Parasympathetic Nervous System Part I

Transcription

1 Parasympathetic Nervous System Part I Edward JN Ishac, Ph.D. Professor Smith Building, Room 742 eishac@vcu.edu Department of Pharmacology and Toxicology Medical College of Virginia Campus of Virginia Commonwealth University Richmond, Virginia, USA Autonomic Nervous System 1

2 ANS Diagram Key Points Division Anatomical Usually dual innervation Usually antagonistic Usually one dominates Usually some ANS tone Neurons of the ANS Key Points Preganglionic fibers mylinated Postganglionic fibers non mylinated SNS pre : post 1:20 PNS pre : post 1:1 (exception 1:10,000 Auerbachs plexus) Key role of Ach Motor fiber not part of ANS 2

3 Cholinoceptors Muscarinic M 1 - M 2 - M 3 - Ganglia cells Cardiac muscle Sweat glands Nicotinic M 4 /M 5 N N - N M - Ganglia cells Neuromuscular junction Neurons of the ANS 3

4 Cholinergic Neurotransmission Rate limiting step Uptake of choline into nerve terminal Synthesis Choline Acetyltransferase Termination Enzymatic by acetylcholinesterase (AchE) Cholinergic Receptors Na+ - in K+ - out Muscarinic (7 transmembrane) -M 1 -autonomic ganglia, CNS -M 2 -heart -M 3 -smooth muscle, glands -M 4, M 5 -M 135 PLC, M 24 A/C - G-protein coupled Nicotinic (ion channel) - pentamer, 5 subunits -N N or N 1 -ganglia, adrenal medulla (α 2 β 3, α 3 β 2 ) -N M or N 2 -skeletal muscle (infant α2βδε, adult α2βδγ ) - α subunit, Ach binding (2) 4

5 True Acetylcholinesterase (AchE) (Other: Pseudocholinesterase, circulating, plasma, butylcholinesterase) Nerves NMJ Circul n AchE Yes Yes Little BuChE Little Little Yes Quaternary group Acyl carbon AchE: 300,000 Ach / enzyme / min (0.15 msec/cycle) Muscarinic effects on organ systems Heart (M2) - HR, contractility, conduction velocity Vasculature (not innervated) - vasodilation: nitric oxide (NO) Other smooth muscle -Eye:pinpoint pupil (miosis), focus for near vision - GI-tract: tone to intestine, bladder, tone to sphincters - Lung: contract bronchial SM. resistance, secretions - Exocrine glands: sweating (cholinergic sympathetic) salivation, gastric acid secretion (M1) 5

6 Cholinergic Stimulants Ach Pilocarpine Muscarine Ach Nicotine Physostigmine Neostigmine Edrophonium Malathion DFP Nerve gas Muscarinic receptor agonists Choline esters - ACH (muscarinic & nicotinic action) - bethanechol (oral or sc, never iv or im cardiac arrest) - methacholine (not common) - carbachol (direct/indirect; muscarinic & nicotinic) Alkaloids: - muscarine (mushrooms) - pilocarpine (DOC, used in glaucoma emergency) - oxotremorine (synthetic) CNS action (basal ganglia) Uses: - glaucoma treatment - ophthalmic (Ach, brief miosis) - diagnostic for belladonna poisoning (methacholine) - urinary retention (bethanechol) - reverse GIT depression (bethanechol) 6

7 Wild Mushrooms - Amanita 10,000 cases per year Muscarine poisoning 5,000 mushroom species 100 bad, 10 deadly Adverse Reactions - Cholinergics Adverse reactions: (SLUDE) - Salivation - Lacrimation - Urination - Diarrhea - Emesis (vomiting) - cardiac slowing (arrest, esp. bethanechol) - nausea, cramps - bronchoconstriction, can precipitate asthma - involuntary defecation, urination - tremor, CNS induced convulsions Also: DUMBELS, SLUGBAM and MTWtHF (nicotinic excess) 7

8 Symptoms of Parasympathetic Toxicity SLUDGE S-Salivation L - Lacrimation U - Urination D - Diarrhea G - Gastric upset E-Emesis DUMBBELS D - Diarrhea U - Urination M - Miosis/muscle weakness B - Bronchorrea ( mucus) B - Bradycardia E-Emesis L - Lacrimation S - Salivation/sweating Adverse Reactions Cholinergics cont.. Slugbam (muscarinic excess): - Salivation, seizure - Lacrimation - Urination - GI distress: diarrhea, vomiting - Bronchoconstriction - Abominal cramps - Miosis MTWThF (nicotinic excess): - Mydriasis - Tachycardia - Weakness (muscle paralysis) - Th Hyperthermia - Fasciculations 8

9 Nicotinic receptor agonists Ganglionic stimulants Clinically not important Acetylcholine (natural transmitter) DMPP (experimental) Nicotine (alkaloid, tobacco) Lobeline (tobacco) Indirectly-Acting Parasympathomimetics Interact with acetylcholinesterase True and/or pseudocholinesterase (serum) Two sites: - anionic site that binds the quaternary amine and positions the Ach molecule - esteratic site which attacks the acyl carbon Inhibitors of cholinesterase: - Reversible inhibitors (eg. physostigmine) - Irreversible inhibitors (eg. organophosphates) 9

