CO 2 tolerance of Atlantic salmon post-smolts in recirculating aquaculture systems

Similar documents
Immune suppression in Atlantic salmon: smoltification, sea water transfer and breeding

HOST RESPONSE AGAINST SALMON LOUSE what have we learned so far?

Effects of water salinity and exercise on Atlantic salmon performance as postsmolts in land-based closedcontainment

Table S1 Differentially expressed genes showing > 2 fold changes and p <0.01 for 0.01 mm NS-398, 0.1mM ibuprofen and COX-2 RNAi.

Supplementary Table SI: Y strain T. cruzi infection results in the upregulation of 381 genes at the site of infection.

SUPPLEMENTAL TABLE I. Identified Proteins in Bovine Testicular Hyaluronidase Type I-S via LC-MS/MS

TNFSF13B tumor necrosis factor (ligand) superfamily, member 13b NF-kB pathway cluster, Enrichment Score: 3.57

Supplementary Table S1. Primers used for quantitative real-time polymerase chain reaction. Marker Sequence (5 3 ) Accession No.

* Kyoto Encyclopedia of Genes and Genomes.

Cetoleic acid makes pelagic fish more healthy

Supplementary Table 1. Genes analysed for expression by angiogenesis gene-array.

REACTOME: Nonsense Mediated Decay (NMD) REACTOME:72764 Eukaryotic Translation Termination. REACTOME:72737 Cap dependent Translation Initiation

T cell-mediated immunity

Hormones and Signal Transduction. Dr. Kevin Ahern

The recruitment of leukocytes and plasma proteins from the blood to sites of infection and tissue injury is called inflammation

Supplementary Figure 1.

Abstract. Introduction

KEY CONCEPT The overall process of cellular respiration converts sugar into ATP using oxygen.

Fig. S1. Summary of the altered metabolism pathways in alcoholic fatty liver disease using MetPA analysis (panel A).

Comprehensive and Easy Course Notes for BIOL1040 Exams and Assessment

BIOLOGY 103 Spring 2001 MIDTERM LAB SECTION

Receptor mediated Signal Transduction

Tutorial 27: Metabolism, Krebs Cycle and the Electron Transport Chain

Moderator Hamish Rodger ( Fish Vet Group )

Elevated carbon dioxide alters neural signaling and anti-predator behaviors in ocean phase coho salmon (Oncorhynchus kisutch)

T-cell activation T cells migrate to secondary lymphoid tissues where they interact with antigen, antigen-presenting cells, and other lymphocytes:

T-cell activation T cells migrate to secondary lymphoid tissues where they interact with antigen, antigen-presenting cells, and other lymphocytes:

Deciphering multiple stressor effects of gamma radiation and uranium on Atlantic salmon (Salmo salar): Transcriptomics-based mixture modeling

Cell Signaling part 2

SUPPLEMENTARY FIG. S2. b-galactosidase staining of

Table 1A. Genes enriched as over-expressed in DPM treatment group

ACTIVATION OF T LYMPHOCYTES AND CELL MEDIATED IMMUNITY

DIETARY LIPIDS, IMMUNE FUNCTION AND PATHOGENESIS OF DISEASE IN FISH

Lesson Overview. Cellular Respiration: An Overview. 9.2 process of cell respiration

Subject Index. Bcl-2, apoptosis regulation Bone marrow, polymorphonuclear neutrophil release 24, 26

Rho GTPase activating protein 8 /// PRR5- ARHGAP8 fusion

CYTOKINE RECEPTORS AND SIGNAL TRANSDUCTION

Supplementary Materials for

Allergy and Immunology Review Corner: Chapter 13 of Immunology IV: Clinical Applications in Health and Disease, by Joseph A. Bellanti, MD.

Enzyme-coupled Receptors. Cell-surface receptors 1. Ion-channel-coupled receptors 2. G-protein-coupled receptors 3. Enzyme-coupled receptors

Reduced dietary levels of EPA and DHA have a major impact on the composition of

Signaling Through Immune System Receptors (Ch. 7)

Principles of cell signaling Lecture 4

Quantidoc AS. The presence of health. Slimy Barriers: The first defense in disease

Trim29 gene-targeting strategy. (a) Genotyping of wildtype mice (+/+), Trim29 heterozygous mice (+/ ) and homozygous mice ( / ).

Supplementary data Table S3. GO terms, pathways and networks enriched among the significantly correlating genes using Tox-Profiler

BIOLOGY - CLUTCH CH.9 - RESPIRATION.

