ARC-Onderstepoort Veterinary Research Overview, Ticks, Helminths, Insect Vectors

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1 ARC-Onderstepoort Veterinary Research Overview, Ticks, Helminths, Insect Vectors Deon K. Bakkes Natural Science Collection Facility Launch 18 October Alpine Heath, Drakensberg

2 ARC-OVR TOP STRUCTURE Senior Manager Research: Onderstepoort Veterinary Research Dr. Misheck Mulumba Office Manager Financial Manager Facility Manager Public Relations Officer Research Team Manager: Public Health & Zoonoses Project Coordinator Business Development Coordinator PRO Assistant Research Team Manager: Epidemiology, Parasites & Vectors Quality Coordinator Administrative Officer Research Team Manager: FMD Vaccine Production Human Resources Manager Research Team Manager: Vaccines & Diagnostics Development Occupational Health & Safety Research Team Manager: Diagnostic Services

3 EPIDEMIOLOGY, PARASITES & VECTORS Research Team Manager: EPV Dr. Sikhumbuzo Mbizeni Line Manager: Parasitic Helminths Dr. Kerstin Junker Line Manager: Tick Biology, Taxonomy & Ecology Dr. Ben Mans Line Manager: Ecology & Control of Insect Vectors Dr. Gert Venter Helminth Collection Curator Dr. Kerstin Junker Tick Collection Curator Ms. Heloise Heyne Mr. Deon Bakkes Insect Collection Curator Dr. Karien Labuschagne

4

5 Gertrud Theiler Tick Museum ARC-OVR COLLECTIONS

6 Gertrud Theiler Tick Museum Insect Vectors of Animal Disease

7 Gertrud Theiler Tick Museum Insect Vectors of Animal Disease National Collection of Animal Helminths

8 Gertrud Theiler Tick Museum Insect Vectors of Animal Disease National Collection of Animal Helminths Old data, new methods, better knowledge.

9 Gertrud Theiler Tick Museum Insect Vectors of Animal Disease National Collection of Animal Helminths Old data, new methods, better knowledge. Exploited for taxonomic, ecological and biogeographical research as well as diagnostics.

10 Gertrud Theiler Tick Museum Insect Vectors of Animal Disease National Collection of Animal Helminths Old data, new methods, better knowledge. Exploited for taxonomic, ecological and biogeographical research as well as diagnostics. Priceless intellectual property (IP) value and serves as a vital asset in subject specific training.

11 Gertrud Theiler Tick Museum ARC-OVR COLLECTIONS

12 Gertrud Theiler Tick Museum Ms. Heloise Heyne Mr. Deon Bakkes

13 Gertrud Theiler Tick Museum Ms. Heloise Heyne Mr. Deon Bakkes

14 Gertrud Theiler Tick Museum c tick collections, comprising 375 species from 19 genera of all three Ixodida families. Ms. Heloise Heyne Mr. Deon Bakkes

15 Gertrud Theiler Tick Museum c tick collections, comprising 375 species from 19 genera of all three Ixodida families. Extensive and strategic African collection Ms. Heloise Heyne Mr. Deon Bakkes

16 Gertrud Theiler Tick Museum c tick collections, comprising 375 species from 19 genera of all three Ixodida families. Extensive and strategic African collection 54 type specimens of African tick species Ms. Heloise Heyne Mr. Deon Bakkes

17 Gertrud Theiler Tick Museum c tick collections, comprising 375 species from 19 genera of all three Ixodida families. Extensive and strategic African collection 54 type specimens of African tick species DNA voucher collection (100 species) collaboration with Stellenbosch University Ms. Heloise Heyne Mr. Deon Bakkes

18 Insect Vectors of Animal Disease ARC-OVR COLLECTIONS

19 Insect Vectors of Animal Disease Dr. Karien Labuschagne

20 Insect Vectors of Animal Disease Slide collection: Siphonaptera (fleas) and Phthiraptera (lice): Dr. Karien Labuschagne

21 Insect Vectors of Animal Disease Slide collection: Siphonaptera (fleas) and Phthiraptera (lice): Culicoides (African Horse Sickness) Culicoides species (Afrotropical: 132, World: 78) Dr. Karien Labuschagne

22 Insect Vectors of Animal Disease Slide collection: Siphonaptera (fleas) and Phthiraptera (lice): Culicoides (African Horse Sickness) Culicoides species (Afrotropical: 132, World: 78) Dr. Karien Labuschagne Pinned: 3 Cabinets (general arthropods)

23 Insect Vectors of Animal Disease Slide collection: Siphonaptera (fleas) and Phthiraptera (lice): Culicoides (African Horse Sickness) Culicoides species (Afrotropical: 132, World: 78) Dr. Karien Labuschagne Pinned: 3 Cabinets (general arthropods) Alcohol (light trap samples): Culicoides: > Simulium: 800, 16 species

24 National Collection of Animal Helminths Dr. Kerstin Junker

25 National Collection of Animal Helminths Dr. Kerstin Junker Type collection: c. 246 species Overseas collection: c. 584 accessions Secondary collection: c accessions

26 National Collection of Animal Helminths Dr. Kerstin Junker Type collection: c. 246 species Overseas collection: c. 584 accessions Secondary collection: c accessions Extensive reference collection of parasitic helminths Cestoda (Tapeworms) Trematoda (Flukes) Nematoda (Roundworms) Pentastomida (Tongue worms)

27 National Collection of Animal Helminths Dr. Kerstin Junker Type collection: c. 246 species Overseas collection: c. 584 accessions Secondary collection: c accessions Extensive reference collection of parasitic helminths Cestoda (Tapeworms) Trematoda (Flukes) Nematoda (Roundworms) Pentastomida (Tongue worms) Testimony to helminth diversity in South Africa

