St. Mary s College, Colwyn Bay, Clwyd, North Wales Rydal School, Colwyn Bay, Clwyd, North Wales. B.Sc. (Hons.) Biochemistry.

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1 CURRICULUM VITAE 1. PERSONAL DETAILS Name: Business Address: Shahram Salek-Ardakani, Ph.D. Department of Pathology, Immunology and Laboratory of Medicine University of Florida, College of Medicine 1275 Center Drive J583 BMSB [Biomedical Sciences Building] Gainesville, FL Phone (office): (352) Fax Number: (352) E mail: ssalek@ufl.edu 2. EDUCATION St. Mary s College, Colwyn Bay, Clwyd, North Wales. G.C.S.Es: Mathematics, Biology, Chemistry, Physics, Religious Studies, and English Rydal School, Colwyn Bay, Clwyd, North Wales. A Levels: Mathematics (pure and mechanics), Biology and Chemistry University of Kingston, U.K. B.Sc. (Hons.) Biochemistry. Thesis Title: Effect of Inhibitors of Polyamine Biosynthesis on the Growth of Leukemia Cells in Culture and an Investigation of Apoptosis as a Mechanism of Cell Death Paterson Institute for Cancer Research-University of Manchester. M.Sc. Immunology and Immunogenetics. Thesis Title: Cloning and Structural Analysis of Epstein Barr Virus-Encoded Gene, BCRF-1 (viral IL-10) Paterson Institute for Cancer Research-University of Manchester. Ph.D. Oncology/Viral Immunology. Mentors: Drs. Mike Mackett and John Arrand. Thesis Title: The Role of Epstein-Barr Virus-Encoded Interleukin- 10 During the EBV Life Cycle. 2. EMPLOYMENT HISTORY 1

2 2012-present Department of Pathology, Immunology and Laboratory of Medicine, University of Florida, College of Medicine Associate Professor Research areas: o T Cell Memory o TNFR/TNF Biology o Costimulation o Dendritic Cell Biology o Immunity to Respiratory Virus Infections o Mucosal Vaccines o Innate Immunity to Virus Infections o Viral Immune Modulatory Mechanisms La Jolla Institute for Allergy and Immunology, San Diego, California. Research Scientist/Instructor Research areas: T Cell Memory TNFR/TNF Biology Lung Allergic and Inflammatory Responses Immunity to Respiratory Virus Infections La Jolla Institute for Allergy and Immunology, San Diego, California. Post-Doctoral Fellow. Mentor: Dr. Michael Croft Research areas: Role of TNF receptor superfamily members, OX40, 4-1BB, LIGHT and HVEM in pulmonary lung inflammation driven by memory Th1/Tc1 and Th2/Tc2 cells. Role of PKC-theta in activation, differentiation and effector function of T cells using in vivo models of Th1/Tc1 and Th2/Tc2 lung inflammation and autoimmunity. Role of Bruton s tyrosine kinase in DC maturation and function using in vivo lung inflammation models. Role of E3 ubiquitin ligase, Itch, in CD4 T cell tolerance and lung inflammation. 4. AWARDS AND HONORS 2007 Arthritis National Research Foundation Travel Award ($1000) Center for Infectious Disease Young Investigator Award ($20000) Center for Infectious Disease Young Investigator Award ($40000) 2010 Keystone Symposia Future of Science Scholarship; Vaccine Mechanisms ($1200) Best Oral Presentation. 37 th Annual La Jolla Immunology conference 5. PROFESSIONAL MEMBERSHIPS 2009-present American Association of Immunologists 2

