Pre-conception maternal erythrocyte saturated to unsaturated fatty acid ratio predicts pregnancy after natural cycle frozen embryo transfer

Transcription

1 University of Wollongong Research Online Faculty of Science, Medicine and Health - Papers Faculty of Science, Medicine and Health 2018 Pre-conception maternal erythrocyte saturated to unsaturated fatty acid ratio predicts pregnancy after natural cycle frozen embryo transfer Christopher C. Onyiaodike University of Glasgow Heather Murray University of Glasgow Ruiqi Zhang University of Glasgow Barbara J. Meyer University of Wollongong, bmeyer@uow.edu.au Fiona Jordan University of Glasgow See next page for additional authors Publication Details Onyiaodike, C. C., Murray, H. M., Zhang, R., Meyer, B. J., Jordan, F., Brown, E. Ann., Nibbs, R. J. B., Lyall, H., Sattar, N., Nelson, S. M. & Freeman, D. J. (2018). Pre-conception maternal erythrocyte saturated to unsaturated fatty acid ratio predicts pregnancy after natural cycle frozen embryo transfer. Scientific Reports, 8 (1), Research Online is the open access institutional repository for the University of Wollongong. For further information contact the UOW Library: research-pubs@uow.edu.au

2 Pre-conception maternal erythrocyte saturated to unsaturated fatty acid ratio predicts pregnancy after natural cycle frozen embryo transfer Abstract The environment for embryo implantation and fetal growth and development is affected by matern al nutritional, metabolic and health status. The aim of this prospective, cohort study was to test whether plasma metabolic and inflammatory biomarkers can predict pregnancy resulting from in vitro fertilisation (IVF). Women with a natural menstrual cycle undergoing frozen embryo transfer (FET) were recruited and fasting baseline blood samples were collected a mean of 3.4 days prior to the luteinising hormone (LH) surge and a non-fasting blood sample was taken on the day of FET. Ongoing pregnancy was defined by positive fetal heartbeat on ultrasound scan at day 45 post LH surge. Thirty-six pregnancies resulted from FET in 143 women. In an overall stepwise multivariable analysis, erythrocyte saturated to unsaturated fatty acid ratio was positively associated with ongoing pregnancy. A similar model incorporating day of FET covariates found that erythrocyte saturated to unsaturated fatty acid ratio, erythrocyte fatty acid average chain length and plasma log-triglycerides predicted ongoing pregnancy. In conclusion, a higher peri-conceptional saturated to unsaturated fatty acid ratio predicted ongoing pregnancy after natural cycle frozen embryo transfer and may reflect a maternal nutritional status that facilitates pregnancy success in this assisted conception scenario. Disciplines Medicine and Health Sciences Social and Behavioral Sciences Publication Details Onyiaodike, C. C., Murray, H. M., Zhang, R., Meyer, B. J., Jordan, F., Brown, E. Ann., Nibbs, R. J. B., Lyall, H., Sattar, N., Nelson, S. M. & Freeman, D. J. (2018). Pre-conception maternal erythrocyte saturated to unsaturated fatty acid ratio predicts pregnancy after natural cycle frozen embryo transfer. Scientific Reports, 8 (1), Authors Christopher C. Onyiaodike, Heather Murray, Ruiqi Zhang, Barbara J. Meyer, Fiona Jordan, Elizabeth A. Brown, Robert JB Nibbs, Helen Lyall, Naveed Sattar, Scott M. Nelson, and Dilys J. Freeman This journal article is available at Research Online:

3 Received: 24 August 2017 Accepted: 28 December 2017 Published: xx xx xxxx OPEN Pre-conception maternal erythrocyte saturated to unsaturated fatty acid ratio predicts pregnancy after natural cycle frozen embryo transfer Christopher C. Onyiaodike 1, Heather M. Murray 2, Ruiqi Zhang 2, Barbara J. Meyer 3, Fiona Jordan 4, E. Ann Brown 1, Robert J. B. Nibbs 5, Helen Lyall 1, Naveed Sattar 4, Scott M. Nelson 1 & Dilys J. Freeman 4 The environment for embryo implantation and fetal growth and development is affected by maternal nutritional, metabolic and health status. The aim of this prospective, cohort study was to test whether plasma metabolic and inflammatory biomarkers can predict pregnancy resulting from in vitro fertilisation (IVF). Women with a natural menstrual cycle undergoing frozen embryo transfer (FET) were recruited and fasting baseline blood samples were collected a mean of 3.4 days prior to the luteinising hormone (LH) surge and a non-fasting blood sample was taken on the day of FET. Ongoing pregnancy was defined by positive fetal heartbeat on ultrasound scan at day 45 post LH surge. Thirty-six pregnancies resulted from FET in 143 women. In an overall stepwise multivariable analysis, erythrocyte saturated to unsaturated fatty acid ratio was positively associated with ongoing pregnancy. A similar model incorporating day of FET covariates found that erythrocyte saturated to unsaturated fatty acid ratio, erythrocyte fatty acid average chain length and plasma log-triglycerides predicted ongoing pregnancy. In conclusion, a higher peri-conceptional saturated to unsaturated fatty acid ratio predicted ongoing pregnancy after natural cycle frozen embryo transfer and may reflect a maternal nutritional status