Contents The Cytokine System The Discovery of the Brain Immunomodulators

Osmoregulation and Osmotic Balance

Anatomy 36 Study Guide Unit 3

The unstable production of grouper fry in the hatchery is one of the constraints in the

Excretion. Consumption = Growth + (Metabolism + SDA) + F(egestion) + U (excretion) Energetics Processes. Hormonal Control

1. Cyanide is introduced into a culture of cells and is observed binding to a mitochondrion, as shown in the diagram below.

7 Pathways That Harvest Chemical Energy

Ben JG Sutherland 1, Kim W Koczka 1, Motoshige Yasuike 1,2, Stuart G Jantzen 1, Ryosuke Yazawa 1,3, Ben F Koop 1* and Simon RM Jones 1,4

UNDERSTANDING YOUR WATER PROFILE PRESENTED BY POULTRY PARTNERS AND AHPD

ACCESSORY PUBLICATION. Supplementary Table 1. Genes up regulated in the skin of both HR and LR animals. after tick larval challenge e

MCB 4211 Basic Immunology 2nd Exam; 10/26/17 Peoplesoft #:

4. Which step shows a split of one molecule into two smaller molecules? a. 2. d. 5

2. What is molecular oxygen directly converted into? a. Carbon Dioxide b. Water c. Glucose d. None of the Above

Chapter 11 CYTOKINES

Medical Virology Immunology. Dr. Sameer Naji, MB, BCh, PhD (UK) Head of Basic Medical Sciences Dept. Faculty of Medicine The Hashemite University

MULTIPLE CHOICE. Choose the one alternative that best completes the statement or answers the question.

CELLS. Cells. Basic unit of life (except virus)

Kortner et al. BMC Veterinary Research 2012, 8:101

Endocrine System Hormones. AP Biology

Biosecurity in Water Recirculation Aquaculture Systems. Christopher Good. Biosafety and Biocontainment Symposium Baltimore, Maryland February 6-9

Biology /08 Released Exam August 2008 Form A Provincial Examination Answer Key

Cellular Respiration and Fermentation

ulcer healing role 118 Bicarbonate, prostaglandins in duodenal cytoprotection 235, 236

Marah Bitar. Faisal Nimri ... Nafeth Abu Tarboosh

Nitrogen Metabolism. Overview

Immune Surveillance. Immune Surveillance. Immune Surveillance. Neutrophil granulocytes Macrophages. M-cells

Immune System AP SBI4UP

Enzymes and Metabolism

Cell Signaling (part 1)

Inflammatory Bowel Disease - Target Initial Survey

'Namgis First Nation's "KUTERRA" Project:

Chapter 14 - Electron Transport and Oxidative Phosphorylation

STEIN IN-TERM EXAM -- BIOLOGY FEBRUARY 15, PAGE

Chapter 13: Cytokines

Cell Cell Communication

Principles of Genetics and Molecular Biology

1. Cyanide is introduced into a culture of cells and is observed binding to a mitochondrion, as shown in the diagram below.

Supplementary information for: Community detection for networks with. unipartite and bipartite structure. Chang Chang 1, 2, Chao Tang 2

CELL BIOLOGY - CLUTCH CH CELL JUNCTIONS AND TISSUES.

NAME KEY ID # EXAM 3a BIOC 460. Wednesday April 10, Please include your name and ID# on each page. Limit your answers to the space provided!

Biology Ch 9 Cellular Respiration & Fermentation ( )

Macromolecules. You are what you eat! Chapter 5. AP Biology

Name: Bendik Fyhn Terjesen

Supplementary Material Correlation matrices on FP and FN profiles

Membrane Function. How does the cell membrane control movement of materials? Type 1 Ions Type 2 Molecules Type 3 Molecules Type 4 Molecules H O H

Published on Second Faculty of Medicine, Charles University ( )

Chemical Energy. Valencia College

9.2 The Process of Cellular Respiration

The Lymphatic System and Body Defenses

PSMD6_MOUSE 26S proteasome non-atpase regulatory subunit 6 D b AMMAKAEYL RT06_MOUSE 28S ribosomal protein S6, mitochondrial D b

STEIN IN-TERM EXAM -- BIOLOGY FEBRUARY 16, PAGE

KEY CONCEPT QUESTIONS IN SIGNAL TRANSDUCTION

GENERAL CHARACTERISTICS OF THE ENDOCRINE SYSTEM FIGURE 17.1

Transcription:

CO 2 tolerance of Atlantic salmon post-smolts in recirculating aquaculture systems Vasco Mota*, Tom Nilsen, Elizabeth Ytteborg, Grete Baeverfjord, Aleksei Krasnov, Jelena Kolarevic, Lars Ebbesson, Steven Summerfelt and Bendik Fyhn Terjesen * Norwegian Institute of Food, Fisheries and Aquaculture Research Nofima vasco.mota@nofima.no

