PROCEEDINGS OF THE FIFTII INTERNATIONAL SYMPOSIUM ON CORONAVIRUSES. Held September 13-18, 1992, in Chantilly France

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1 PROCEEDINGS OF THE FIFTII INTERNATIONAL SYMPOSIUM ON CORONAVIRUSES Held September 13-18, 1992, in Chantilly France

2 INDEX Adenovirus expression, ofprcv S protein, 469 oftgev S gene fragments, 455 A59 virus, see Murine hepatitis virus, A59 strain Amantadine effect on MHV replication, 117 Aminopeptidase N I CD13, cell receptor for HCV 229E, 261 TGEV and PRCV, 293 Antibody see also Monoclonal antibody Fe binding to BCV S protein, 183 response in chicken immunized with mv structural proteins, 449 to MHV non-structural proteins, 221 to TGEV S gene fragments, 455 Antibody neutralization anti-s protein antibodies, 401 Arterivirus Equine Arteritis virus characterization of a viral cysteine protease, 227 genomic organization, 235 structural proteins, 245 Astrocytes see also Glial celis as IL6 producing cells, 443 Avian infectious bronchitis virus see infectious bronchitis virus Baculovirus expression of NS2 protein of BCV, 69 Bovine coronavirus BCV Fc binding by S protein, 183 non-structural protein NS2, 69 Canine coronavirus CCV sequence analysis, 3, 11 Cardiomyopathy, 365 Carcinoembryonic antigen, CEA CGM isoforms in susceptible and resistant mice, 267 CGM2 as receptor for MHV in the brain, 273 Receptor for MHV A59, 261 Cell-mediated immunity, adoptive transfer, 413, 425 CD4+ T cell clones, 413 cytotoxic CD8+ T cell, 431 cytotoxic T cell lines analysis of protein specificity, 419, 471 DTH reaction elicited by mv structural proteins, 449 Cell-mediated immunity (continued) phenotyping of T cell from the brain parenchyma, 437 regulation of PCA induction by T cell clones, 377 Central nervous system diseases see also demyelination see also encephalomyelitis 1L6 production by astrocytes and endothelial cells, 443 cytotoxic T cells, 407 immune response and CNS disease, 413, 419, 425,431,437,443 T cell-mediated cytotoxicity in MHV-JHM infections, 419 Cytokines IL6 in response to MHV JHM infection, 443 lub and TNF in response to MHV infection, 111 Defective-interfering particles, DI DI RNA transcription and translation, 137 leader-primed transcription analysis, 91 Demyelination see also Central nervous system disease immunopathogenesis induced by anti-s protein antibodies, 401 E.coli, expression in, ofeav cystein protease, 227 ofmhv non-structural proteins, 221 of BCV-NS2 protein, 69 Encephalitides, in BN and LEW rats, 431 Encephalomyelitis see also Central nervous system diseases encephalomyelitis and anti-s antibodies,401 in LEW rat, 413, 425 in BN and LEW rats, 437 in Wistar rats following HEV inoculation, 333 Endoplasmic reticulum retention signal, in FlPV ORF6b protein, 209 Epitopes cytotoxic T cell epitopes, 407 linear neutralizing epitopes, 455 on S protein, T cell epitopes, 419, 471 on N protein, T cell epitopes, 413, 419 Eucaryotic cells, expression in, of Aminopeptidase N, 293 of CGM isoforms, in BHK and SJL cells, 267 in COS cells,

