2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Issue date: 17 August 2016 Worcestershire Royal Hospital Contact: Peter Jackson Microbiology Laboratory Tel: +44 (0) 1905 760192 Ext 30659 Charles Hastings Way E-Mail: Peter.JACKSON1@worcsacute.nhs.uk Worcester Website: http://www.worcsacute.nhs.uk/ WR5 1DD Testing performed at the above address only DETAIL OF ACCREDITATION AND TISSUES Aspirates Cellular and fluid material contained on swabs, cannulae, Fluids Genital samples Pus Samples from sterile sites Semen Tissues Urine Whole blood Urine clinical diagnosis General isolation of microorganisms of clinical significance General isolation of microorganisms of clinical significance Quantification of white and red blood cells, bacteria and epithelial cells Manual or automated (biomerieux Previ Isola) inoculation followed by culture using SOPs: WI-U-MIC-Gramstain LP-U-MIC-Abscess LP-W-MIC-Bordetella LP-U-MIC-EarSwabs LP-U-MIC-EyeSwabs LP-U-MIC-FaecProc LP-U-MIC-Genitals LP-W-MIC-NoseSwabs LP-U-MIC-OsteoTissue LP-U-MIC-ProstJointsProc LP-U-MIC-Routinespits LP-U-MIC-Sterilefluids LP-U-MIC-SupWounds LP-U-MIC-Throatswabs LP-W-MIC-Tips (intravascular cannulae) LP-U-MIC-TissBiopsy LP-U-MIC-Urines LP-U-MIC-Previ Automated culture using BacTalert and; LP-U-MIC-BCPos LP-U-MIC-BactalertProc Automated urine analysis using UF1000: LP-U-MIC-UF1000iProc Assessment Manager: DE Page 1 of 6
Cerebral fluid samples Quantification of white and red blood cells and bacteria Microscopy (inc. Gram stain) using SOP: LP-U-MIC-CSF (CSF) Swabs Isolation of MRSA Manual or automated culture using: LP-U-MIC- MRSACultScreens Biomerieux Previ Isola (Inoculation) LP-U-MIC-Previ (MRSA screening swabs) Respiratory samples Urine CSF Skin, Nail Isolation and presumptive identification of Acid-alcohol fast bacilli (inc. Mycobacterium spp.) General isolation and characterisation of clinically significant fungi Identification of clinically significant bacteria Antimicrobial susceptibility testing of clinically significant bacteria Automated culture and microscopy (screening by fluorescent microscopy using Auramine stain, and ZN light microscopy on automated detection) using BacTalert and; LP-U-MIC-BactalertProc LP-U-MIC-TBculture WI-U-MIC-AuramineTB WI-U-MIC-ZNStain Manual culture and fluorescent microscopy using calcofluor stain using SOP: LP-U-MIC-Mycology Automated Matrix-assisted laser desorption/ionisation (MALDITOF) using: LP-U-MIC-VitekMSProc Biochemical profile determination using: SOP- LP-U-MIC-Vitek2Proc Manual disc diffusion and/or automated methods using BSAC methodology: SOP- LP-U-MIC-DiscTestingMethod SOP- LP-U-MIC-Vitek2Proc Gradient MIC determination by E-test LP-U-MIC-Etestprocedure Assessment Manager: DE Page 2 of 6
Identification of Staphylococcal isolates Serological identification of groups A, B, C, D, F and G of the Lancefield groups of Streptococci grown on agar plates Detection of Salmonella polyvalent 'O' (PSO) and Salmonella polyvalent 'H' (PSH); Shigella species; Vibrio species. Identification of E. coli O157 Identification of Campylobacter species and Yersinia species Detection of ova, cysts and parasites (OCP) Detection of Cryptosporidium oocysts Detection of C. difficile GDH antigen Detection of