Brzilin Journl of Medicl nd Biologicl Reserch (2001) 34: 1055-1064 Effect of exercise on glycolytic enzymes in fish tissues ISSN 0100-879X 1055 Influence of exercise on the ctivity nd the distriution etween free nd ound forms of glycolytic nd ssocited enzymes in tissues of horse mckerel V.I. Lushchk 1,*, T.V. Bgnyukov 1,*, J.M. Storey 2 nd K.B. Storey 2 1 Deprtment of Nturl Sciences, Precrpthin University, Ivno-Frnkivsk, Ukrine 2 Institute of Biochemistry nd Deprtment of Biology, Crleton University, Ottw, Ontrio, Cnd Correspondence V.I. Lushchk Deprtment of Nturl Sciences Precrpthin University 57 Shevchenko str. Ivno-Frnkivsk, 76000 Ukrine E-mil: lushchk@pu.if.u * On leve from Krdg Nturl Reserve, Ntionl Acdemy of Sciences of Ukrine, Kurortne, Feodosi, Crime, Ukrine Received April 17, 2000 Accepted My 15, 2001 Astrct Key words Trchurus mediterrneus Swimming exercise Glycolysis Sucellulr enzyme inding Introduction Brz J Med Biol Res 34(8) 2001
1056 V.I. Lushchk et l. Mteril nd Methods Experimentl nimls Biochemicl regents nd coupling enzymes Fish exercise Brz J Med Biol Res 34(8) 2001
Effect of exercise on glycolytic enzymes in fish tissues 1057 Preprtion of tissue extrcts Mesurement of enzyme ctivities Brz J Med Biol Res 34(8) 2001
1058 V.I. Lushchk et l. Tle 1. Effect of exercise on the ctivities (units/g wet weight) of glycolytic nd ssocited enzymes in white muscle of horse mckerel. Enzyme Control 5-min urst exercise 60-min cruiser exercise HK 0.28 ± 0.09 0.25 ± 0.06 0.17 ± 0.02 PGI 14.5 ± 0.4 15.6 ± 0.7 13.9 ± 0.2 PFK 0.76 ± 0.03 0.55 ± 0.08 0.36 ± 0.04, Ald 52.4 ± 8.7 54.3 ± 4.5 70.4 ± 7.1 PK 241 ± 24 112 ± 13 88.0 ± 7.7 LDH 140 ± 4 131 ± 17 121 ± 9 PGM 3.01 ± 0.16 2.41 ± 0.15 2.01 ± 0.32 FBPse 0.57 ± 0.04 0.43 ± 0.05 0.21 ± 0.01, Dt re reported s mens ± SEM of 3-4 determintions in tissue from seprte individuls. P<0.05 compred to the corresponding control vlue nd P<0.05 compred to the 5-min urst exercise group (two-tiled Dunnett test). HK, hexokinse; PGI, phosphoglucoisomerse; PFK, phosphofructokinse; Ald, ldolse; PK, pyruvte kinse; LDH, lctte dehydrogense; PGM, phosphoglucomutse; FBPse, fructose-1,6-isphosphtse. Tle 2. Effect of exercise on the ctivities (units/g wet weight) of glycolytic nd ssocited enzymes in red muscle of horse mckerel. Enzyme Control 5-min urst exercise 60-min cruiser exercise HK 0.42 ± 0.04 0.48 ± 0.03 0.39 ± 0.02 PGI 9.45 ± 0.93 6.23 ± 0.92 9.35 ± 0.36 PFK 0.17 ± 0.02 1.93 ± 0.61 0.20 ± 0.01 Ald 14.2 ± 1.2 11.4 ± 1.9 11.6 ± 1.6 PK 136 ± 13 122 ± 4 111 ± 4 LDH 31.7 ± 3.3 56.8 ± 5.6 36.7 ± 1.9 PGM 1.12 ± 0.04 1.12 ± 0.07 0.93 ± 0.05 FBPse 0.55 ± 0.12 0.23 ± 0.08 0.18 ± 0.01 Dt re reported s mens ± SEM of 3-4 determintions in tissue from seprte individuls. P<0.05 compred to the corresponding control vlue nd P<0.05 compred to the 5-min urst exercise group (two-tiled Dunnett test). For revitions, see legend to Tle 1. Results Brz J Med Biol Res 34(8) 2001