3 Evolution arterivirus, torovirus and coronavirus relationships, 235 deletions in genome, 11,29,43 HCV-OC43 and BCV relationships, 61 TGEV and PRCV relationships, 35, 43 Expression see Baculovirus expression, see E.coli, expression in, see eukaryotic ceus, expression in, see Vaccinia virus expression Feline enteric coronavirus FECV non-structural ORF6 proteins, 11 Feline infectious peritonitis virus FIPV early death syndrome, 359 non structural protein ORF 6b, 209 Fusion induction by uncleaved S protein ofmhv, 165, 171 MHV-A59, fusion defective mutants, 157 MHV-IHM recombinant S protein, non fusogenic variant, 401 variation among MHV isolates, 157 Genes, see RNA Genome organisation CCV, 3 CCV and FECV, 11 comparative aspects in arteri-, toro-, and coronaviruses, 235 PEDV,55 TGEV, Taiwanese isolate TFl, 23 Genome stability MHV4 during persistent infections, 327 Glial cells see also Astrocytes growth of HCV 229E, 339 H 2, see Major histocompatibility complex Hemagglutinating encephalomyelitis virus, HEV S protein, 299 infection in Wistar rats, 333 Hemagglutinin-esterase protein HE acetylesterase activity, 299 HE defective mutants, 319 Hemagglutination by S protein, of BCV and HEV, 299 oftgev and lbv, 305 Human coronavirus OC43, HCV OC43 cell receptor, 285 sequence ofs and non-structural proteins, 61 Human coronavirus 229E, HCV 229E cell receptor, 261 growth in neuronal cells, 339 Humoral lmmunity see also Antibody, see also Monoclonal antibody, see also Antibody neutralization association with cell mediated immunity in MHV infections, 425 lmmunopathology FIPV and S protein, 359 role ofanti-s antibodies in demyelination, 401 Infectious bronchitis virus lbv positive sense subgenomic RNAs in lbv virions, 123 variant strain PL 84084, 395 Inm!l hybridization MHV IHM RNA in primate CNS tissue, 347 MHV-JHM route to CNS, 313 JHM, see Murine hepatitis virus, JHM strain LeaIerRNA antisense RNA, effect on trancription, 129 leader-body fusion site, 91 5' terminal sequence, 105 internal ORF, 105 Macrophage cell-line, regulation of cellular mrnas by MHV, 111 Macrophage procoagulant activity, PCA induction by MHV 3, 377, 385 inhibition by anti-prothrombinase MAb, 377 Major histocompatibility complex, MHC HLA class I molecules as receptor for HCV OC43,285 modulation of MHC class I molecules expression by MHV JHM, 111 isolation of peptides from MHC class I molecules, 407 Membrane protein M ofccv,3 interaction with S protein, 189 of lb V, induction of DTH, 449 ofpedv, 197 Monoclonal antibody, see also Neutralization resistant variants to S protein of lbv, 395 to M protein of PEDV, 197 to N protein of lbv, 395 to Prothrombinase, 377 Monoclonal antibody-resistant mutants, see Neutralization-resistant variants Multiple sclerosis presence of coronavirus RNA HCV 229E, 339 MHV-IHM, 353 Murine hepatitis virus-3, MHV-3 induction of macrophage PeA, 377, 385 Murine hepatitis virus-4, MHV-4, see Murine hepatitis virus, JHM strain Murine hepatitis virus, A59 strain cell receptor, 261, 267, 273 defective interfering RNA, 91 polymerase polyprotein, 215, 221 ts and thermolabile mutants, 143 Murine hepatitis virus, JHM strain defective-interfering RNA, 137 Fe binding by S protein, 183 growth in human neuronal cells, 353 infection of Owl monkeys, 347 replication in neuronal cells, 319 Murine hepatitis virus, Y strain, 371 Neuroattenuated MHV variants deletions in S protein,

