LEUCOGNOST. Diagnostic-systems for the detection of leukaemias and myeloproliferative diseases. Focussing your hematology targets.

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1 LEUCOGNOST Diagnostic-systems for the detection of leukaemias and myeloproliferative diseases Focussing your hematology targets.

2 LEUCOGNOST LEUCOGNOST ALPA Cytochemical staining for the differentiation of leukaemia and myelodysplastic syndromes In the clinical routine examination are the main indications for accomplishment of cytochemical examinations pathogenic unclear leukocytosis and their delimitation of chronically myeloproliferative diseases, the appearance by atypical cell types, which can not become classified by morphological criteria alone as well as above all the classification of acute leukaemia and myelodysplastic syndromes. At malignant lymphomas cytochemical reactions completes the immunological and morphological findings. At diseases of the erythropoiesis are proof methods of iron and carbohydrate important supplements for morphology. Cytochemical examination methods file with it in that today available arsenal of morphological, immunological, cytogenetic and molecular investigation methods. They form together with the panoptical staining methods the basis of the investigation course, which is to complete according to the requirements. Alkaline leukocytephosphatase-reaction Positive reaction in leukocytes (Magnification: 280 x0.25) Basic performance of LEUCOGNOST Thin air dried smears from blood or bone marrow not more than three days old are necessary. It should be used if possible only native blood samples because the reaction decreased due of the use of anticoagulation agents like EDTA and citrate. Fixation of the air dried smears in LEUCOGNOST mixture of fixative Stain the slide freshly prepared staining solution* Counterstain with Mayer s hemalum Wash with tap running water Air dry Cover with Aquatex Detection of the activity of alkaline phosphatase in leukocytes to the delimitation of the chronic myelogenous leukaemia to myeloid reactions and other myeloproliferative disorders. Performance HEMATOGNOST Fe Fixation of the air dried smears in methanol Air dry Stain the slide freshly prepared staining solution* Counterstain with Nuclear fast red solution Air dry Cover with Entellan new * Preparation the staining solution and staining time see package insert of the respective staining kits.

3 LEUCOGNOST EST LEUCOGNOST NASDCL LEUCOGNOST PAS a-naphthyl-acetateesterase-reaction Leukocyte with positive reaction Detection of the periodic-acid- Schiff-reaction in leukocytes to the delimitation of lymphatic cells and to the diagnosis of erythroleukaemia. Naphthol-AS-Dchloroacetate-reaction Positive reaction with cells of the myeloic line in the bone marrow (Magnification: 1000 x 0.32) Periodic-Acid-Schiff-reaction Positive reaction with cells of the myeloic line in the bone marrow (Magnification: 1000 x 0.32) Detection of a-naphthyl-acetate esterase in leukocytes to the delimitation acute myelomonocytic leukaemia of acute myelogenous leukaemia. Detection of the chloroacetateesterase (specific esterase) in leukocytes to the delimitation acute myelomonocytic leukaemia (M4Eo) and hypergranular promyelocytic leukaemia (M3) from other leukaemia. Ordering information Product Merck Cat.No. Package size LEUCOGNOST ALPA Staining batches LEUCOGNOST EST Staining batches LEUCOGNOST NASDCL Staining batches LEUCOGNOST PAS Staining batches LEUCOGNOST POX Staining batches LEUCOGNOST SP Staining batches HEMATOGNOST Fe Staining batches

4 LEUCOGNOST POX LEUCOGNOST SP HEMATOGNOST Fe Peroxidase-reaction Acute myeloblastic leukaemia Acid phosphatase-reaction Acute T-lymphoblastic leukaemia in the bone marrow Iron staining (Berliner Blue-reaction) Sideroblasts with positive reaction Detection of the myeloperoxidase in leukocytes to the delimitation acute myelogenous of acute lymphoblastic leukaemia. Detection the acid phosphatase in leukocytes to the delimitation T-lymphoblastic cells and for diagnosis of hairy-cell leukaemia with tartrate-resistant acid phosphatase. Detection the free iron ions in somatic cells. Display of sideroblasts and siderocytes and detection of storage iron in macrophages and endothelial cells. Additional reagents Product Merck Cat.No. Package size LEUCOGNOST basic kit Mayers Hemalum 500 ml, Schiffs reagent 500 ml, LEUCOGNOST fixing mixture 2x500 ml, Aquatex 20 ml, Acetone GR ACS, ISO 20 ml, Ethanol GR ACS, ISO 500 ml LEUCOGNOST fixing mixture ml Aquatex (aqueous mounting medium) ml Entellan (non-aqueous mounting medium) ml Entellan new (non-aqueous mounting medium) ml, 500 ml Mayers Hemalum ml, 2.5 l Schiffs reagent ml Immersion oil acc. DIN ISO ml Acetone GR for analysis ACS, ISO l Ethanol GR for analysis ACS, ISO l Methanol GR for analysis ACS, ISO l, 2.5 l

5 Advantages of the LEUCOGNOST -System All stainings at room temperature Easy to use Fast and safe processes All reagents from one source The same careful fixation for all stainings First-class and reproducible staining pictures According to the standard literature Advantages of HEMATOGNOST Fe Simply operation of the staining kit Quick and sure results in less than 30 minutes First-class, reproducible staining picture According to the standard literature High-contrast display by nuclear fast red counterstain

6 Further information: Merck KGaA Darmstadt, Germany Phone +49 (0) 6151/72-0 Fax +49 (0) 6151/ W /03