Extract Vademecum Department of Medical Microbiology Status: Code: Version: Approval date: Geldig MMB-PR-04/1 1 Juni 2017 Name request Specimen Material needed Transport Bacteriology Bacterial culture Blood culture Bordetella (para)pertussis Brucella spp. (Bang disease) Aeromonas spp. E.coli O157:H7 Salmonella spp./shigella spp. Yersinia enterocolitica Campylobacter spp. Vibrio cholera Clostridium difficile CAPD (Continue Ambulant Peritoneal Dialysis) pus, punctate, tissue, superficial swabs Adults: 20 ml blood Infants: as many blood as possible nose/throat cavity swab blood, bonemarrow, tissue (fluid) 50 mldialysate Storage conditions Adults: 10 ml in aerobe and 10 ml in anaerobe bloodculture bottle Within 24h Infants: as many as possible in pediatric bloodculture bottle sterile container Blood: same as bloodculture Bloodculture bottles/ Sterile container A.s.a.p. A.s.a.p. or incubator at 36 C Other: Blood: or incubator at 36 C Turn around time 2-5 days 5 days 7 days 3 weeks Feces 2-5 days 5 days Remarks Anaerobic bacteria may take up to 10 days. Endocarditis (HACEK): 10 days Fungi: 10 days
Genital culture Neisseria gonorrhoeae Haemophilus ducreyi Helicobacter pylori culture Legionela spp. culture Inventarisation culture neonates/prom MRSA (Methycilline Resistant S. aureus), HRMO (Highly Resistant Micro organism), CPE (Carbapenemase Positive Enterobacteriaceae) Gram stain Mycology Mycology Yeasts India ink stain Feces/Parasitology urogenital samples urogenital samples ulcus swab gastric/duodenal biopsy pulmonary tissue, transtracheal punctate, bronchoscopy specimens, pleura punctate throat, ear, rectal and skin swabs throat, nose, perineum, wound, urine, respiratoire specimens diverse specimen Respiratoire samples, liquor, body fluids, urogental swabs, superficial swabs, nasopharynx, feces, skin, nail, hair (Urogenital) pus, urine, blood, feces, respiratoire samples, superficial swabs CSF, liquor sterile swab in charcoal or amies sterile swab in amies sterile container in NaCl solution Within 24h 3-5 days A.s.a.p, same day A.s.a.p, 3-5 days Do not refrigerate. 5-10 days 10 days sterile container 10 days sterile swab in amies container Within 24h A.s.a.p. or 2-5 days 3-5 days 5 weeks 2-7 days Within 24h Cryptococci
Cryptosporidium spp. Cyclospora spp. Malaria spp. blood EDTA tube Schistosoma spp. feces/urine sterile container Worms, wormeggs, cysts and protozoa Enterobius vermicularis Strongyloides Trichomonas feces tapetest feces (sputum or urine in case of hyperinfection) vagina/cervix sterile container clear tape imprint of the perianal folds attached to a clean microscope slide feces in sterile container sterile swab in amies Trypanosoma blood EDTA tube Adeno-virus/Rota-virus antigen rapid test Therapeutic Drug feces feces in sterile container Gentamicin concentration 2 ml serum coagulation tube Vancomicin concentration 2 ml serum coagulation tube Serology Within 72h same day do not cool confirmationtest: 1-2 weeks Adeno IgG 2 ml serum coagulation tube Amoeba serology. coagulation tube Aspergillus serology 2 ml serum coagulation tube Bartonella henselae 2 ml serum coagulation tube Bof (Mumps) 2 ml serum coagulation tube Bordetella (para)pertussis feces (SAF) container Within 24h coagulation tube
Borrelia burgdorferi (Lyme disease) 2 mlserum/liquor Brucella spp. (Bang disease) 2 ml serum coagulation tube Campylobacter jejuni Chikungunya virus Chlamydia spp. Coxiella burnetti Coxakie A/B-virussen coagulation tube/edta tube coagulation tube coagulation tube coagulation tube serum pair: 1st:10 days after, coagulation tube 1st symptoms & 2nd:after 2 weeks CMV (Cytomegalovirus) 2 ml serum coagulation tube 2 days Dengue virus 3 ml serum coagulation tube Difteria-, tetanus- & poliovirus Enterovirus2 ml serum EBV (Epstein Barr Virus) Pfeiffer disease Haemophilus influenzae (vaccination status) unvaccinated: serumpair: 1st: pre vaccination & 2nd: 3 weeks after vaccination vaccinated:2 ml serum, 5 ml 2 ml serum/plasma coagulation tube coagulation tube/edta tube Elisa: antigen rapid test: coagulation tube 2 ml serum coagulation tube Hanta virus 2 ml serum coagulation tube Helicobacter pylori 2 ml serum coagulation tube If antigen titer is under the protection level, repeat test with the remark "2nd sample" Only in consultation with the clinical microbiologist Hepatitis A Hepatitis C 2 ml serum coagulation tube 2 days
Hepatitis B 2 ml serum coagulation tube 3 days* If HBV screening and confirmation is positive, follow up HBV markers are tested. Hepatitis E 2 ml serum coagulation tube Within 24h HSV (Herpes Simplex Virus) 2 ml serum coagulation tube HIV (Human 2 ml serum coagulation tube 2 days Immunodeficiency Virus) HIV (Human Immunodeficiency Virus) 3 ml serum coagulation tube CITO: 1 workday Confirmation HTLV (Human T-Lymphocyte 2 ml serum coagulation tube 1-2 weeks Virus) Legionella 2 ml serum coagulation tube Legionella pneumophila antigen rapid test urine sterile container Leishmania spp. 2 ml serum coagulation tube Leptospirosis (Weill disease) 2 ml serum coagulation tube Measles virus 2 ml serum, liquor Mycoplasma pneumoniae 2 ml serum coagulation tube Parvovirus B19 3 ml serum coagulation tube Pneumococci antibodies (vaccination status) 4 ml serum coagulation tube Pneumococci antigen urine sterile container Rabies (Hondsdolheid) 5 ml serum coagulation tube RSV (Respiratoir Synctieel Virus) 2 ml serum, liquor, saliva virus transport,
Rubella virus (Rode hond) 2 ml serum coagulation tube 2 days Toxoplasma 3 ml serum coagulation tube 2 days Treponema pallidum Screening/VDRL/FTA/Lues blot VZV (Varicella Zoster Virus) Zika virus *Turn around time may be prolonged 2 ml serum coagulation tube 2 ml serum, liquor 2 ml serum 3 ml serum CITO and neonatal options available