Key words : Verotoxin-producing Escherichia coli, VTEC, EHEC, imported meat

Transcription

1 Key words : Verotoxin-producing Escherichia coli, VTEC, EHEC, imported meat

2

3 Table 2 Amounts of VT1, VT2 and VT2vh produced by the E. coli strains isolated from the beef and pork * Figure in parenthesis indicates an estimated value for VT2vh Table 3 Biochemical characteristics of the E. coli starains isolated from the beef and pork Table 1 Biological assay of toxin produced by the E. coli strains isolated from the beef and pork

4

5 that is immunologically unrelated to Shiga toxin. Microb. Pathog., 3 : 21-30, ) Marques, L.R.M., Peiris, J.S.M., Cryz, S.J. & O'Brien, A.D.: Escherichia coli strains iso- from pigs with edema disease produce lated a varient of Shiga-like toxin II. FEMS Microb. Lett., 44 : 33-38, ) Oku, Y., Yutsudo, T., Hirayama, T., O'Brien, A.D. & Takeda, Y. Purification and some properties of a Vero toxin from a human strain of Escherichia coli that is immunologically related to Shiga-like toxin II (VT2). Microb. Pathog., 6 : , ) Pai, C.H., Ahmed, N., Lior, H., Johnson, W.M., 1) Riley, L.W., Remis, R.S., Helgerson, S.D., McGee, H.B., Wells, J.G., Davis, B.R., Hebert, R.J., Olcott, E.S., Johnson, L.M., Hargrett, N. T., Blake, P.A. & Cohen, M.L. : Hemorrhagic colitis associated with a rare Escherichia coli serotype. New Engl. J. Med., 308: , ) Karmali, M.A. : Infection by Verocytotoxinproducing Escherichia coll. Clin. Microbiol. Rev., 2 : 15-38, ) Noda, M., Yutsudo, T., Nakabayashi, N., Hirayama, T. & Takeda, Y.: Purification and some properties of Shiga-like toxin from Escherichia coli o157 : H7 that is immunologically identical to Shiga toxin. Microb. Pathog., 2: , ) Takao, T., Tanabe, T., Hong, Y.M., Shimonishi, Y., Kurazono, H., Yutsudo, T., Sasakawa, C., Yoshikawa, M. & Takeda, Y.: Indentify of molecular structure of Shiga-like toxin 1(VT1) from Escherichia coli o157: H7 with that of Shiga toxin. Microb. Pathog., 5: 357 5) Jackson, M.P., Neill, R.J., O'Brien, A.D., Holmes, R.K. & Newland, J.W. : Nucleotide sequence analysis and comparisons of the structural genes for Shiga-like toxin I and Shigalike toxin II encoded by bacteriophages from Escherichia coli 933. FEMS Microb. Lett., 44 : , ) Yutsudo, T., Nakabayashi, N., Hirayama, T. & Takeda, Y.: Purification and some properties of a Vero toxin from Escherichia coli o157 : H7 Sims, H.V. & Woods, D.E. : Epidemiology of sporadic diarrhea due to verocytotoxinproducing Escherichia coli : A two-year pro- spective study. J. Infect. Dis., 157 : , ) Scotland, S.M., Rowe, B., Smith, H.R., Willshaw, G.A. & Gross, R.J.: Vero cytotoxin producing strains of Escherichia coli from children with haemolytic uraemic syndrome and thier detection by specific DNA probes. J. Med. Microbiol., 25 : , ) Scotland, S.M., Day, N.P. & Rowe, B.: Production of a cytotoxin affecting Vero cells by strains of Escherichia coli belonging to traditional enteropathogenic serogroups. FEMS Microb. Lett., 7 : 15-17, 1980.

6 Y.: Development of a highly sensitive bead- meats and poultry. Appl. Environ. Microbiol., ELISA to detect bacterial protein toxins. Microbiol. Immunol., 38 : , ) Mohammad, A., Peiris, J.S.M. & Wijewanta, E. 56 : , A.: Serotypes of verocytoxigenic Escherichia coli isolated from cattle and buffalo calf diarr- FEMS Microb. Lett., 35 : , hoea. 24) Ito, H., Terai, A., Kurazono, H., Takeda, Y. & Nishibuchi, M.: Cloning and nucleotide se- of Veto toxin 2 variant genes from quencing Escherichia coli 091: H21 isolated from a patient with the hemolytic uremic syndrome. Microb. Pathog., 8 : 47-60, ) Weinstein, D.L., Jackson, M.P., Samuel, J.E., Holmes, R.K. & O'Brien, A.D. : Cloning and sequencing of a Shiga-like toxin type II variant 20) Ewing, W.H. : Edwards and Ewing's from an Escherichia coli strain responsible for Identification of Enterobacteriaceae, 4th Ed., edema disease of swine. J. Bacteriol., 170 : 4223 Elsevier Science Publishing Co., New York, ) March, S.B. & Ratnam, S.: Sorbitol- MaConkey medium for detection of Escheri- 22) Doyle, M.P. & Schoeni, J.L. : Isolation of Escherichia coli 0157: H7 from retail fresh chira coli 0157: H7 associated with hemorrhagic colitis. J. Clin. Microbiol., 23 : , Isolation of Vero Toxin-Producing Escherichia coli (VTEC) from Imported Meats Hiroshi TANAKA, Chikara NISHIUCHI, Reiko KONDO & Mari KIMURA Iyo Public Health Center, Ehime Kiyoshi MEMIDA & Masatake KIKUCHI Ehime Prefectural Veterinary Diagnostic Laboratory Teizo TUSKAMOTO Osaka Prefectural Institute of Public Health Yuichi OKU Institute for Diagnostic Reagents, Nissui Pharmaceutical Co. Shinji YAMASAKI & Yoshifumi TAKEDA Department of Microbiology, Faculty of Medicine, Kyoto University One hundred and four samples of various imported meats obtained from the stores and supermarkets in Matsuyama-city and Iyo-city from November, 1987 to October, 1988 were examined and two strains of Vero toxin-producing Escherichia coli were isolated: one was from beef from Australia and the other was from pork from Canada. By bead-elisa, it was confirmed that the strain from beef produced Vero Toxin 1 (VT1) and Vero toxin 2 variant (VT2vh) and the strain from pork produced VT2vh. These results suggest that imported meats may be contaminated with VTEC and thus are possible sources of human VTEC infection, such as hemorrhagic colitis and hemolytic uremic syndrome.