Alpha Fetoprotein AFP AFP AFP AFP AFP
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1 Alpha Fetoprotein AFP AFP SPSSAFP AFP AFP r = AFP
2 Alpha Fetoprotein(AFP) KDa HSA(HumanSerumAlbumin)L-AlbuminD AFP AFP mg/ml AFP AFP AFP AFP AFP AFP AFP () AFP AFP AFP AFP AFP ( AFP AFP AFP AFP AFP AFP AFP AFP AFP AFP AFP CanAgAFP Enzyme Immune Assay) EIA (Direct Sandwich AFPtechnique) Stat fax (Absorbance) SPSS-13Diazotization ANOVA t P
3 mg/dl mg/dl AFP l g/ g/l g/l AFP AFP AFP AFP r AFP AFP AFP l g/ AFP AFP Pvalue AB A B O AFP dl mg/ AFP l g/
4 AFP P Lee AFP AFP AFP P AFP AFP P mg/ dl AFP Ikonen P AFP FP g/l g/l AFP AFP AFP CarlloP Goraya AFP AFP AFP AFP g/l g/l g/l
5 References: 1. American Academy of Pediatrics, Subcommittee on Neonatal Hyperbilirubinemia. Neonatal jaundice and kernicterus. Pediatrics 2001; 108: American Academy of Pediatrics, Provisional Committee for Quality Improvement and Subcommittee on Hyperbilirubinemia. Practice parameter: management of hyperbilirubinemia in the healthy term newborn. Pediatrics 1994; 94: Shibahara S, Kitamuro T, Takahashi K. Heme degradation and human disease: Diversity is the soul of life. Antioxid Redox Signal 2002; 4(4): Bergstrand CG, Czar B. Demonstration of a new protein fraction in serum from the human fetus. ScandJClinLabInvest1956;8(2): Ruoslahti E, Sepp-Fetoprotein in normal human serum, Nature 1972; 235: Morinaga T, Sakai M, Wegmann G, Tamaoki T. Primary structure of human -fetoprotein and mrna. Proc Natl Acad Sci 1983; 80: Deutsch HF. Chemistry and biology of fetoprotein. Adv Cancer Res 1991; 56: Sepp, Ruoslahti E. Alpha fetoprotein: Physiology and pathology during pregnancy and application to antenatal diagnosis. J Perinat Med 1973; 1(2): Brock DJ, Scrimgeour JB, Nelson MM. Amniotic fluid alpha fetoprotein measurements in the early prenatal diagnosis of central nervous system disorders. Clin Genet 1975; 7: Seppfetoprotein. Ann NY Acad Sci 1975; 22: 259, Ruoslahti E, Sepp. Alpha-Fetoprotein in cancer and fetal development. Adv Cancer Res 1979; 29: Abelev GI. -Fetoprotein in angiogenesis and its association with malignant tumors. Adv Cancer Res 1971; 14: Okuda K, Kotoda K, Obata H, Hayashi N, Hisamitsu T. Clinical observations during a relatively early stage of hepatocellular carcinoma, with special reference to serum alphafetoprotein levels. Gastroenterology 1975; 69(1): Yoshiki T, Itoh T, Shirt T, Noro T, Tomino Y, HamaJima TI. Primary intracranial yolk sac tumor immuno- fluorescent demonstration of alphafetoprotein synthesis, Cancer 1976; 37: DeMeesC,LaesJF,BakkerJ,SmitzJ,HennuyB, Van Vooren P, etal. Alpha-fetoprotein controls female fertility and prenatal development of the gonadotropin-releasing hormone pathway through an antiestrogenic action. Mol Cell Biol 2006; 26(5): Soloff MS, Swartz SK, Pearlmutter AF, Kithier K. Binding of 17beta-estradiol by variants of alphafetoprotein in rat amniotic fluid. Biochim Biophys Acta 1976; 427(2): Uriel J, De Nechaud B, Dupiers M. Estrogenbinding properties of rat, mouse and man fetospecific serum proteins. Demonstration by immuno-autoradiographic methods. Biochem Biophys Res Commun 1972; 46(3): NishiS,MatsueH,YoshidaH,YamaotoR,Sakai M. Localization of the estrogen-binding