Journal of Microbes and Infection,June 2007,Vol 2,No. 2. (HBsAg)2 , (PCR) 1762/ 1764
|
|
|
- Blanche Fitzgerald
- 5 years ago
- Views:
Transcription
1 Journal of Microbes and Infection,June 2007,Vol 2,No. 2 2 S (HBsAg)2 ( YIC) S 5 30g 60g YIC ( HBV) DNA > 2 log10 e (HBeAg), 6 DNA, 1 YIC 1, (PCR) (0 ) (44 ) HBV DNA S 2, S a 2,YIC 3 HBV 1762/ 1764, 2 5 S ; ; 2 Studies on hepatitis B virus S gene mutation in chronic hepatitis B patients treated with HBsAg2HBIG immunogenic complex therapeutic vaccine MA Zhang2mei, TIAN Xiao2chen, LI Xiao2xiao, XU Dao2zhen, GUO Li2min, ZHAO Kai, WEN Yu2mei ( Keylaboratory of Medical Molecular Virology, Shanghai Medical College Fudan University, Shanghai ) Abstract Objective To study whether HBsAg2HBIG immunogenic complex therapeutic vaccine ( YIC) could induce S gene immune escape mutants1methods Five chronic hepatitis B patients who had been treated with 6 injections of either 30 or 60 micrograms of YIC were recruited for this study1 These patients were those who responded to YIC treatment, shown as a drop in serum HBV DNA virus load > 2 log 10, with serum HBeAg converted to negative at 24 weeks1 However, their virus load rebound at the end of 24 weeks follow2up1 As controls, one was a patient who did not respond to YIC treatment and remained serum HBV DNA positive at similar level during and after treatment, while the other one was a patient immunized with the alum placebo1 Serum samples before treatment and 6 months after termination of treatment were collected, and DNA extracted1 PCR was used to amplify HBV S gene, the precore/ core gene and the core promoter regions followed by sequencing1results In all 7 patients treated with YIC or alum no S gene mutation was found in theadeterminant, nor was precore stop codon mutation detected1 However, three patients had mutations in the 1762/ 1764 core2promoter region, other two patients had mutations in the core promoter region other than the 1762/ 1764 double mutation1conclusion The rebound of HBV replication in five YIC2treated patients was not due to the emergence of HBV S immune2escape mutants1 Key words Therapeutic vaccine ; Viral mutation ; Hepatitis B virus surface antigen2antibodies 315 ( HBsAg) HBsAg2 ( YIC) [124,50 %, ] 2002 (HBV),,, YIC HBV, HBV (2HBs) IFN2 22 ( IL2,, 2) [5 ] A 36, 60 g YIC 6,5/ 10, ( IFN) YIC HBV DNA > 2 log 10,, HBeAg 2HBe, IFN 2 HBsAg 1/ 3,IFN [6 ] B, 30 g 60g YIC YIC :11, ;21 ;31 :,E2mail edu. cn S,6 YIC 1 (0 ) (44 )
2 Journal of Microbes and Infection,June 2007,Vol 2,No HBV DNA S 2 (pre2c) C PCR ) 0 44 S YIC HBV DNA HBeAg 2 YIC B HBe 6 YIC 2 60g YIC ( # 52 # 92),4 30g YIC, YIC B, ( # 102 # 180 # 213 # 241) 1 ( # 150) g 60g YIC, g g YIC, 6 24 HBV DNA > 2 log 10 HBeAg, HBV DNA HBeAg 2HBe HBV DNA, 1 HBV DNA # 92 YIC # 52 1 # 102 # 180 # 213 # 241 YIC (0 ) 1 (44 ) 21 (1) DNA : 100 l 300 l TES ( 10mmol/ L, 2. YIC S a 015mmol/ L ph 810,015 %PCR 7 (0 ),200g/ ml K),65 3 (44 ) HBV DNA S h, / / ( ) g 10 min,215, # 150,1/ 10 3 mol/ L (ph 510) DNA, 70 % 1,DNA 20 (2) (PCR) : HBV l ) (2) # 150 S,C82 ( I) (T) S S5 : 5 - CTA GGA TCC ATG GAGAGC ACA ACA TCA G23( nt ), C82S3 : 5 2GCC GAA TTC TCA AAT GTA TAC CCA AAG AC23( nt ) C, Pc1 : 5 2CCC 3. YIC C PCR AAG GTC TTA CAT AAG AGG 23( nt ), Pc2 : 5 2GGT GGC CAA ATT CAT CAA CT23 HBV DNA C/ C ( nt ),7 C, (44 ) (3) PCR : S 681 bp, C ( ) YIC, 470 bp 50 l, 200 HBV C mol/l 4 dntp,1 PCR,10 pmol/ L C82S5 C82S3 Pc1 Pc2,Taq DNA 215U (C/ C,C,1 ) :94 3 min,94 (4) Vector NTI ( Invitrogen C/ C C, YIC HBV DNA > 2 log 10, HBeAg, HBV DNA, HBeAg (1), 6 S a ( , 126 [7 126 I/ T, a ] YIC, HBV S 7 (0 ) (44 ) 4. YIC C ( # 52) 44 T1762A/ A1764G, 30s,55 30s,72 1 min, ( # 180 # 213) A/ 1764G,44 10 minpcr 1 % 1762T/ 1764A# 92 # 241 C, (3)
