Dayan Knox Curriculum Vitae

Transcription

1 Research Interests Peripheral hormones (e.g. glucocorticoids) and central ascending arousal systems (e.g. locus coeruleus efferents) contribute to cognitive/emotional processes that are critical for normal psychological functioning. These systems are also sensitive to stress, and under certain circumstances, may contribute to abnormal cognitive/emotional processes that result in psychiatric disorders. My research interests concerns exploring how stress-induced changes in peripheral hormonal and ascending arousal systems contribute to abnormal cognitive/emotional processes. Employment 09/12 3/12 7/12 10/05 02/12 Education 09/03 09/05 09/01 08/03 09/97 05/01 Assistant professor in Psychological and Brain Sciences at The University of Delaware Research faculty in the Psychiatry Department at the University of Michigan Post doctorate fellow in the Psychiatry Department at the University of Michigan Ph.D. in Psychology, The Ohio State University Master s in Psychology, The Ohio State University Undergraduate major in Psychology at North Carolina Central University Research Funding and Awards Title: The roles of glucocorticoid receptors and phosphoinositol-3 kinase signaling in mediating extinction memory deficits in the SPS model. Award number: 1P20GM Granting Agency: NIH/COBRE Description of award: The goal of this project is to demonstrate that stress exposure can have dualistic effects, with some neurobiological effects being adaptive and other effects being maladaptive. Role: Principal Investigator. Award period: 1/16/15 5/30/17 Title: Traumatic stress exposure induces long-term extinction deficits by enhancing glucocorticoid receptor interactions with kinases in the ventral hippocampus Granting Agency: University of Delaware Research Foundation Description of award: This is a private foundation at the University of Delaware that awards funding to young research investigators in the natural sciences on a competitive basis. Role: Principal Investigator. 1

2 Award period: 6/1/14 5/31/16 Title: Medial Prefrontal Cortex And HPA Axis Roles In Generation Of PTSD-Like, Symptoms, In SPS Model (W81XWH ) Granting Agency: Department of Defense Description of award: This was an RFP for basic science research examining the neurobiology of PTSD. Funding levels for this RFP was estimated to be at 3 % of total grant applications received by the Department of Defense. Role: Co-Investigator. Was responsible for conducting background research (e.g. Pubmed searches), designed and performed experiments that contributed to the preliminary data set, and wrote drafts of the grant application. Award period: 9/1/08 9/30/12 Publication Record 1. Knox, D. and Keller, S.M. (2015). Cholinergic neuronal lesions in the medial septum and vertical limb of the Diagonal Bands of Broca induce contextual fear memory generalization and impair acquisition of fear extinction. Hippocampus (in press). 2. Keller, S.M., Schreiber, W.B., Stanfield, B.R., Knox, D. (2015). Inhibiting corticosterone synthesis during fear memory formation exacerbates cued fear extinction memory deficits within the single prolonged stress model. Behavioural Brain Research 287: Keller, S.M., Schreiber, W.B., Staib, J.S., Knox, D. (2015). Sex differences in the single prolonged stress model. Behavioural Brain Research 286: George, S.A., Stout, S., Tan, M., Knox, D., Liberzon, I. (2013). Early handling attenuates enhancement of glucocorticoid receptors in the prefrontal cortex in an animal model of post-traumatic stress disorder. Biology of Mood and Anxiety Disorders 3(1): Eagle, A., Knox, D., Roberts, M.M., Mulo, K., Liberzon, I., Galloway, M.P., and Perrine, S.A. (2013). Single prolonged stress enhances hippocampal glucocorticoid receptor and phosphorylated protein kinase B levels. Neuroscience Research 75, George, S.A., Knox, D., Curtis, A., Aldridge, J.W., Valentino, R., and Liberzon, I. (2013). Altered noradrenergic activity in an animal model of PTSD. European Journal of Neuroscience 37, Knox, D., Nault, T., Henderson, C., and Liberzon, I. (2012). Glucocorticoid Receptors And Extinction Retention Deficits In The Single Prolonged Stress Model. Neuroscience, 223: Knox, D., Fitzpatrick, C.J., George, S.A., Abelson, J.A., and Liberzon, I. (2012). Unconditioned fear is enhanced in an appetitive context. Neurobiology of Learning and Memory: (97)4: Knox, D., George, S.A., Fitzpatrick, C.J., Rabinak, C., Maren, S., and Liberzon, I. (2012). Single prolonged stress disrupts retention of extinguished fear in rats. Learning & Memory, 19(2):