10 Reversible inhibitors Quarternary ammonium compounds - Edrophonium (synthetic, water stable, 5-10 min) Tensilon test Myasthenia gravis - Ambenonium (synthetic, 4-8 hr) Carbamates - Physostigmine (0.5-2 hr) (tertiary amine, well absorbed, cns activity, can give topically) - Neostigmine (0.5-2 hr) (quaternary amine, no cns activity, synthetic, some direct action) Myasthenia gravis Autoimmune disease 1:10,000 (250,000 USA) antibodies to NMJ nicotinic receptors leads to degradation simplified synaptic folds normal nerve terminal and transmitter wider synaptic junction Diagnosis: Edrophonium (Tensilon, short acting) is used for diagnosis and determination of maintenance dose Treatment: Neostigmine has direct (stimulates receptor) and indirect actions (inhibition of AchE). No cns activity. 10

11 Acetylcholinesterase and Reversible inhibitors ACH Neostigmine Ach very fast 0.15msec Neostigmine undergoes metabolism hr Enzyme becomes operational again Irreversible inhibitors Organophosphates (highly lipid soluble, >50,000 compounds) - Diisopropyl-fluorophosphate (DFP) - Echothiophate (low lipid solubility, no CNS) - Sarin, Suman, Vx (nerve gases) - Malathion, Parathion (more toxic) Prodrugs, inactive, converted to active compounds in body (S O) pesticides, very lipid soluble 11

12 Acetylcholinesterase & Irreversible Inhibition DFP, Isoflurophate R1 P O 2-PAM Pralidoxime No cns action R2 X DFP Aging min Nerve gas secs / min Malathion 4 6 hr US Military 2-PAM / Atropine Injector 2.5 mg Atropine, 600mg 2-PAM 12

13 Clinical use: Acetylcholinesterase Inhibitors Eye: miosis (sphincter contraction), accommodation block (ciliary muscle contraction) Use: Glaucoma (wide-angle or secondary glaucoma) Physostigmine or echothiophate (long acting) GI tract: motility in paralytic ileus (post-op) or atony of urinary bladder. Neostigmine (bethanechol better) Neuromuscular junction: - Neostigmine in Myasthenia gravis - Edrophonium as diagnostic Myasthenia gravis - Reverse NMJ block after surgery, Neostigmine Reverse toxicity by anticholinergic agents: - ie. atropine, tricyclic antidepressants (high doses) - Physostigmine is preferred (CNS action) Actions on the Eye Glaucoma treatment 1. α-agonist Outflow 2. M-Agonists Outflow 3. β-blocker Secretion 4. α2-agonist Secretion 5. PGs: Outflow 6. Carbonic acid inhibitors Secretion 13

14 Acetylcholinesterase Inhibitors Alcohols Edrophonium (Tensilon) Carbamates and related agents Neostigmine (Prostigmine, ) Pyridostigmine (Mestinon) Physostigmine (Eserine) Ambenonium (Mytelase) Demecarium (Humorsol) Organophosphates Echothiophate, DFP, Malathion, (Phospholine), nerve gases etc.) Myasthenia gravis Glaucoma Myasthenia gravis Glaucoma Glaucoma Uses Myasthenia gravis, ileus, arrhythmias Myasthenia gravis, ileus, NMJ reverse 5-15 minutes 1/2-2 hours 3-6 hours 1/2-2 hours 4-8 hours 4-6 hours 100 hours Duration of Action Neurons of the ANS 14

15 Toxicity & Treatment of AchE Inhibitors Adverse reactions: (SLUDE) - Salivation (muscarinic) - Lacrimation (muscarinic) - Urination (muscarinic) - Diarrhea (muscarinic) - Emesis (vomiting) (muscarinic) - cardiac slowing (muscarinic) - Hypertension / hypotension (nicotinic) - NMJ paralysis (nicotinic) - cramps (muscarinic) - bronchoconstriction (muscarinic) - tremor, nausea, CNS induced convulsions Treatment: Muscarinic antagonist ie. Atropine AchE reactivator (Pralidoxime, 2-PAM) Mechanical respiration NMJ Blocking Agents 15

16 Symptoms of Parasympathetic Toxicity SLUDGE S-Salivation L - Lacrimation U - Urination D - Diarrhea G - Gastric upset E-Emesis DUMBBELS D - Diarrhea U - Urination M - Miosis/muscle weakness B - Bronchorrea ( mucus) B - Bradycardia E-Emesis L - Lacrimation S - Salivation/sweating Adverse Reactions Cholinergics cont.. Slugbam (muscarinic excess): - Salivation, seizure - Lacrimation - Urination - GI distress: diarrhea, vomiting - Bronchoconstriction - Abominal cramps - Miosis MTWThF (nicotinic excess): - Mydriasis - Tachycardia - Weakness (muscle paralysis) - Th Hyperthermia - Fasciculations 16