3 Journal of Microbes and Infection,June 2007,Vol 2,No. 2 1 YIC HBV DNA HBeAg 2HBe Fig 1. Kinetics of serum HBV DNA, HBeAg, anti2hbe in seven YIC treated patients who showed rebound in viral load # 150 was injected with placebo, # 92 was non2responder, the others were responders during treatment, but virus rebound during follow2up1 (A) : Kinetics of serum HBeAg ; (B) : Kinetics of serum HBeAb ; (C) : Kinetics of HBV DNA1 2 YIC S a Fig 2. Sequence alignment of theadeterminant of S protein in seven YIC treated patients who showed rebound in viral load The amino acids in theadeterminant of S protein showed no changes in sample collected at 0 and 44 weeks (the shaded region shows with sequences different from the consensus sequences summarized in data from GenBank1 # 150 was immunized with placebo, and at 126 residue, amino acid I/ T does not change the antigenicity ofadeterminant) 1
4 Journal of Microbes and Infection,June 2007,Vol 2,No YIC C Fig 3. Sequence alignment of C promoter region in seven YIC treated patients who showed rebound in viral load Nucleotide mutations in the core promoter region of samples collected at 0 and 44 weeks from 7 patients (the shaded region shows sequences different from the consensus sequences summarized in data from GenBank) 1 2S22HBsAg [10 ] HBsAg2 2 ( YIC) S,, C/ C C HBV,, S,, [8 ], IFN, HBV DNA, ( / ),,NK, YIC, [9 ] HBV, 1 YIC HBeAg 1, 2HBe [6 ],, S, a HBsAg ( # 150) I,
5 Journal of Microbes and Infection,June 2007,Vol 2,No T,,T/ I T/ S T/ N, 31 Zheng BJ, Ng MH, He LF, et al1 Therapeutic efficacy of [7 a ], hepatitis B surface antigen2antibodies recombinant composite in HBsAg transgenic, mice1 Vaccine,2001,19 : Zheng BJ, Zhou J, Qu D, et al1 Selective functional deficit in dendritic cell2t cell interaction is a crucial mechanism in 60g YIC 30 g YIC chronic hepatitis B virus infection1 J Viral Hepat, 2004, 11 : ( ) YIC Xu DZ, Huang KL, Zhao K, et al1 Vaccination with PCR, recombinant HBsAg2HBIG complex in healthy adults1, HBV Vaccine,2005,23 : YIC 61 Yao X, Zheng BJ, Zhou J, et al1 Therapeutic effect of, S hepatitis B surface antigen2antibody complex is associated with HBsAg 20, cytolytic and non2cytolytic immune responses in hepatitis B patients1 Vaccine,2007,25 : S, YIC 71,,,1 HBV 1 C, 3 C 1762/ 1764, 2 C, C Resist Updat,2001,4 : C 1762/ 91 Kakimi K, Lane TE, Chisari FV, et al1 Inhibition of hepatitis 1764 virusreplication by activated NK T cells does not require [11 ], 5 1 inflammatory cell recuritment to the liver1 J Immunol, 2001, 5 C, 167 : Pol S, Nalpas B, Driss F, et al1 Efficacy and limitations of a specific immunotherapy in chronic hepatitis B1 J Hepatology, 11 Wen YM, Xiong SD, Zhang W1 Solid matrix antibody2antigen complex can clear viremia and antigenemia in persistent duck hepatitis B virus infection1 J Gen Virol,1994,75 : Wen YM, Qu D, Zhou SH, et al1 Antigen2antibody complex as therapeutic vaccine for viral hepatitis B1 Intern Rev Immunol,1999,18 : ( ), 2006,33 : Fischer KP, Gutfreund KS, Tyrrwll DL1 Lamivudine resistance in hepatitis B : Mechanisms and clinical implications1 Drug 2001,34 : ,,,1 C C S2 1,2006,24 : ( : ) ( 67 ) 3. Ma S, Boerner J E, Tiong Yip C, et al. NIM811, a cyclophilin inhibitor, exhibits potent in vitro activity against hepatitis c virus alone or in combination with alpha interferon. Antimicrob Agents Chemother,2006, 50 : Wieland SF, Chisari FV. Stealth and cunning : hepatitis B and hepatitis C viruses. J Virol,2005, 79 : Barber DL, Wherry EJ, Masopust D, et al. Restoring function in exhausted CD8 T cells during chronic viral infection. Nature, 2006, 439 : Biswal BK, Cherney MM, Wang M,et al. Crystal structures of the RNA2dependent RNA polymerase genotype 2a of hepatitis C virus reveal two conformations and suggested mechanisms of inhibition by non2nucleoside inhibitors. J Biol Chem 2005, 280 : ( : )
c Tuj1(-) apoptotic live 1 DIV 2 DIV 1 DIV 2 DIV Tuj1(+) Tuj1/GFP/DAPI Tuj1 DAPI GFP
Supplementary Figure 1 Establishment of the gain- and loss-of-function experiments and cell survival assays. a Relative expression of mature mir-484 30 20 10 0 **** **** NCP mir- 484P NCP mir- 484P b Relative
Supplementary Document
Supplementary Document 1. Supplementary Table legends 2. Supplementary Figure legends 3. Supplementary Tables 4. Supplementary Figures 5. Supplementary References 1. Supplementary Table legends Suppl.