3 2009-present Cytokine and Inflammation Society 6. PATENTS United States Patent Methods of Treating OX40 Mediated Recall Immune Responses. This patent has been licensed by MedImmune, wholly owned subsidiary of AstraZeneca PLC, to explore its use in the development of a potential biologics drug for treating asthma. United States Patent LIGHT inhibitors for asthma, lung and airway inflammation, respiratory, interstitial, pulmonary and fibrotic disease treatment. United States Patent Virus Vaccination and treatment methods with OX40 agonist compositions. 7. EDITORIAL ACTIVITIES Editorial Board Member: Editor: Journal of Vaccines SOJ of Immunology Review Editor: Frontiers in Immunological memory Reviewer for: Journal of Immunology, Journal of Virology, European Journal of Immunology, PLoS One, PLoS Pathogens, Frontiers in Immunology, Human Immunology, Immunological Investigations, Vaccine, Cytokine, Clinical and Vaccine Immunology, Protein Expression and Purification, BMC Immunology, and Medical Science Monitor. Advisory Board Member: 2013 Advisory Board Member: 11th Cytokines & Inflammation Conference Advisory Board Member: 10th Cytokines & Inflammation Conference Advisory Board Member: 9th Cytokines & Inflammation Conference. NIH Study section 2014 Ad hoc reviewer: Vaccines Against Microbial Diseases study section (NIAID; June) Ad hoc reviewer: Vaccines Against Microbial Diseases study section (NIAID; February) Ad hoc reviewer: Vaccines Against Microbial Diseases study section (NIAID; December) Ad hoc reviewer: Vaccines Against Microbial Diseases study section (NIAID; June) Ad hoc reviewer: Vaccines Against Microbial Diseases study section (NIAID; February) Ad hoc reviewer: Special Emphasis Panel/Scientific Review Group 2013/01 ZAI1 MFH-I (J3) 1 (NIAID) Ad hoc reviewer: Vaccines Against Microbial Diseases study section (NIAID; February). Invited Talks th International TNF Conference th Cytokine and Inflammation Conference th Annual La Jolla Immunology Conference th Cytokine and Inflammation Conference 2010 Immunological Mechanisms of Vaccination (Keystone Symposia) 2010 American Association of Immunologists 97 th Annual Meeting th Cytokine and Inflammation Conference 3

4 2009 Harnessing Immunity to Treat and Prevent Disease (Cold Spring Harbor Laboratory) th La Jolla Immunology Conference 2008 Immune Mechanisms of Viral Control Annual Meeting (NIH/NIAID) 2007 Immune Mechanisms of Viral Control Annual Meeting (NIH/NIAID) 2007 Molecular & Immunological Approaches to Vaccine Design, (Cold Spring Harbor Laboratory) 2006 Frontiers of Clinical Investigation, (Host Defense 2006: From Bench to Bedside) 8. MENTORING EXPERIENCE Graduate Student Mentor 2013-Present Pretish Desai Postdoctoral Fellow Mentor 2012-present Georges Abboud 2011-present Vikas Talihiani John Goulding Junior Faculty Mentor 2012-present Tarun Hutchinson Graduate Student Committee Mentor 2012-present Robert Whitener (Ph.D. Student; Mentor, Clayton Mathews; Interdisciplinary Program in Biomedical Sciences, Department of Pathology, Immunology and Laboratory Medicine; University of Florida) Michael R Nelson (M.Sc. Student; Mentor, Shannon Wallet; Interdisciplinary Program in Biomedical Sciences, Department of Immunology & Microbiology, Periodontology; University of Florida). Undergraduate Student Mentor 2013-present Matthew Seskin 2013-present Kyle Varkoly Rebecka Bogue Graduate Classes (University of Florida, College of Medicine) 2014 GMS 6140 Principles of Immunology. Mucosal Immunology, T cell activation and differentiation, CTL effector function, APC regulation of the immune response, and T cell memory GM 6382 Advanced Cellular & Molecular Immunology. Mucosal Immunity GMS 6140 Principles of Immunology. Mucosal Immunology, T cell activation and differentiation, CTL effector function, APC regulation of the immune response, and T cell memory GMS 6140 Principles of Immunology. Mucosal Immunology. 9. GRANTS Completed R21 AI (S. Salek-Ardakani, PI) 09/30/07-08/31/10 NIH/NIAID Project Title: T Cell Memory to Respiratory Viral Infections 4

