4 Columns for HTS and LC/MS Analysis

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1 YMC GENERAL CATALG Columns for HTS and LC/MS Analysis Semi-micro columns for LC/MS YMC-UltraHT series YMC CombiChrom ,99 93 第章.indd :51:39 AM

2 Semi-micro columns for LC/MS Wide selection of packing materials and column sizes Most suitable for microanalysis S e m i - m i c r o c o l u m n s o p t i m i z e d f o r L C / M S YMC Co., Ltd. s Semi-micro columns are high performance columns, providing good peak shapes when used with mobile phase suitable for LC/MS. They come in a wide range of column sizes and with a wide selection of packing materials. This enables column selection according to the intended purpose, such as rapid analysis or precise separation. Abundant packing material types Packing material Triart C18 Pro C18 Hydrosphere C18 Pro C18 RS Pro C8 Pro C TMS Ph CN Characteristics Versatile hybrid silica based DS column, great chemical durability, excellent peak shape in every condition High performance DS packing material Can be used with 1 water mobile phase Superior separation for hydrophilic compounds High carbon DS packing material, high durability Separation behavior may differ from DS, since stationary phase hydrophobicity is lower than DS Have available π electrons derived from functional groups and show different separation characteristics from DS. π-π interactions For details about characteristics of these and other packing materials, see pages for each column. Reduced column bleed 1.E+9 8.E+8 TIC Triart C18 No column Bleeding of stationary phases can often contribute to high back ground signals when employing MS and other hyphenated detection techniques. YMC Triart C18 column shows no contribution to the signal observed by the MS positive total ion current (TIC) trace. Such performance allows for low limits of detection (high S/N ratio) for MS and other detection techniques..e+8.e min Column : YMC-Triart C18 (5 µm, 1 Å) 5. mmi.d. Eluent : A) water/formic acid (1/.1) B) acetonitrile/formic acid (1/.1) 5 B (-1 min), 5-1 B (1-5 min), 1 B (5-1 min), 1-5 B (1-1.1 min), 5 B ( min) Flow rate :. ml/min Temperature : Detection :ESI positive, TIC (m/z 5-1) A p p l i c a t i o n mau 1 5 Melamine-spiked pet food (1 mg/kg, final.5 µg/ml) NH N N H N N NH Melamine Sample preparation method Pet food (1 g) 5 aqueous acetonitrile (5 ml) Sonication for 3 min Centrifugation at 1, rpm for 1 min Filtration Dilution times with eluent A Filtration Injection min (R819D) Melamine in pet food, conditions for LC/MS Column : YMC-UltraHT Hydrosphere C18 ( µm, 1 Å) 5. mmi.d. Eluent : A) water/heptafluorobutyric acid (1/.1) B) methanol/heptafluorobutyric acid (1/.1) 5 B (-.17 min), 5-9 B (.17-3 min) Flow rate :. ml/min Temperature : Detection :UV at nm Injection :1 µl Melamine in pet food was analyzed with volatile eluent for LC/MS analysis. Hydrosphere C18 is suitable LC/MS column for hydrophilic compounds that are difficult to retain in reversed phase conditions. 9 第章.indd :51:1 AM