Effect of exercise on glycolytic enzymes in fish tissues 1059 Tle 3. Effect of exercise on the ctivity (units/g wet weight) of glycolytic nd ssocited enzymes in liver of horse mckerel. Enzyme Control 5-min urst exercise 60-min cruiser exercise HK 0.58 ± 0.07 0.40 ± 0.02 0.65 ± 0.15 PGI 10.8 ± 1.8 6.4 ± 0.6 8.1 ± 0.2 PFK 0.37 ± 0.32 0.32 ± 0.02 0.38 ± 0.09 Ald 2.65 ± 0.22 2.46 ± 0.15 2.34 ± 0.28 PK 6.9 ± 0.4 16.0 ± 1.7 8.0 ± 0.6 LDH 1.57 ± 0.37 1.64 ± 0.18 1.26 ± 0.08 PGM 3.12 ± 0.28 2.87 ± 0.37 2.31 ± 0.28 G6PDH 5.63 ± 0.60 4.45 ± 0.64 3.92 ± 0.04 FBPse 2.88 ± 0.26 2.43 ± 0.27 2.76 ± 0.19 Dt re reported s mens ± SEM of 3-4 determintions in tissue from seprte individuls. P<0.05 compred to the corresponding control vlue nd P<0.05 compred to the 5-min urst exercise group (two-tiled Dunnett test). G6PDH, glucose-6-phosphte dehydrogense. For other revitions, see legend to Tle 1. Tle 4. Effect of exercise on the ctivities (units/g wet weight) of glycolytic nd ssocited enzymes in rin of horse mckerel. Enzyme Control 5-min urst exercise 60-min cruiser exercise HK 0.21 ± 0.04 0.21 ± 0.01 0.18 ± 0.01 PGI 3.72 ± 0.11 3.97 ± 0.11 3.72 ± 0.11 PFK 0.18 ± 0.03 0.24 ± 0.03 0.25 ± 0.01 Ald 1.66 ± 0.11 2.69 ± 0.18 2.69 ± 0.12 PK 36.5 ± 1.8 34.5 ± 1.7 33.3 ± 1.2 LDH 9.20 ± 0.43 7.49 ± 0.36 8.24 ± 0.83 PGM 0.22 ± 0.06 0 0.07 ± 0.03 G6PDH 0.07 ± 0.01 0 0.08 ± 0.02 FBPse 0.05 ± 0.02 0 0 Dt re reported s mens ± SEM of 3-4 determintions in tissue from seprte individuls. P<0.05 compred to the corresponding control vlue (two-tiled Dunnett test). G6PDH, glucose-6-phosphte dehydrogense. For other revitions, see legend to Tle 1. Figure 1. Effect of exercise on the distriution of glycolytic nd 60 ssocited enzymes etween 50 free nd ound frctions in white muscle of horse mckerel. Control 40 (white rs), 5-min urst swimming (grey rs) nd 60- min cruiser swimming (lck 30 rs). Dt re reported s 20 mens ± SEM (N = 3-4 individul fishes). P<0.05 compred to the 10 corresponding control vlue nd P<0.05 compred to the 5-min 0 HK PGI PFK Ald PK LDH PGM FBP urst exercise group (two-tiled Dunnett test). HK, hexokinse; PGI, phosphoglucoisomerse; PFK, phosphofructokinse; Ald, ldolse; PK, pyruvte kinse; LDH, lctte dehydrogense; PGM, phosphoglucomutse; FBP, fructose-1,6-isphosphtse. Bound enzyme (%) Brz J Med Biol Res 34(8) 2001
1060 V.I. Lushchk et l. Figure 2. Effect of exercise on the distriution of enzymes etween free nd ound frctions in red muscle of horse mckerel. Other informtion s in the legend to Figure 1. Figure 3. Effect of exercise on the distriution of enzymes etween free nd ound frctions in liver of horse mckerel. G6PDH, glucose-6-phosphte dehydrogense. Other informtion s in the legend to Figure 1. Figure 4. Effect of exercise on the distriution of enzymes etween free nd ound frctions in rin of horse mckerel. Other informtion s in the legend to Figure 1. Bound enzyme (%) Bound enzyme (%) Bound enzyme (%) 60 50 40 30 20 10 70 60 50 40 30 20 10 0 100 90 80 70 60 50 40 30 20 10 0 0 HK PGI PFK Ald HK PGI PFK Ald PK LDH PGM FBP PK LDH PGM FBP G6PDH,,, HK PGI PFK Ald PK LDH Discussion Brz J Med Biol Res 34(8) 2001
Effect of exercise on glycolytic enzymes in fish tissues 1061 Brz J Med Biol Res 34(8) 2001
1062 Brz J Med Biol Res 34(8) 2001 V.I. Lushchk et l.
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