that facilitates pregnancy success in this assisted conception scenario. The environment for embryo implantation and fetal growth and development is affected by maternal nutritional, metabolic and health status. Pre-pregnancy body mass index (BMI), to a greater extent than gestational weight gain or dietary intake, influences the maternal metabolic adaptation to pregnancy highlighting the importance of peri-conceptional health 1. In women undergoing assisted reproduction, maternal metabolic health can influence the success of treatment. For example, maternal obesity can affect oocyte and embryo quality 2, uterine receptivity 3 and ultimate success of in vitro fertilisation (IVF) 4. Promotion of a healthy pregnancy in women prior to conception is a potential approach to reduce perinatal morbidity and mortality, however evidence for good biomarkers of peri-conceptional health is lacking. The aspiration is to find new predictive markers that, prior to pregnancy, can identify women at risk of an adverse outcome and identify those who could benefit from lifestyle intervention to improve their peri-conceptional health in both natural conception and IVF scenarios. It has been suggested that metabolites present in the plasma may reflect the environment in the ovary 5. Interestingly, the type of serum fatty acid on day of oocyte retrieval has been associated with pregnancy success with high serum α-linolenic acid levels linked to a lower chance of pregnancy 6. The blood fraction in which fatty acids reside inform us of different aspects of fatty acid metabolism. Plasma fatty acids, predominately derived from phospholipids in lipoproteins, represent plasma fatty acid flux 1 School of Medicine, University of Glasgow, Glasgow, UK. 2 Robertson Centre for Biostatistics, University of Glasgow, Glasgow, UK. 3 School of Medicine, Metabolic Research Centre, University of Wollongong, Wollongong, NSW, Australia. 4 Institute of Cardiovascular and Medical Sciences, University of Glasgow, Glasgow, UK. 5 Institute of Infection, Immunity and Inflammation, University of Glasgow, Glasgow, UK. Correspondence and requests for materials should be addressed to D.J.F. ( dilys.freeman@glasgow.ac.uk) 1

4 Characteristic Pregnant (n = 36) Non-pregnant (n = 107) Odds ratio CI P C statistic Age (years) 35.0 (4.5) 34.7 (4.7) , Current smoker n (%) 4 (11%) 23 (22%) , SIMD quintile 1 Most affluent, 5 most deprived) 1 6 (17%) 21 (20%) , (22%) 21 (20%) , (22%) 32 (30%) , (14%) 15 (14%) , (25%) 18 (17%) referent Systolic blood pressure (mmhg) 116 (13) 115 (12) , Diastolic blood pressure (mmhg) 65 (7) 66 (9) , Height (cm) 165 (7.2) 164 (6.7) , Weight (kg) 71.5 (11.3) 68.4 (13.6) , BMI (kg/m 2 ) 26.5 (4.1) 25.5 (4.7) , Waist (cm) 88.6 (10.6) 85.6 (11.6) , Menstrual period length (days) 4.6 (1.5) 4.8 (1.0) , Cycle length (days) 28.8 (1.74) 28.8 (2.25) , Number of previous pregnancies 24 weeks 0 35 (97%) 102 (98%) >0 1 (3%) 2 (2%) 1.00* Number of previous pregnancies <24 weeks 0 27 (75%) 73 (70%) >0 9 (25%) 31 (30%) , Number of embryos transferred 1 6 (17%) 18 (18%) >1 30 (84%) 83 (82%) , Treatment IVF 13 (36%) 53 (52%) ICSI 23 (64%) 48 (48%) , Table 1. Demographic characteristics and univariate logistic regression models for prediction of ongoing pregnancy at day 45 post-lh surge. * P-value from Fisher s Exact test. Continuous variables are presented as mean (standard deviation) and continuous variables as number (%). Odds ratios are for a 1 unit increase in a variable. BMI = body mass index, CI = confidence interval, IVF = in vitro fertilisation, ICSI = intra-cytoplasmic sperm injection, SIMD = Scottish Index of Multiple Deprivation. Missing data are as follows: n = 1 pregnant (smoking status, systolic blood pressure, diastolic blood pressure, height, weight, cycle length); n = 2 pregnant (weight, BMI); n = 4 pregnant (waist); n = 6 pregnant (menstrual period length); n = 1 non-pregnant (age, smoking status, weight, BMI); n = 2 non-pregnant (waist); n = 3 non-pregnant (cycle length, number of previous pregnancies 24 weeks; number of previous pregnancies <24 weeks); n = 6 non-pregnant (number of embryos transferred; treatment); n = 8 non-pregnant (systolic blood pressure, diastolic blood pressure); n = 13 non-pregnant (menstrual period length). or turnover and are heavily influenced by fasting status. Erythrocyte fatty acid content represents an integrative measure of whole body fatty acid status over the preceding three months (the lifespan of an erythrocyte is 120 days) and erythrocytes may act as a potential storage vehicle for some key polyunsaturated fatty acids such as arachidonic acid (20:4n-6) and docosahexaenoic acid (22:6n-3) 7. Metabolomic profiling has identified maternal plasma fatty acid composition as a potential mediator of the adverse effect of pre-pregnancy BMI on pregnancy outcome 1. Thus markers of nutritional status (such as plasma and erythrocyte fatty acid composition) 8, metabolic and inflammatory markers associated with maternal insulin resistance and obesity 9,10 and inflammatory markers associated with pregnancy loss 11 could be potentially informative for predicting pregnancy