Body Fluid Identification Test No Summary Report

Transcription

1 Collaborative Testing Services, Inc FORENSIC TESTING PROGRAM Body Fluid Identification Test No Summary Report This proficiency test was sent to 219 participants. Each participant received a sample pack containing six items for examination. Participants were asked to examine each item and report the body fluid(s) identified. Data were returned from 151 participants (69% response rate) and are compiled into the following tables: Manufacturer's Information Summary Comments Key to Screening Table 1: Screening Conclusions & Test(s) Performed Table 2: Serology Screening Table 3: Additional Comments Appendix: Data Sheet Page This report contains the data received from the participants in this test. Since these participants are located in many countries around the world, and it is their option how the samples are to be used (e.g., training exercise, known or blind proficiency testing, research and development of new techniques, etc.), the results compiled in the Summary Report are not intended to be an overview of the quality of work performed in the profession and cannot be interpreted as such. The Summary Comments are included for the benefit of participants to assist with maintaining or enhancing the quality of their results. These comments are not intended to reflect the general state of the art within the profession. Participant results are reported using a randomly assigned "WebCode". This code maintains participant's anonymity, provides linking of the various report sections, and will change with every report.

2 Manufacturer's Information Each sample pack consisted of six items for testing, four of which were swabs and two of which were evidence items. Participants were asked to examine Items 1 and 2 for the body fluids that they typically encounter in casework. Items 3-6 were to be examined for scenario specific body fluids. SAMPLE PREPARATION: The stains on Item 1 (white swabs) and Item 4 (yellow swabs) were prepared using human semen from a single donor. The stain on Item 2 (pink swabs) was prepared using human saliva. The stains on Item 3 (blue swabs) and Item 5 (black headpiece) were prepared using human whole blood which was drawn into citric acid preservative bloodbank bags. The stain on Item 6 (red women's underwear) was a mixture prepared from human whole blood and semen. The raw semen was from a single donor, stored frozen at a temperature below -20 C. The semen was thawed and mixed 1:1 with TAE buffer, and then mixed 1:1 with the blood. This mixture was used to spot the Item 6 substrate. Stains from different sources were prepared at separate times and were packaged once they were thoroughly dried. SAMPLE SET ASSEMBLY: Each sample set contained a package of unused swabs, questioned swabs (Items 1, 2, 3 and 4) and two questioned items of evidence, a black headpiece (Item 5) and a pair of red women's underwear (Item 6) which were placed in a pre-labeled sample pack envelope. The sealed sample pack envelopes were then packaged in pre-labeled Heat Seal envelopes and sealed. This process was repeated until all of the sample sets were prepared. VERIFICATION: Laboratories that conducted predistribution analysis of the samples reported consistent body fluid conclusions. Sample Preparation Schedule Item Substrate Fluid Volume 1 4 White swabs Semen 100 μl/swab 2 4 Pink swabs Saliva 100 μl/swab 3 4 Blue swabs Blood 100 μl/swab 4 4 Yellow swabs Semen 100 μl/swab 5 Black Du-rag Blood 100 μl 6 Red women's underwear Blood and Semen 100 μl Completed sample sets were stored at -20 C until shipment on September 09, 2014, following receipt of predistribution results. Release Date of Manufacturer's Information: 01-December-2014

3 Summary Comments The Body Fluid Identification test was designed to allow participants to assess their proficiency in the identification of stains that may contain dried body fluids. Each participant received six items for testing, four of which were swabs and two were items of clothing. No scenario was provided for Items 1 and 2; participants were requested to test for body fluids that they typically encounter in casework. Scenarios were provided for the remaining items; participants were asked to treat these as they would casework. Item 1 consisted of four swabs containing semen. For blood, the majority (91%) of participants reporting a conclusion were negative, 10 were positive/indicative and one was inconclusive. For semen, the majority (98%) of participants reporting a conclusion for semen were positive/indicative and three were negative. For saliva, a consensus conclusion could not be established, there were 85 participants that were negative, 20 that were positive/indicative and 10 that were inconclusive. Item 2 consisted of four swabs containing saliva. For blood, the majority (98%) of participants reporting a conclusion were negative, one was positive/indicative and one was inconclusive. For semen, the majority (99%) of participants reporting a conclusion were negative and one was positive/indicative. For saliva, all participants reporting a conclusion were positive/indicative. Item 3 consisted of four swabs containing blood. For blood, the majority (98%) of participants reporting a conclusion were positive/indicative and three were negative. For semen, all participants reporting a conclusion were negative. For saliva, the majority (92.6%) of participants reporting a conclusion were negative, five were inconclusive and one was positive/indicative. Item 4 consisted of four swabs containing semen. For blood, the majority (94%) of participants reporting a conclusion were negative, five were positive/indicative and one was inconclusive. For semen, the majority (98%) of participants reporting a conclusion were positive/indicative and three were negative. For saliva, the majority (80%) of participants reporting a conclusion were negative, 11 were positive/indicative and nine were inconclusive. Item 5 consisted of one black headpiece containing blood. For blood, all participants reporting a conclusion were positive/indicative. For semen, the majority (97%) of participants reporting a conclusion were negative, two were positive/indicative and one was inconclusive. For saliva, the majority (97%) of participants reporting a conclusion were negative and two were inconclusive. Item 6 consisted of one red women's underwear containing a mixture of blood and semen. For blood, all participants reporting a conclusion were positive/indicative. For semen, the majority (97%) of participants reporting a conclusion were positive/indicative, three were negative and one was inconclusive. For saliva, the majority (95%) of participants reporting a conclusion were negative, two were positive/indicative and two were inconclusive. Release Date of Summary Report: 13-January-2015

4 Key for Screening Tests Used Participants were asked to use, where possible, the following chart of abreviated screening test names. This was not an all inclusive list and was not designed to determine what tests should be performed. Participants were advised that tests not on this list may be used for screening. Test Acid Phosphatase Kastle Meyer Leucomalachite Green scopic Ortho-tolidine Phenolphthalein-Tetramethyl benzidin Prostate Specific Antigen Tetramethyl benzidine Abbreviation PTMB TMB

5 Screening Conclusions & Test(s) Performed Indicate the overall conclusion from the results of the screening tests performed. Table 1 - Item 1 27CG2C Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed MICRO PHADEBAS 28BLGK / Indicative Not Tested 2EMTZU / Indicative / Indicative 2FHERZ / Indicative micro Saliva 2PQVGK / Indicative Other Tests Performed Sperm Search 2VEVGY / Indicative / Indicative 3FF4BN / Indicative 3MXG82 / Indicative Inconclusive tube test Other Tests Performed Urine - Creatinine Urine - DMAC Faeces - Edelmanns 3P4YCN / Indicative -Saliva Test No ( 5 ) Copyright 2015 CTS, Inc