5 The aim of this application was to determine the role of OX40 in the generation of protective anti-viral CD8 T cell memory. Active R01 AI (S. Salek-Ardakani, PI) 12/01/10-12/31/15 NIH/NIAID; (Review group: Vaccines Against Microbial Diseases) Project Title: LIGHT-HVEM-BTLA Axis in Protective Anti-Viral Immunity The aim of this application is firstly to formulate a picture of the LIGHT-HVEM-BTLA interactions that may be required to generate a virus-reactive T cell population in various situations of alternate inflammation, and secondly to formulate a rationale strategy for targeting these molecules to promote long-term protective T cell memory against respiratory viral infections. 10. ORIGINAL PEER-REVIEWED PUBLICATIONS 1. Goulding J, Georges A, Tahiliani V, Desai P, Hutchinson T, Salek-Ardakani S. (2014) CD8 T cells Use IFN- to Protect against the Lethal Effects of Respiratory Poxvirus during Infection. J Immunol. (In Press) 2. Flynn R, Hutchinson T, Murphy KM, Ware CF, Croft M and Salek-Ardakani S*. (2013) CD8 T cell Memory to a Viral Pathogen Requires trans Cosignaling between HVEM and BTLA. PLos One. Oct 29;8(10):e Boettler T, Choi YS, Salek-Ardakani S, Cheng Y, Moeckel F, Croft M, Crotty S, Von Herrath M. Exogenous OX40 stimulation during lymphocytic choriomeningitis virus infection impairs follicular Th cell differentiation and diverts CD4 T cells into the effector lineage by upregulating Blimp-1. J Immunol. 191(10): Gerlic M, Faustin B, Postigo A, Yu EC, Proell M, Gombosuren N, Krajewska M, Flynn R, Croft M, Way M, Satterthwait A, Liddington RC, Salek-Ardakani S, Matsuzawa S, Reed JC. (2013) Vaccinia virus F1L protein promotes virulence by inhibiting inflammasome activation. Proc Natl Acad Sci U S A. 110(19): Zhao Y, Tahiliani V, Salek-Ardakani S, Croft M. (2012) Targeting 4-1BB (CD137) to enhance CD8 T cell responses with poxviruses and viral antigens. Frontiers in Immunology. Vol. 3 Article Boettler T, Moeckel F, Cheng Y, Heeg M, Salek-Ardakani S, Crotty S, Croft M, von Herrath MG. (2012) OX40 facilitates control of a persistent virus infection. PLoS Pathog. 8(9):e Goulding J, Bouge R, Tahiliani V, Croft M, Salek-Ardakani S*. (2012) CD8 T lymphocytes are essential for recovery from a respiratory vaccinia virus infection. J Immunol. 189(5): Salek-Ardakani S*, Moutaftsi M, Sette A, Croft M. (2011) Targeting OX40 Promotes Lung-Resident Memory CD8 T Cell Populations That Protect against Respiratory Poxvirus Infection. J Virol. 85(17): Salek-Ardakani S, Choi YS, Rafii-El-Idrissi Benhnia M, Flynn R, Arens R, Shoenberger S, Crotty S, Croft M, Salek-Ardakani S*. (2011) B cell-specific expression of B7-2 is required for follicular Th cell function in response to vaccinia virus. J Immunol. 186(9): Salek-Ardakani S*, Flynn R, Arens R, Yagita H, Smith GL, Borst J, Schoenberger SP, and Croft M. (2011) TNFR family members OX40 (CD134) and CD27 Link Viral Virulence to Protective T cell Vaccines. Journal of Clinical Investigation. 121(1):

