Report on External Quality Assessment Scheme Survey no. 253 Autoimmun Diseases 02 March 2015
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1 Report on External Quality Assessment Scheme Survey no. 253 Autoimmun Diseases 2 March 15 INSTAND e.v. Dr. M. Blüthner Prof. Dr. Hans-Peter Seelig issued by: Instand e.v. Gesellschaft zur Förderung der Qualitätssicherung in medizinischen Laboratorien e.v. Düsseldorf,
2 EQA-scheme consultant Dr. Martin Blüthner MVZ Labor PD Dr. Volkmann u. Kollegen Kriegsstr. 99 D Karlsruhe Tel. +49 () Fax +49 () bluethner@laborvolkmann.de Assistant EQA-scheme consultant Professor Dr. Hans-Peter Seelig Kriegsstr. 99 D Karlsruhe Tel. +49 () Fax +49 () labor@laborvolkmann.de Organisation and Logistics: INSTAND e.v. Ubierstr. 223 Düsseldorf Tel. +49 () Fax +49 () instand@instand-ev.de Report on Instand e.v. EQAS 253 March 15
3 INSTAND e. V. Ubier-Str. / PF / 93 Düsseldorf EQC 253 Autoimmune Disease 2 Antibodies against mitochondria, microsomes, smooth muscle actin and parietal cells EQC march 15 A summary of the results obtained from all participants including a graphical representation of the results is given in the attached tables and figures. For the evaluation of the IIF-tests only the reported titers were considered. For titers below the reference range, was entered. For the evaluation of other methods (Elisa, RIA, other quantitative methods) the quantitative criteria ( - 4), or qualitative criteria or 1 (western-, line-, dotblot) were applied. The sample was serum from a 35-yr old female donor, which showed antibodies against parietal cells in IIFT. With specific tests antibodies against H + -K + -ATPase were detectable. No other antibodies against mitochondria, smooth muscle, actin, or LKM-1 could be found. The material was used undiluted. The participants had no difficulties with this sample. The sample was serum from a 53-yr old female donor, which showed antibodies against parietal cells in IIFT. With specific tests antibodies against H + -K + -ATPase were detectable. No other antibodies against mitochondria, smooth muscle, actin, or LKM-1 could be found. The material was used undiluted. The participants had no difficulties with this sample. gez. Dr. M. Blüthner Kriegsstraße Karlsruhe
4 Autoimmune diseases 2 March Participants 223 Test Certificates anti-mitochondria IIFT 19 participants Results received True results % 176 anti-mitochondria quantitative participants Results received 33 83% 39 True results 32 97% 38 anti-mitochondria qualitative 82 participants Results received True results 79 98% 8 anti-lkm 1 IIFT 1 participants Results received 1 1% 139 True results % 133 anti-lkm 1 quantitative 32 participants Results received 32 1% 32 True results 32 1% 32 anti-lkm 1 qualitative 96 participants Results received 96 1% 95 True results 96 1% 95 anti-smooth muscle IIFT 176 participants Results received 176 1% 175 True results % 164 anti-actin IIFT 117 participants Results received 117 1% 117 True results % 114 anti-actin quantitative 14 participants Results received 14 1% 14 True results 13 93% 13 anti-parietal cells IIFT 149 participants Results received True results 132 9% 136 anti-h+/k+-atpase quantitative 44 participants Results received 44 1% 44 True results 43 98% 43 93% 98% 97% 1% 98% 96% 1% 1% 1% 94% 1% 97% 1% 93% 1% 91% 1% 98% % % % % % 43 98% Prof. H.P. Seelig - 1 -
5 Autoimmune diseases 2 March anti-mitochondria IIFT 1 anti-mitochondria IIFT anti-mitochondria quantitative 1 anti-mitochondria quantitative anti-mitochondria qualitative 1 1 anti-mitochondria qualitative anti-lkm 1 IIFT 1 anti-lkm 1 IIFT Prof. H.P. Seelig - 2 -
6 Autoimmune diseases 2 March anti-lkm 1 quantitative 1 anti-lkm 1 quantitative anti-lkm 1 qualitative 1 1 anti-lkm 1 qualitative anti-smooth muscle IIFT 1 anti-smooth muscle IIFT anti-actin IIFT 1 anti-actin IIFT Prof. H.P. Seelig - 3 -
7 Autoimmune diseases 2 March anti-actin quantitative 1 anti-actin quantitative anti-parietal cells IIFT 1 anti-parietal cells IIFT anti-h+/k+atpase quantitative 1 anti-h+/k+atpase quantitative Prof. H.P. Seelig - 4 -
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