6 Table 1 - Item 1 Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed 4ZYB / Indicative Amylase (radial diffusion) 4BJLQG / Indicative Not Tested 4HXUT4 / Indicative Not Tested 4JVXRK / Indicative / Indicative 68YCXV / Indicative OT 6HVCDK / Indicative Visual -Saliva micro 6PZ4XB / Indicative / Indicative 6UWLGW / Indicative Inconclusive 6ZGMFL Not Tested / Indicative Not Tested Swab was not red stained 7M6VAR Not Tested / Indicative No visible stain observed., Amylase 7MNLCK Not Tested / Indicative Not Tested Swabs have no red stains scopic Per Agency's Policy Test No ( 6 ) Copyright 2015 CTS, Inc

7 Table 1 - Item 1 Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed 7XNELB / Indicative KPIC 8C6JGB / Indicative Other Tests Performed Urine - Creatinine Feces - Urobilinogen 8NUMDA / Indicative / Indicative Not Tested This laboratory does not perform testing for saliva. 8PP7HW Not Tested Inconclusive test tube test 8XLW8D / Indicative Visual - Saliva 8YD3JW Not Tested / Indicative Yellowish staining observed, but no visible blood-like staining observed, therefore no presumptive test for blood performed : x4 positive : 2+ heads & tails, negative for NECs : x4 negative 9B677N / Indicative / Indicative 9GQWTH / Indicative 9HLHJN / Indicative / Indicative Test No ( 7 ) Copyright 2015 CTS, Inc

8 Table 1 - Item 1 Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed 9JH363 / Indicative Press 9Q3XCX / Indicative / Indicative AMYLASE DIFFUSION MICRO 9UE4NY Not Tested / Indicative Visual exam - no staining observed Amylase Diffusion 9W3CXZ / Indicative Press Semiquant AWHZRY / Indicative B2DAF7 / Indicative / Indicative Hemastix - wk pos 25 secs Hematrace - pos 3 mins - pos 8 secs - pos p30 - pos 1 min - neg 8.5 min B33FWW Not Tested / Indicative Not Tested Not tested: No red-brown staining observed on the Items. BA8DNA / Indicative Visual Spermatozoa () BHXXRM Not Tested / Indicative Not tested due to lack of reddish-brown color on swabs. Item 1 AnB:, Item 1 CnD:, Item 1 AnB: Item 1 CnD: BMUKKB / Indicative Seratec Test No ( 8 ) Copyright 2015 CTS, Inc

9 Table 1 - Item 1 Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed BNKQXU / Indicative / Indicative Hematrace BU8H89 / Indicative Not Tested BYM4XV Not Tested / Indicative no apparent bloodstain Amylase diffusion observed C4UYB4 / Indicative / Indicative Not Tested Saliva testing is not offered as a service in this laboratory. CGLJZ8 / Indicative Amylase Other Tests Performed Urine - Creatinine Urine - DMAC Fecal material - Urobilinogen DL73AZ / Indicative Press EB38QV / Indicative PHADEBAS MICRO EB9GR9 / Indicative TMB Saliva Field Kit EFF3F6 / Indicative (Seri ) Amylase () (Xmas Stain) Other Tests Performed Urine - Creatinine, DMAC Fecal Material - Urobilinogen Test No ( 9 ) Copyright 2015 CTS, Inc

10 Table 1 - Item 1 Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed ERDV3B / Indicative EVPZDB Not Tested / Indicative Not Tested White scene or kit swabs not Saliva testing not currently tested for blood in this lab being done in this lab EXGQ74 / Indicative (S) Amylase (ABA) (Xmas) Other Tests Performed Urine - Creatinine Fecal Material - Urobilinogen F42C9M Phenolphthalein SaligaE FVMNEL / Indicative O-Tol FXB6NT / Indicative G74RZB / Indicative / Indicative G8XFEZ / Indicative / Indicative Not Tested TMB Haemochromogen scopic Other Tests Performed Ouchterlony (Human origin) G9NLVQ / Indicative PHADEBAS PER TEST GFC6FU / Indicative Test No ( 10 ) Copyright 2015 CTS, Inc

11 Table 1 - Item 1 Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed GPVJEP / Indicative paper Visual exam H&E Slide GV43GN Not Tested / Indicative no staining on swab Amylase diffusion GZXZM8 Not Tested / Indicative Not Tested *swabs were not red stained H7J77Z / Indicative (Map) a-amylase (Xmas) Other Tests Performed Urine - Creatinine Fecal Material - Urobilinogen H9ADNB / Indicative / Indicative HDYUYF / Indicative / Indicative Not Tested Abacard Hematrace Abacard p30 HEDJ2T / Indicative ABAcard ABAcard test HHBZEM Not Tested / Indicative A red stain was not observed. Amylase diffusion HQ63HJ / Indicative HWRXDW / Indicative Radial Diffusion Test No ( 11 ) Copyright 2015 CTS, Inc

12 Table 1 - Item 1 Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed J23ATA / Indicative Not Tested -Semen Saliva test is not done if item is tested positive for blood or semen. No saliva test was done for Item 1 as it was tested positive for semen J94HFP Not Tested / Indicative JHVYAZ / Indicative Other Tests Performed Urine - Creatinine Feces - Urobilinogen JJM2UU / Indicative Inconclusive tube test Other Tests Performed Urine - DMAC JNFWZ9 / Indicative K-M -Saliva Sperm search w/ microscope Other Tests Performed Urine - Visual & DMAC Feces - Visual & scopy JPQ3UN / Indicative PHADEBAS PER TEST JRWLWQ / Indicative Not Tested Visual Exam Fast blue (+ve) () Current protocol does not allow (+ve) for Saliva testing on swabs. JTMRDG / Indicative -Semen scopy -Saliva Test No ( 12 ) Copyright 2015 CTS, Inc

13 Table 1 - Item 1 Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed JYWP6A / Indicative Not Tested Saliva test is not done if item is tested positive for either blood or semen. Item 1 is tested positive for semen, thus no saliva test was performed. K6XJEV / Indicative / Indicative K7UYQR / Indicative / Indicative Visual KQ3XGF / Indicative Inconclusive ABAcard p30 radial diffusion KUE3RF / Indicative O-TOL MICRO KXYWNG / Indicative ABAcard Saligae KZ3R7Y / Indicative Not Tested scopic LXURT6 / Indicative Visual exam M9CTN6 / Indicative Visual MED8A7 / Indicative O-Tol Test No ( 13 ) Copyright 2015 CTS, Inc