28 National Collection of Animal Helminths Dr. Kerstin Junker Type collection: c. 246 species Overseas collection: c. 584 accessions Secondary collection: c accessions Extensive reference collection of parasitic helminths Cestoda (Tapeworms) Trematoda (Flukes) Nematoda (Roundworms) Pentastomida (Tongue worms) Testimony to helminth diversity in South Africa Provides the basis for taxonomic comparisons and for large scale systematic studies

29 National Collection of Animal Helminths Dr. Kerstin Junker Type collection: c. 246 species Overseas collection: c. 584 accessions Secondary collection: c accessions Extensive reference collection of parasitic helminths Cestoda (Tapeworms) Trematoda (Flukes) Nematoda (Roundworms) Pentastomida (Tongue worms) Testimony to helminth diversity in South Africa Provides the basis for taxonomic comparisons and for large scale systematic studies Diagnostic service rendering to the veterinary community

30 Challenges & Risks ARC-OVR COLLECTIONS

31 Challenges & Risks ARC-OVR COLLECTIONS Risk of drying out

32 Challenges & Risks Risk of drying out Alcohol evaporation health risk

33 Challenges & Risks Risk of drying out Alcohol evaporation health risk Fire risk (no protection system)

34 Challenges & Risks Risk of drying out Alcohol evaporation health risk Fire risk (no protection system) Loss of samples (security alarms disconnected)

35 Challenges & Risks Risk of drying out Alcohol evaporation health risk Fire risk (no protection system) Loss of samples (security alarms disconnected) Lack of personnel

36 Challenges & Risks Risk of drying out Alcohol evaporation health risk Fire risk (no protection system) Loss of samples (security alarms disconnected) Lack of personnel Database development

37 Challenges & Risks Risk of drying out Alcohol evaporation health risk Fire risk (no protection system) Loss of samples (security alarms disconnected) Lack of personnel Database development Lack of records in some cases

38 Challenges & Risks Risk of drying out Alcohol evaporation health risk Fire risk (no protection system) Loss of samples (security alarms disconnected) Lack of personnel Database development Lack of records in some cases Procurement delays

39 Challenges & Risks Risk of drying out Alcohol evaporation health risk Fire risk (no protection system) Loss of samples (security alarms disconnected) Lack of personnel Database development Lack of records in some cases Procurement delays Lack of expertise

40 Challenges & Risks Risk of drying out Alcohol evaporation health risk Fire risk (no protection system) Loss of samples (security alarms disconnected) Lack of personnel Database development Lack of records in some cases Procurement delays Lack of expertise Classical skillset appears unattractive to students

41 Challenges & Risks Risk of drying out Alcohol evaporation health risk Fire risk (no protection system) Loss of samples (security alarms disconnected) Lack of personnel Database development Lack of records in some cases Procurement delays Lack of expertise Classical skillset appears unattractive to students Older samples backlog re-labeling re-identification re-organising storage inadequate

42 Challenges & Risks ARC-OVR COLLECTIONS Risk of drying out Alcohol evaporation health risk Fire risk (no protection system) Loss of samples (security alarms disconnected) Lack of personnel Database development Lack of records in some cases Procurement delays Lack of expertise Classical skillset appears unattractive to students Older samples backlog re-labeling re-identification re-organising storage inadequate Yet we persist

43 Current scientific projects

44 Current scientific projects Insect Vectors of Animal Disease National Assets: Surveys, Monitoring (KQS, Paarl, KZN), International collaboration: Reunion, Botswana, Namibia Climate Change: Eastern Cape Completed PhD: The Culicoides Latreille (Diptera: Ceratopogonidae) species of South Africa Species descriptions: Culicoides truuskae Proposals submitted: Rothmansted trap, LEAP-Agri

45 Current scientific projects Insect Vectors of Animal Disease National Assets: Surveys, Monitoring (KQS, Paarl, KZN), International collaboration: Reunion, Botswana, Namibia Climate Change: Eastern Cape Completed PhD: The Culicoides Latreille (Diptera: Ceratopogonidae) species of South Africa Species descriptions: Culicoides truuskae Proposals submitted: Rothmansted trap, LEAP-Agri Gertrud Theiler Tick Museum Book on Ixodid tick species of southern Africa (with UP) Afrotropical Ornithodoros and Ix. pilosus revision - eight new species Rhipicephalus evolution and shape ontogeny (Bakkes PhD, SUN) Tick surveys (NWU, Wageningen University, UL) Rickettsia in ticks screening (NICD) Tick identification services & training Expanding collection

46 Current scientific projects Insect Vectors of Animal Disease National Assets: Surveys, Monitoring (KQS, Paarl, KZN), International collaboration: Reunion, Botswana, Namibia Climate Change: Eastern Cape Completed PhD: The Culicoides Latreille (Diptera: Ceratopogonidae) species of South Africa Species descriptions: Culicoides truuskae Proposals submitted: Rothmansted trap, LEAP-Agri Gertrud Theiler Tick Museum Book on Ixodid tick species of southern Africa (with UP) Afrotropical Ornithodoros and Ix. pilosus revision - eight new species Rhipicephalus evolution and shape ontogeny (Bakkes PhD, SUN) Tick surveys (NWU, Wageningen University, UL) Rickettsia in ticks screening (NICD) Tick identification services & training Expanding collection National Collection of Animal Helminths Diversity and patterns of helminths in small mammals Pentastomids and gastrointestinal helminths in Nile Crocodiles Helminth diversity in warthogs, host sex-age influence on infection Taxonomy of the gastric nematode genus Cylicospiura in carnivores Expanding collection Sampling for future molecular work Student training

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