Phylogenetic analysis of human and chicken importins. Only five of six importins were studied because
Supplementary Figure S1 Phylogenetic analysis of human and chicken importins. Only five of six importins were studied because importin-α6 was shown to be testis-specific. Human and chicken importin protein
Supplementary Table 3. 3 UTR primer sequences. Primer sequences used to amplify and clone the 3 UTR of each indicated gene are listed.
Supplemental Figure 1. DLKI-DIO3 mirna/mrna complementarity. Complementarity between the indicated DLK1-DIO3 cluster mirnas and the UTR of SOX2, SOX9, HIF1A, ZEB1, ZEB2, STAT3 and CDH1with mirsvr and PhastCons
Supplementary Appendix
Supplementary Appendix This appendix has been provided by the authors to give readers additional information about their work. Supplement to: Sherman SI, Wirth LJ, Droz J-P, et al. Motesanib diphosphate
Supplemental Data. Shin et al. Plant Cell. (2012) /tpc YFP N
MYC YFP N PIF5 YFP C N-TIC TIC Supplemental Data. Shin et al. Plant Cell. ()..5/tpc..95 Supplemental Figure. TIC interacts with MYC in the nucleus. Bimolecular fluorescence complementation assay using
Mutants and HBV vaccination. Dr. Ulus Salih Akarca Ege University, Izmir, Turkey
Mutants and HBV vaccination Dr. Ulus Salih Akarca Ege University, Izmir, Turkey Geographic Distribution of Chronic HBV Infection 400 million people are carrier of HBV Leading cause of cirrhosis and HCC
A Novel Recombinant Virus Reagent Products for Efficient Preparation Of Hepatitis B Animal Models
About FivePlus Beijing FivePlus Molecular Medicine Institute was established in 2005. The company has been dedicating itself to continuous innovation of viral vectors. The meaning of FivePlus is based
Diagnostic Methods of HBV and HDV infections
Diagnostic Methods of HBV and HDV infections Zohreh Sharifi,ph.D Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine Hepatitis B-laboratory diagnosis Detection
a) Primary cultures derived from the pancreas of an 11-week-old Pdx1-Cre; K-MADM-p53
1 2 3 4 5 6 7 8 9 10 Supplementary Figure 1. Induction of p53 LOH by MADM. a) Primary cultures derived from the pancreas of an 11-week-old Pdx1-Cre; K-MADM-p53 mouse revealed increased p53 KO/KO (green,
Basics of hepatitis B diagnostics. Dr Emma Page MRCP MD(Res) Locum Consultant Sexual Health & Virology
Basics of hepatitis B diagnostics Dr Emma Page MRCP MD(Res) Locum Consultant Sexual Health & Virology Basics of hepatitis B diagnostics Background Epidemiology Morphology Life-cycle Diagnostic markers
Characterizing intra-host influenza virus populations to predict emergence
Characterizing intra-host influenza virus populations to predict emergence June 12, 2012 Forum on Microbial Threats Washington, DC Elodie Ghedin Center for Vaccine Research Dept. Computational & Systems
HBsAg(+) mothers is a transient
Perinatal HBV viremia in newborns of HBsAg(+) mothers is a transient phenomenon that does not necessarily imply HBV infection transmission Vana Papaevangelou (Greece) National and Kapodistrian University
Therapeutic immunization strategies in chronic hepatitis B
Therapeutic immunization strategies in chronic hepatitis B Mengji Lu Institut für Virologie Universitätsklinkum Essen Falk Symposium Berlin, 04. October 2005 The aim of therapeutic immunization to achieve
Supplementary Figures
Supplementary Figures Supplementary Figure 1. H3F3B expression in lung cancer. a. Comparison of H3F3B expression in relapsed and non-relapsed lung cancer patients. b. Prognosis of two groups of lung cancer
A preliminary report on the influence of baseline cellular immunity to the therapeutic responses of peg-interferon
146 2009 9 4 3 Journal of Microbes and Infection, September 2009, Vol. 4, No. 3 e 2a 1, 2, 1, 1, 1, 1, 1, 1 1., 200025; 2., 200032 : ( CHB) ( IFN), IFN IFN, e ( HBeAg) CHB 19, 14, IFN- 2a 180 g, 1, 48,
Supplementary Table 2. Conserved regulatory elements in the promoters of CD36.