14 Table 1 - Item 1 Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed MEWRED Not Tested / Indicative Swabs were not tested for blood - does not display the characteristic reddish-brown stain associated with blood.,,,, Saliva Saliva Saliva Saliva MQAX88 / Indicative Not Tested MY39JM / Indicative Not Tested *saliva testing not performed in this lab MZHQHM / Indicative Not Tested Hemastix Fast Blue () Current protocol does not allow for Saliva testing on swabs. N3ZCLU / Indicative Inconclusive p30 KPIC N9PM3J Not Tested / Indicative No red-brown discoloration Amylase Diffusion N9PRB2 / Indicative (sperm search) NQ6YNH Not Tested / Indicative -Saliva P8P4F9 Not Tested / Indicative No reddish/brown stain observed during visual inspection. scopy Saliva PCGF7Y / Indicative Amylase testing by Radial Diffusion Test No ( 14 ) Copyright 2015 CTS, Inc

15 Table 1 - Item 1 Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed PDCWKG / Indicative ABA Card SALIgAE PJ2EYR / Indicative / Indicative Not Tested Hematrace Testing not offered. PJ7RQU / Indicative Seratec PKWW3E / Indicative Inconclusive Visual Slide made () PMLJF3 Not Tested / Indicative / Indicative *NT because no red brown stain observed. PNF7UR Inconclusive / Indicative TMB (amylase) Other Tests Performed Human - Ouchterlony Faeces - Price (urobilin) QADXPH / Indicative Hemastix (TMB) scopy a-amylase QP6VRD / Indicative / Indicative BCIP Amylase Diffusion scopic Sperm Search R9VBPE / Indicative Not Tested scopy Test No ( 15 ) Copyright 2015 CTS, Inc

16 Table 1 - Item 1 Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed RBKG8Q / Indicative P-30 KPIC RDTPGB / Indicative RNACNQ / Indicative / Indicative TMB Amylase Haemachromogen[sic] Other Tests Performed Faeces - Prices RNQTHJ / Indicative Not Tested Visually RVQ877 Not Tested / Indicative No visual apparent blood staining Amylase diffusion T2V4RR / Indicative Seratec T7LPCY / Indicative Not Tested scopic No currently validated procedures for saliva test. TJBFHW / Indicative / Indicative TKLJQE / Indicative Amylase Diffusion TX4EYX / Indicative Not Tested Test No ( 16 ) Copyright 2015 CTS, Inc

17 Table 1 - Item 1 Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed U3J3HU / Indicative / Indicative Kastle-Meyer Test Acid Phosphatase Saliva scopic UAYML8 / Indicative test Test -Semen scopy -Saliva UAZKRV / Indicative Not Tested UF9YH8 / Indicative o-tol UHA7HB / Indicative UPT6GW / Indicative / Indicative Visual examination amylase UPU6B6 Not Tested / Indicative Inconclusive AMYLASE PLATE MICRO V4BH9W / Indicative V7F8B6 / Indicative / Indicative O-TOL V9Z287 / Indicative VKV7HR / Indicative Tetrabase Test No ( 17 ) Copyright 2015 CTS, Inc

18 Table 1 - Item 1 Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed VM2TTB / Indicative Amylase Diffusion W4LNZR / Indicative Amylase with radial diffusion exam WBNQ8D / Indicative WF2Q7P / Indicative TEST Rsid saliva H+E stain Other Tests Performed Urine WM2X2R / Indicative PTMB Acid Phosphatase () Slight yellowish stain present. microscopic (micro) Other Tests Performed Urine - Creatinine WXGZ8Q / Indicative / Indicative K-M Saliva XD83L9 / Indicative Not Tested Not competent in saliva testing XHZBMU Not Tested / Indicative Not Tested XJC9UK / Indicative / Indicative XXUUMQ / Indicative / Indicative Test No ( 18 ) Copyright 2015 CTS, Inc

19 Table 1 - Item 1 Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed Y28ZFH / Indicative / Indicative Not Tested This laboratory does not currently offer saliva testing Y8GAQK / Indicative Inconclusive Amylase - Xmas tree stain Other Tests Performed Urine - Creatinine Urine - Urease Fecal Material - Urobilinogen Y8WHUU / Indicative Not Tested Y9VLM6 Phenolphthalein SaligaE YBDZQ6 / Indicative Not Tested Visual scopic exam - spermatazoa[sic] identified tube test not accredited YLWGGQ Not Tested / Indicative Not Tested Not a brown stains[sic] scopic YMCGYU / Indicative Not Tested -Semen Not tested for saliva since item tested positive for semen. YN3XWA / Indicative Hemastix 60 secs 8½ mins YQT4CZ Not Tested / Indicative Inconclusive No red brown staining Radial Diffusion YTUK / Indicative Not Tested Test No ( 19 ) Copyright 2015 CTS, Inc

20 Table 1 - Item 1 Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed YZDAA4 Phenolphthalein SaligaE ZCTE3K / Indicative Visual scopic ZKJZ9J / Indicative Seratec ZRYNXE / Indicative / Indicative PHADEBAS (TUBE METHOD) MICRO Other Tests Performed URINE, TEST PERFORMED CREATININE - FAINT ZYFE47 Not Tested / Indicative No red discoloration Amylase Diffusion Response Summary for Table 1 - Item 1 Blood Semen Saliva /Indicative Inconclusive Not Tested (NT) Test No ( 20 ) Copyright 2015 CTS, Inc

21 Screening Conclusions & Test(s) Performed Indicate the overall conclusion from the results of the screening tests performed. Table 1 - Item 2 27CG2C Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed MICRO PHADEBAS 28BLGK Not Tested 2EMTZU / Indicative 2FHERZ / Indicative Semen Saliva 2PQVGK / Indicative 2VEVGY / Indicative 3FF4BN / Indicative 3MXG82 / Indicative Other Tests Performed Urine - Creatinine Urine - DMAC Faeces - Edelmanns 3P4YCN / Indicative -Saliva 4ZYB / Indicative Amylase (radial diffusion) Test No ( 21 ) Copyright 2015 CTS, Inc