site of alpha-fetoprotein in the chimeric human-rat proteins. Proc Natl Acad Sci USA 1991; 88 (8): Savu L, Benassayag C, Vallette G, Christeff N, Nunez E. Mouse alpha 1-fetoprotein and albumin. A comparison of their binding properties with estrogen and fatty acid ligands. J Biol Chem 1981; 256(18): Uriel J, BouillonD,AusselC,DupiersM.Alphafetoprotein: the major high-affinity estrogen binder in rat uterine cytosols. Proc Natl Acad Sci USA 1976; 73(5): Mizejewski GJ. New insights into AFP structure and function: potential biomedical applications, In: Mizejewski GJ and Porter IH (eds), Alpha- Fetoprotein and Congenital Disorders. Orlando, Academic Press, PP: Kohler KD. The pre- and post natal influence of hormones and neurotransmitters on sexual differentiation of the mammalian hypothalamus. IntRevCytol1991;131: Toran-Allerand CD. On the genesis of sexual differentiation of the general nervous system: morphogenetic consequences of steroidal exposure and possible role of alpha-fetoprotein. Prog Brain Res 1984; 61: MccLusky NJ, Naftolin F. Sexual differentiation of the central nervous system. Science 1981; 211: Gillespie JR, Uversky VN. Structure and function of alpha-fetoprotein: a biophysical overview Biochim.BiophysActa2000;1480: Aoyagi Y, Ikenaka T, Ichida F. Copper(II)-binding ability of human alpha-fetoprotein, Cancer Res 1978; 38: Torres JM, Laborda J, Naval J, Darracq N,Calvo M, Mishal Z, etal. Expression of alpha-fetoprotein receptors by human T-lymphocytes during blastic transformation. Mol Immunol 1989; 26(9): Belanger L, Waite WI, Daguillard F, Larochelle J, DufourD.TheeffectofAFPontheinvitrolym phocyte response. In: Masseyeff R, (ed.) Alpha- Fetoprotein, Institut National De La Sante LaRechercheMe1974:
6 29. Tomasi TB Jr. Structure and function of alphafetoprotein. Annu Rev Med 1977; 28: Bellini C, Bonacci W, Paridu E, Serra G. Serum alpha-fetoprotein in newborns. Clin Chem 1998; 44(12): Goraya SS, Smythe PJ, Walker V. Plasma alpha - feto protein concentration in preterm neonates, Ann Clin Biochem 1985; 22: Obiekwe BC, Malek N, Kitau MJ, Chard T. Maternal and fetal alphafetoprotein (AFP) level at term, Acta Obstet Gynecol Scand 1985; 64: Dierks Tan JS, Zehfuss I, Taubert HD. The significance of alpha-feto-protein (AFP) and human chorionic gonodotrophi (HCG) during the first half of pregnancy. 1982; 42(1): Ikonen RS, Lindgren J, Niemi E, Sorto AE, Sepp -fetoproteinlevelin neonatal hyper bilirubinemia. Acta Paediatr Scand 1980; 69(1): Riskin A, David M, Peskin B, Tamir A, Vafsi O, Leibovitz Z, etal. The role of umbilical cord alpha fetoprotein as a screening tool for neonatal hyperbilirubinemia. Am J Perinatol 2004; 21(2): National committee for clinical laboratory standards. National Evaluation protocols for interference testing. Evaluation Protocol 1986; 7(6): National committee for clinical laboratory standards. Evaluation of precision performance of clinical chemistry devices, approved guideline, 2 nd ed. Wayne 2001; EP5-A. 38.Lee PI, Chang MH, Chen DS, Lee CY. Serum alpha-fetoprotein levels in normal infants: a reappraisal of regression analysis and sex difference. J Pediatr Gastroenterol Nutr 1989; 8(1):
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