success. The aim of this study was to test the ability of metabolic and inflammatory biomarkers to predict ongoing pregnancy after IVF treatment. The study was carried out in a cohort of women presenting for natural cycle frozen embryo transfer (FET). This allowed accurate timing of peri-conceptional sampling and avoided the impact of exogenous hormones on maternal metabolic profile. Methods Patient recruitment. Women, with a regular menstrual cycle (Table 1), undergoing FET in a natural cycle with no exogenous hormones were recruited from the Assisted Conception Unit (ACU) at Glasgow Royal Infirmary between October 2007 and June Indications for IVF treatment are shown in Supplemental Table 1. At recruitment patient demographics, height, weight, waist circumference were collected and Scottish Index of Multiple Deprivation (SIMD) quintile was derived from patient postcode 12. At day 10 after their last 2

5 menstrual period (LMP) date (baseline), the women attended the ACU to commence daily hormonal sampling to detect the LH surge and time embryo (from the woman s own oocyte) replacement. The initial pregnancy test (urinary hcg) was performed on day 18 post-lh surge with confirmation of clinical pregnancy by ultrasound on day 45 post-lh surge. Nine of the women with a negative pregnancy result at day 45 had tested positive on the hcg test at day 18 post-lh surge and were included in the non-pregnant at day 45 post-lh surge group. The study was carried out in accordance with the Declaration of Helsinki (2013). The study was approved by the Glasgow Royal Infirmary Research Ethics Committee (07/S0704/49) and all women in the study provided written informed consent. Blood sampling and biochemical analyses. Baseline blood samples, after at least a 10 hour fast, were collected approximately day 10 after LMP. Date of the LH surge was identified from the daily hormonal sampling and taken as day 0 gestation (Supplemental Figure 1). Baseline samples were collected prior to, or shortly after, LH surge (mean of 3.4 days prior; range 12 days prior to 1 day post). A further non-fasting blood sample was taken on the day of the FET (mean [standard deviation] 2.8 [0.9] days post-lh surge) prior to the procedure. Plasma and erythrocytes were collected after low speed centrifugation and frozen as aliquots at 80 C within 2 hours. Plasma samples were analysed for total cholesterol, triglyceride, HDL cholesterol, glucose, high sensitivity CRP, non-esterified fatty acids (NEFA), insulin, hcg, IL-6, PAI-1, PAI-2 and plasma chemokines (MCP-1 [CCL2], MIP1α [CCL3], MIP1β [CCL4], IL-8 [CXCL8] and eotaxin [CCL11]) as described in the Supplemental Methods. Erythrocyte and plasma fatty analysis was carried out as described in the Supplemental Methods. Statistical analysis. Data are reported as mean (standard deviation) for continuous measures and number (percentage) for categorical measures. Insulin, CRP, triglyceride, IL-6 and IL-8 (CXCL8) were log transformed using the natural logarithm. Missing data are indicated and statistical analysis was carried out on available data. Differences in baseline characteristics between pregnant and non-pregnant groups were tested using two sample t-tests for continuous variables and Fisher s Exact tests for categorical variables. Prediction of successful pregnancy outcome at day 45 post-lh surge was assessed by univariate logistic regression and P < 0.01 was considered significant in univariate analysis to account for multiple testing. Multivariable modelling was carried out over two phases. An initial set of stepwise logistic regression models with P-to-enter and P-to-stay < 0.15 was used to select variables from each of three variable subgroups (metabolic and inflammatory markers; erythrocyte fatty acids; plasma fatty acids) to include in the final stepwise multivariable models. Final stepwise logistic models with P-to-enter < 0.15 and P-to-stay < 0.05 were carried out using the variables selected from the initial models. Results are reported as odds ratio (OR) [95% confidence interval (CI)], Chi square score and associated P-value and the C-statistic for the area under the curve. Odds ratios represent an increase of 1 unit for continuous variables unless otherwise stated. A priori power calculations indicated at least 80% power at the 5% significant level to detect 0.47 fold differences in the majority of parameters tested as a single comparison between successful and failed pregnancy groups with n = 36 pregnancies (Supplemental Methods and Supplemental Table 2). All analyses were performed using the statistical software SAS (Version 9.3, SAS Institute, Cary, NC, USA). Data availability. Data may be available on request, subject to ethical approval for the specific analysis requested, DOI Results Patient characteristics. A total of 196 FET cycles were started in the study, of which 161 were completed. For the purposes of the current analysis, repeat attempts within the study were excluded. There were 143 unique first attempts within the study period from which there were 107 unsuccessful pregnancies and 36 ongoing pregnancies defined by positive fetal heartbeat