Supplementary Table 1. RT-qPCR primers for CD3, PPARg and CEBP. Assay Forward Primer Reverse Primer 1A CAT TTG TGG CCT TGT GCT CTT TGA TGA GTC ACA GAA AGA ATC AAT TC 1B AGG AAA TGA ACT GAT GAG TCA CAG
Nature Structural & Molecular Biology: doi: /nsmb Supplementary Figure 1
Supplementary Figure 1 U1 inhibition causes a shift of RNA-seq reads from exons to introns. (a) Evidence for the high purity of 4-shU-labeled RNAs used for RNA-seq. HeLa cells transfected with control
The Hepatitis B-e antigen-positive
The Hepatitis B-e antigen-positive Dental Student Developing an Equitable Policy Hepatitis B Virus Serology 1 HBsAg A protein on the surface of HBV; it can be detected in high levels in serum during acute
Supplementary Figure 1 MicroRNA expression in human synovial fibroblasts from different locations. MicroRNA, which were identified by RNAseq as most
Supplementary Figure 1 MicroRNA expression in human synovial fibroblasts from different locations. MicroRNA, which were identified by RNAseq as most differentially expressed between human synovial fibroblasts
Table S1. Oligonucleotides used for the in-house RT-PCR assays targeting the M, H7 or N9. Assay (s) Target Name Sequence (5 3 ) Comments
SUPPLEMENTAL INFORMATION 2 3 Table S. Oligonucleotides used for the in-house RT-PCR assays targeting the M, H7 or N9 genes. Assay (s) Target Name Sequence (5 3 ) Comments CDC M InfA Forward (NS), CDC M
Acute Hepatitis B Virus Infection with Recovery
Hepatitis B: Clear as Mud Melissa Osborn, MD, MSCR Assistant Professor Emory University School of Medicine Atlanta, GA 1 Objectives 1. Distinguish the various stages in the natural history of chronic hepatitis
Supplementary Figure 1. ROS induces rapid Sod1 nuclear localization in a dosagedependent manner. WT yeast cells (SZy1051) were treated with 4NQO at
Supplementary Figure 1. ROS induces rapid Sod1 nuclear localization in a dosagedependent manner. WT yeast cells (SZy1051) were treated with 4NQO at different concentrations for 30 min and analyzed for
Supplementary Figure 1 a
Supplementary Figure a Normalized expression/tbp (A.U.).6... Trip-br transcripts Trans Trans Trans b..5. Trip-br Ctrl LPS Normalized expression/tbp (A.U.) c Trip-br transcripts. adipocytes.... Trans Trans
HEPATITIS B: are escape mutants of concern?
VACCINATION: AN EVOLUTIONARY ENGINE FOR SPECIES? Fondation Mérieux Conference Centre Veyrier-du-Lac, France November 25-27, 2013 HEPATITIS B: are escape mutants of concern? Alessandro ZANETTI Department
BIOLOGY 621 Identification of the Snorks
Name: Date: Block: BIOLOGY 621 Identification of the Snorks INTRODUCTION: In this simulation activity, you will examine the DNA sequence of a fictitious organism - the Snork. Snorks were discovered on
Nucleotide Sequence of the Australian Bluetongue Virus Serotype 1 RNA Segment 10
J. gen. Virol. (1988), 69, 945-949. Printed in Great Britain 945 Key words: BTV/genome segment lo/nucleotide sequence Nucleotide Sequence of the Australian Bluetongue Virus Serotype 1 RNA Segment 10 By
Supplementary Materials
Supplementary Materials 1 Supplementary Table 1. List of primers used for quantitative PCR analysis. Gene name Gene symbol Accession IDs Sequence range Product Primer sequences size (bp) β-actin Actb gi
HBV. Next Generation Sequencing, data analysis and reporting. Presenter Leen-Jan van Doorn
HBV Next Generation Sequencing, data analysis and reporting Presenter Leen-Jan van Doorn HBV Forum 3 October 24 th, 2017 Marriott Marquis, Washington DC www.forumresearch.org HBV Biomarkers HBV biomarkers:
Supplementary Information. Bamboo shoot fiber prevents obesity in mice by. modulating the gut microbiota
Supplementary Information Bamboo shoot fiber prevents obesity in mice by modulating the gut microbiota Xiufen Li 1,2, Juan Guo 1, Kailong Ji 1,2, and Ping Zhang 1,* 1 Key Laboratory of Tropical Plant Resources
MedInform. HBV DNA loss in Bulgarian patients on NUC therapy. Speed related factors. (NUC related speed of HBV DNA loss in Bulgaria) Original Article
DOI: 10.18044/Medinform.201852.897 ISSUE 3, 2018 HBV DNA loss in Bulgarian patients on NUC therapy. Speed related factors. (NUC related speed of HBV DNA loss in Bulgaria) Donika Krasteva, Radosveta Tomova,
CD31 5'-AGA GAC GGT CTT GTC GCA GT-3' 5 ' -TAC TGG GCT TCG AGA GCA GT-3'
Table S1. The primer sets used for real-time RT-PCR analysis. Gene Forward Reverse VEGF PDGFB TGF-β MCP-1 5'-GTT GCA GCA TGA ATC TGA GG-3' 5'-GGA GAC TCT TCG AGG AGC ACT T-3' 5'-GAA TCA GGC ATC GAG AGA
Finding protein sites where resistance has evolved
Finding protein sites where resistance has evolved The amino acid (Ka) and synonymous (Ks) substitution rates Please sit in row K or forward The Berlin patient: first person cured of HIV Contracted HIV
Beta Thalassemia Case Study Introduction to Bioinformatics
Beta Thalassemia Case Study Sami Khuri Department of Computer Science San José State University San José, California, USA sami.khuri@sjsu.edu www.cs.sjsu.edu/faculty/khuri Outline v Hemoglobin v Alpha
Supplementary Figure 1
Metastatic melanoma Primary melanoma Healthy human skin Supplementary Figure 1 CD22 IgG4 Supplementary Figure 1: Immunohisochemical analysis of CD22+ (left) and IgG4 (right), cells (shown in red and indicated
Beta Thalassemia Sami Khuri Department of Computer Science San José State University Spring 2015
Bioinformatics in Medical Product Development SMPD 287 Three Beta Thalassemia Sami Khuri Department of Computer Science San José State University Hemoglobin Outline Anatomy of a gene Hemoglobinopathies
Citation for published version (APA): Oosterveer, M. H. (2009). Control of metabolic flux by nutrient sensors Groningen: s.n.
