Effect Of MiCroPlex Chromium Methionine And Vitamin E Supplementation On Growth Performance And Immune Status Of Stressed Beef Calves

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Effect Of MiCroPlex Chromium Methionine And Vitmin E Supplementtion On Growth Performnce And Immune Sttus Of Stressed Beef Clves Z BCr - 16

Ojective Evlute MiCroPlex nd vitmin E effects on growth nd immune sttus of stressed feedlot clves during 28 d receiving period Z BCr - 17 MiCroPlex chromium methionine, mrketed outside North Americ s Avil Cr

Loction: Conditions Gnder Zett Feedlot Culicn, Sinlo, Mexico Reserchers: Dr L. Almeid Dr R. Brjs Universidd Autonom de Sinlo, Mexico Animls: Tretments: Durtion: 60 Drought-mster ull clves verge weight 306 l (139 kg) 1) Control 2) Control + 68 IU vitmin E/l DM (150 IU/kg) 3) Control + 1 ppm chromium from MiCroPlex 4) Control + 68 IU vitmin E/l DM (150 IU/kg) + 1.0 ppm chromium from MiCroPlex 28 d Z BCr - 18 MiCroPlex chromium methionine, mrketed outside North Americ s Avil Cr

Loction: Conditions Gnder Zett Feedlot Culicn, Sinlo, Mexico Reserchers: Dr L. Almeid Dr R. Brjs Universidd Autonom de Sinlo, Mexico Animls: Tretments: Durtion: 60 drought-mster ull clves verge weight Recommended 306 l (139 Feeding kg) Rte 225 to 500 l Clves: 0.75 g MiCroPlex 1000 / 100 l BW 1) Control 306 l Clves: 2.30 g MiCroPlex 2) Control + 68 IU vitmin E/l DM (150 IU/kg) 3) Control + 1 ppm chromium from MiCroPlex Tretment Feeding Rte 4) Control 1.0 ppm Cr-L-Met + 68 IU = vitmin 1.77 g MiCroPlex E/l DM 1000 (150 / 100 IU/kg) l BW + 1.0 ppm306 chromium l Clves: 5.41 from g MiCroPlex 28 d Z BCr - 18.1 MiCroPlex chromium methionine, mrketed outside North Americ s Avil Cr

Procedures Trnsported clves 206 miles (330 km) Dirt lots with utomtic wterers nd shde ccess Prior to initition of tretments, clves were fed lflf hy for 2 d upon rrivl t the feedlot Z BCr - 19

Clves processed dy 1 Procedures Vccinted for Clostridium nd Psteurell Implnted (Implemx Hoechst-Rousell) Dehorned Dewormed Blood smples collected on d 1, 7, 14 nd 28 Diets fed 2X/d Feed llotment djusted to permit smll excess over voluntry feed intke Z BCr - 20

Diets Ingredient, % s Fed Dys 1 to 14 Dys 15 to 28 Corn strw 35 28 Ground corn 16 25 Rice polishings 16 20 Cnol mel 16.3 11 Sugr cne molsses 14 13 Limestone 1.1 1.38 Ure 0.6 0.8 Minerl premix 1.0 0.8 Monensin 0.0 0.02 Z BCr - 21 Gnmin 2.5-18 (TMP; Gudljr, Mexico) Composition (mg/kg): I., 262.5; Se, 25.0; Co, 12.5; Cu, 500; Fe, 2500; Mn, 3750; Zn, 6250; P, 25,000; NCI, 375,000; CCo 3, 1,000,000 Rumensin, Elnco, Inc., Greenfield, IN

Chemicl Composition Of Diets Diets Item, DM Bsis Dys 1 to 14 Dys 15 to 28 Crude protein, % 14.1 13.7 NE m, Mcl/kg 1.42 1.57 NE m, Mcl/l 3.13 3.46 C, % 0.94 0.96 P, % 0.56 0.56 Z BCr - 22 Clculted nlysis using tulr vlues (NRC, 1996) nd vlues on the lel of premix

Effect Of MiCroPlex And Vitmin E On Serum IgG Dy Control Vitmin E MiCroPlex c Vitmin E d MiCroPlex + Effect y Serum IgG (mg/dl) 1 1910 1620 1620 1743 7 1708 1893 1728 1927 Vitmin E z 14 1860 2050 1860 2200 Vitmin E 28 2020 2032 1950 2347 Vitmin E Z BCr - 23 MiCroPlex chromium methionine, mrketed outside North Americ s Avil Cr 68 IU vitmin E/l (150 IU/kg) c 1.0 ppm chromium from MiCroPlex d 68 IU vitmin E/l (150 IU/kg) + 1.0 ppm Cr from MiCroPlex y Effects with P < 0.05 re reported z Vitmin E supplementtion effect, P = 0.04 Almeid nd Brjs, 1999. J. Anim. Sci. 77 (Suppl.1):218 (Astr.)