6 11. Lee SW, Salek-Ardakani S, Mittler RS, and Croft M. (2009) Hyper-Costimulation through 4-1BB Distorts Homeostasis of Immune cells. J Immunol. 182(11): Zhao Y, De Trez C, Flynn R, Ware CF, Croft M, and Salek-Ardakani S*. (2009) The Adaptor Molecule MyD88 Directly Promotes CD8 T cell Responses to Vaccinia Virus. J Immunol. 182(10): Salek-Ardakani S*, Arens R, Flynn R, Sette A, Schoenberger SP, and Croft M. (2009) Preferential Use of B7.2 and Not B7.1 in Priming of Vaccinia Virus Specific CD8 T cells. J Immunol. 182 (5): Moutaftsi M, Salek-Ardakani S, Croft M, Bjoern P, Sidney J, Grey H, Sette A. (2009) Correlates of protection efficacy induced by VACV-specific CD8 T cell epitopes in the murine intranasal challenge model. Eur J Immunol. 39(3): Salek-Ardakani S*, Moutaftsi M, Crotty S, Sette A, and Croft M. (2008) OX40 Drives Protective Vaccinia Virus-Specific CD8 T cells. J Immunol. 181(11): Moutaftsi M, Hoa-Bui H, Peters B, Sidney J, Salek-Ardakani S, Oseroff C, Pasquetto V, Crotty S, Croft M, Grey A, and Sette A. (2007) VACV-specific CD4 + T cell responses target a set of antigens largely distinct from those targeted by CD8+ T cell responses. J Immunol. 178(11): Song J, Salek-Ardakani S, So T, Croft M. (2007) The kinases aurora B and mtor regulate the G1-S cell cycle progression of T lymphocytes. Nat Immunol. 8(1): Venuprasad K, Elly C, Gao M, Salek-Ardakani S, Harada Y, Luo JL,Yang C, Croft M, Inoue K, Karin M, Liu YC. (2006). Convergence of Itch-induced ubiquitination with MEKK1-JNK signaling in Th2 tolerance and airway inflammation. J Clin Invest. 116(4): Kawakami Y, Inagaki N, Salek-Ardakani S, Kitaura J, Tanaka H, Nagao K, Kawakami Y, Xiao W, Nagai H, Croft M, Kawakami T. (2006). Regulation of dendritic cell maturation and function by Bruton's tyrosine kinase via IL-10 and Stat3. PNAS. 103(1): Salek-Ardakani S, So T, Halteman BS, Altman A, Croft M. (2005). Protein kinase C- theta controls Th1 cells in experimental autoimmune encephalomyelitis. J Immunol. 175(11): Salek-Ardakani S, So T, Halteman BS, Altman A, and Croft M. (2004). Differential regulation of Th2 and Th1 lung inflammatory responses by protein kinase C theta. J Immunol. 173(10): Salek-Ardakani S*, Lyons SA, and Arrand JR. (2004). Epstein-Barr virus enhances human monocyte survival and maturation through a paracrine induction of IFN-. J Immunol. 173(1): So T, Salek-Ardakani S, Nakano H, Ware CF, and Croft M. (2004). TNF receptorassociated factor 5 limits the induction of Th2 immune responses. J Immunol. 172(7): Song J, Salek-Ardakani, S, Rogers PR, Cheng M, Van Parijs L, and Croft M. (2004). The costimulation-regulated duration of PKB activation controls T cell longevity. Nat Immunol. 5(2): Salek-Ardakani S, Halteman BS, Jember A, Akiba H, Yagita H, and Croft M. (2003). OX40 (CD134) controls memory T helper 2 cells that drive lung inflammation. J. Exp. Med. 198(2): Salek-Ardakani S*, Arrand JR, and Mackett M (2002). Epstein-Barr Virus Encoded Interleukin-10 Inhibits HLA-Class I, ICAM-1, and B7 Expression on Human Monocytes: Implications for Immune Evasion by EBV. Virology. 304(2):

7 27. Salek-Ardakani S*, Stuart AD, Arrand JE, Lyons SA, Arrand JR, and Mackett M. (2002). High level expression and purification of the Epstein-Barr virus encoded cytokine viral interleukin 10: efficient removal of endotoxin. Cytokine. 17(1): Salek-Ardakani S*, Arrand JR, Shaw D, and Mackett M. (2000). Heparin and heparan sulfate bind IL-10 and modulate its activity. Blood. 96(5): REVIEW ARTICLES 1. Salek-Ardakani S and Stephen P Schoenberger. (2013) T cell exhaustion: a means or an end? Nat Immunol. Jun;14(6): Goulding J, Tahiliani V, Salek-Ardakani S*. (2011) OX40:OX40L Axis: Emerging Targets for Improving Poxvirus-based CD8 T Cell Vaccines Against Respiratory Viruses. Immunol Rev. 244(1): Salek-Ardakani S* and Croft M. (2010) Tumor necrosis factor receptor/tumor necrosis factor family members in antiviral CD8 T-cell immunity. Journal of Interferon and Cytokine Research. 30(4): Salek-Ardakani S* and Croft M. (2009) T cells need NOD too? Nat Immunol. 10(12): Daniel Mucida and Shahram Salek-Ardakani*. (2009) Regulation of Th17 cells in the mucosal surfaces. J Allergy and Clin Immunol. 123(5): Salek-Ardakani S, Song A, Humphreys IA, and Croft M. (2006). OX40:OX40L Axis: Emerging Targets for Immunotherapy of Human Disease. Current Immunology Reviews. 2(1): Salek-Ardakani S*, Croft M. (2006). Regulation of CD4 T cell memory by OX40 (CD134). Vaccine. 24(7): BOOK CHAPTERS 1. Croft M, Salek-Ardakani S, Song J, So T, and Bansal-Pakala P. (2003). Regulation of T cell immunity by OX40 and OX40L. The TNF Superfamily. Landes Bioscience, Texas. 13. PRESENTATIONS 14 th International TNF Conference (Quebec City, Canada; July 7-10, 2013) CD8 T cell Memory to a Viral Pathogen Requires trans Cosignaling between HVEM and BTLA 11 th Cytokine and Inflammation Conference (San Diego, CA; January 29-31, 2013) CD8 T cell Derived IFN--Nipping Respiratory Viral Replication in the Epithelial Bud. 37 th Annual La Jolla Immunology Conference (San Diego, CA; October 11-13, 2011) HVEM/BTLA trans cosignaling regulates antiviral CD8 T memory. 9 th Cytokine and Inflammation Conference (San Diego, CA; January 27-28, 2011) 7

