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1 - ( ) - * :... (MDR) (ESBLs: Extended-Spectrum Beta Lactamases). :.. (CLSI:Clinical and Laboratory Standards Institute). ESBL. combined disk method ESBL : / / / / / B / ( /). / / combined disk method. ( ). ( ) ESBL ( ). B :. ESBL. ESBLs : // : -// : * bzamanzad@yahoo.com
2 /.. ) (. (Oxidation Fermentation) OF Triple. (Sugar Iron Agar) TSI ) - (. -. ( )..().().() (MDR).() Amp C ) DNA D2 (. ( ).( ) Multi-drug efflux...
3 / / ( Escherichia coli ATCC ( ). - ) (. ( /) ( /) ( /) ( ). ( ) ( ).... B (. - :. / ) B. ( Himedia Pseudomonas aeruginosa ATCC ESBLs: ) (Extended-Spectrum Beta Lactamases Combination Disk ) + (Method ) (.() ( Mast.. -. C. (ESBLs) ) Klebsiella pneumoniae ATCC
4 /.().. / / / B / / /. / /....().( ) / -/. (combination disk method). ESBL ESBL ( ) ( ). ESBL ( /) ( /).() Combined disk method ( ESBL (P</) ESBL.( ) ( ESBL / ESBL / ESBL
5 / /... ESBL. ESBL PCR. ESBL (-)..().(-) -/ / / ( ). (Starveta.t) B (). / /- /-.(-).( ) / /) ( ) (
6 /. :.. ( ) B.. References: 1.Yan JJ, Tsai SH, Chuang CL, et al. OXA-type beta-lactamases among extended-spectrum cephalosporin-resistant Pseudomonas aeruginosa isolates in a university hospital in southern Taiwan. J Microbiol Immunol Infect 2006; 39: S Shenoy, S Baliga, DR Saldanha, et al. Antibiotic sensitivity patterns of Pseudomonas aeruginosa strains isolated from various clinical specimens. Indian J Med Sci 2002; 56: Pirnay J, De Vos D, Cochez C, et al. Molecular epidemiology of Pseudomonas aeruginosa colonization in a burn unit: Persistence of a multidrug-resistant clone and a silver sulfadiazine-resistant clone. J Clin Microbiol 2003; 41: Chanawong A, M zali FH, Heritage J, et al. SHV-12, SHV-5, SHV-2a and VEB-1 extended-spectrum β- lactamases in Gramnegative bacteria isolated in a university hospital in Thailand. J Antimicrob Chemother 2001; 48: Prinsloo A, van Straten AMS, Weldhagen GF. Antibiotic synergy profiles of multidrugresistant Pseudomonas aeruginosa in a nosocomial environment. South Afr J Epidemiol Infect 2008; 23: Bonfiglio G, Carciotto V, Russo G, et al. Antibiotic resistance in Pseudomonas aeruginosa: an Italian survey. J Antimicrob Chemother 1998; 41: Geha DJ, Uhl JR, Gustaferro CA, et al. Multiplex PCR for Identification of Methicilin-Reesistant Staphylococci in the Clinical Laboratory. J Clin Microbiol 1994; 32: Knothe H, Shah P, Krcmery V, et al. Trasferable resistance to cefotaxime, cefoxitin, cefamandole and cefuroxime in clinical isolates of klebsiella pneumoniae and serratia marcescense. Infection 1983; 11: Vahdat K, Rezaie R, Gharibi O. Bacteriology of hospital-acquired infection and antibiotic resistance in a hospital university of Bushehr Port "Fatemeh Zahra (s)" in Iran South Med J 2005; 7: Lee S, Park YJ, Kim M, et al. Prevalence of Ambler class A and D ß-lactamases among clinical isolates of Pseudomonas aeruginosa in Korea. J Antimicrob Chemother 2005; 56: Strateva T, Ouzounova-Raykova V, Markova B, et al. Problematic clinical isolates of Pseudomonas aeruginosa from the university hospitals in Sofia, Bulgaria: current status of antimicrobial resistance and prevailing resistance mechanisms. J Med Microbiol 2007; 56: Cavallo JD, Fabre R, Leblanc F, et al. Antibiotic susceptibility and mechanism of B- lactam resistance in 1310 strain of Pseudomonas aeruginosa: a French multicentre study (1996). J Antimicrob Chemother 2000; 46: Mirsalehian A, Feizabadi M, Akbari Nakhjavani F, et al. Prevalence of extended spectrum beta lactamases among strains of Pseudomonas aeruginosa isolated from burn patients (Persian). Tehran Uni Med J 2008; 66: Carmeli Y, Troillet N, Eliopoulos GM et al. Emergence of antibiotic-resistant Pseudomonas aeruginosa: comparison of risks associated with different antipseudomonal agents. Antimicrob Agents Chemother 1999; 43, Srikumar R, Kon T, Gotoh N et al. Expression of Pseudomonas aeruginosa multidrug Efflux Pumps MexA-MexB-OprM and MexC-MexD-OprJ in a multidrugsensitive Escherichia coli starin. Antimicrob Agents Chemother 1998; 42,
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