University of Groningen Control of metabolic flux by nutrient sensors Oosterveer, Maaike IMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish to cite from it.
Supplementary Figure 1
Supplementary Figure 1 Supplementary Figure 1: Cryopreservation alters CD62L expression by CD4 T cells. Freshly isolated (left) or cryopreserved PBMCs (right) were stained with the mix of antibodies described
Slides are the property of the author and AASLD. Permission is required from both AASLD and the author for reuse.
Inarigivir Demonstrates Potent Dose Dependent Anti-Viral Activity in HBV Treatment-Naïve Patients: Role of HBeAg Status and Baseline HBsAg in Anti-Viral Response MF Yuen, M. Elkhashab, CY Chen, YF Chen,
Figure S1. Analysis of genomic and cdna sequences of the targeted regions in WT-KI and
Figure S1. Analysis of genomic and sequences of the targeted regions in and indicated mutant KI cells, with WT and corresponding mutant sequences underlined. (A) cells; (B) K21E-KI cells; (C) D33A-KI cells;
29th Viral Hepatitis Prevention Board Meeting
29th Viral Hepatitis Prevention Board Meeting Madrid, November 2006 Treatment of chronic hepatitis B José M. Sánchez-Tapias Liver Unit Hospital Clínic University of Barcelona Spain CHRONIC HBV INFECTION
Supplementary materials: Predictors of response to pegylated interferon in chronic hepatitis B: a
Supplementary materials: Predictors of response to pegylated interferon in chronic hepatitis B: a real-world hospital-based analysis Yin-Chen Wang 1, Sien-Sing Yang 2*, Chien-Wei Su 1, Yuan-Jen Wang 3,
Mutation in the Precore Region of HBV in Chronic Hepatitis B Patients
Mutation in the Precore Region of HBV in Chronic Hepatitis B Patients Kader O. 1, Metwally D.E. 1, Helaly G. F. 1, ElBatouti G. A. 2, Hassan M B 3 and Elsawaf R. 1 1 Microbiology Department, Medical Research
ISSN X (Print) *Corresponding author Dr. Narotam Sharma
Scholars Journal of Applied Medical Sciences (SJAMS) Sch. J. App. Med. Sci., 2015; 3(9A):3134-3138 Scholars Academic and Scientific Publisher (An International Publisher for Academic and Scientific Resources)
Clinical Management of Hepatitis B WAN-CHENG CHOW DEPARTMENT OF GASTROENTEROLOGY & HEPATOLOGY SINGAPORE GENERAL HOSPITAL
Clinical Management of Hepatitis B WAN-CHENG CHOW DEPARTMENT OF GASTROENTEROLOGY & HEPATOLOGY SINGAPORE GENERAL HOSPITAL The World Health Organisation recent initiatives on HBV infection Launching of the
*To whom correspondence should be addressed. This PDF file includes:
www.sciencemag.org/cgi/content/full/science.1212182/dc1 Supporting Online Material for Partial Retraction to Detection of an Infectious Retrovirus, XMRV, in Blood Cells of Patients with Chronic Fatigue
Natural History of HBV Infection
Natural History of HBV Infection Joseph JY Sung MD PhD Institute of Digestive Disease Department of Medicine & Therapeutics Prince of Wales Hospital The Chinese University of Hong Kong HBV Infection 2
Toluidin-Staining of mast cells Ear tissue was fixed with Carnoy (60% ethanol, 30% chloroform, 10% acetic acid) overnight at 4 C, afterwards
Toluidin-Staining of mast cells Ear tissue was fixed with Carnoy (60% ethanol, 30% chloroform, 10% acetic acid) overnight at 4 C, afterwards incubated in 100 % ethanol overnight at 4 C and embedded in
A smart acid nanosystem for ultrasensitive. live cell mrna imaging by the target-triggered intracellular self-assembly
Electronic Supplementary Material (ESI) for Chemical Science. This journal is The Royal Society of Chemistry 2017 A smart ZnO@polydopamine-nucleic acid nanosystem for ultrasensitive live cell mrna imaging
Rama Nada. - Malik
- 2 - Rama Nada - - Malik 1 P a g e We talked about HAV in the previous lecture, now we ll continue the remaining types.. Hepatitis E It s similar to virus that infect swine, so its most likely infect
Hepatitis B Virus infection: virology
Hepatitis B Virus infection: virology 167 Falk Symposium: Liver under constant attack from fat to viruses III Falk Gastro-Konferenz 17.-21. September 2008 Congress Centrum Mainz Maura Dandri Department
Are booster immunisations needed for lifelong hepatitis B immunity?
Are booster immunisations needed for lifelong hepatitis B immunity? European Consensus Group on Hepatitis B immunity, following meeting in Florence in October 1998 To date there are no data to support
Abbreviations: P- paraffin-embedded section; C, cryosection; Bio-SA, biotin-streptavidin-conjugated fluorescein amplification.