22 Table 1 - Item 2 Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed 4BJLQG Not Tested 4HXUT4 Not Tested 4JVXRK / Indicative 68YCXV / Indicative OT 6HVCDK / Indicative Visual -Saliva 6PZ4XB / Indicative 6UWLGW / Indicative 6ZGMFL Not Tested Not Tested Swab was not red stained P-30 7M6VAR Not Tested / Indicative No visible stain observed Amylase 7MNLCK Not Tested Not Tested Swabs have no red stains scopic Per Agency's Policy 7XNELB / Indicative KPIC Test No ( 22 ) Copyright 2015 CTS, Inc

23 Table 1 - Item 2 Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed 8C6JGB / Indicative Other Tests Performed Urine - Creatinine Feces - Urobilinogen 8NUMDA Not Tested This laboratory does not perform testing for saliva. 8PP7HW Not Tested / Indicative test tube test 8XLW8D / Indicative Visual -Saliva 8YD3JW Not Tested / Indicative No visible blood-like staining observed, therefore no presumptive test for blood performed : x4 negative : x4 positive 9B677N / Indicative 9GQWTH / Indicative 9HLHJN / Indicative 9JH363 / Indicative Press 9Q3XCX / Indicative AMYLASE DIFFUSION 9UE4NY Not Tested / Indicative Visual exam - no staining Amylase Diffusion observed Test No ( 23 ) Copyright 2015 CTS, Inc

24 Table 1 - Item 2 Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed 9W3CXZ / Indicative press AWHZRY / Indicative B2DAF7 / Indicative Hemastix - neg 60 secs Hematrace - neg 10 mins - neg 60 secs - neg p30 - neg 10 mins - pos 1 min B33FWW Not Tested / Indicative Not tested: No red-brown staining observed on the Items. BA8DNA / Indicative Visual BHXXRM Not Tested / Indicative Not tested due to lack of reddish-brown color on swabs. Item 2 AnB:, Item 2 CnD:, Item 2 AnB: Item 2 CnD: BMUKKB / Indicative Seratec BNKQXU / Indicative Hematrace BU8H89 Not Tested BYM4XV Not Tested / Indicative no apparent bloodstain Amylase diffusion observed Test No ( 24 ) Copyright 2015 CTS, Inc

25 Table 1 - Item 2 Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed C4UYB4 Not Tested Saliva testing is not offered as a service in this laboratory. CGLJZ8 / Indicative Amylase Other Tests Performed Urine - Creatinine Urine - DMAC Fecal Material - Urobilinogen DL73AZ / Indicative Press EB38QV / Indicative PHADEBAS MICRO EB9GR9 / Indicative TMB Saliva Field Kit EFF3F6 / Indicative (Seri ) Amylase () Other Tests Performed Urine - Creatinine, DMAC Fecal Material - Urobilinogen ERDV3B / Indicative EVPZDB Not Tested / Indicative Not Tested White scene or kit swabs are not tested for blood in this lab (one (1) head observed) Saliva testing not being done in this lab at this time EXGQ74 / Indicative (S) Amylase (ABA) (Xmas) Other Tests Performed Urine - Creatinine Fecal Material - Urobilinogen Test No ( 25 ) Copyright 2015 CTS, Inc

26 Table 1 - Item 2 Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed F42C9M / Indicative Phenolphthalein SaligaE FVMNEL / Indicative O-Tol FXB6NT / Indicative G74RZB / Indicative G8XFEZ Inconclusive Not Tested TMB Haemochromogen scopic Other Tests Performed Ouchterlony (Human origin) G9NLVQ / Indicative PHADEBAS PER TEST GFC6FU / Indicative GPVJEP / Indicative Visual exam Paper H&E Slide H&E slide GV43GN Not Tested / Indicative no staining on swab Amylase diffusion GZXZM8 Not Tested Not Tested *swabs were not red stained Test No ( 26 ) Copyright 2015 CTS, Inc

27 Table 1 - Item 2 Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed H7J77Z / Indicative (Map) a-amylase Other Tests Performed Urine - Creatinine Fecal Material - Urobilinogen H9ADNB / Indicative HDYUYF Not Tested Abacard Hematrace Abacard p30 HEDJ2T / Indicative ABAcard ABAcard test HHBZEM Not Tested / Indicative A red stain was not observed. Amylase diffusion HQ63HJ / Indicative HWRXDW / Indicative Radial Diffusion J23ATA / Indicative -Semen Starch-Iodine Assay J94HFP Not Tested / Indicative Test No ( 27 ) Copyright 2015 CTS, Inc

28 Table 1 - Item 2 Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed JHVYAZ / Indicative Other Tests Performed Urine - Creatinine Feces - Urobilinogen JJM2UU / Indicative tube test Other Tests Performed Urine - DMAC JNFWZ9 / Indicative K-M -Saliva scopic sperm search Other Tests Performed Urine - Visual & DMAC Feces - Visual & scopy JPQ3UN / Indicative PHADEBAS PER TEST JRWLWQ Not Tested Visual Exam Fast Blue () () () () () Current protocol does not allow for Saliva testing on swabs. JTMRDG / Indicative -Saliva JYWP6A / Indicative Starch-iodine assay K6XJEV / Indicative K7UYQR / Indicative Visual KQ3XGF / Indicative ABAcard p30 radial diffusion (for nucleated epithelial cells) Test No ( 28 ) Copyright 2015 CTS, Inc

29 Table 1 - Item 2 Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed KUE3RF / Indicative O-TOL MICRO KXYWNG / Indicative ABAcard Saligae KZ3R7Y Not Tested LXURT6 / Indicative Visual exam M9CTN6 / Indicative Visual MED8A7 / Indicative O-Tol MEWRED Not Tested / Indicative Swabs were not tested for blood - does not display the characteristic reddish-brown stain associated with blood.,,,, Saliva Saliva Saliva Saliva MQAX88 / Indicative Starch-iodine MY39JM Not Tested Not Tested *saliva testing not performed in this lab MZHQHM Not Tested Visual Exam Fast Blue () p30 () Current protocol does not allow for Saliva testing on swabs. Test No ( 29 ) Copyright 2015 CTS, Inc

30 Table 1 - Item 2 Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed N3ZCLU / Indicative p30 KPIC N9PM3J Not Tested / Indicative No red-brown discoloration Amylase Diffusion N9PRB2 / Indicative NQ6YNH Not Tested / Indicative -Saliva -Semen P8P4F9 Not Tested / Indicative No reddish/brown stain observed during visual inspection. scopy Saliva PCGF7Y / Indicative Amylase Testing by Radial Diffusion PDCWKG / Indicative ABA Card SALIgAE PJ2EYR Not Tested Hematrace micro Testing not offered. PJ7RQU / Indicative Seratec PKWW3E / Indicative Visual Slide made (micro) Test No ( 30 ) Copyright 2015 CTS, Inc

