SOP/SE14 MI/SOP/SE1 MI/SOP/SE2 MI/SOP/SE56 Assessment Manager: PW Page 5 of 7
Serum Detection of: Diasorin Liaison XL random access analyser MI/SOP/SE67 Hepatitis B surface antigen MI/SOP/SE71 Hepatitis B Surface antibody MI/SOP/SE70 Hepatitis B core antibodies MI/SOP/SE89 Hepatitis C antibodies MI/SOP/SE73 HIV 1&2 antigen & antibodies* MI/SOP/SE72 Rubella IgG MI/SOP/SE69 Epstein Barr Virus IgM and IgG MI/SOP/SE83 CMV IgG & IgM MI/SOP/SE95 Varicella Zoster IgG MI/SOP/SE77 Hepatitis A Total antibody and IgM MI/SOP/SE96 Borrelia burgdorferi IgG and IgM MI/SOP/SE92 antibodies Parvovirus IgG and IgM MI/SOP/SE90 Treponema pallidum antibodies MI/SOP/SE68 Serum Serum Detection of Treponema pallidum antibodies (confirmation) Treponema pallidum antibodies (confirmation) Espline TP test kit Immuno-chromatography qualitative MI/SOP/SE51 Biokit RPR Reditest test kit Qualitative and semi-quantitative RPR MI/SOP/SE18 Serum Anti-streptococcal serology Biokit Antistreptolysin O test kit Biomerieux Strep Anti-DNase B MI/SOP/SE15 MI/SOP/SE16 Serum Mycoplasma pneumonia antibodies Serodia-MYCO II test kit Qualitative and Semi-quantitative Assay MI/SOP/SE22 Assessment Manager: PW Page 6 of 7
Naso-pharyngeal Aspirate (NPA) Detection of RSV antigen Lateral Flow EIA Device test kit Binax Now RSV antigen MI/SOP54 Whole blood Detection of latent TB QIAGEN QuantiFERON-TB Gold ELISA Thermo Plate Washer Thermo Plate Reader MI/SOP/SE46 MI/SOP/SE47 MI/SOP/SE61 END Assessment Manager: PW Page 7 of 7