4 Neutralization-resistant variants see also Antibody neutralization ofmhv strain Wbl, 171 Non-structural proteins BCV,69 CCV and FECV ORF 6b, 11 MHV-A59 non-structural protein p65, 221 HCV-OC43,61 TGEV, ORF 3a13b from virulent and attenuated strains, 29 Nucleocapsid protein N binding to phospholipids, 189 identification of CD4+ T cell sites, 413 ofccv,3 Organ tropism enterotropic MHV strain, 371 MHV-JHM,313 Peplomer see Spike protein S Peptides see also Antibody, see also Antibody neutralization ofmhv-a59 ORFla protein, 221 Persistent infection BCV, control by intraleader ORF, 105 of MHV in primary neural cultures, 157 of MHV4 in the CNS, 327 Polymerase, polypeptides, 215,221, 227 proteolytic processing of ORFI polyprotein ofmhv-a59, 215, 221 ofeav, 227 sequence data, 75 Polymerase chain reaction, PCR, for the characterization of TGEV strains, 29 RT-PCR for the detection of MHV4 in persistent infections, 327 HCV 229E in human brain, 339 Coronavirus RNA in human brain, 353 Porcine epidemic diarrhoea virus PEDV monoclonal antibody, 197 N protein, 49 Porcine respiratory coronavirus PRCV absence of cross protection between PRCV and TGEV, 463 cell receptor, 293 differentiation from TGEV, 43 Porcine transmissible gastroenteritis virus, see Transmissible gastroenteritis virus Prostaglandin E2, 385 Protective immune responses induced by fusogenic MHV JHM S protein, 401 induced by ts mutant ofmhv A59, 407 Rabbit coronavirus RbCV rabbit dilated cardiomyopathy, 365 Receptors, cell for HCV OC43, 285 for HCV 229E, 261 for MHV-A59, 261, 267 for MHV-JHM and A59, 273 for TGEV and PRCV, 293 on erythrocytes, 299 receptor independant dissemination of MHV, 279 resialylation of erythrocytes, 305 Recombinant vaccines see also Adenovirus expression see also Baculovirus expression see also E.coli, expression in see also Salmonella thyphimurium, expression in see also Vaccinia virus expression Recombination between MHV-JHM DI andmhv-a59,137 DI RNA directed mutagenesis, 149 targeted RNA recombination, 143 Resistance, genetic cell receptors, 267, 273 cellular factor 273 correlation between liver disease and induction of macrophage PeA, 377 differential recovery from MHV Y, 365 regulation of brain endothelial cell adhesion molecules by MHV 4, 389 Ribozyme inhibition of MHV replication, 129 RNA see also Sequence, RNA sense and anti-sense RNA, inhibition of MHV replication, 129 Salmonella Typhimurium, expression in, oftgev S gene fragments, 455 Sequence, RNA BCV non-structural protein NS2, 69 functional domains of ORFl, 75 HCV-229E ORFl, 75 MHV S protein, 177 MHV S protein of avirulent variant, 393 PEDV N protein, 49 protease domain of gene 1,227 TGEV ORF 3a13b, 29 TGEV and PRCV S protein, 35 TGEV S protein, 5' end, 43 TGEV S protein, TFI isolate, 23 3' end of CCV genome, 3 3' end of HCV-OC43 genome, 61 3' end of PEDV genome, 55 Sequence similarity conserved sequences in coronaviruses, 81 of CCV, FECV and APV, 11 of APV variants and FECV, 17 of MHV S protein and mouse FcgR, 183 of HCV 229E, MHV and lbv ORFs la and lb, 75 Sialodacryoadenitis virus, 261 Spike protein S binding to Fc of immunoglobulin, 183 cleavage site, spontaneous aa mutations, 157 site directed mutations, 165, 171 deletions, 43 fusion, see Fusion hemagglutination, 299 induction of DTH reaction, 449 induction of protection, 401, 449 Susceptibility, see Resistance genetic Syncytia, see Fusion T-cell see Cell-mediated immunity 477

5 Temperature-sensitive (ts) mutants genetic map of ts mutants of MHV-A59, 215 N gene of MHV-A59, 143 rescue by homologous recombination, 149 Tissue tropism, see Organ tropism Tol'Oviruses, 255 Transcription intergenic start site, 85, 91 Transmissible gastroenteritis virus TGEV deletion in ORF 3a/3b, 29 genome organisation, 23 mrna isolation, 99 Vaccines see also Recombinant vaccines TGEV,463 Vaccinia virus expression of MHV-JHM S protein, 401 of MHV-JHM S protein mutants, 165, 171 oftgev and CCV S proteins 359 Variants of viruses see also Neutralization-resistant variants FlPV,17 IBV,395 MHV N protein, 143 TGEV,29 Uncoating, virus see Fusion 478