31 Table 1 - Item 2 Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed PMLJF3 Not Tested / Indicative *NT because no red brown stain observed. PNF7UR / Indicative TMB (amylase) Other Tests Performed Human - Ouchterlony Faeces - Price (urobilin) QADXPH / Indicative Hemastix (TMB) 60 secs scopy 36 secs QP6VRD / Indicative BCIP Amylase Diffusion R9VBPE Not Tested RBKG8Q / Indicative P-30 KPIC RDTPGB / Indicative RNACNQ / Indicative TMB HaemaTrace[sic] Amylase Haemachromogen[sic] Other Tests Performed Faeces - Prices RNQTHJ Not Tested Visually Other Tests Performed DNA-analysis RVQ877 Not Tested / Indicative No visual apparent blood Amylase diffusion staining Test No ( 31 ) Copyright 2015 CTS, Inc

32 Table 1 - Item 2 Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed T2V4RR / Indicative Seratec T7LPCY Not Tested scopic No currently validated procedures for saliva test. TJBFHW / Indicative TKLJQE Not Tested / Indicative No stain consistent with blood Amylase Diffusion TX4EYX Not Tested U3J3HU / Indicative UAYML8 / Indicative Test Test -Saliva UAZKRV Not Tested Not Tested Swabs were not stained UF9YH8 / Indicative o-tol -semenogelin UHA7HB / Indicative UPT6GW / Indicative Visual examination amylase Test No ( 32 ) Copyright 2015 CTS, Inc

33 Table 1 - Item 2 Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed UPU6B6 Not Tested / Indicative AMYLASE PLATE MICRO V4BH9W / Indicative V7F8B6 / Indicative O-Tol V9Z287 / Indicative PHADEBAS VKV7HR / Indicative tetrabase VM2TTB Not Tested / Indicative No apparent discoloration to Amylase Diffusion swabs W4LNZR / Indicative Amylase with radial diffusion exam WBNQ8D / Indicative WF2Q7P / Indicative Rsid saliva Rsid semen Other Tests Performed Rsid Urine WM2X2R Not Tested / Indicative no visible dried red stain Acid Phosphatase () (amylase) present / Other Tests Performed Urine - Creatinine Test No ( 33 ) Copyright 2015 CTS, Inc

34 Table 1 - Item 2 Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed WXGZ8Q / Indicative K-M Saliva XD83L9 Not Tested Not competent in saliva testing XHZBMU Not Tested Not Tested no red stain observed XJC9UK / Indicative / Indicative Amylase XXUUMQ / Indicative Y28ZFH Not Tested This laboratory does not currently offer saliva testing Y8GAQK / Indicative Amylase Other Tests Performed Urine - Creatinine Fecal Material - Urobilinogen Y8WHUU Not Tested Y9VLM6 / Indicative Phenolphthalein SaligaE YBDZQ6 Not Tested Visual scopic exam - no spermatazoa[sic] tube test not accredited YLWGGQ Not Tested Not Tested Not brown stains scopic Test No ( 34 ) Copyright 2015 CTS, Inc

35 Table 1 - Item 2 Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed YMCGYU / Indicative -Semen Starch-iodine test YN3XWA / Indicative Hemastix 60 secs 1 min 60 secs YQT4CZ Not Tested / Indicative No red brown staining Radial Diffusion YTUK Not Tested YZDAA4 / Indicative Phenolphthalein SaligaE ZCTE3K / Indicative Visual ZKJZ9J / Indicative Seratec ZRYNXE / Indicative PHADEBAS (TUBE METHOD) MICRO ZYFE47 Not Tested / Indicative No red discoloration Amylase Diffusion Response Summary for Table 1 - Item 2 Blood Semen Saliva /Indicative Inconclusive Not Tested (NT) Test No ( 35 ) Copyright 2015 CTS, Inc

36 Screening Conclusions & Test(s) Performed Indicate the overall conclusion from the results of the screening tests performed. Table 1 - Item 3 27CG2C Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed MICRO PHADEBAS 28BLGK / Indicative Not Tested Ouchterlony 2EMTZU / Indicative Not Tested Not required per policy 2FHERZ / Indicative micro Saliva ABACARD Hematrace Semen 2PQVGK / Indicative Other Tests Performed Sperm Search 2VEVGY / Indicative 3FF4BN / Indicative Hematrace 3MXG82 / Indicative Inconclusive tube test 3P4YCN / Indicative Not Tested Not Tested The swabs were heavily stained reddish-brown and there is no reason to believe semen would also be present on these swabs. The swabs were heavily stained reddish-brown and there is no reason to believe saliva would also be present on these swabs. Test No ( 36 ) Copyright 2015 CTS, Inc

37 Table 1 - Item 3 Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed 4ZYB / Indicative Amylase (radial diffusion) 4BJLQG / Indicative Not Tested Ouchterlony 4HXUT4 / Indicative Not Tested 4JVXRK / Indicative Not Tested Not tested per policy *Not Required 68YCXV / Indicative OT 6HVCDK / Indicative Visual -Saliva 6PZ4XB / Indicative Not Tested Case scenario did not require testing for saliva 6UWLGW / Indicative Hema Trace 6ZGMFL / Indicative Not Tested Kastle-Meyer 7M6VAR / Indicative,, Amylase 7MNLCK / Indicative Not Tested Kastle-Meyer Test scopic Per Agency's Policy Test No ( 37 ) Copyright 2015 CTS, Inc

38 Table 1 - Item 3 Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed 7XNELB / Indicative KPIC 8C6JGB / Indicative Modified Takayama 8NUMDA / Indicative Not Tested Not Tested Testing was not performed This laboratory does not due to case scenario. perform testing for saliva. 8PP7HW / Indicative Not Tested Inconclusive test tube test 8XLW8D / Indicative Visual -Saliva 8YD3JW / Indicative Not Tested Not Tested : Heavy blood-like staining observed, presumptive test performed, x4 positive As visible blood-like staining that gave an positive result was observed, no presumptive test for semen performed As visible blood-like staining that gave an positive result was observed, no presumptive test for saliva performed 9B677N / Indicative Not Tested Not tested per policy. 9GQWTH / Indicative Not Tested Not Tested Not applicable to case Not applicable to case scenario scenario 9HLHJN / Indicative Not Tested Per laboratory policy, only intimate samples are saliva tested. 9JH363 / Indicative Press ABACard Test No ( 38 ) Copyright 2015 CTS, Inc