Problem definition Fish exposed to high CO 2 shows: Lower: oxygen consumption, feed intake, growth Higher: Nephrocalcinosis (deposits in kidneys) & cataracts Impaired osmoregulation and blood acidosis Gene expression changes Dissolved CO 2 accumulates in RAS Recommendation for Atlantic salmon: 15 20 mg/l Atlantic salmon CO 2 tolerance in RAS is unknown

OBJECTIVE Carbon dioxide tolerance of Atlantic salmon post-smolts in RAS Foto: Aquafarm Equipment Vasco Mota - Overview of Atlantic salmon RAS and CtrlAQUA developments 21 August 2017 21

Experimental design

Experimental system Water quality Oxygen >85 % Salinity 12 ppt Temperature 12 13 C ph 6.7 7.7 Alkalinity 116 165 mg/l

R: health & welfare No observed effects on: haematocrit glucose hepatosomatic Index eye cataracts welfare score (skin & fins) nephrocalcinosis

Genes RAS 40 FTS 40 RAS 5 FTS 5 40R1>3100.diff_corr 40R2>3101.diff_corr 40R3>3102.diff_corr 40R4>3103.diff_corr 40R5>3104.diff_corr 40R6>3105.diff_corr 40S1>3106.diff_corr 40S2>3107.diff_corr 40S3>3108.diff_corr 40S4>3109.diff_corr 40S5>3110.diff_corr 40S6>3111.diff_corr 5R1>3112.diff_corr 5R2>3113.diff_corr 5R3>3114.diff_corr 5R4>3115.diff_corr 5R5>3116.diff_corr 5R6>3117.diff_corr 5S1>3118.diff_corr 5S2>3119.diff_corr 5S3>3120.diff_corr 5S4>3121.diff_corr 5S5>3122.diff_corr 5S6>3123.diff_corr Tpr protein - Ident 89-1.18-0.73-1.48-0.08-0.16-0.69 1.06 1.39 1.93 0.01 1.33-0.07 1.49 0.24 0.48-0.75 0.83 0.13-1.05-0.07-0.97-1.12-1.50 0.96 Ankycorbin -1.57-1.07-1.69-0.16-0.03-0.74 1.06 1.45 2.43 0.10 1.65-0.06 1.12-0.01 0.65-0.84 1.38 0.42-1.37-0.22-1.12-1.12-1.66 1.39 Dystonin -1.82-1.31-1.85 0.37 0.35-0.50 0.70 0.97 2.08-0.21 1.46 0.39 0.41-0.17 0.89-0.25 1.37 0.62-1.35-0.42-0.87-0.59-1.68 1.40 Tektin-4-1.04-1.09-0.98-0.46 0.09-0.31 0.49 0.87 1.40-0.56 1.76 0.24 0.76-0.35 0.04-0.78 1.70 0.47-0.87-0.36-0.78-1.06-0.64 1.47 Keratin_ type I cytoskeletal 18 - Ident 25-1.05-0.84-1.17-0.57-0.33-0.72 1.31 1.59 1.84-0.45 1.57-0.28 0.80-0.24-0.07-0.79 1.55-0.01-0.96 0.29-0.84-1.08-0.98 1.43 Tubulin--tyrosine ligase-like protein 12-0.84-0.75-1.05-0.36-0.11-0.49 0.78 0.97 1.43-0.25 1.12-0.08 1.50 0.04 0.10-0.77 0.92 0.15-0.77-0.19-0.85-0.95-0.35 0.81 Exosome complex exonuclease RRP4 [Salmo -1.11-0.62 salar] -2.08-0.06-0.25-1.15 1.67 1.92 2.09-0.23 1.32-0.16 2.01 0.47 0.60-1.27 1.04 0.02-0.81 0.50-1.31-1.45-2.10 0.98 Dexamethasone-induced Ras-related protein -0.49-0.51 1 precursor -0.26 [Salmo -0.46-0.12 salar] -0.44-0.25-0.51-0.05-0.41-0.59-0.04 0.37 0.11 0.89 1.00 1.45 1.26-0.11-0.45-0.16-0.23 0.20-0.21 Rho GTPase-activating protein 23-1.19-0.90-1.15-0.03-0.20-0.28 0.14 0.78 1.50-0.14 1.53 0.47 0.44-0.25 0.42-0.69 1.17 0.75-1.17-0.39-0.65-0.76-0.81 