on ultrasound scan at day 45 post-lh surge (Supplemental Figure 2). Women taking part in the study were on average 35 years of age and had normal blood pressure (Table 1). Women who became pregnant did not differ from those who did not in terms of baseline age, smoking habit, obesity as measured by BMI or waist circumference, or socio-economic status. There were no differences in their previous fertility history. There were no differences between groups in the proportion of women undergoing IVF or intra-cytoplasmic sperm injection (ICSI), nor in the proportions of women having one or more than one embryo transferred. Univariate models for prediction of ongoing pregnancy by baseline demographics and biomarkers. None of the recorded demographic variables predicted ongoing pregnancy at day 45 post-lh surge (Table 1). Baseline erythrocyte unsaturated and saturated fatty acid concentrations were highly correlated with each other (Supplemental Figure 3). When either saturated or unsaturated erythrocyte fatty acids, or their sum (total fatty acid concentration), were individually included in a univariate logistic regression model, none predicted ongoing pregnancy (Table 2). However, if saturated and unsaturated fatty acids were entered simultaneously into a multivariable model, each was a highly significant predictor (Combined model 1, Table 2). This suggests that saturated (positive direction) and unsaturated (negative direction) fatty acids have significant individual effects that are dependent on each other. To establish the best way to capture this information, a number of univariate and multivariable models incorporating the total of saturated and unsaturated fatty acids, their difference and their ratio were carried out (Table 2). The model with the lowest Akaike Information Criterion (97.4) was the ratio of saturated to unsaturated fatty acids alone and this measure was used in all univariate and multivariate models. Analysis of baseline plasma, day of FET erythrocyte and day of FET plasma saturated and unsaturated fatty acids yielded similar results (data not shown) and their ratios were similarly used in logistic regression analysis. 3

6 Variable Odds ratio CI P C statistic AIC Univariate models Saturated fatty acid (nmol/ml) , Unsaturated fatty acid (nmol/ml) , Total fatty acid (nmol/ml) , Saturated/unsaturated fatty acid ratio (0.05 units) , Saturated unsaturated fatty acid difference (nmol/ml) , Combined model Saturated fatty acid (nmol/ml) , Unsaturated fatty acid (nmol/ml) , Combined model Total fatty acid (nmol/ml) , Saturated unsaturated fatty acid difference (nmol/ml) , Combined model Total fatty acids (nmol/ml) , Saturated/unsaturated fatty acid ratio (0.05 units) , Table 2. Logistic regression models incorporating measures of baseline erythrocyte saturated and unsaturated fatty acids for prediction of ongoing pregnancy at day 45 post LH-surge. Odds ratios are for a 1 unit increase in a variable. AIC = Akaike Information Criterion, CI = confidence interval. Plasma and erythrocyte fatty acid data was missing for n = 1 non-pregnant participant. Baseline concentrations of plasma metabolic and inflammatory biomarkers (Table 3, Supplemental Table 3), erythrocyte fatty acids and summary measures (Table 3, Supplemental Tables 4 and 5) and plasma fatty acids and summary measures (Table 3, Supplemental Tables 6 and 7) are shown. Univariate analysis was carried out and variables that predicted ongoing pregnancy with P < 0.15 are shown in Table 3. These variables were included in multivariable analysis. On univariate analysis only baseline erythrocyte saturated to unsaturated fatty acid ratio predicted ongoing success at day 45 post-lh surge (P < 0.001) (Supplemental Figure 4). Univariate models for prediction of successful pregnancy outcome by day of FET, non-fasting biomarkers. Non-fasting blood samples were taken just prior to the FET procedure on median day 3 post-lh surge and metabolic and inflammatory biomarkers assessed as well as erythrocyte and plasma fatty acids (data not shown). Day of FET non-fasting plasma log-triglyceride (3.48 [1.43, 8.48], P = 0.006, C-statistic 0.66) was associated with a higher odds of ongoing pregnancy and additionally insulin, CRP and NEFA (all P < 0.15) were included in stepwise multivariable modelling. Erythrocyte 14:0 (1.09 [1.02, 1.16], P = , C-statistic 0.69), n-6 polyunsaturated fatty acids (PUFA) (0.56 [0.36, 0.86], P = , C-statistic 0.68), average chain length (0.51, [0.33, 0.76], P = , C-statistic 0.73) and saturated/unsaturated fatty acid ratio (0.05 units) (6.70, [2.25, 19.88], P = , C-statistic 0.73) were associated with ongoing pregnancy. Additionally erythrocyte 14:1 n-7, 18:1 n-7, 18:3 n-3, 20:1 n-9, 20:3 n-6, 20:4 n-6, 22:4 n-6, 22:5 n-3, 24:1 n-9, unsaturated index and % C20 22 fatty acids (all P < 0.15) were included in stepwise multivariable modelling. Plasma 14:0 (1.01 [1.00, 1.02], P = 0.008, C-statistic 0.66), 17:0 (1.07 [1.02, 1.12], P = 0.007, C-statistic 0.68), plasma saturated/unsaturated fatty acid ratio (0.05 units) (2.31 [1.30, 4.09], P = , C-statistic 0.69), n-6 PUFA (0.80 [0.68, 0.93] P = , C-statistic 0.70), unsaturated index (0.91 [0.84, 0.98], P = , C-statistic 0.68) and fatty acid average chain length (0.05 units) (0.65 [0.48, 0.88], P = , C-statistic 0.70) were associated with ongoing pregnancy. Plasma 12:0, 14:1 n-7, 16:0, 16:1 n-7; 18:0, 18:1 n-9, 20:1 n-9, 24:1 n-9 and % C20 22 fatty acids (all P < 0.15) were also included in stepwise multivariable modelling. Multivariable models for prediction of ongoing pregnancy. Initial stepwise multivariable models (P-to-enter, P-to-stay < 0.15) were carried out using the baseline and day of FET variables, selected at P < 0.15 on univariate analysis (Table 3 for baseline measures and listed above for day of FET measures). This resulted in the selection of log triglyceride, log IL-6, log insulin, erythrocyte saturated to unsaturated fatty acid ratio, average chain length, 18:3 n-3, 20:1 n-9 and 24:1 n-9; and plasma saturated to unsaturated fatty acid ratio, 17:0 and 24:1 n-9 for inclusion in the final stepwise models. Table 4 shows the final multivariable logistic regression models for predictors of ongoing pregnancy. The only baseline predictor of ongoing pregnancy remaining after stepwise multivariable analysis (P-to-enter < 0.15, P-to-stay < 0.05) was saturated to unsaturated fatty acid ratio with an odds ratio of 7.19 (2.33, 22.17) per 0.05 unit increase, P = The C-statistic for this prediction model was When non-fasted day of FET covariates were used to predict ongoing pregnancy, erythrocyte saturated to unsaturated fatty acid ratio OR 7.11 (2.05, 24.71) per 0.05 unit increase, P = 0.002, erythrocyte fatty acid average chain length OR 0.58 (0.37, 0.92) per 0.05 unit increase, P = and plasma log-triglycerides OR 4.23 (1.05, 16.95) P = predicted ongoing pregnancy. The C-statistic for the prediction model based on non-fasting day of FET covariates was

7 Variable Pregnant (n = 36) Non-pregnant (n = 107) Odds Ratio Confidence Interval P C-statistic Plasma metabolic and inflammatory markers Triglyceride* (mmol/l) 1.01 (1.47) 0.90 (1.48) 2.15 (0.82, 5.63) IL-6* (pg/ml) 1.14 (2.01) 0.90 (1.76) 1.86 (0.93, 3.72) Erythrocyte fatty acids and summary measures 14:0 (nmol/ml) 12.8 (7.06) 9.60 (6.35) , :0 (nmol/ml) 6.94 (3.06) 5.72 (2.04) , :1 n-7 (nmol/ml) 6.31 (8.89) (10.98) , :1 n-9 (nmol/ml) 4.24 (1.06) 4.94 (1.35) , :1 n-9 (nmol/ml) 64.5 (9.8) 70.2 (12.6) , :2 n-6 (nmol/ml) 0.66 (1.24) 1.30 (1.77) , :4 n-6 (nmol/ml) 38.5 (6.7) 43.1 (10.3) , :3 n-3 (nmol/ml) 3.93 (2.08) 2.95 (2.22) , % Total n (1.27) 28.4 (1.3) , Unsaturated Index (4.1) (4.0) , Average chain length (0.05 units) (0.05) (0.06) , Saturated/unsaturated ratio (0.05 units) 0.78 (0.02) 0.76 (0.02) , Plasma fatty acids and summary measures 14:0 (nmol/ml) 118 (51) 98.3 (45.8) , :0 (nmol/ml) 30.0 (10.1) 25.6 (9.8) , :0 (nmol/ml) 618 (117) 577 (98) , :1 n-7 (nmol/ml) 41.7 (35.1) 29.6 (29.1) , :1 n-9 (nmol/ml) 88.5 (10.4) 94.3 (15.9) , Saturated/unsaturated ratio (0.05 units) 0.52 (0.05) 0.49 (0.04) , % Total n (3.09) 38.5 (3.4) , Average chain length (0.05 units) (0.08) (0.09) , Table 3. Baseline variable concentrations and univariate logistic regression models for prediction of ongoing pregnancy at day 45 post-lh surge where P < Continuous variables are presented as mean (standard deviation) or * geometric means (standard deviation). Odds ratios are for a 1 unit increase in a variable apart from. IL-6 = interleukin 6. IL-6 data was missing for n = 1 pregnant and n = 50 non-pregnant participants. Plasma triglyceride and plasma and erythrocyte fatty acid data was missing for n = 1 non-pregnant participant. Discussion In women undergoing natural cycle FET, the erythrocyte ratio of saturated to unsaturated fatty acids prior to pregnancy predicted ongoing pregnancy at day 45 post-lh surge. Similarly, on day of FET, higher erythrocyte saturated to unsaturated fatty acid ratio and shorter average chain length, in addition to higher concentrations of plasma triglycerides, predicted ongoing pregnancy. Erythrocytes have a lifespan of 120 days, though their fatty acid composition may reflect more recent changes in concentration 13. As erythrocyte composition reflects that of tissue stores 14, this suggests that the quality of stored fatty acids has an impact on pregnancy outcome. A higher ratio of saturated to unsaturated fatty acids in the diet is commonly linked to metabolic diseases such as coronary heart disease, however this issue is complex and there is a lack of consensus on the scientific evidence for these associations 15,16. Erythrocyte fatty acid composition is unaffected by fasting status, thus it is reassuring to see similar erythrocyte predictors at both time points. The prediction of ongoing pregnancy by non-fasting plasma triglyceride levels on day of FET, which are increased post-prandially, suggests that pre-implantation maternal handling of dietary lipids may be important in the preparation for pregnancy. Serum fatty acids have previously been linked to pregnancy outcome in an IVF population. Higher serum α-linolenic acid concentrations collected on day of oocyte retrieval were associated with a lower chance of pregnancy, although this may not