NDO NSP. YI Zhong-hua ZHU Nian-hua LI Guan-hong SONG Xiao-zhen PAN Ke QU Ming-ren

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1 http / /xuebao. jxau. edu. cn Acta Agriculturae Universitatis Jiangxiensis E - mail ndxb7775@ sina. com NDO NSP NDO NSP NSP NSP NSP NSP NSP NSP NSP NSP NSP S A Comparative Evaluation of the in Vitro Fermentation Characteristics of Selected Non-digestible Oligosaccharides and Non-starch Polysaccharides Using Caecal Microbial Inoculum from Broilers YI Zhong-hua ZHU Nian-hua LI Guan-hong SONG Xiao-zhen PAN Ke QU Ming-ren College of Animal Science and Technology JAU Nanchang China Abstract An in vitro experiment in comparison of fermentation characteristics of selected non - digestible oligosaccharides NDO and non - starch polysaccharides NSP was conducted using caecal microbial inoculum from broilers fed an antibiotics - free diet. The results showed that the total production of SCFA from the fermentation of NDO was higher than that of NSP. The fermentability ranked in the order of Raffinose > Stachyose > Fructooligosaccharides FOS > mannanoligosaccharides MOS in NDO and ranked in the order of Wheat NSP > Soybean meal NSP > Corn NSP > Wheat bran NSP > Cellulose in NSP. In addition the molar percentage of acetate from the fermentation of NSP was higher whereas the percentage of propionate from that of NDO was higher. Compared with insoluble NSP the fermentation characteristics of soluble NSP was closer to that of NDO. Key words non-digestible oligosaccharides non-starch polysaccharides broilers in vitro fermentation short-chain fatty acid GJJ E - mail yizhcn@ 126. com

2 Non - digestible oligosaccharide NDO Non - starch polysaccharide NSP NSP AA AA % 4. 69% 2. 18% % 7. 03% 3. 29% 1. 83% 1. 13% % % 7. 06% > 99. 0% > 28% Choct Annison 6 NSP Sunvold g g 250 ml 30 ml min ml CO ml CO h 4 ml SCFA ph Lee 8 4 NSP TDF SDF IDF 9 4 NSP DM CP Carre 10

3 2 NDO NSP Excel SPSS One - Way ANOVY Duncan ± X ± S P < NSP NSP % % % 4 NSP NSP NSP % NSP NSP NSP NSP NSP Tab. 1 1 % Composition of dietary fiber and the alkali extracted NSP of the feedstuffs % Air dry basis Item Corn Wheat Soybean meal Wheat bran Composition of the feedstuffs Dry matter Crude fiber Total dietary fiber Insoluble dietary fiber Soluble dietary fiber / SDF /TDF NSP Alkali extracted NSP NSP Composition of the alkali extracted NSP Dry matter Crude protein NSP Crude fiber Total dietary fiber Insoluble dietary fiber Soluble dietary fiber / SDF /IDF h NDO SCFA NSP NSP SCFA NDO NDO SCFA NSP 24 h NDO 6 h SCFA P < SCFA P < h SCFA 6 h 12 h SCFA 50% 24 h SCFA % % h SCFA % % % 4 NDO