1.39 AP-1 complex subunit gamma-like 2 [Salmo -0.55 salar] -0.09-0.02 0.00 0.05-0.49-0.25 0.02 0.31 0.89 0.45 0.59 0.35 0.33 0.95-0.25 0.04 0.43-0.78-0.80-0.49-0.30-0.41 0.02 Diaphanous 2-1.40-1.07-1.49 0.15 0.17-0.36 0.53 0.81 1.96-0.05 1.70 0.26 0.99-0.43 0.37-0.80 1.38 0.50-1.26-0.51-0.82-1.05-0.97 1.39 Telomerase-binding protein EST1A -1.09-0.98-1.37-0.82-0.74-1.00 2.06 2.12 2.75-0.80 2.10-0.77 0.59-0.49-0.47-0.95 2.32-0.46-1.18 0.54-0.97-1.14-1.23 1.96 E3 ubiquitin-protein ligase MARCH4-1.42-0.93-2.24-0.46 0.40-0.34 1.13 1.33 1.73-0.40 1.70 0.53 1.52 0.02 0.37-0.88 1.51 0.73-1.23-0.27-1.48-1.40-1.41 1.51 MHC class I antigen [Salmo salar] 0.32 0.37-0.18-0.67 1.40-0.25 0.18 0.06-0.74 0.44 0.70 0.20 0.55-0.03 1.33-0.09-0.01 0.97-0.28-0.62-1.27-0.53-0.61-1.27 TAP2b [Salmo salar] -0.39-0.10 0.27-0.10-0.17-0.23-0.35-0.27-0.27-0.14-0.42-0.20 0.95 0.76 0.37 0.65 0.46 0.86-0.38-0.49-0.07-0.30-0.16-0.27 CC chemokine with stalk CK2 [Oncorhynchus -0.32-0.52 mykiss] 0.20 0.16-0.06-0.50-0.31 0.40-0.34-0.47-0.73-0.38 0.13 1.22 0.82 0.44 1.36 0.64-0.41 0.28-0.45-0.55-0.25-0.35 C-C motif chemokine 8 precursor [Salmo 0.30 salar] 0.00-0.30-0.71-0.15-0.17 0.12 0.35-0.12-0.71 0.09-0.31-0.01-0.36 1.36 0.89 1.52 1.07 0.25-0.26-0.40-0.36-1.15-0.94 C-X-C chemokine 2 1.32 1.31 0.61 0.40-0.02 0.92-0.48-0.26 0.14 0.83 1.37 0.19-0.89-0.61 0.62-0.80-0.78-0.77 1.08-0.75-0.72-0.73-1.40-0.57 C-X-C chemokine 9 1.33 1.30 0.58 0.45 0.03 0.93-0.45-0.24 0.15 0.85 1.14 0.24-0.76-0.56 0.64-0.89-0.82-0.91 1.09-0.73-0.78-0.75-1.38-0.48 Complement component C7 [Salmo salar] 1.42 1.27 0.73-0.14 0.50-0.27 0.66-0.46 0.49 0.37 0.15 0.62-0.32-0.72 0.09-0.63 0.02-0.92 1.22-0.83-0.86-0.75-1.41-0.21 Complement component C8 gamma chain 1.57precursor -0.65 1.09 [Salmo 0.97 salar] -0.13 1.47-0.46 0.36 0.76-0.22-0.59-0.15-0.15-0.37-0.80 0.18 0.16-0.66-0.51 0.03-0.65-0.22-0.15-0.87 SAPS domain family member 3 [Salmo-1.29 salar] -0.60-2.05 0.06-0.37-0.96 1.24 1.71 2.13 0.12 1.34-0.10 1.57 0.50 0.60-0.84 0.89 0.13-0.95 0.33-1.30-1.20-2.12 1.15 Small inducible cytokine A13 [Oncorhynchus -0.15 mykiss] 0.08 0.51-0.09-0.55-0.36-0.21-1.06-0.14 1.25-0.72-0.36-0.09 0.94 0.25 0.89 1.87 1.06-0.47-0.81-0.72-0.84 0.52-0.80 Small inducible cytokine A13 [Oncorhynchus -0.10 mykiss] 0.14 0.54-0.12-0.58-0.34-0.14-1.10-0.21 1.34-0.57-0.33-0.29 1.17 0.19 0.72 1.98 1.14-0.44-0.88-0.72-0.85 0.44-0.97 putative interferon-alpha/beta receptor 0.12 alpha -0.01 chain -0.32 [Oncorhynchus 0.19-0.61mykiss] 0.14-0.18 0.73 0.58 1.21 0.70 1.00 0.72-0.20-0.34 0.13-0.54-0.41-0.98-0.76-0.98 0.00 0.04-0.24 Complement component 9, Perforin-1-0.08 precursor 0.08 0.11-0.62 0.22 0.16-0.68 0.47-0.35-0.91-0.53-0.92 0.81 1.02 0.71 0.99 