Supplementary Table 1. Sequence of primers for real time PCR. Gene Forward primer Reverse primer S25 5 -GTG GTC CAC ACT ACT CTC TGA GTT TC-3 5 - GAC TTT CCG GCA TCC TTC TTC-3 Mafa cds 5 -CTT CAG CAA GGA
Diagnostic Methods of HBV infection. Zohreh Sharifi,ph.D of Virology Research center, Iranian Blood Transfusion Organization (IBTO)
Diagnostic Methods of HBV infection Zohreh Sharifi,ph.D of Virology Research center, Iranian Blood Transfusion Organization (IBTO) Hepatitis B-laboratory diagnosis Detection of HBV infection involves
Viral Hepatitis Diagnosis and Management
Viral Hepatitis Diagnosis and Management CLINICAL BACKGROUND Viral hepatitis is a relatively common disease (25 per 100,000 individuals in the United States) caused by a diverse group of hepatotropic agents
Nature Immunology: doi: /ni.3836
Supplementary Figure 1 Recombinant LIGHT-VTP induces pericyte contractility and endothelial cell activation. (a) Western blot showing purification steps for full length murine LIGHT-VTP (CGKRK) protein:
The Alphabet Soup of Viral Hepatitis Testing
The Alphabet Soup of Viral Hepatitis Testing August 18, 2011 Patricia Slev, PhD, DABCC Medical Director, Serologic Hepatitis and Retrovirus Laboratory, ARUP Laboratories Assistant Professor of Pathology,
Therapeutic strategies for immune reconstitution in acquired immunodeficiency syndrome
30 1, 1, 2, 3 1. ( ), 201508; 2., 200040; 3., 200032 : ( AIDS) ( HIV) 20 90,,,,,, AIDS, CD4 + T ( CTL), HIV, : ; ; Therapeutic strategies for immune reconstitution in acquired immunodeficiency syndrome
Supporting Information
Supporting Information Horwitz et al. 73/pnas.35295 A Copies ml - C 3NC7 7 697 698 7 7 73 76-2 2 Days Gp2 residue G458D G459D T278A 7/36 N28 K D 28 459 A28T ID# 697 ID# 698 ID# 7 ID# 7 ID# 73 ID# 76 ID#
Management of chronic hepatitis B : recent advance in the treatment of antiviral resistance
anagement of chronic hepatitis B : recent advance in the treatment of antiviral resistance / 김강모 연수강좌 anagement of chronic hepatitis B : recent advance in the treatment of antiviral resistance 김강모 울산대학교의과대학서울아산병원소화기내과
SUPPLEMENTARY DATA. Supplementary Table 1. Primer sequences for qrt-pcr
Supplementary Table 1. Primer sequences for qrt-pcr Gene PRDM16 UCP1 PGC1α Dio2 Elovl3 Cidea Cox8b PPARγ AP2 mttfam CyCs Nampt NRF1 16s-rRNA Hexokinase 2, intron 9 β-actin Primer Sequences 5'-CCA CCA GCG
Originally published as:
Originally published as: Ratsch, B.A., Bock, C.-T. Viral evolution in chronic hepatitis B: A branched way to HBeAg seroconversion and disease progression? (2013) Gut, 62 (9), pp. 1242-1243. DOI: 10.1136/gutjnl-2012-303681
ARTICLE IN PRESS. A Treatment Algorithm for the Management of Chronic Hepatitis B Virus Infection in the United States: An Update
CLINICAL GASTROENTEROLOGY AND HEPATOLOGY 2006;4:xxx REVIEW A Treatment Algorithm for the Management of Chronic Hepatitis B Virus Infection in the United States: An Update EMMET B. KEEFFE,* DOUGLAS T. DIETERICH,
Supplementary Figure 1
Supplementary Figure 1 3 3 3 1 1 Bregma -1.6mm 3 : Bregma Ref) Http://www.mbl.org/atlas165/atlas165_start.html Bregma -.18mm Supplementary Figure 1 Schematic representation of the utilized brain slice
University of Groningen. Vasoregression in incipient diabetic retinopathy Pfister, Frederick
University of Groningen Vasoregression in incipient diabetic retinopathy Pfister, Frederick IMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish to cite from
HBV Core and Core-Related Antigen Quantitation in Chinese Patients with. Chronic Hepatitis B Genotype B and C Virus Infection
Title page HBV Core and Core-Related Antigen Quantitation in Chinese Patients with Chronic Hepatitis B Genotype B and C Virus Infection Short Title: Quantitation of HBc and HBcrAg in Chinese patients Akinori
Dynamics of HBV DNA Levels, HBV Mutations and Biochemical Parameters during Antiviral Therapy in a Patient with HBeAg-Negative Chronic Hepatitis B
ASIAN PACIFIC JOURNAL OF ALLERGY AND IMMUNOLOGY (2007) 25: 183-188 CASE REPORT Dynamics of HBV DNA Levels, HBV Mutations and Biochemical Parameters during Antiviral Therapy in a Patient with HBeAg-Negative
To test the possible source of the HBV infection outside the study family, we searched the Genbank
Supplementary Discussion The source of hepatitis B virus infection To test the possible source of the HBV infection outside the study family, we searched the Genbank and HBV Database (http://hbvdb.ibcp.fr),
casebasedhepatitis bmanagement Management of Hepatitis B: A Case-based Approach
casebasedhepatitis bmanagement Management of Hepatitis B: A Case-based Approach In order to receive credit for this activity, please complete the post-test by recording the best answer to each question
Influenza viruses are classified as members of the family
Rewiring the RNAs of influenza virus to prevent reassortment Qinshan Gao a and Peter Palese a,b,1 Departments of a Microbiology and b Medicine, Mount Sinai School of Medicine, New York, NY 10029 Contributed
Mutation Screening and Association Studies of the Human UCP 3 Gene in Normoglycemic and NIDDM Morbidly Obese Patients
Mutation Screening and Association Studies of the Human UCP 3 Gene in Normoglycemic and NIDDM Morbidly Obese Patients Shuichi OTABE, Karine CLEMENT, Séverine DUBOIS, Frederic LEPRETRE, Veronique PELLOUX,
www.lessonplansinc.com Topic: Protein Synthesis - Sentence Activity Summary: Students will simulate transcription and translation by building a sentence/polypeptide from words/amino acids. Goals & Objectives:
Corresponding author: M.H. Lu
Changes in peripheral blood natural killer T cells in hepatitis B e antigen-positive chronic hepatitis B patients and efficacy prediction after pegylated interferon therapy F. Huang 1,2, M.H. Lu 1, H.Y.