4 Tab h Short - chain fatty acid production after onset of 6 12 and 24 hours fermentation of various NDO or NSP by caecal microflora in broilers mmol /g DM Acetate mmol /g DM Propionate Stachyose ± e ± d ± e ± d ± c ± c Raffinose ± f ± e ± f ± e ± d ± c FOS ± d ± d ± e ± c ± d ± c MOS ± c ± bc ± d ± b ± a ± b NSP Corn NSP ± ab ± bc ± bc ± a ± a ± a NSP Wheat NSP ± bc ± c ± cd ± ab ± ab ± a NSP Wheat bran NSP ± ab ± ab ± ab ± a ± a ± a NSP Soybean meal NSP ± ab ± bc ± bc ± a ± a ± a Cellulose ± a ± a ± a ± a ± a mmol /g DM Butyrate SCFA mmol /g DM TSCFA Stachyose ± b ± c ± c ± e ± d ± e Raffinose ± c ± d ± d ± f ± e ± f FOS ± a ± bc ± c ± d ± d ± e MOS ± a ± ab ± b ± c ± bc ± d NSP Corn NSP ± a ± a ± a ± ab ± b ± bc NSP Wheat NSP ± a ± c ± ab ± bc ± c ± cd NSP Wheat bran NSP ± a ± a ± a ± a ± ab ± ab NSP Soybean meal NSP ± a ± ab ± ab ± ab ± bc ± bc Cellulose ± a ± a ± a P < P > 0. Means in the same column with different superscripts are significantly different P < Tab h Molar percentage of short - chain fatty acid after onset of 6 12 and 24 hours fermentation of various NDO or NSP by caecal microflora in broilers mol /100 mol Acetate mol /100 mol Propionate mol /100 mol Butyrate Stachyose ± b ± c ± ab ± a ± c ± d ± ± ab ± de Raffinose ± a ± a ± ab ± c ± d ± d ± ± d ± e FOS ± b ± a ± a ± b ± e ± d ± ± a ± de MOS ± b ± b ± b ± ab ± cd ± d ± ± ab ± bc NSP Corn NSP ± b ± ef ± d ± ab ± ab ± bc ± ± b ± a NSP Wheat NSP ± b ± d ± c ± ab ± b ± c ± ± cd ± c NSP Soybean meal NSP ± b ± f ± d ± a ± a ± ab ± ± b ± ab NSP Wheat bran NSP ± b ± de ± c ± ab ± ab ± bc ± ± bc ± cd Cellulose ± c ± g ± e ± a ± a P < P > 0. Means in the same column with different superscripts are significantly different P < NSP NSP SCFA NDO NSP h SCFA NSP % % % NSP NSP NSP h SCFA NSP % % % % % % h SCFA NSP % % 5 NSP NSP NSP NSP NSP 3

5 2 NDO NSP VFA 3 NSP NSP NSP NSP VFA 4 1 α α ~ 3 β NSP NSP NSP NSP NSP NSP NSP NSP NSP NSP NSP NSP NSP 11 NSP NSP NSP % Macfarlane 12 SCFA SC- FA NSP NSP NSP NSP NSP NSP NSP NSP NSP NSP SCFA NSP 4 5 NSP NSP NSP NSP NSP NSP NSP NSP NSP 1 Englyst H N Kingman S M Cumming J H. Classification and measurement of nutritionally important starch fractions J. Eur J Clin Nutr Flamm G Glinsmann W Kritchevsky D et al. Inulin and oligofructose as diatary fiber A review of the evidence J. Crit Rev in Food Sci Nutr J

6 Bedford M. Removal of antibiotic growth promoters from poultry diets implications and strategies to minimize subsequent problems J. World s Poult Sci J Verstegen M W Williams B A. Alternatives to the use of antibiotics as growth promoters for monogastric animals J. Anim Biotechnol Choct M Annison G. Anti - nutritive effect of wheat pentosans in broiler chickens roles of viscosity and gut microflora J. Br Poult Sci Sunvold G D Hhussein H S Jr Fahey G C et al. Reinha. In vitro fermentation of cellulose beet pulp citrus pulp and citrus pectin using fecal inoculum from cats dogs horses humans and pigs and ruminal fluid from cattle J. J Anim Sci Lee S C Porsky L De Vries J W. Determination of total soluble and insoluble dietary fiber in foods - enzymatic - gravimentric method MES - TRIS buffer collaborative study J. J AOAC Int M Carre B Gomez J Chagneau A M. Contribution of oligosaccharide and polysaccharide digestion and excreta losses of lactic acid and short chain fatty acids to dietary metabolizable energy values in broiler chickens and adult cockerels J. Br Poult Sci Schneeman B O. Soluble V S. insoluble fiber - different physiological responses J. Food Technol Macfarlane G T Allison C Gibson S A W et al. Contribution of the microflora to proteolysis in the human large intestine J. J App Bacteriology 櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗櫗 J J J Bagnall C R. Species identification among pollen grains of Abies Picea and Pinus in the Rocky Mountain A scanning electron microscope study J. Rev Palaeobotany Palynology Erdtman G. Handbook of palynology - an introduction to the study of pollen grains and spores J. Copenhagen Munksgaard M J J J J J J J J J J M M J Camellia japonica L. J J Donald E Stone. Pollen size in hickories Carya J. National Science Foundation Grant No. G