1.22-0.01-0.91 0.09-0.29-0.37-0.30 0.07 fish virus induced TRIM protein [Oncorhynchus -1.61-1.05 mykiss] -2.36-0.19 0.20-0.57 1.19 1.47 1.98-0.08 1.85 0.34 1.16 0.05 0.48-1.12 1.66 0.53-1.25 0.09-1.48-1.44-1.56 1.72 Gig2-0.30-0.59-0.67-0.47-0.45-0.48 0.25 0.00 0.19 0.08-0.12 0.12 1.45-0.39 0.59 0.43 0.72 0.51 0.35 0.08 0.12-0.47-0.35-0.61 Gig2-4 -0.55-0.57-0.11-0.55-0.66-0.91 0.85-0.51-0.02 0.45-0.46 1.37 0.88-0.78 0.28 0.54 0.52 0.91 0.15 0.12 0.65-0.15-0.66-0.79 Gig2-7 -0.35-0.83-0.61-0.67-0.58-0.63 0.50-0.09 0.19 0.11-0.20 0.80 1.42-0.61 0.58 0.51 0.79 0.86 0.33 0.01 0.26-0.53-0.44-0.81 ISG15-like -0.81-0.55-1.39 0.41-0.95-0.49 1.85-1.07-0.19 0.03-1.40 3.25 1.07-1.06 0.32 1.55 1.21 1.07-0.21-1.48 0.58 0.49-0.78-1.45 Ubiquitin protein ligase E3A, hect domain -0.32 0.36-0.17-0.54-0.29-0.70 0.46-0.37 0.47 0.25-0.06 1.45 0.53-0.18 0.60 0.35 0.13 1.02-0.14-0.73 0.08-0.53-0.74-0.90 ZNF 1.21 0.48-1.08-0.41-0.81-1.07 0.43-0.31 0.62 0.28-0.15 1.33 0.94-0.29 0.14 0.20 0.67 1.04 0.27-0.80 0.47-1.05-1.09-1.02 C-type lectin domain family 4 member 0.56 E [Salmo -0.89salar] -0.71 1.14-0.85-0.91-0.03 0.64 0.48 0.81-0.17-1.65 0.79 0.52 0.36 0.90 0.54 0.35-0.72-1.74 0.00-0.07-0.45 1.08 C-type lectin M4, cd209l 1.65 0.93 1.08-0.35 0.33 1.25 0.55 0.72 0.75 0.79 0.98-0.09-1.33-0.62 0.50-1.03-1.12-1.07 1.25-1.49-0.38-0.64-2.38-0.27 Fish-egg lectin [Salmo salar] -0.91 0.41-3.36-1.19-1.72-2.99 1.60 1.23 1.56 0.12 1.25 0.74 0.19-0.23 0.43-0.65-1.32 0.60 1.61 0.67 0.62-0.11 0.49 0.96 Rhamnose binding lectin STL2 [Oncorhynchus -0.86-1.44 mykiss] -2.59-0.37-1.13-0.49-0.11-1.50-1.18 2.02 0.82 0.78 1.39-0.68-0.76 0.35 0.86 1.83 0.89-0.98 2.17 0.91-0.26 0.32 Rhamnose-binding lectin precursor [Salmo -0.07 salar] -0.53-2.10-0.30-0.91-0.55 0.18-1.15-1.09 1.09 0.23-0.05 1.85-0.20-0.39 0.11 0.63 1.82 1.25-0.55 1.41 0.34-0.64-0.38 Growth factor independent 1.1-0.70-0.85-1.46-0.65 0.38-0.29 0.60 0.93 1.78-0.67 1.46 0.26 1.06-0.30 0.36-1.13 1.27 0.52-0.45-0.22-0.89-1.44-0.92 1.35 Matrix metalloproteinase-9 1.07-0.31 0.02 0.48-0.21 1.17-0.31-0.38-0.05-0.35 0.28 2.01-1.13-0.68-0.58-1.30-0.95-1.10 1.16 0.13 1.19-0.21-0.68 0.70 modified T cell receptor alpha [Salmo -0.70 salar] -0.59-0.93-0.56-0.06-0.54 0.87 1.17 1.22-0.58 1.22-0.15 1.53 0.04-0.17-0.72 1.19 0.11-0.80-0.02-0.87-0.80-0.93 1.04 TNF receptor member 11B -1.43-0.31-0.32-0.50 0.02-0.14 0.07-0.63-0.26 0.48 0.01-0.21 0.62 0.39 0.72 0.74 0.82 0.32 0.08-0.86 0.39 0.25 0.05-0.29 S100 calcium binding protein A10b -0.05-1.79-1.37-1.32-1.01-0.76 0.43 1.23 0.66 0.96 0.52-0.47-0.58-0.50-0.12-0.60-0.14 0.64 1.24 0.21 1.26 0.43 0.19 0.94 5-aminolevulinate synthase, erythroid-specific, 0.46 0.10mitochondrial -0.07-0.21 precursor 0.60-0.13 [Salmo -0.82 salar] -0.59-0.04 0.24-0.01-1.43-0.08-0.49 0.21 0.04-0.24 0.06 1.32 0.53-0.15 0.24-0.07 0.51 Cytidine deaminase [Salmo salar] 0.21-0.74-0.04 1.05-0.52 0.97 0.03-1.06-0.79 2.22 1.89 1.39-0.09-0.65-1.09-0.89-0.92-0.80 0.35-1.20-0.36-0.21 1.58-0.34 Ectonucleoside triphosphate diphosphohydrolase -1.21-0.59 2-1.02 [Salmo-0.57 salar] -0.38-0.80 1.44 1.79 1.99-0.39 1.79-0.29 0.57-0.28-0.12-0.82 1.65-0.19-1.08 0.21-1.06-1.11-0.93 1.40 Carboxypeptidase A1 precursor [Salmo 0.43 salar] -0.88-1.38-1.10-0.72-0.98-1.36-0.38 0.90 0.05 0.02 0.10 1.30 0.65 1.36 0.57-0.15 1.36-0.24 0.59-0.55-0.42 0.63 0.17 Nucleolar protein 5 [Salmo salar] 0.29-0.28 0.67-0.27 0.70-1.03 0.36-0.41-0.88-1.94 0.01 0.20-0.12 0.13-0.29 0.49 0.26-0.22-0.02 0.62 0.64 0.23 0.79 0.08 Poly polymerase 10 [Salmo salar] 0.42 0.33-0.16 0.55-0.04 0.90 0.22-1.05-1.09-0.27-1.00-1.12 0.23 0.50 0.40 0.08-0.02 0.12-0.29-0.08 0.53 0.54 0.52-0.23 Fatty aldehyde dehydrogenase [Salmo-1.52 salar] -1.17-1.50 0.69-0.15-0.48 0.06 0.70 2.65 0.48 1.39 0.29 1.01-0.19 1.28-0.80 0.91 0.46-1.32-1.58-0.48-0.57-1.50 1.35 Sulfotransferase family cytosolic 2B member -0.29 1.74 [Salmo -0.31 salar] -0.51-0.47-0.44-0.67-0.54-0.76-0.74-0.65-0.62-0.46-0.17 1.55 1.65-0.43-0.46 1.24-0.45 1.46 1.34-0.59-0.44 Claudin-like protein ZF4A22 [Salmo salar] -1.09-0.74-2.01-0.57 0.06-0.72 1.51 1.75 1.76-0.66 1.56 0.07 1.96 0.37 0.05-1.22 1.30 0.34-0.86 0.44-1.46-1.61-1.46 1.25 Fibulin-1 [Salmo salar] 0.88 0.13 0.66 0.34 0.02 0.96-0.08 0.30 0.40 0.13 0.02-0.18-0.80-0.71-0.63-0.44-0.19-0.26-0.10 0.24-0.12-0.06-0.22-0.27 Nephronectin variant 2 - Ident 84-1.82-0.76-2.01-0.51-0.45-1.23 1.91 2.20 2.07-0.66 1.96 0.32 1.26 0.30-0.16-1.12 1.92 0.37-0.93 0.40-1.66-1.38-1.70 1.68 Otoancorin -0.52-0.54-0.65-0.51-0.77-0.75 0.22 0.29 0.50 0.23 0.26-0.08 0.48-0.34 0.24 0.44 0.38 0.38 0.45 0.12 0.39 0.09-0.30 0.00 Glucocorticoid receptor -1.18-0.70-2.04-0.13-0.21-1.04 1.53 1.71 2.14-0.11 1.40-0.30 1.92 0.41 0.68-0.88 1.00 0.02-0.86 0.27-1.30-1.37-1.96 1.02 Inactive serine protease PAMR1-1.58-0.85-1.78 0.03-0.02-0.61 0.74 1.01 1.99 0.08 1.48 0.11 0.98 0.14 0.77-0.51 1.02 0.48-1.44-0.05-0.93-0.83-1.49 1.27 Solute carrier family 13 member 3, slsc13a -0.55-0.53-0.67-0.35-0.25-0.37 0.17 1.71 0.83-0.41 0.78 0.52 0.31-0.07-0.20-0.34 0.83-0.06-0.57-0.12-0.47-0.45-0.51 0.80 Tripartite motif-containing protein 55-1.60 [Salmo-1.56 salar] -1.93-0.05 0.33-0.30 0.71 0.61 2.57-0.34 1.88 0.46 1.04-1.21 0.82-0.68 1.71 0.49-1.25-0.39-0.63-1.32-0.96 1.59 homeobox protein HoxC8ba [Salmo salar] -1.45-0.99-1.66 0.32 0.05-0.63 0.75 0.96 2.15-0.03 1.59 0.09 1.28-0.12 0.56-0.64 1.17 0.36-1.23-0.32-1.00-0.99-1.49 1.26 Lim homeobox protein 3 [Salmo