39 Table 1 - Item 3 Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed 9Q3XCX / Indicative Not Tested Not Tested ABACARD HEMATRACE NOT TESTED DUE TO SCENARIO, LOCATION OF STAIN, AND POSITIVE TEST. NOT TESTED DUE TO SCENARIO, LOCATION OF STAIN, AND POSITIVE TEST. 9UE4NY / Indicative Amylase Diffusion 9W3CXZ / Indicative press ABAcard Hematrace AWHZRY / Indicative B2DAF7 / Indicative Not Tested Hemastix - pos 5 secs Hematrace - pos 2 mins - neg 60 secs - neg No indication in scenario of possibility of saliva B33FWW / Indicative Not Tested BA8DNA / Indicative Visual Spermatozoa () BHXXRM / Indicative Not Tested Item 3 AnB: Item 3 AnB:, Not tested due to swab type. Item 3 CnD: Item 3 CnD:, BMUKKB / Indicative Hematrace Seratec BNKQXU / Indicative Hematrace MICRO Test No ( 39 ) Copyright 2015 CTS, Inc

40 Table 1 - Item 3 Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed BU8H89 / Indicative Not Tested Ouchterlony Species Determination BYM4XV / Indicative Amylase diffusion C4UYB4 / Indicative Not Tested Saliva testing is not offered as a service in this laboratory. CGLJZ8 / Indicative Not Tested Not Tested Not tested based on case Not tested based on case Modified Takayama scenario scenario DL73AZ / Indicative Press ABACard EB38QV / Indicative PHADEBAS MICRO EB9GR9 / Indicative TMB Saliva Field Kit EFF3F6 / Indicative (Seri ) Amylase () Takayama ERDV3B / Indicative Not Tested Not routinely performed on positive stains EVPZDB / Indicative Not Tested Not Tested Brown or red stained scene swabs are not tested for semen in this lab Saliva testing not being done in this lab at this time Test No ( 40 ) Copyright 2015 CTS, Inc

41 Table 1 - Item 3 Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed EXGQ74 / Indicative Mod Taka (S) (ABA) (Xmas) Amylase F42C9M Phenolphthalein SaligaE FVMNEL / Indicative O-Tol Hematrace FXB6NT / Indicative G74RZB / Indicative Not Tested Not Tested No information to indicate that the human bloodstain on the wall would contain semen and no specific request for this testing No information to indicate oral contact and no specific request for this testing G8XFEZ / Indicative Not Tested TMB Haemochromogen G9NLVQ / Indicative Not Tested GFC6FU / Indicative ABA GPVJEP / Indicative Visual exam Paper H&E Slide GV43GN / Indicative Amylase diffusion Test No ( 41 ) Copyright 2015 CTS, Inc

42 Table 1 - Item 3 Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed GZXZM8 / Indicative Not Tested H7J77Z / Indicative Not Tested Not Tested Analyst's discretion (based on Analyst's discretion (based on Takayama scenario provided). scenario provided). H9ADNB / Indicative Not Tested The swabs were very dark and there was no evidence of dilution HDYUYF / Indicative Not Tested Abacard Hematrace Abacard p30 HEDJ2T / Indicative test ABAcard HHBZEM / Indicative Hematrace Amylase diffusion HQ63HJ / Indicative Hematrace HWRXDW / Indicative Radial Diffusion J23ATA / Indicative Not Tested -Semen HO J94HFP / Indicative TMB Test No ( 42 ) Copyright 2015 CTS, Inc

43 Table 1 - Item 3 Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed JHVYAZ / Indicative Modified Takayama JJM2UU / Indicative tube test Other Tests Performed Urine - DMAC JNFWZ9 / Indicative Not Tested Not Tested K-M No scene information was provided that made it seem likely this stain would contain more than one body fluid No scene information was provided that made it seem likely this stain would contain more than one body fluid JPQ3UN / Indicative Not Tested JRWLWQ / Indicative Not Tested Hemochromogen () Fast Blue (-ve) () Current protocols do not allow for Saliva testing on swabs. JTMRDG / Indicative -Blood -Saliva JYWP6A / Indicative Not Tested Hexagon OBTI K6XJEV / Indicative Inconclusive K7UYQR / Indicative Inconclusive Visual KQ3XGF / Indicative Takayama ABAcard p30 radial diffusion Test No ( 43 ) Copyright 2015 CTS, Inc

44 Table 1 - Item 3 Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed KUE3RF / Indicative O-TOL HEMATRACE MICRO KXYWNG / Indicative ABAcard Saligae KZ3R7Y / Indicative Not Tested Ouchterlony scopic LXURT6 / Indicative Visual exam M9CTN6 / Indicative Visual MED8A7 / Indicative O-Tol Hematrace MEWRED / Indicative Not Tested,,,, Did not test for saliva based on case scenario. MQAX88 / Indicative Not Tested Hexagon OBTI MY39JM / Indicative Not Tested *saliva testing not performed in this lab MZHQHM / Indicative Not Tested Hemochromogen Fast Blue () Current protocols do not allow for Saliva testing on swabs. Test No ( 44 ) Copyright 2015 CTS, Inc

45 Table 1 - Item 3 Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed N3ZCLU / Indicative p30 KPIC N9PM3J / Indicative Amylase Diffusion N9PRB2 / Indicative Not Tested positive stains are not routinely tested for saliva NQ6YNH / Indicative Not Tested -Blood -Semen P8P4F9 / Indicative Saliva scopy PCGF7Y / Indicative Not Tested Not Tested Scenario didn't indicate the Scenario didn't indicate the need for this testing. need for this testing. PDCWKG / Indicative ABA Card SALIgAE PJ2EYR / Indicative Not Tested Testing not offered PJ7RQU / Indicative Hematrace Seratec PKWW3E / Indicative Not Tested Not Tested Visual Area recovered - wasn't described as sex swab Area recovered from - was deemed unnecessary (& not dilute looking) Test No ( 45 ) Copyright 2015 CTS, Inc