3 A p p l i c a t i o n Intens. sample_1ul_-c,5_1_753.d: BPC All MS x1 (S9111A) LC/TF-MS analysis of the in vitro metabolites of diltiazem in human liver S9 metabolites unchanged DTZ Diltiazem HCl (DTZ) min Intens. x1 BPC sample_1ul_-c,5_1_753.d: BPC All MS x1 EIC [unchanged DTZ] sample_1ul_-c,5_1_753.d: EIC 15.19±.1 +All MS x1 x1 x1 EIC [-CH] EIC [-CCH] EIC [-(CH)] EIC [-C(CH)] EIC [-C(CH)3] sample_1ul_-c,5_1_753.d: EIC 1.153±.1 +All MS sample_1ul_-c,5_1_753.d: EIC ±.1 +All MS sample_1ul_-c,5_1_753.d: EIC ±.1 +All MS sample_1ul_-c,5_1_753.d: EIC 359.1±.1 +All MS sample_1ul_-c,5_1_753.d: EIC 35.17±.1 +All MS min A p p l i c a t i o n Courtesy of J.Watanabe, Bruker Daltonics K. K. Column : YMC-UltraHT Hydrosphere C18 ( µm, 1 Å) 5. mmi.d. Eluent : A) mm HCNH, B) methanol 5 B (-.5 min), 5-8 B ( min), 8 B (3.5- min) Flow rate :. ml/min Temperature : 37 Detection : ESI, positive mode, m/z 1-1 (Bruker Daltonics micrtf II) YMC-UltraHT Hydrosphere C18 is suitable for ultra fast LC analysis of hydrophilic compounds like drug metabolites. It is possible to analyze in short time due to the combination high resolution µm column and TF-MS analysis. (F118B) Diode Array 7 nm 1.5e3 min TIC 1.e7 m/z 1 1.8e m/z 5 1.8e m/z e LC/MS analysis of tetracycline antibiotics 3 1 H H3C xytetracycline (MW.) HC 3 H3C Tetracycline (MW.) Cl HC 3 Chlortetracycline (MW 78.8) N H3C H3C N N N NH NH NH X peak area 1 R = concentration (µg/ml) min NH Column : YMC-Triart C18 (5 µm, 1 Å) 5. mmi.d. Eluent : acetonitrile/water/formic acid (15/85/.1) Flow rate :. ml/min Temperature : Detection : A) UV at 7 nm B) ESI positive-mode Injection : 1 µl (.-1 µg/ml) Doxycycline (MW.) Triart C18 shows no adsorption for coordinate compound of tetracyclines with good peak shape and quantitative. Therefore, it is applicable for high sensitive analysis. 95 第章.indd :51:5 AM

4 Analytical columns Semi-micro columns for LC/MS rdering information Semi-micro column for LC/MS(See P,7 for µm of UltraHT Series) Packing material Particle size Pore size (µm) (Å) Column size inner diameter length(mm) Triart C18 S TA1S3-3WT. 5 TA1S3-5WT. 75 TA1S3-L5WT. 1 TA1S3-1WT. 15 TA1S3-15WT S TA1S5-3WT. 5 TA1S5-5WT. 75 TA1S5-L5WT. 1 TA1S5-1WT. 15 TA1S5-15WT Pro C18 S AS1S3-H51WT 1. 5 AS1S3-51WT 1. 1 AS1S3-11WT AS1S3-H5P5WT AS1S3-5P5WT AS1S3-1P5WT. 35 AS1S3-H5WT. 5 AS1S3-5WT. 75 AS1S3-L5WT. 1 AS1S3-1WT. 15 AS1S3-15WT S AS1S5-151WT 1. 5 AS1S5-51WT AS1S5-15P5WT AS1S5-5P5WT. 35 AS1S5-H5WT. 5 AS1S5-5WT. 75 AS1S5-L5WT. 1 AS1S5-1WT. 15 AS1S5-15WT. 5 AS1S5-5WT Hydrosphere C18 S HS1S3-H51WT 1. 5 HS1S3-51WT 1. 1 HS1S3-11WT HS1S3-H5P5WT HS1S3-5P5WT HS1S3-1P5WT. 35 HS1S3-H5WT. 5 HS1S3-5WT. 75 HS1S3-L5WT. 1 HS1S3-1WT. 15 HS1S3-15WT S HS1S5-151WT 1. 5 HS1S5-51WT HS1S5-15P5WT HS1S5-5P5WT. 35 HS1S5-H5WT. 5 HS1S5-5WT. 75 HS1S5-L5WT. 1 HS1S5-1WT. 15 HS1S5-15WT. 5 HS1S5-5WT Packing material Particle size Pore size (µm) (Å) Column size inner diameter length(mm) Pro C18 RS S RS8S3-H51WT 1. 5 RS8S3-51WT 1. 1 RS8S3-11WT RS8S3-H5P5WT RS8S3-5P5WT RS8S3-1P5WT. 35 RS8S3-H5WT. 5 RS8S3-5WT. 75 RS8S3-L5WT. 1 RS8S3-1WT. 15 RS8S3-15WT S RS8S5-151WT 1. 5 RS8S5-51WT RS8S5-15P5WT RS8S5-5P5WT. 35 RS8S5-H5WT. 5 RS8S5-5WT. 75 RS8S5-L5WT. 1 RS8S5-1WT. 15 RS8S5-15WT. 5 RS8S5-5WT Pro C8 S S1S3-H5WT. 5 S1S3-5WT. 75 S1S3-L5WT S S1S5-151WT 1. 5 S1S5-51WT S1S5-15P5WT S1S5-5P5WT. 5 S1S5-5WT. 75 S1S5-L5WT. 1 S1S5-1WT. 15 S1S5-15WT Pro C S BS1S3-H5WT. 5 BS1S3-5WT. 75 BS1S3-L5WT S BS1S5-151WT 1. 5 BS1S5-51WT BS1S5-15P5WT BS1S5-5P5WT. 5 BS1S5-5WT. 75 BS1S5-L5WT. 1 BS1S5-1WT. 15 BS1S5-15WT 9 第章.indd :51: AM