3 10. Liberzon, I and Knox, D. (2012). Expanding Our Understanding Of Neurobiological Mechanisms Of Resilience By Using Animal Models. Neuropsychopharmacology, 37(2): Fitzpatrick, C., Knox, D., and Liberzon, I. (2011). Inactivation of the prelimbic cortex enhances TMT-induced freezing. Behavioural Brain Research: 221(1): Knox, D., Perrine, S, George, S., Galloway, M., and Liberzon, I. (2010). Single prolonged stress decreases glutamate, glutamine, and creatine concentrations in the rat medial prefrontal cortex. Neuroscience Letters, 480(1): Knox, D. and Berntson, G. G. (2008). Cortical modulation by nucleus basalis magnocellularis corticopetal cholinergic neurons during anxiety-like states are reflected by decreases in delta. Brain Research 1227: Knox, D., Holly B., Norman, J. G., and Berntson G. G., (2008). Nucleus basalis magnocellularis and substantia innominata corticopetal cholinergic lesions attenuate freezing induced by predator odor. Behavioral Neuroscience 122(3): Knox, D., and Berntson, G. G., (2006). Effect of nucleus basalis magnocellularis cholinergic lesions on fear-like and anxiety-like behavior. Behavioral Neuroscience 120(2): Lorzano, D.L., Norman, J. G., Knox, D., Wood, B. L., Miller, B. D., Emery, C. F., and Berntson, G. G. (2006). Where to B in dz/dt. Psychophysiology 44(1): Knox, D., Berntson, G. G., and Sarter, M. (2004). Visceral afferent bias on cortical processing: role of adrenergic afferents to the basal forebrain cholinergic system. Behavioral Neuroscience 118(6): Berntson, G. G., Shafi, R., Knox, D. and Sarter, M. (2003). Blockade of epinephrine priming of the cerebral auditory evoked response by cortical cholinergic deafferentation. Neuroscience 116: Impact of select publications Knox, D., George, S.A., Fitzpatrick, C.J., Rabinak, C., Maren, S., and Liberzon, I. (2012). Single prolonged stress disrupts retention of extinguished fear in rats. Learning & Memory, 19(2): Times cited: 30 Select citations 1) Andero, R. (2015). "Nociceptin and the nociceptin receptor in learning and memory." Progress in Neuro-Psychopharmacology & Biological Psychiatry 62: ) Bowers, M. E. and K. J. Ressler (2015). "An Overview of Translationally Informed Treatments for Posttraumatic Stress Disorder: Animal Models of Pavlovian Fear Conditioning to Human Clinical Trials." Biological Psychiatry 78(5): E15-E27. 3) Enman, N. M., K. Arthur, S. J. Ward, S. A. Perrine and E. M. Unterwald (2015). "Anhedonia, Reduced Cocaine Reward, and Dopamine Dysfunction 3