46 Table 1 - Item 3 Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed PMLJF3 / Indicative Not Tested Not Tested Item 3 was not tested for semen because of the location of the stain. Item 3 was not tested for saliva because of the location of the stain. PNF7UR / Indicative TMB Haemochromogen (amylase) QADXPH / Indicative Hemastix (TMB) Test scopy a-amylase QP6VRD / Indicative / Indicative BCIP Amylase Diffusion Hematrace R9VBPE / Indicative Not Tested Scenario specific - case details does not declare biting nor oral contact RBKG8Q / Indicative P-30 KPIC RDTPGB / Indicative RNACNQ / Indicative TMB Amylase RNQTHJ / Indicative Not Tested OBTI RVQ877 / Indicative Amylase diffusion Test No ( 46 ) Copyright 2015 CTS, Inc

47 Table 1 - Item 3 Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed T2V4RR / Indicative Hematrace Seratec T7LPCY / Indicative Not Tested scopic No currently validated procedures for saliva test. TJBFHW / Indicative Not Tested NT (Per Policy) - Item not eligible for saliva testing TKLJQE / Indicative Amylase Diffusion TX4EYX / Indicative Not Tested U3J3HU / Indicative Not Tested Per Policy; not required to test UAYML8 / Indicative Test -Blood Test -Saliva UAZKRV / Indicative Not Tested P-30 UF9YH8 / Indicative Hematrace -semenogelin UHA7HB / Indicative UPT6GW / Indicative amylase Test No ( 47 ) Copyright 2015 CTS, Inc

48 Table 1 - Item 3 Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed UPU6B6 / Indicative Not Tested Not Tested V4BH9W / Indicative Not Tested Case scenario & lab policy/procedures V7F8B6 / Indicative O-Tol V9Z287 / Indicative PHADEBAS VKV7HR / Indicative Tetrabase VM2TTB / Indicative Amylase Diffusion Hematrace W4LNZR / Indicative Amylase with radial diffusion exam WBNQ8D / Indicative WF2Q7P / Indicative Rsid Saliva Hematrace Rsid Semen Other Tests Performed Rsid Urine WM2X2R / Indicative PTMB Acid Phosphatase () (Amylase) Other Tests Performed Urine - Creatinine Fecal - Fecal - Urobilinogen Test No ( 48 ) Copyright 2015 CTS, Inc

49 Table 1 - Item 3 Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed WXGZ8Q / Indicative Not Tested K-M Per policy, item does not meet criteria to be tested for this body fluid - therefore not required to test. XD83L9 / Indicative Not Tested Not competent in saliva testing XHZBMU / Indicative Not Tested XJC9UK / Indicative XXUUMQ / Indicative Not Tested Saliva test not performed on this item per laboratory policy - not required to test Y28ZFH / Indicative Not Tested Not Tested Testing not performed due to the probative value of the sample based on the scenario given. This laboratory does not offer saliva testing. Y8GAQK / Indicative Amylase Modified Takayama Y8WHUU / Indicative Not Tested Ouchterlony - Human Y9VLM6 Phenolphthalein SaligaE YBDZQ6 / Indicative Not Tested Visual scopic exam - no spermatazoa[sic] tube test not accredited Test No ( 49 ) Copyright 2015 CTS, Inc

50 Table 1 - Item 3 Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed YLWGGQ / Indicative Not Tested Kastle-Meyer Test scopic YMCGYU / Indicative Not Tested -Semen Hexagon OBTI YN3XWA / Indicative Hemastix 5 secs 8½ mins Hematrace 20 secs 60 secs YQT4CZ / Indicative Not Tested Not Tested No senario[sic] to indicate No scenario to indicate other other bodily fluids present bodily fluids present YTUK / Indicative Not Tested Ouchterlony (human) YZDAA4 Phenolphthalein SaligaE ZCTE3K / Indicative Visual scopic ZKJZ9J / Indicative Hematrace Seratec ZRYNXE / Indicative Inconclusive MICRO CHOLINE PHADEBAS (TUBE METHOD) ZYFE47 / Indicative Amylase Diffusion Hematrace Test No ( 50 ) Copyright 2015 CTS, Inc

51 Response Summary for Table 1 - Item 3 Blood Semen Saliva /Indicative Inconclusive Not Tested (NT) Test No ( 51 ) Copyright 2015 CTS, Inc

52 Screening Conclusions & Test(s) Performed Indicate the overall conclusion from the results of the screening tests performed. Table 1 - Item 4 27CG2C Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed MICRO PHADEBAS 28BLGK, Not Tested / Indicative Not Tested 2EMTZU Not Tested / Indicative Not Tested Not required per policy Not required per policy 2FHERZ Not Tested / Indicative Swabs were not stained micro Saliva red-brown 2PQVGK / Indicative Other Tests Performed Sperm Search 2VEVGY / Indicative / Indicative 3FF4BN / Indicative 3MXG82 / Indicative Inconclusive 3P4YCN Not Tested / Indicative Not Tested Light yellow staining was noted on each swab; no reason to test for blood. Semen was detected and there is no reason to believe saliva would also be present on these swabs. Test No ( 52 ) Copyright 2015 CTS, Inc

53 Table 1 - Item 4 Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed 4ZYB Not Tested / Indicative No characteristic staining observed, would not test based on staining & scenario Amylase (radial diffusion) 4BJLQG / Indicative Not Tested 4HXUT4 / Indicative Not Tested 4JVXRK Not Tested / Indicative Not Tested No reddish-brown stain. Not tested per policy *Not Required Not tested per policy *Not Required 68YCXV / Indicative OT 6HVCDK / Indicative Visual -Saliva micro 6PZ4XB Not Tested / Indicative Not Tested Case scenario did not require testing for blood, absence of red-brown staining Case scenario did not require testing for saliva 6UWLGW / Indicative / Indicative 6ZGMFL Not Tested / Indicative Not Tested Swab not red stained 7M6VAR Not Tested / Indicative no visible stain observed ACP, Amylase 7MNLCK / Indicative Not Tested Swabs have no red stains scopic Per Agency's Policy Test No ( 53 ) Copyright 2015 CTS, Inc

54 Table 1 - Item 4 Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed 7XNELB / Indicative KPIC 8C6JGB / Indicative 8NUMDA Not Tested / Indicative Not Tested Testing was not performed due to case scenario. This laboratory does not perform testing for saliva. 8PP7HW Not Tested Inconclusive test tube test 8XLW8D / Indicative Visual -Saliva 8YD3JW Not Tested / Indicative No visible blood-like staining observed, therefore no presumptive test for blood performed : x4 positive : 2+ heads & tails, negative for NECs : x4 negative 9B677N / Indicative Not Tested Not tested per policy. 9GQWTH Not Tested / Indicative Not Tested No staining consistent with Not applicable to case scenario blood observed 9HLHJN / Indicative Not Tested Per laboratory policy, only intimate swabs from the vaginal area are saliva tested. 9JH363 / Indicative Press Test No ( 54 ) Copyright 2015 CTS, Inc