5 YMC-UltraHT series YMC-UltraHT series are C18 bonded-phases based on highly efficient µm spherical silica particles. YMC-UltraHT series columns are specially designed for high-speed and high throughput analysis in various fields such as pharmaceuticals, foods, and environmental. The YMC-UltraHT series have two See P 7 for details of YMC-UltraHT series. products; Pro C18 is as our standard DS, and Hydrosphere C18 for hydrophilic compounds. Both show the same selectivity and superior peak shapes as 3 µm and 5 µm Pro series, so easy method transfer from conventional HPLC to ultra-fast LC is available without changing elution conditions. Simultaneous analysis for agricultural chemicals by LC/MS YMC-Pack Pro C mmi.d. 3 µm Gradient : 15- B (-1 min), B (1-3.5 min), -5 B (3.5- min), 5-55 B (-8 min), B ( min), 95 B (17.5- min) Flow rate :. ml/min Intensity, cps Intensity, cps 9.5e5 9.e5 8.5e5 8.e5 7.5e5 7.e5.5e5.e5 5.5e5 5.e5.5e5.e5 3.5e5 3.e5.5e5.e5 1.5e5 1.e5 5.e. Gradient Flow rate 1.e 9.e5 8.e5 7.e5.e5 5.e5.e5 3.e5.e5 1.e5. XIC of +MRM (5 pairs) YMC-UltraHT Pro C mmi.d. µm : 15- B (-.5 min), B ( min), -5 B ( min), 5-55 B (3- min), B (-8.75 min), 95 B ( min) :. ml/min XIC of +MRM (5 pairs) , Time, min 9 1, Eluent : A) 5 mm CNH in water B) 5 mm CNH in methanol Temperature :ambient Detection :ESI, Positive, MRM (API5) Sample : Pesticide Mixture Standard Solution PL-7-, manufactured by Wako Pure Chemical Industries, Ltd Time, min 7 analysis time 1/ , , Max..8e5 cps. Max. 3.e5 cps. Courtesy of J.Watanabe, TAKARA BI INC. 1. Thiamethoxam (Q1/Q3 : 9.3/11.3). Clothianidin (Q1/Q3 : 5.3/19.1) 3. Chloridazon (Q1/Q3 :.3/77.). Thiacloprid (Q1/Q3 : 53.3/1.) 5. Thiabendazole (Q1/Q3 :.3/175.). Azamethiphos (Q1/Q3 : 35./183.) 7. Dimethirimol (Q1/Q3 : 1./71.1) 8. Isoxaflutole (Q1/Q3 : 3./51.1) 9. Pyriftalid (Q1/Q3 : 319.3/139.1) 1. (E)-Ferimzone (Q1/Q3 : 55./91.1) 11. (Z)-Ferimzone (Q1/Q3 : 55./91.1) 1. Methoxyfenozide (Q1/Q3 : 39./19.3) 13. Iprovalicarb (Q1/Q3 : 31./119.3) 1. Chromafenozide (Q1/Q3 : 395./175.1) 15. Butafenacil (Q1/Q3 : 9.1/331.1) 1. Simeconazole (Q1/Q3 : 9.3/7.1) 17. Cyazofamid (Q1/Q3 : 35./18.) 18. Naproanilide (Q1/Q3 : 9.3/171.3) 19. Fenoxycarb (Q1/Q3 : 3.3/88.1). Anilofos (Q1/Q3 : 38./199.1) 1. Cyflufenamid (Q1/Q3 : 31.3/95.). Pyrazolynate (Q1/Q3 : 39.1/91.) 3. Indoxacarb (Q1/Q3 : 58.1/3.). Benzofenap (Q1/Q3 : 31./15.1) 5. Furathiocarb (Q1/Q3 : 383.3/195.). Cloquintocet-mexyl (Q1/Q3 : 33.3/38.) Shown in above chromatogram, the compounds of agricultual chemicals were analyzed on Pro C18 column (3 µm, 15. mmi.d.). Every basic compound shows good peak shape. Below chromatogram was obtained with UltraHT Pro C18 ( µm, 75. mmi.d.), it shows same separation pattern with short time analysis. 97 第章.indd :5:3 AM