Effect Of MiCroPlex And Vitmin E On Serum IgM Dy Control Vitmin E MiCroPlex c Vitmin E d MiCroPlex + Effect y Serum IgM (mg/dl) 1 239 234 259 264 7 243 278 295 295 MiCroPlex z 14 253 287 315 328 MiCroPlex 28 241 251 269 264 Z BCr - 24 MiCroPlex chromium methionine, mrketed outside North Americ s Avil Cr 68 IU vitmin E/l (150 IU/kg) c 1.0 ppm chromium from MiCroPlex d 68 IU vitmin E/l (150 IU/kg) + 1.0 ppm Cr from MiCroPlex y Effects with P < 0.05 re reported z Chromium supplementtion effect, P = 0.01 Almeid nd Brjs, 1999. J. Anim. Sci. 77 (Suppl.1):218 (Astr.)

Serum IgM, mg/dl MEXICO Cr BEEF STUDY I Effect Of MiCroPlex z And Vitmin E On Serum IgM 335 Control Vit E MiCroPlex c MiCroPlex + Vit E d 315 295 275 255 235 215 1 7 14 28 Dys Z BCr - 24.1 MiCroPlex chromium methionine, mrketed outside North Americ s Avil Cr 68 IU vitmin E/l (150 IU/kg) c 1.0 ppm chromium from MiCroPlex d 68 IU vitmin E/l (150 IU/kg) + 1.0 ppm Cr from MiCroPlex z Chromium supplementtion effect dys 7 nd 14, P = 0.01 Almeid nd Brjs, 1999. J. Anim. Sci. 77 (Suppl.1):218 (Astr.)

Effect Of MiCroPlex And Vitmin E On Serum Asprtte Aminotrnsferse (AST) Dy Control Vitmin E MiCroPlex c Vitmin E d MiCroPlex + Effect x Serum AST, (U/L) 1 35.27 26.60 36.20 34.40 7 32.20 19.47 21.80 23.67 E X MiCroPlex y 14 22.80 20.60 21.00 20.00 28 29.93 20.93 15.46 23.06 MiCroPlex z Z BCr - 25 MiCroPlex chromium methionine, mrketed outside North Americ s Avil Cr 68 IU vitmin E/l (150 IU/kg) c 1.0 ppm chromium from MiCroPlex d 68 IU vitmin E/l (150 IU/kg) + 1.0 ppm Cr from MiCroPlex x Effects with P < 0.05 re reported y Vitmin E X chromium interction, P = 0.01 z Chromium supplementtion effect, P < 0.01 Almeid nd Brjs, 1999. J. Anim. Sci. 77 (Suppl.1):218 (Astr.)

Serum AST, U/L MEXICO Cr BEEF STUDY I Effect Of MiCroPlex And Vitmin E On Serum Asprtte Aminotrnsferse (AST) yz 40 Control Vit E MiCroPlex c MiCroPlex + Vit E d 35 30 25 20 15 1 7 14 28 Dys Z BCr - 25.1 MiCroPlex chromium methionine, mrketed outside North Americ s Avil Cr 68 IU vitmin E/l (150 IU/kg) c 1.0 ppm chromium from MiCroPlex d 68 IU vitmin E/l (150 IU/kg) + 1.0 ppm Cr from MiCroPlex y Vitmin E X chromium interction d 7, P = 0.01 z Chromium supplementtion effect d 28, P < 0.01 Almeid nd Brjs, 1999. J. Anim. Sci. 77 (Suppl.1):218 (Astr.)

Effect Of Feeding MiCroPlex And Vitmin E To Stressed Clves GROWTH PERFORMANCE Item Control Vitmin E MiCroPlex c Vitmin E d MiCroPlex + Initil wt, l 300.5 298.2 298.7 293.4 Finl wt, l 360.4 358.9 362.4 367.5 Effect w ADG, l 2.1 2.2 2.6 2.6 MiCroPlex x DM intke, l/d 12.2 11.1 11.8 12.0 Feed/gin 5.68 5.13 4.56 4.57 MiCroPlex y, E X MiCroPlex z Z BCr - 26 MiCroPlex chromium methionine, mrketed outside North Americ s Avil Cr 68 IU vitmin E/l (150 IU/kg) c 1.0 ppm chromium from MiCroPlex d 68 IU vitmin E/l (150 IU/kg) + 1.0 ppm Cr from MiCroPlex w Effects with P < 0.05 re reported x Chromium supplementtion effect, P < 0.01 y Chromium supplementtion effect, P = 0.04 z Vitmin E X chromium interction, P = 0.03 Brjs nd Almeid, 1999. J. Anim. Sci. 77 (Suppl.1):269 (Astr.)