be independent of endometriosis 6. These data were not replicated in a subsequent study which instead found a high linoleic acid to α-linolenic acid ratio was associated with pregnancy success after adjustment for confounders 17. Taken together with our study, these preliminary data indicate that the proportions of plasma fatty acids of different functionality may be an important factor in pregnancy success. Maternal metabolic status could influence fertility by improving oocyte/embryo quality or endometrial receptivity. The mammalian blastocyst is dependent on its own energy stores to drive cell division 18 and endogenous triglycerides are a key energy source during oocyte maturation and preimplantation embryo development 19. Mouse models suggest that fatty acid oxidation is critical for oocyte function, embryo development, implantation and endometrial stromal cell decidualisation 20,21. In human oocytes 80% of fatty acids are saturated 22,23. Exposure of the blastocyst to external lipid appears to be cytotoxic 24 which suggests that fatty acid supply via uterine secretions may not occur. Human embryos that successfully develop to blastocysts have lower triglyceride content, although it is difficult to determine if this is due to less initial storage or greater oxidation rates providing more energy while the blastocyst was being formed 2. A well-stocked oocyte in terms of lipid available for oxidation (i.e. saturated fat) may have an advantage over one that lacks such lipid. 5

8 Multivariable Visit/Covariate Odds Ratio (95% confidence interval) P-value C-statistic (Area Under Curve) Baseline (mean of 3.4 days prior to LH surge) Erythrocyte saturated/unsaturated fatty acid ratio (0.05 units) 7.19 (2.33, 22.17) Day of FET (mean of 2.8 days post-lh surge) Erythrocyte saturated/unsaturated fatty acid ratio (0.05 units) 7.11 (2.05, 24.71) Erythrocyte fatty acid average chain length (0.05 units) 0.58 (0.37, 0.92) Log [triglyceride (mmol/l)] 4.23 (1.05, 16.95) Table 4. Multivariable logistic regression models for predicting pregnancy success at 45 days post-lh surge. Covariates to enter the stepwise logistic regression were initially selected using baseline, or day of FET (mean 2.8 days post-lh surge) stepwise logistic regression models run for all covariates (plasma and erythrocyte metabolic, inflammatory and fatty acid markers) P-to-enter and P-to-stay < 0.15). Variables included were baseline or day of FET triglyceride, IL-6 and insulin; erythrocyte 18:3 n-3, 20:1 n-9, 24:1 n-9, saturated/ unsaturated fatty acid ratio and average chain length; and plasma 17:0, 24:1 n-9 and saturated/unsaturated fatty acid ratio. The final multivariable model is a stepwise model with P-to-enter and P-to-stay < Data shown are the odds ratio (for the given unit of change), and the 95% confidence interval, with the associated Chi square score, P-value, and the C-statistic (for the Area under the Curve). FET = frozen embryo transfer. The oocytes in the current study were retrieved at least three months prior to FET so our baseline biomarkers do not necessarily reflect the environment of oocyte development but will reflect the implantation environment. Uterine receptivity is affected by changes in metabolic environment associated with maternal obesity or polycystic ovarian syndrome (PCOS) (reviewed in 25 ). Pregnancy hormones influenced adipokine secretion from and increased lipolysis in an adipocyte cell line 26. The pregnancy hormone-conditioned medium from these adipocytes increased receptivity marker expression in an endometrial cell line 26. Adipocytes contain predominately saturated fatty acids whose composition reflects that of plasma fatty acids which in turn are influenced by dietary intake 27. A significant increase in fatty acid release into plasma from adipocytes may lead to an increase in the saturated to unsaturated fatty acid ratio. Weight loss in overweight women with PCOS increased endometrial expression of genes involved in glucose homeostasis, which may improve endometrial receptivity 28. Improvements in insulin sensitivity at the endometrium may also enhance tissue fatty acid uptake and oxidation. The analysis of non-fasting biomarkers taken immediately prior to FET produced a predictive model with a higher C statistic than that using fasting biomarkers pre-lh surge. Erythrocyte fatty acid average chain length appeared as a negative predictor reinforcing the suggestion that shorter chain fatty acids commonly used in energy storage are important for pregnancy success. It is interesting that higher plasma triglyceride concentrations were predictive of an ongoing pregnancy in the non-fasting biomarker analysis. Plasma triglyceride concentrations under these conditions represent recent post prandial intake of lipid. This might suggest that women with higher plasma levels of dietary lipids may be have better endometrial receptivity or improved oocyte quality. A recent report that higher dietary dairy intake was associated with increased live birth rate in an IVF population, at least in older women, may support this concept 29. FET of autologous or donor embryos comprises 