6 High throughput column YMC CombiChrom Column with a particle size of 5 µm and a length of 5 mm for high-speed separation The most suitable column design for gradient analysis Excellent durability F o r s c r e e n i n g o r i s o l a t i o n o f c o m b i n a t o r i a l c h e m i s t r y c o m p o u n d s YMC CombiChrom is intended for screening or isolation of combinatorial chemistry compounds. Its excellent durability enables it to be used under severe conditions, including high flow rate, and it may be scaled-up from analytical to preparative. High-speed separation using CombiChrom min 1B 5 1 min Eluent: A) water/acetic acid (99/1) B) methanol/acetic acid (99/1) 1B CombiChrom can greatly shorten gradient elution analysis time due to a high flow rate. As a result, as many as samples per day can be treated, and the amount of mobile phase used can be reduced. 1. p-hydroxybenzoic acid. n-propyl gallate 3. -Hydroxymethyl-,-di-tert-butylphenol. n-ctyl gallate 5. n-dodecyl gallate 5 mm column Cycle time min CombiChrom Cycle time 7.5 min Possible number of gradient analyses per day 8 times Possible number of gradient analyses per day 19 Amount of mobile phase required for one gradient cycle Analytical column, ml Preparative column, 1 ml (5 mmi.d.) about 1/3 Amount of mobile phase required for one gradient cycle Screen,.5 ml Prep, 5 ml (5 mmi.d.) 98 第章.indd :5:3 AM

7 Scale-up Scale-up to isolation can be achieved easily because the analytical conditions of CombiScreen can be directly applied to CombiPrep. 8 1 min 8 1 min Eluent : A) acetonitrile/water/acetic acid (7/3/1) B) acetonitrile/acetic acid (1/1) -1 B (- min), 1 B (-1 min) 1- B (1-1.1 min), B ( min) Temperature :ambient Detection :UV at 5 nm rdering information YMC CombiChrom Packing material Particle size (µm) CombiScreen. 5 mm CombiPrep 5 mm CombiPrep 3 5 mm Pro C18 S-5 CCASS5-5WT CCASS5-5WT CCASS5-53WT Hydrosphere C18 S-5 CCHSS5-5WT CCHSS5-5WT CCHSS5-53WT Pro C18 RS S-5 CCRSS5-5WT CCRSS5-5WT CCRSS5-53WT DS-A S-5 CCAAS5-5WT CCAAS5-5WT CCAAS5-53WT DS-AM S-5 CCAMS5-5WT CCAMS5-5WT CCAMS5-53WT DS-AQ S-5 CCAQS5-5WT CCAQS5-5WT CCAQS5-53WT DS-AL S-5 CCALS5-5WT CCALS5-5WT CCALS5-53WT Pro C8 S-5 CCSS5-5WT CCSS5-5WT CCSS5-53WT Pro C S-5 CCBSS5-5WT CCBSS5-5WT CCBSS5-53WT Ph S-5 CCPHS5-5WT CCPHS5-5WT CCPHS5-53WT CN S-5 CCCNS5-5WT CCCNS5-5WT CCCNS5-53WT SIL S-5 CCSLS5-5WT CCSLS5-5WT CCSLS5-53WT Polyamine Ⅱ S-5 CCPBS5-5WT CCPBS5-5WT CCPBS5-53WT Diol-NP (1 Å) S-5 CCDNS5-5WT CCDNS5-5WT CCDNS5-53WT 99 第章.indd :5:3 AM

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3 Normal-Phase Columns. Reversed-Phase C18 Columns (ODS) Reversed-Phase Columns (Other than ODS) Columns for HTS and LC/MS Analysis.

3 Normal-Phase Columns. Reversed-Phase C18 Columns (ODS) Reversed-Phase Columns (Other than ODS) Columns for HTS and LC/MS Analysis. We are pleased to present the 11th YMC General Catalog. Since its formation in 1980, YMC Co., Ltd. has been working in the rapidly changing field of chemistry. Over this time, the rate of discovery of