Culture Density (OD600) 0.1. Culture Density (OD600) Culture Density (OD600) Culture Density (OD600) Culture Density (OD600)
A. B. C. D. E. PA JSRI JSRI 2 PA DSAM DSAM 2 DSAM 3 PA LNAP LNAP 2 LNAP 3 PAO Fcor Fcor 2 Fcor 3 PAO Wtho Wtho 2 Wtho 3 Wtho 4 DTSB Low Iron 2 4 6 8 2 4 6 8 2 22 DTSB Low Iron 2 4 6 8 2 4 6 8 2 22 DTSB
Patterns of hemagglutinin evolution and the epidemiology of influenza
2 8 US Annual Mortality Rate All causes Infectious Disease Patterns of hemagglutinin evolution and the epidemiology of influenza DIMACS Working Group on Genetics and Evolution of Pathogens, 25 Nov 3 Deaths
HBeAg-positve chronic hepatts B: Why do I treat my patent with a NA? Maria But
HBeAg-positve chronic hepatts B: Why do I treat my patent with a NA? Maria But Hospital Universitario Valle Hebron and Ciberehd del Insttuto Carlos III. Barcelona. Spain Disclosures Advisory board of,
Cornerstones of Hepatitis B: Past, Present and Future
Cornerstones of Hepatitis B: Past, Present and Future Professor Man-Fung Yuen Queen Mary Hospital The University of Hong Kong Hong Kong 1 Outline Past Natural history studies Development of HBV-related
Section Lectures: Immunology/Virology Time: 9:00 am 10:00 am LRC 105 A & B
Section Director: Cliff Bellone, Ph.D. Office: Doisy Hall - R 405 Phone: 577-8449 E-Mail: bellonec@slu.edu Lecturers: James Swierkosz, Ph.D. Office: Medical School Rm. 412 Phone: 577-8430 E-Mail: swierkoszje@slu.edu
[DOI] /j.issn , China
![[DOI] /j.issn , China](/thumbs/93/111250296.jpg "[DOI] /j.issn , China")
Med J Chin PLA, Vol. 41, No. 5, May 1, 2016 351 HBsAg HBs S N- [ ] (HBV)HBsAg+ HBs S (MHR) N- HBsAg+ HBs 284 HBsAg+ HBs 314 HBsAg HBV S 1 MHR N- N- S/S HepG2 HBsAg+ HBs MHR N- 11.3%(32/284) HBsAg 2.9%(9/314)(P
Hepatitis B virus infection. Chow Wan Cheng Dept of Gastroenterology & Hepatology Singapore General Hospital
Hepatitis B virus infection Chow Wan Cheng Dept of Gastroenterology & Hepatology Singapore General Hospital Chronic Hepatitis B - are we in the same situation as hepatitis C? Treatment of chronic hepatitis
HIV . HIV-1 HIV. . NJplot. Nested RT-PCR . CRF35-AD
(-) HIV-1 POL HIV.. HIV-1 HIV Nested RT- HIV-1. PTZ-57RT PCR.. ClustalW /. NJplot Nested RT-PCR.. CRF35-AD. CRF35-AD. HIV1 : // : // : : PhD : - PhD - HIV-1 POL. HIV.. HIV-1 PCR CRFs= ) (Criculating Recombinant
Virion Genome Genes and proteins Viruses and hosts Diseases Distinctive characteristics
Hepadnaviruses Virion Genome Genes and proteins Viruses and hosts Diseases Distinctive characteristics Hepatitis viruses A group of unrelated pathogens termed hepatitis viruses cause the vast majority
Choice of Oral Drug for Hepatitis B: Status Asokananda Konar
Choice of Oral Drug for Hepatitis B: Status 2011 Asokananda Konar Chronic hepatitis B (CHB) is a global public health challenge with an estimated 350 to 400 million people with chronic HBV infection, despite
Hepatitis B virus core-related antigen is a serum prediction marker for hepatocellular carcinoma
Editorial Hepatitis B virus core-related antigen is a serum prediction marker for hepatocellular carcinoma Kazunori Kawaguchi, Masao Honda, Shuichi Kaneko Department of Gastroenterology, Kanazawa University
Relationship of the APOA5/A4/C3/A1 gene cluster and APOB gene polymorphisms with dyslipidemia
elationship of the APOA5/A4/C3/A1 gene cluster and APOB gene polymorphisms with dyslipidemia H.J. Ou 1, G. Huang 2, W. Liu 3, X.L. Ma 2, Y. Wei 4, T. Zhou 5 and Z.M. Pan 3 1 Department of Neurology, The
Supplementary Figure 1
Supplementary Figure 1 Supplementary Figure 1. Lats1/2 deleted ihbs and ihps showed decreased transcripts of hepatocyte related genes (a and b) Western blots (a) and recombination PCR (b) of control and