37.7% of assisted conception cycles currently undertaken in the United Sates (CDC Assisted Reproductive Technology Annual Report 2015). Biomarkers, which can indicate the potential for the success of different assisted conception protocols, are helpful both in tailoring treatment to the individual patients and in devising intervention strategies, e.g. dietary supplementation or intervention, that may improve success rates in assisted conception treatment. One strength of our study was the prospective design in an IVF population allowing accurate peri-conceptional timing of sampling. All women had a natural menstrual cycle and results were free from the impact of exogenous hormones. Use of frozen, rather than fresh, embryos may mean our results are specific to frozen embryos. We did not observe the expected associations between maternal BMI, smoking or number of embryos transferred 30 and ongoing pregnancy probably due to the small number of pregnancies and the limited BMI range within our population limiting statistical power. Pregnancy success rates in this study were low reflecting day 3 embryo transfers and the absence of pre-implantation genetic screening. Nine women who tested positive for pregnancy on hcg test at day 18 post-lh surge, but negative at day 45 post-lh surge ultrasound scan were included as unsuccessful pregnancies. These women may represent a particular subgroup where implantation is successful, but pregnancy maintenance is not. This group was too small to be analysed separately and as our a priori pregnancy outcome was positive fetal heartbeat at day 45 post-lh surge we included them in our unsuccessful pregnancy group. We cannot determine whether the relationships we observed only occur under conditions of compromised fertility or extend to natural conception in the wider population. The peri-conceptional period is a key opportunity for interventional strategies that is routinely missed due to management of most pregnancies commencing at antenatal booking around weeks of gestation. Evidence-based strategies, delivered either in the preconception or antenatal periods, to reduce adverse pregnancy outcomes are being sought. If our results are validated, the saturated to unsaturated fat ratio in erythrocytes could be explored as a marker of maternal metabolic health. However it should be noted that the absolute difference in saturated to unsaturated fatty acid ratio between groups was very small and while it may suggest potential biological metabolic pathways of importance in conception it is unlikely to be a good biomarker in itself. 6

9 In summary, higher peri-conceptional saturated to unsaturated fatty acid ratio predicted ongoing pregnancy after natural cycle FET and may reflect a maternal nutritional status that facilitates pregnancy success in this assisted conception scenario. Dietary interventions could be used to manipulate the saturated to unsaturated fatty acid ratio and better assess its effect on pregnancy outcome both in assisted conception and free-living populations. References 1. Hellmuth, C. et al. Association of maternal prepregnancy BMI with metabolomic profile across gestation. International journal of obesity (2005) 41, , (2017). 2. Leary, C., Leese, H. J. & Sturmey, R. G. Human embryos from overweight and obese women display phenotypic and metabolic abnormalities. Human reproduction (Oxford, England) 30, , (2015). 3. Bellver, J. et al. Obesity reduces uterine receptivity: clinical experience from 9,587 first cycles of ovum donation with normal weight donors. Fertility and sterility 100, , (2013). 4. Provost, M. P. et al. Pregnancy outcomes decline with increasing recipient body mass index: an analysis of 22,317 fresh donor/ recipient cycles from the Society for Assisted Reproductive Technology Clinic Outcome Reporting System registry. Fertility and sterility 105, , (2016). 5. Robker, R. L. et al. Obese women exhibit differences in ovarian metabolites, hormones, and gene expression compared with moderate-weight women. The Journal of clinical endocrinology and metabolism 94, , (2009). 6. Jungheim, E. S., Macones, G. A., Odem, R. R., Patterson, B. W. & Moley, K. H. Elevated serum alpha-linolenic acid levels are associated with decreased chance of pregnancy after in vitro fertilization. Fertility and sterility 96, , fertnstert (2011). 7. Ghebremeskel, K. et al. Blood fatty acid composition of pregnant and nonpregnant Korean women: red cells may act as a reservoir of arachidonic acid and docosahexaenoic acid for utilization by the developing fetus. Lipids 35, (2000). 8. Stewart, F. et al. Longitudinal assessment of erythrocyte fatty acid composition throughout pregnancy and post partum. Lipids 42, , (2007). 9. Nelson, S. M., Matthews, P. & Poston, L. Maternal metabolism and obesity: modifiable determinants of pregnancy outcome. Human reproduction update 16, , (2010). 10. Ramsay, J. E. et al. Maternal obesity is associated with dysregulation of metabolic, vascular, and inflammatory pathways. The Journal of clinical endocrinology and metabolism 87, (2002). 