Effect Of Feeding MiCroPlex And Vitmin E To Stressed Clves GROWTH PERFORMANCE Item Control Vitmin E MiCroPlex c Vitmin E d MiCroPlex + Initil wt, kg 136.2 135.3 135.5 133.1 Finl wt, kg 163.5 162.8 164.4 166.7 Effect w ADG, kg 0.97 0.98 1.18 1.20 MiCroPlex x DM intke, kg/d 5.51 5.04 5.37 5.45 Feed/gin 5.68 5.13 4.56 4.57 MiCroPlex y, E X MiCroPlex z Z BCr - 27 MiCroPlex chromium methionine, mrketed outside North Americ s Avil Cr 68 IU vitmin E/l (150 IU/kg) c 1.0 ppm chromium from MiCroPlex d 68 IU vitmin E/l (150 IU/kg) + 1.0 ppm Cr from MiCroPlex w Effects with P < 0.05 re reported x Chromium supplementtion effect, P < 0.01 y Chromium supplementtion effect, P = 0.04 z Vitmin E X chromium interction, P = 0.03 Brjs nd Almeid, 1999. J. Anim. Sci. 77 (Suppl.1):269 (Astr.)

Effect Of Feeding MiCroPlex And 6 5 4 3 2 1 Vitmin E To Stressed Clves 2.1 2.2 2.6 MEXICO Cr BEEF STUDY I GROWTH PERFORMANCE 2.7 5.7 5.1 4.6 4.6 Control Vit E MiCroPlex c MiCroPlex + Vit E d 0 Averge Dily Gin x, l Feed/Gin yz Z BCr - 27.1 MiCroPlex chromium methionine, mrketed outside North Americ s Avil Cr 68 IU vitmin E/l (150 IU/kg) c 1.0 ppm chromium from MiCroPlex d 68 IU vitmin E/l (150 IU/kg) + 1.0 ppm Cr from MiCroPlex x Chromium supplementtion effect, P < 0.01 y Chromium supplementtion effect, P = 0.04 z Vitmin E X chromium interction, P = 0.03 Brjs nd Almeid, 1999. J. Anim. Sci. 77 (Suppl.1):269 (Astr.)

1.5 Effect Of Feeding MiCroPlex And Vitmin E To Stressed Clves GROWTH PERFORMANCE 1.18 1.20 1.0 0.97 0.98 0.5 Z BCr - 27.2 0.0 Control Vit E MiCroPlex c MiCroPlex + Vit E d Averge Dily Gin z, kg MiCroPlex chromium methionine, mrketed outside North Americ s Avil Cr 68 IU vitmin E/l (150 IU/kg) c 1.0 ppm chromium from MiCroPlex d 68 IU vitmin E/l (150 IU/kg) + 1.0 ppm Cr from MiCroPlex z Chromium supplementtion effect, P < 0.01 Brjs nd Almeid, 1999. J. Anim. Sci. 77 (Suppl.1):269 (Astr.)

6 5 Effect Of Feeding MiCroPlex And Vitmin E To Stressed Clves FEED CONVERSION 5.7 5.1 4.6 4.6 4 3 2 1 Control Vit E MiCroPlex c MiCroPlex + Vit E d Z BCr - 27.3 Feed/Gin z MiCroPlex chromium methionine, mrketed outside North Americ s Avil Cr 68 IU vitmin E/l (150 IU/kg) c 1.0 ppm chromium from MiCroPlex d 68 IU vitmin E/l (150 IU/kg) + 1.0 ppm Cr from MiCroPlex y Chromium supplementtion effect, P = 0.04 z Vitmin E X chromium interction, P = 0.03 Brjs nd Almeid, 1999. J. Anim. Sci. 77 (Suppl.1):269 (Astr.)

Conclusions Newly rrived feedlot clves responded to tretments Vitmin E Incresed IgG levels MiCroPlex Incresed serum IgM levels Decresed serum sprtte minotrnsferse levels indicting reduction in stress Improved verge dily gin Better feed conversion Z BCr - 28 MiCroPlex chromium methionine, mrketed outside North Americ s Avil Cr

Impliction Feeding MiCroPlex during the receiving period reduces negtive stress effects nd improves performnce Z BCr - 29 MiCroPlex chromium methionine, mrketed outside North Americ s Avil Cr