salar]-0.79-0.58-1.08-0.38-0.44-0.87 1.18 1.64 1.48-0.31 0.92-0.34 2.33 0.41-0.05-1.02 0.68-0.27-0.68 0.23-0.81-0.99-0.96 0.71 Extracellular matrix protein 1 precursor -1.02 [Salmo -1.33 salar] 0.20-1.09-1.70-0.72-0.03 0.10 1.38-1.09-0.22 0.62 0.41 0.25 0.41 1.89 0.68 1.85-1.45 1.24-0.41-0.29-0.30 0.62 leukolectin protein [Salmo salar] -0.62 0.39-3.07-1.34-1.79-2.98 1.75 1.27 1.52 0.06 1.04 0.45 0.18-0.12 0.50-0.98-1.30 0.46 1.83 0.61 0.96-0.13 0.36 0.92 leukolectin protein [Salmo salar] -0.89 0.47-3.38-1.20-1.72-3.23 1.73 1.25 1.67 0.04 1.14 0.61 0.27-0.26 0.53-0.90-1.39 0.48 1.69 0.83 0.85-0.15 0.53 1.02 Up-regulator of cell proliferation -0.07 0.47-0.39-0.46-0.55-0.75 0.00-0.15-0.45-0.21-0.36 0.61 1.43 0.72 0.77-0.27 1.04 0.13-0.75-1.11 0.26 0.23-0.38 0.22 Col6a2 protein - Ident 96 1.21 0.66-0.05 0.02-0.30 1.49-0.58-0.26 1.25 1.09 0.91-0.08 0.32 0.04 0.14-0.87-0.26-0.42 0.41-1.57-0.93-1.20-0.88-0.15 GMP Giant mucus protein -0.51 0.34-0.39-0.13 0.23-0.55 0.59 0.65 1.38 0.24 1.28 0.39 0.28-0.27 0.40-0.87 0.89-0.18-0.18-0.45-1.63-0.99-1.28 0.76 Calcitonin-1 precursor [Salmo salar] 0.09 1.12 0.51 0.60 0.69-0.22 0.23-0.65 0.21 0.47 0.47-0.21-0.23-0.56-0.16-0.65-1.27-0.33 0.41 0.13-0.24-0.31 0.14-0.22 growth hormone receptor isoform 1 precursor 0.16 0.91 [Salmo -0.07 salar] -0.34-0.17-0.08 0.50-0.15 0.29 0.85 1.04 0.54-1.16-0.72-0.38-0.38-1.05-0.71 0.69 0.41-0.25-0.12 0.27-0.07 Angiogenin-1 precursor / RNase ZF3 1.84-3.35-1.38-2.46-3.38-4.17-0.23 0.72 6.10-1.03-0.27-2.95 1.39-1.04 1.33 0.04 2.36 3.06 3.55-4.06 1.98-1.41-0.55 3.95 G-protein coupled receptor 183-0.76-0.61-0.76-0.19-0.02-0.28 0.41 0.82 1.21-0.43 1.27 0.14 0.58-0.25-0.06-0.64 1.10 0.21-0.70-0.27-0.56-0.83-0.49 1.10 UDP-GlcNAc:betaGal beta-1_3-n-acetylglucosaminyltransferase-like -1.09-0.60-1.96-0.25-0.351 --0.87 Ident 991.36 1.74 1.90-0.15 1.30-0.15 1.90 0.56 0.41-0.90 0.94 0.15-0.99 0.43-1.27-1.37-1.70 0.96 Optineurin [Salmo salar] -1.58-1.07-1.57 0.28-0.09-0.69 0.93 1.06 2.58 0.08 1.55-0.23 0.76-0.11 1.13-0.15 1.16 0.44-1.46-0.68-0.81-0.92-1.88 1.24 Synaptobrevin homolog ykt6 [Oncorhynchus -1.19-0.88 mykiss] -1.31 0.27-0.21-0.63 0.65 0.97 1.91 0.25 1.20-0.30 1.15-0.08 0.41-0.45 0.83 0.23-1.12-0.19-0.69-0.78-1.12 1.07 Synaptosomal-associated protein 25-A -1.81 [Salmo -1.04 salar] -2.01 0.45 0.12-0.61 0.38 1.10 2.15 0.56 1.63 0.28 0.76-0.09 0.73-0.74 1.42 0.53-1.49-0.15-1.09-0.74-1.75 1.43 Zymogen granule membrane protein -0.16 precursor 0.06 [Salmo -1.67 salar] 0.62-0.79-1.12-0.74-3.03-1.70 0.38 1.00 0.50 2.83 0.67 0.90 0.34 2.07 1.21 1.75-3.24 2.14-1.04-1.06 0.09 Zymogen granule membrane protein 16-0.70 precursor -1.52 [Salmo -0.17 salar] 0.79-0.74-1.64-0.06-2.77-1.94 0.49 0.17 0.74 2.86 2.13 1.75 