Effect of Ca 2 + /CaM Messenger on Drought Resistance of Phyllostachys edulis

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1 http / /xuebao. jxau. edu. cn Acta Agriculturae Universitatis Jiangxiensis E - mail ndxb7775@ sina. com Ca 2 + /CaM * EGTA LaCl 3 heparin CPZ CaCl 2 CaCl 2 MDA EGTA LaCl 3 heparin CPZ SOD CAT LaCl 3 heparin MDA heparin CPZ POD Ca 2 + /CaM CaCl 2 S A Effect of Ca 2 + /CaM Messenger on Drought Resistance of Phyllostachys edulis JIANG Qin YING Ye-qing * XIE Nan-nan FANG Wei The Nurturing Station for the State Key Laboratory of Subtropical Silviculture Zhejiang Agriculture and Forest University Lin an China Abstract The effect of calcium messenger on main drought resistance physiological indexes of Phyllostachys edulis was studied by investigating the activities of antioxidant enzymes the content of proline MDA hydrogen peroxide and soluble protein in Phyllostachys edulis under water stress after being blocked calcium messenger transduction by EGTA LaCl 3 and heparin CPZ and CaCl 2. Compared with the control CaCl 2 treatment increased SOD POD and CAT enzyme activity and soluble protein content decreased MDA proline and hydrogen peroxide content. Compared with the control EGTA LaCl 3 and heparin and CPZ could increase the hydrogen peroxide content decrease SOD and CAT enzyme activity and proline and soluble protein content. However LaCl 3 and heparin led to little changes of MDA content heparin and CPZ had no obviously effect on POD enzyme activity. The results suggested that Ca 2 + /CaM messenger might control drought resistance of Phyllostachys edulis by affecting activities of antioxidant enzymes and contents of proline and soluble protein through different transduction paths to affect them. Key words Phyllostachys edulis calcium messenger drought stress calcium signal blocking agent CaCl C E - mail jq629@ sina. com * E - mail yeqing@ zafu. edu. cn

2 CaM Ca 2 + Ca Ca 2 + EGTA LaCl 3 heparin CPZ CaCl Phyllostachys edulis % 1 6 EGTA LaCl 3 heparin CPZ CaCl mmol / L 40% 5 d % 1. 3 MDA TBA SOD NBT NBT 50% POD CAT H 2 O 2 Mukherjee Choudhari Excel SPSS17. 0 P < P < MDA MDA 15 MDA 1 F = P = F = P = MDA EGTA CPZ heparin MDA CaCl 2 MDA LaCl 3 EGTA CPZ heparin P < EGTA CPZ heparin MDA MDA MDA 2. 2 SOD POD CAT SOD POD CAT

3 4 Ca 2 + /CaM SOD Fig. 1 Effect of different treatments on MDA content in leaves of Phyllostachys edulis 2 MDA Fig. 2 Effect of different treatments on SOD activity in leaves of Phyllostachys edulis SOD 2 SOD F = P = SOD heparin CPZ LaCl 3 EGTA SOD CaCl 2 SOD heparin LaCl 3 EGTA CPZ EGTA P < heparin CPZ LaCl 3 EGTA SOD CAT 3 CAT F = P = < LaCl 3 heparin EGTA CPZ CaCl 2 CAT CaCl 2 CAT LaCl 3 heparin EGTA CAT P < CaCl 2 CAT CAT 3 CAT Fig. 3 Effect of different treatments on CAT activity in leaves of Phyllostachys edulis 4 POD Fig. 4 Effect of different treatments on POD activity in leaves of Phyllostachys edulis POD 4 POD F = P = < EGTA heparin LaCl 3 POD CPZ POD CaCl 2 POD 1 10 POD 15 25

4 Fig. 5 Effect of different treatments on proline content in leaves of Phyllostachys edulis Fig. 6 Effect of different treatments on soluble protein content in leaves of Phyllostachys edulis 2. 3 POD 5 CaCl 2 F = P < heparin CPZ P < P < EGTA CPZ heparin LaCl 3 CaCl EGTA LaCl 3 heparin CPZ P < CaCl Fig. 7 Effect of different treatments on hydrogen peroxide EGTA LaCl 3 heparin in leaves of Phyllostachys edulis CPZ LaCl 3 EGTA heparin CPZ F = P = < CaCl 2 LaCl 3 EGTA heparin CPZ CAT 3 SOD POD CAT

5 4 Ca 2 + /CaM SOD CAT POD CAT SOD POD CAT Ca 2 + SOD POD 19 SOD CAT 20 CaCl 2 SOD POD CAT SOD POD CAT MDA 21 Ca 2 + POD CaCl 2 POD heparin CPZ POD CAT 3 9 CaM POD CPZ POD CaM SOD TFP CaM SOD 7 CPZ SOD EGTA Ca 2 + heparin LaCl 3 CPZ 3 Ca 2 + Ca EGTA CPZ heparin LaCl 3 CaCl 2 24 LaCl 3 CPZ 24 MDA MDA EGTA LaCl 3 heparin CPZ CaCl 2 EGTA Ca 2 + LaCl 3 heparin Ca ku EGTA Ca Ca 2 + verapamil ku CaCl 2 1 Knight H Trewaras A J Knight M R. Calcium signaling in Arabidopsis thaliana responding to drought and salinity J. Plant

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