4 in a Rat Model of Posttraumatic Stress Disorder." Biological Psychiatry 78(12): Knox, D., Perrine, S, George, S., Galloway, M., and Liberzon, I. (2010). Single prolonged stress decreases glutamate, glutamine, and creatine concentrations in the rat medial prefrontal cortex. Neuroscience Letters, 480(1): Time cited: 29 Select citations 1) Animal models in translational studies of PTSD. (2013). Nikolaos DP et al. Neuroendocrinology 38(9): ) Ketamine, magnesium and major depression - From pharmacology to pathophysiology and back. (2013). Harald, M. Journal of Psychiatric Research 47(7): ) Creatine metabolism and psychiatric disorders: Does creatine supplementation have therapeutic value? (2012). Patricia, AJ. Neuroscience & Biobehavioral Reviews 36(5): Knox, D., and Berntson, G. G., (2006). Effect of Nucleus Basalis Magnocellularis Cholinergic Lesions on Fear-like and Anxiety-like Behavior. Behavioral Neuroscience 120(2): Times cited: 16 Select citations: 1) Recent Progress in Research on Ribosome Inactivating Proteins. (2010). Ng TB, Wong JH, Wang HX. Current Protein & Peptide Science, Vol. 11(1): ) Behavioral and hormonal reactivity to threat: Effects of selective amygdala, hippocampal or orbital frontal lesions in monkeys. (2008). Machado CJ, Bachevalier. Psychoneuroendocrinology, Vol. 33(7): ) The role of the nucleus basalis magnocellularis in fear conditioning consolidation in the rat. (2007). Baldi E, Mariottini C, Bucherelli. Learning & Memory, Vol. 14(2): Teaching Experience Teaching in a classroom setting University of Delaware: Spring 2016 NSCI320, Introduction to Neuroscience PSYC 667, Neurobiology of memory Spring 2015 NSCI320, Introduction to Neuroscience NSCI320, Introduction to Neuroscience (honors) Spring 2014 NSCI320, Introduction to Neuroscience 4

5 Ohio State University: Fall 2003 Winter 2005 Psych 100, Introduction to Psychology (in the winter and fall quarters) Mentoring Experience University of Delaware, Department of Psychology Time period: Fall present Description: As an Assistant Professor at the University of Delaware I am responsible for organizing research activity in my laboratory. My responsibilities involve interviewing graduate and undergraduate students for the lab, teaching students molecular and behavioral laboratory techniques, assigning grades for students, and supervising all research projects. In total, I currently have one incoming graduate student and six undergraduate students in my laboratory. Some of my students and their current positions are listed below. Student Role of mentee Current position Jennifer Staib Summer Scholar, Senior thesis Lab manager, undergraduate researcher Briana Stanfield Senior thesis Graduate student in the neuroscience program at Rutgers University Newark Thomas DiPietro Summer Scholar Undergraduate researcher Caroline Lawless 4+1 Master s program Incoming Masters Student University of Michigan Medical School Time period: Summer/2006 Summer 2012 Description: As a postdoctoral fellow and research faculty at the University of Michigan I was responsible for organizing undergraduate research activities in the laboratory of Dr. Israel Liberzon and served as direct supervisor for many undergraduate students. My responsibilities involved interviewing students for the lab, teaching students molecular and behavioral laboratory techniques, recommending grades for students, and helping students with writing assignments. I also helped design experiments that certain students used for their senior and honor theses. In total, I have acted as direct supervisor for 12 students and have served as direct supervisor for the following senior and honor theses. Student Role of Mentor Position immediately after working in the lab Christopher J. Fitzpatrick, B.S. in Brain, Cognition, and Behavior Directly supervised research in the lab Graduate student in the Neuroscience program at the University of Michigan Stephanie Stout, B.S. in Brain, Directly supervised research Graduate student in the 5