8 LIGHT-HVEM-BTLA Axis in Protective Anti-Viral CD8 T cell Immunity. Immunological Mechanisms of Vaccination (Keystone Symposia; Seattle, Washington, Oct-27-Nov1 2010) Immune Molecular Plasticity Links Viral Virulence to Protective T cell Vaccines. American Association of Immunologists 97 th Annual Meeting (Baltimore, MD; May 7-11, 2010) Immune Molecular Plasticity Links Viral Virulence to Protective T cell Vaccines. 8 th Cytokine and Inflammation Conference (San Diego, CA; January 28-29, 2010) TNF/TNFR Superfamily Members in Immunity to Respiratory Viral Infections. Harnessing Immunity to Treat and Prevent Disease (Cold Spring Harbor Laboratory; November 11-14, 2009) Viral Virulence Leads to Molecular Plasticity Required for Optimal Protective T cell Immunity. 35th La Jolla Immunology Conference (Salk Institute, La Jolla, CA; October 13 15, 2009) Viral Virulence Leads to Molecular Plasticity Required for Optimal Protective T cell Immunity. Immune Mechanisms of Viral Control Annual Meeting (NIH/NIAID, Washington; November 3-4, 2008) Virulence Mechanisms of Vaccinia Virus Determine the Selective Use of OX40 to Enhance Protective CD8 T cell Memory. Keystone Symposia on Molecular and Cellular Biology (Viral Immunity; Colorado, January 20-25, 2008). Molecular Plasticity for Protective Anti-Viral Immunity: Vaccinia Virus-Reactive CD8 T Cells Require OX40. Immune Mechanisms of Viral Control Annual Meeting (NIH/NIAID, Washington; November 25-27, 2007) T Cell Memory to Respiratory Viral Infections. Molecular & Immunological Approaches to Vaccine Design, (Cold Spring Harbor Laboratory; November 28-December 2, 2007) Molecular Plasticity for Protective Anti-Viral Immunity: Vaccinia Virus-Reactive CD8 T Cells Require OX40. Gordon Research Conference, Immunochemistry & Immunobiology, (March 25-30, 2007, Ventura, CA) Molecular plasticity for protective anti-viral immunity: Vaccinia virus-reactive CD8 T cells require OX40. Frontiers of Clinical Investigation, (Host Defense 2006: From Bench to Bedside; October 5-7, 2006, La Jolla, California) Protective CD8 T cell Responses Against Vaccinia Virus are Dependent on OX40 Signals. American Association of Immunologists 90 th Anniversary Meeting (Denver, Colorado May 6-10; 2003) OX40 (CD134) Costimulation Controls the Response of Memory T cells. Federation of American Societies for Experimental Biology (FASEB; New Orleans, 2002) 8

9 Critical role for OX40 (CD134) in Pulmonary Lung Inflammation Driven by Memory Th2 cells. "Abstracts," The FASEB Journal, April (2002). Joint Congress of the BSI and the BSACI (Harrogate; 1999) Immune Evasion by EBV. Possible Roles for Human and Viral IL-10. Interaction of IL-10 with Glycosaminoglycans. 2 nd Interleukin-10 workshop (Milan; 1999) Interaction of IL-10 with Glycosaminoglycans. 9