C. difficile toxin A & B Prolex staph latex SOP-LP-U-MIC-StaphID Pro-Lab Strep grouping kit SOP-LP-U-MICStrepID MAST agglutination using SOP-LP-U-MIC-Sal/ShigID LP-U-MIC-VibrioID E.col O157 Oxoid Latex Test using: LP-W-MIC-0157ID Light microscopy and Gram stain using: LP-U-MIC-CampID LP-W-MIC-YersID Concentration and light microscopic examination using Parasep method: LP-U-MIC-EndoParasiteProc Fluorescent microscopy (Auramine stain) using: LP-U-MIC-EndoParasiteProc WI-U-MIC-CryptoStain Launch/Alere DS2 (EIA): LP-U-MIC-GDHEIA LP-U-MIC-DS2 Launch/Alere DS2 (EIA): LP-U-MIC-CDToxinABEIA Assessment Manager: DE Page 3 of 6
Detection of Rotavirus Launch/Alere DS2 (EIA): LP-U-MIC-RotavirusEIA Plasma Serum Detection of Helicobacter pylori antigen Serological activities for the purpose of clinical diagnosis CMV detection EBV Serology Hepatitis A detection Hepatitis B detection HIV detection and confirmation Mumps Serology Rubella detection Toxoplasma detection Launch/Alere DS2 (EIA): LP-U-MIC-HPSAg where relevant Using Biomerieux VIDAS30 (EIA): LP-U-MIC-CMVIgGVIDAS LP-U-MIC-CMVIgMVIDAS LP-U-MIC-EBVVIDASProc LP-U-MIC-HepAIgGVIDAS LP-U-MIC-HepAIgMVIDAS LP-U-MIC-HBsAgVIDAS LP-U-MIC-HBcAbVIDAS LP-U-MIC-HBsAbVIDAS LP-U-MIC-HIV12AgABVIDAS and Alere determine LP-U-MIC-HIV1&2Confirmation Manual test LP-U-MIC-MumpsIgG LP-U-MIC-RubellaIgGVIDAS LP-U-MIC-RubellaIgMVIDAS LP-W-MIC-ToxoVIDAS Assessment Manager: DE Page 4 of 6
Plasma Serum Cultures isolated inhouse Respiratory samples Serum Serological activities for the purpose of clinical diagnosis (cont d) Measles Serology VZV serology Erythrovirus serology Syphilis Serology Detection of latent TB Anti-streptococcal serology Molecular examination clinical diagnosis Molecular detection of MRSA Molecular detection of Influenza A & B Molecular detection of HSV where relevant Using Biomerieux VIDAS30 (EIA): Automated ELISA using DS2 analyser and EIA using Biomerieux VIDAS30: LP-U-MIC-MeaslesIgG Automated ELISA using DS2 analyser and EIA using Biomerieux VIDAS30: LP-U-MIC-VZVIgGEIA LP-U-MIC-VZVIgGVIDAS DS2 analyser: LP-U-MIC-ErythrovirusEIA DS2 analyser: LP-U-MIC-TreponemaPallidumEIA DS2 analyser: LP-U-MIC-Quantiferon-TB Gold Biokit latex slide method: LP-U-MIC-ASOT LP-U-MIC-ASD where relevant: MagnaLyser extraction and Roche LightCycler 2.0 LP-U-MIC-MRSAPCR Biomerieux Easymag extraction platform and Roche LightCycler 2.0 LP-U-MIC-easyMAG LP-U-MIC-FLU PCR Roche cobas4800 platform and Roche LightCycler 2.0 LP-U-MIC-HSVPCR Assessment Manager: DE Page 5 of 6
Endocervical swabs, vaginal swabs, male/female urine, vulval swabs, urethral swabs Molecular examination Detection of Chlamydia trachomatis and Neisseria gonnorrhoeae DNA Detection of C. difficile toxin A & B where relevant: Roche COBAS4800 Nucleic acid amplification: LP-U-MIC-COBAS4800 and Confirmation using Cepheid GeneXpert LP-U-MIC-CepheidCT&GCPCR Confirmation on Cepheid GeneXpert: LP-U-MIC-CDIFFPCR Detection of Norovirus Biomerieux Easymag extraction platform and Roche LightCycler 2.0: LP-U-MIC-NorovirusPCR Serum HIV viral load Roche Ampliprep: LP-U-MIC-AmpliprepTaqman LP-U-MIC-HIVViralLoad Serum HCV Quantitative Roche Ampliprep: LP-U-MIC-AmpliprepTaqman LP-U-MIC-HCVPCR END Assessment Manager: DE Page 6 of 6