55 Table 1 - Item 4 Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed 9Q3XCX Not Tested / Indicative Not Tested NOT TESTED DUE TO LOCATION OF SWABBING, NO VISIBLE STAINING, AND POSITIVE SEMEN TEST. MICRO NO TESTING DUE TO POS INDICATION OF SEMEN. 9UE4NY Not Tested / Indicative Visual exam - no staining Amylase Diffusion observed 9W3CXZ / Indicative press Semiquant AWHZRY / Indicative B2DAF7 Inconclusive / Indicative Hemastix - wk pos 25 secs. Did not confirm presence of blood as semen positive on victim. - pos 8 secs - pos - neg 8.5 mins B33FWW Not Tested / Indicative Not tested: No red-brown staining observed on the Items. BA8DNA / Indicative Visual Spermatozoa () BHXXRM Not Tested / Indicative Not tested due to lack of reddish-brown color on swabs. Item 4 AnB:, Item 4 CnD:, Item 4 AnB: Item 4 CnD: BMUKKB / Indicative Seratec Test No ( 55 ) Copyright 2015 CTS, Inc

56 Table 1 - Item 4 Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed BNKQXU / Indicative / Indicative HEMATRACE MICRO BU8H89 / Indicative Not Tested BYM4XV Not Tested / Indicative no apparent bloodstain observed Amylase diffusion C4UYB4 Not Tested / Indicative Not Tested No red/brown staining was visualized on the samples; therefore, blood testing was not performed. Saliva testing is not offered as a service in this laboratory. CGLJZ8 / Indicative Amylase DL73AZ / Indicative Press EB38QV / Indicative PHADEBAS MICRO EB9GR9 / Indicative TMB Saliva Field Kit EFF3F6 / Indicative (Seri ) Amylase () (Xmas Stain) ERDV3B Not Tested / Indicative Not routinely performed on DRS-negative stains Test No ( 56 ) Copyright 2015 CTS, Inc

57 Table 1 - Item 4 Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed EVPZDB Not Tested / Indicative Not Tested White scene or kit swabs are Saliva testing not being done in not tested for blood in this lab this lab at this time EXGQ74 / Indicative Inconclusive (S) (ABA) (Xmas) Amylase F42C9M Phenolphthalein SaligaE FVMNEL Not Tested / Indicative Not applicable to case history FXB6NT / Indicative G74RZB / Indicative Not Tested No information to indicate oral contact by suspect and semen is present in this stain. G8XFEZ / Indicative / Indicative Not Tested TMB scopic G9NLVQ / Indicative PHADEBAS PER TEST GFC6FU / Indicative GPVJEP / Indicative Visual Exam Paper H&E Slide GV43GN Not Tested / Indicative no staining on swab Amylase diffusion Test No ( 57 ) Copyright 2015 CTS, Inc

58 Table 1 - Item 4 Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed GZXZM8 Not Tested / Indicative Not Tested *swabs were not red stained H7J77Z Not Tested / Indicative Analyst's discretion (based on (Map) a-amylase scenario provided). (Xmas) H9ADNB / Indicative / Indicative HDYUYF / Indicative / Indicative Not Tested Abacard Hematrace Abacard p30 HEDJ2T / Indicative test HHBZEM Not Tested / Indicative A red stain was not observed. Amylase diffusion HQ63HJ / Indicative / Indicative HWRXDW / Indicative Radial Diffusion J23ATA / Indicative Not Tested -Semen J94HFP Not Tested / Indicative JHVYAZ / Indicative Test No ( 58 ) Copyright 2015 CTS, Inc

59 Table 1 - Item 4 Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed JJM2UU / Indicative Inconclusive tube test Other Tests Performed Urine - DMAC JNFWZ9 Not Tested / Indicative Not Tested No visual indication that blood was present scopic sperm search No senario[sic] information provided that supports likelihood of a mixed semen/saliva stain JPQ3UN / Indicative PHADEBAS PER TEST JRWLWQ / Indicative Not Tested Visual Exam Fast Blue (+ve) () Current protocols do not allow (+ve) for Saliva testing on swabs. JTMRDG / Indicative -Semen scopy -Saliva JYWP6A / Indicative Not Tested K6XJEV / Indicative / Indicative K7UYQR / Indicative / Indicative Visual KQ3XGF / Indicative Inconclusive ABAcard p30 radial diffusion KUE3RF / Indicative O-TOL MICRO Test No ( 59 ) Copyright 2015 CTS, Inc

60 Table 1 - Item 4 Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed KXYWNG / Indicative ABAcard Saligae KZ3R7Y / Indicative Not Tested scopic LXURT6 / Indicative Visual exam M9CTN6 / Indicative Visual MED8A7 / Indicative O-Tol MEWRED Not Tested / Indicative Swabs were not tested for blood - does not display the characteristic reddish-brown stain associated with blood.,,,, Saliva Saliva Saliva Saliva MQAX88 / Indicative Not Tested MY39JM / Indicative Not Tested *saliva testing not performed in this lab MZHQHM / Indicative Not Tested Hemastix Fast Blue () Current protocols do not allow for Saliva testing on swabs. Other Tests Performed Contact DNA - Sent for DNA analysis N3ZCLU / Indicative Inconclusive p30 KPIC Test No ( 60 ) Copyright 2015 CTS, Inc

61 Table 1 - Item 4 Blood Conclusion Semen Conclusion Saliva Conclusion Tests Performed Tests Performed Tests Performed N9PM3J Not Tested / Indicative No red-brown discoloration Amylase Diffusion N9PRB2 Not Tested / Indicative Not Tested stains without any visible red/brown color are not routinely tested for blood (sperm search) semen positive stains are not routinely tested for saliva NQ6YNH Not Tested / Indicative -Saliva P8P4F9 Not Tested / Indicative No reddish/brown stain observed during visual inspection. scopy Saliva PCGF7Y Not Tested / Indicative no red-brown staining Amylase with Radial Diffusion PDCWKG / Indicative ABA Card SALIgAE PJ2EYR Not Tested / Indicative Not Tested Not tested based on staining. Testing not offered. PJ7RQU / Indicative Seratec PKWW3E / Indicative Inconclusive Visual Slide made () PMLJF3 Not Tested / Indicative / Indicative Item 4 was not tested for blood because of the location and color of the stain. Test No ( 61 ) Copyright 2015 CTS, Inc