1.00 1.88 0.65 1.39-1.59 1.18-0.14-3.28-0.49 Ceacam1-0.16-0.63-0.39-0.45-0.40 0.01-0.55-0.31-0.29-0.29 0.08 0.09 0.57 0.80 0.45 0.77 1.22 0.49-0.22-0.55 0.43-0.16-0.39-0.13 EGF-like domain-containing protein 7 precursor -1.64-0.75[Salmo -2.49salar] -0.39-0.25-1.04 1.99 2.22 2.19-0.33 1.93-0.03 1.25 0.29 0.30-1.16 1.86 0.15-1.17 0.67-1.77-1.58-2.02 1.77 Fibronectin 1b - Ident 28-0.21 0.58-0.27-0.80-0.27-0.58 0.01-0.09-0.40-0.31-0.12 0.64 1.34 0.65 0.68-0.18 0.85 0.10-0.62-1.05-0.14 0.18-0.34 0.35 Leucine rich repeat containing 8 family_ -0.08 member -0.25 C -0.38 0.08-0.22 0.12 0.52-0.20 0.42 1.60 0.54 0.45-1.19-0.53-1.27-0.90-1.08-0.61 0.29 0.35 0.73 0.41 1.02 0.18 Tetraspanin-1 [Salmo salar] -0.54-0.94-0.49-0.50-0.47-0.53-0.08-0.01-0.06 0.28 0.08-0.03 0.12 0.52-0.62 0.92 0.07 0.72 0.51-0.33 0.32 0.84 0.23 0.02 Uncharacterized protein -0.53-0.51 0.46-0.64 0.35-0.56 0.39-0.61-0.32-0.67-0.63-0.61-0.59 0.94 0.91 1.12 0.69 0.70 0.60 0.99 0.26-0.59-0.56-0.55 Uncharacterized protein 0.02-0.61 0.08-0.34-0.53-0.27 0.06-0.28-0.19-0.23-0.14 0.00 1.23 0.45 0.03-0.02 1.15 0.25-0.57-0.68 0.39 0.11-0.08 0.19 Uncharacterized protein -0.20 0.72-0.32-0.77-0.51-0.63-0.06 0.04-0.38-0.07-0.23 0.67 1.28 0.64 0.94-0.21 0.61 0.05-0.54-1.00-0.13 0.42-0.47 0.13 Unknown 0.23 0.68 0.09-1.11 1.34-1.32 0.06-0.88-1.11-1.29-0.98-0.04 0.92-1.25 0.91 0.79 0.22 0.32 0.96 0.24 0.82 0.78 1.01-1.41 Ankyrin repeat and SAM domain-containing -0.80 protein -0.50-1.41 6-0.32-0.37-0.82 1.35 1.62 1.64-0.40 0.93-0.48 2.31 0.49 0.08-0.89 0.59-0.07-0.67 0.34-1.02-1.04-1.24 0.67 Immunoglobulin superfamily member -0.55 8-0.80-0.26-0.46 0.62-0.32-0.51-0.55-0.36-0.14-0.32-0.25-0.83-0.62 0.46 0.80 0.64-0.50 0.63 0.81 0.99 0.83-0.11 0.82 R: gene expression microarray Gill: low DEG / gene suppression in RAS

R: physiology Negative correlation choride vs. bicarbonate Positive regression CO 2 plasma vs. water

R: fish growth

R: fish body weight

R: fish body weight

Conclusions no mortalities, cataracts, nephrocalcinosis or poor external welfare observed in fish exposed to CO 2 from 5 40 mg/l the highest non-observed effect concentration for several ions and growth was 5 mg/l Three take home messages Atlantic salmon very resilient to high CO 2 CO 2 has a growth penalty, and this penalty starts at lower concentrations than previously reported (<12 mg/l) early production conditions have a carry-over effect on fish performance at later phases

Special thanks to Michele Gallo, Jascha Gerwins, and all user partners, researchers, and technicians who have contributed. Thank you for your attention. Vasco Mota Contact: vasco.mota@nofima.no

2024 © healthdocbox.com Privacy Policy | Terms of Service | Feedback