11. Madigan, J. et al. Chemokine scavenger D6 is expressed by trophoblasts and aids the survival of mouse embryos transferred into allogeneic recipients. Journal of immunology (Baltimore, Md.: 1950) 184, , (2010). 12. Scottish Index of Multiple Deprivation2004: Summary Technical Report. Scottish Executive. Edinburgh. (Scottish Executive, Edinburgh, 2004). 13. Skeaff, C. M., Hodson, L. & McKenzie, J. E. Dietary-induced changes in fatty acid composition of human plasma, platelet, and erythrocyte lipids follow a similar time course. The Journal of nutrition 136, (2006). 14. Arab, L. Biomarkers of fat and fatty acid intake. The Journal of nutrition 133(Suppl 3), 925S 932S (2003). 15. Hammad, S., Pu, S. & Jones, P. J. Current Evidence Supporting the Link Between Dietary Fatty Acids and Cardiovascular Disease. Lipids 51, , (2016). 16. Ramsden, C. E. et al. Re-evaluation of the traditional diet-heart hypothesis: analysis of recovered data from Minnesota Coronary Experiment ( ). BMJ (Clinical research ed.) 353, i1246, (2016). 17. Jungheim, E. S., Frolova, A. I., Jiang, H. & Riley, J. K. Relationship between serum polyunsaturated fatty acids and pregnancy in women undergoing in vitro fertilization. The Journal of clinical endocrinology and metabolism 98, E , org/ /jc (2013). 18. Gardner, D. K. Changes in requirements and utilization of nutrients during mammalian preimplantation embryo development and their significance in embryo culture. Theriogenology 49, (1998). 19. Ferguson, E. M. & Leese, H. J. A potential role for triglyceride as an energy source during bovine oocyte maturation and early embryo development. Molecular reproduction and development 73, , (2006). 20. Dunning, K. R. et al. Beta-oxidation is essential for mouse oocyte developmental competence and early embryo development. Biol Reprod 83, , (2010). 21. Tsai, J. H., Chi, M. M., Schulte, M. B. & Moley, K. H. The fatty acid beta-oxidation pathway is important for decidualization of endometrial stromal cells in both humans and mice. Biol Reprod 90, 34, (2014). 22. Kim, J. Y., Kinoshita, M., Ohnishi, M. & Fukui, Y. Lipid and fatty acid analysis of fresh and frozen-thawed immature and in vitro matured bovine oocytes. Reproduction 122, (2001). 23. Matorras, R. et al. Fatty acid composition of fertilization-failed human oocytes. Human reproduction (Oxford, England) 13, (1998). 24. Cagnone, G. & Sirard, M. A. The impact of exposure to serum lipids during in vitro culture on the transcriptome of bovine blastocysts. Theriogenology 81, e , (2014). 25. Schulte, M. M., Tsai, J. H. & Moley, K. H. Obesity and PCOS: the effect of metabolic derangements on endometrial receptivity at the time of implantation. Reprod Sci 22, 6 14, (2015). 26. Gamundi-Segura, S., Serna, J., Oehninger, S., Horcajadas, J. A. & Arbones-Mainar, J. M. Effects of adipocyte-secreted factors on decidualized endometrial cells: modulation of endometrial receptivity in vitro. Journal of physiology and biochemistry 71, , (2015). 27. Hodson, L. et al. Micro-techniques for analysis of human adipose tissue fatty acid composition in dietary studies. Nutr Metab Cardiovasc Dis 23, , (2013). 28. Ujvari, D. et al. Lifestyle intervention up-regulates gene and protein levels of molecules involved in insulin signaling in the endometrium of overweight/obese women with polycystic ovary syndrome. Human reproduction (Oxford, England) 29, , (2014). 29. Afeiche, M. C. et al. Dairy intake in relation to in vitro fertilization outcomes among women from a fertility clinic. Human reproduction (Oxford, England) 31, , (2016). 30. Lawlor, D. A. & Nelson, S. M. Effect of age on decisions about the numbers of embryos to transfer in assisted conception: a prospective study. Lancet 379, , (2012). Acknowledgements We acknowledge the generous support of: The Wellbeing of Women/Royal College of Obstetrics and Gynaecology Research Grant (RG/939/07), The Biochemical Society Travel Grant (101008), The Carnegie Trust, The Harold Hyam Wingate Foundation and University of Wollongong Study Leave Assistance Grants. 7

10 Author Contributions C.C.O. recruited patients, collected blood samples and patient information, assisted in data analysis and interpretation. H.M.M. statistical analysis. R.Z. statistical analysis. B.J.M. fatty acid GC profiling, data analysis and interpretation, assisted in manuscript preparation. F.J. inflammatory marker analysis. E.A.B. collected blood samples, fatty acid extractions, ELISA and plasma lipid analyses. R.J.B.N. conceived idea, secured funding, contributed to data interpretation. H.L. facilitated patient recruitment, secured funding, contributed to data interpretation. N.S. - conceived idea, secured funding, contributed to data interpretation. S.M.N. clinical lead, facilitated patient recruitment, conceived idea, secured funding, assisted in manuscript preparation. D.J.F. principal investigator and supervisor for C.C.O., data analysis, manuscript preparation, conceived idea and secured funding. Additional Information Supplementary information accompanies this paper at Competing Interests: The authors declare that they have no competing interests. Publisher's note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit The Author(s)