6 Cognition, and Behavior in the lab Neuroscience program at the University of Denver Melissa Tan, B.S. in Neuroscience Tori Nault, B.S. in Neuroscience Directly supervised senior thesis (honors) Directly supervised senior thesis (honors) Medical student at Duke University Medical student at Oakland Medical School MI. Invited talks 9/15 12/14 9/13 5/13 3/13 12/12 09/12 The effects of traumatic stress on fear extinction. Pavlovian Society, Portland OR The role of glucocorticoid receptors and PI3K signaling in mediating extinction retention deficits in the SPS model. COBRE external committee meeting, DBI, Newark DE. Animal models of fear and extinction learning. Social Psychology Brown Bag Series. Department of Psychology, University of Delaware, Newark DE. The role of central arousal systems in fear memory processes. Research talk. Laboratory of Dr. Rita Valentino at the University of Pennsylvania, Philadelphia PA. Secondary characteristics of fear memory. Biology seminar. Department of Biology, Delaware State University, Dover DE. Neurobiological mechanisms by which fear memory persists in PTSD. Behavioral Neuroscience Brownbag Series. Department of Psychology, University of. Establishing research collaborations between the Veterans Affairs Hospital at Coatesville and the Department of Psychology at the University of Delaware. Research collaboration initiative. Veterans Affairs Hospital, Coatesville PA Recent Conference Proceedings Knox, D., Stanfield, BS., Staib., JM., Keller, SM., DiPietro, T. Society for Neuroscience, Chicago IL. Using measures of transcriptional activity to determine mnemonic processes through which SPS exposure leads to extinction retention deficits. Knox, D., Stanfield, BS., Staib., JM., Keller, SM., DiPietro, T. Pavlovian Society, Portland OR. Using Measures of c-jun Activity to Determine Mnemonic Processes Through which SPS Exposure Disrupts Extinction Retention. 6

7 2014 Keller, S., Schreiber, WB., Stanfield, BS, and Knox, D. Society for Neuroscience, Washington D.C. Paradoxical effects of glucocorticoids on extinction memory in the single prolonged stress model Schreiber, WB., Keller, S., and Knox, D. Society for Neuroscience, Washington D.C. The role of basal forebrain cholinergic neurons in cued extinction memory 2014 Keller, S., Schreiber, WB., Staib, J. and Knox, D. Pavlovian Society, Seattle WA. Sex differences in extinction retention deficits using an animal model of PTSD. Honors Served as third reader and thesis committee member for the Summer Scholars Program at the University of Delaware Invited by the American Psychological Association Directorate to publish a brief highlight of my research in the monthly Psychological Science Agenda Served as judge of research presentation competition for the Undergraduate Research Opportunity Program at the University of Michigan Honored at McNair/SROP annual conference at Michigan State University for excellence in academics Service and Professional Membership Former member of the Institutional Review Board for animal research at the Veterans Affairs Hospital in Ann Arbor MI (From 10/11 07/12) Former mentor in the Undergraduate Research Opportunity Program at the University of Michigan (From 10/11 07/12) Reviewer for Behavioural Brain Research, Biological Psychiatry, Emotion Review, European Journal of Neuroscience, eneuro, Frontiers in Behavioral Neuroscience, Journal of Neurochemistry, Learning & Memory, Molecular and Cellular Biochemistry, Neuropharmacology, Neuropsychopharmacology, Neuroscience, PLOS, Psychophysiology, Psychiatric Research, and Psychopharmacology. Member of the Society for Neuroscience and Pavlovian Society Neuroscience Techniques Behavioral Extensive experiencing in using MedPC software. Extensive experience designing and performing fear conditioning, extinction and recovery paradigms using fear conditioned freezing or fear conditioned operant suppression. 7

8 Extensive experience in using unconditioned defense behavioral paradigms (e.g. elevated plus maze, open field, predator odor generated defense behaviors) Can set up protocols in Anymaze (Stotelting Inc.) to automatically score freezing behavior Molecular Extensive experience in performing in situ hybridization in brain tissue Extensive experience in performing stereotaxic surgery for placement of probes in distinct brain regions (e.g. placing cannulas or electrodes in the prefrontal cortex) Extensive experience in protein isolation from brain tissue and quantification of isolated protein using the Western Blot technique Experience in using ELISA for protein, ACTH, and catecholamine quantification Extensive experience in performing immunocytochemistry in brain tissue qrt-pcr to assay RNA in brain tissue Electrophysiological Extensive experience in acquiring electrophysiological and psychophysiological recordings in behaving rats and analyzing these types of data Extensive experience in making glass electrodes for single unit electrophysiology, and analyze single unit electrophysiological data in Spike 2.0 8