Hepatitis B Update. Jorge L. Herrera, M.D. University of South Alabama Mobile, AL. Gastroenterology
Hepatitis B Update Jorge L. Herrera, M.D. University of South Alabama Mobile, AL Deciding Who to Treat Is hepatitis B a viral disease or a liver disease? Importance of HBV-DNA Levels in the Natural History
Optimized HBV Treatment Through Baseline and on-treatment Predictor Oral Antiviral Therapy. Watcharasak Chotiyaputta
Optimized HBV Treatment Through Baseline and on-treatment Predictor Oral Antiviral Therapy Watcharasak Chotiyaputta Progression of Liver Disease Goal of HBV Treatment: prevention the development of cirrhosis
ISBT TTI WP HBV safety subgroup. Review and update
ISBT TTI WP HBV safety subgroup Review and update Distribution of dominant HBV genotypes countries & centres participating in HBV studies A2/A1 0.1-0.5 A2 D 0.1-8 B/C F 1-5 E 12-25 5-12 A1 4-8 A2/C HBV
Zheng, BJ; Du, LY; Zhao, GY; Lin, YP; Sui, HY; Chan, C; Ma, S; Guan, Y; Yuen, KY. Citation Hong Kong Medical Journal, 2008, v. 14 suppl. 4, p.
Title Studies of SARS virus vaccines Author(s) Zheng, BJ; Du, LY; Zhao, GY; Lin, YP; Sui, HY; Chan, C; Ma, S; Guan, Y; Yuen, KY Citation Hong Kong Medical Journal, 2008, v. 14 suppl. 4, p. 39-43 Issued
Modeling Kinetics of HBV Infection and Recommendations for Moving Forward
Modeling Kinetics of HBV Infection and Recommendations for Moving Forward Alan S. Perelson Theoretical Biology and Biophysics Los Alamos National Laboratory Los Alamos, NM HBV Models Hepatology 1999 Tsiang
Durability Of Lamivudine Associated HBe Antigen Seroconversion in Chinese-Canadian Patients with Chronic Hepatitis B Virus Infection
ISPUB.COM The Internet Journal of Gastroenterology Volume 4 Number 2 Durability Of Lamivudine Associated HBe Antigen Seroconversion in Chinese-Canadian Patients with Chronic Hepatitis B Virus Infection
Identification of hepatitis B virus DNA reverse transcriptase variants associated with partial response to entecavir
Title Identification of hepatitis B virus DNA reverse transcriptase variants associated with partial response to entecavir Author(s) Wong, DKH; Fung, JYY; Lai, CL; Yuen, RMF Citation Hong Kong Medical
Whats new on HBsAg and other markers for HBV infection? Christoph Höner zu Siederdissen
Whats new on HBsAg and other markers for HBV infection? Christoph Höner zu Siederdissen Why diagnostic markers are important They are the basis for clinical decision makings treatment or no treatment?
Viral hepatitis. The word hepatitis means inflammation of the liver. There are five main types of viral hepatitis: A, B, C, D, E
Viral hepatitis The word hepatitis means inflammation of the liver There are five main types of viral hepatitis: A, B, C, D, E Hepatitis A and E are typically caused by ingestion of contaminated food or
Pre-Assessment Review: Microbiology, Part 2: Virology. Dr. David Hillyard
Pre-Assessment Review: Microbiology, Part 2: Virology Dr. David Hillyard 1. Mutations or deletions within which gene are associated with the hyper-toxin producing strain of Clostridium difficile? a. tcda
Yuen, MF; Sablon, E; Yuan, HJ; Hui, CK; Wong, DKH; Doutreloigne, J; Wong, BCY; Chan, AOO; Lai, CL
Title Author(s) Relationship between the development of precore and core promoter mutations and hepatitis B e antigen seroconversion in patients with chronic hepatitis B virus Yuen, MF; Sablon, E; Yuan,
Sequence analysis for VP4 of enterovirus 71 isolated in Beijing during 2007 to 2008
16 2009 3 4 1 Journal of Microbes and Infection, March 2009, Vol. 4, No. 1 2007 2008 71 VP4 1, 2, 2, 2, 1, 2, 2, 2, 1, 2 1., 100730; 2., 100020 : 2007 2008 71 ( EV71), 2007 3 EV71( 1, 2 ) 2008 5 EV71(