?Who binds to it. ? Who binds these inflammatory proteins RAGE SUGAR-FREE GLYCOBIOLOGY INFLAMMATION. BASIC SCIENCE?sugar chain structure

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1 SUGAR-FREE GLYCOBIOLOGY BASIC SCIENCE?sugar chain structure Unusual Sugar Chain It -- is a carboxylate Make lots of It Make an It antibody Inflammatory proteins?who binds to it? Who binds these inflammatory proteins RAGE INFLAMMATION

2 Expansion, homing and activation of CD4CD45RB hi naive T cells CD4CD45RB hi (naïve) Mesenteric lymph nodes Priming and clonal expansion Involves interaction with DCs IL12 CD8/86-CD28 CD4-CD154 CD134L-CD134 Acquire gut homing molecules α4β7, CCR9 Recognized by addressin molecules on vascular endothelium of intestine and home to lamina propria Th1 Lamina Propria CD4-CD154 CD8/86-CD28 CD134L-CD134 leukocyte recruitment from blood and Inflammation Proinflammatory Cytokines TNFα IFNγ IL-12, IL-23 NO MIF macrophages Apoptotic pathways

3 Expansion, homing and activation of CD4CD45RB hi naive T cells CD4CD45RB hi (naïve) Mesenteric lymph nodes Priming and clonal expansion Involves interaction with DCs IL12 CD8/86-CD28 CD4-CD154 CD134L-CD134 Acquire gut homing molecules α4β7, CCR9 Recognized by addressin molecules on vascular endothelium of intestine and home to lamina propria Th1 Lamina Propria CD4-CD154 CD8/86-CD28 CD134L-CD134 leukocyte recruitment from blood and Inflammation Proinflammatory Cytokines TNFα IFNγ IL-12, IL-23 NO MIF macrophages Apoptotic pathways

4 IL-1 pg/ml IL-12 p4 Secretion of cytokines from LPS-activated RAW264.7 murine macrophages 8 4 TNFα ng/1 6 cells IL12p4 pg/ml TNFα IL-1 untreated LPS LPS+GB3.1 LPS+control Ab

5 mabgb3.1 inhibits LPS-induced TNFα and IL23p19 gene expression in murine macrophages TNF α untreated LPS treated LPS + GB3.1 LPS + control Ab.6.5 IL-23p [-LPS] [+LPS] [+LPS/GB3] [+LPS/cAb]

6 % of total cells in unactivated cultures GB3.1 augments cell death of activated macrophages Time after LPS activation (h) -LPS LPS Activated macrophages +LPS +LPS/cAb +LPS/+GB % of total cells in untreated cultures Unactivated macrophages Time of treatment (h)

7 mabgb3.1 inhibits onset of colitis GB3.1 reduces CD4+ T cell accumulation specifically in colon tissues GB3.1 does not induce significant apoptosis of unactivated or activated T-cells GB3.1 inhibited recruitment of monocytes into inflammed areas or caused apoptosis of recruited macrophages No upregulation of MAdCAM expression Proinflammatory cytokine production is reduced Signaling pathways involved?

8 p65 [LPS activation] p65 [IFN/LPS] NF-kappaB p [unactivated] [activated] [activated/gb3.1] [activated/cab]

9 NF-kappaB p65 is enhanced in colon of Crohn s patients Did GB3.1 block NF-κB in treated mice cells? 1.4 NF-kB levels in colonic lamina propria cells cab Tx GB3 Tx Normal p65 RelB mouse #

10 WILL GB3.1 REVERSE COLITIS? early Day 9 Day21 Leithhauser et al Lab Invest 81, 1339, 21

11 Clinical Symptoms Reconstituted, Untreated/cAb treated Loss of weight, loss of hair, soft stools, ill Reconstituted, GB3.1 treated Minimal weight loss, no hair loss, normal stools, healthy

12 Weight loss curves AJ944 (control B) AJ3524 (Control A) AJ2527 (GB3.1) Days post transfer

13 mabgb3.1 treatment reverses colitis in the emerging phase of disease inflammation: proximal colon no cell transfer cell transfer/untreated cell transfer/cab treated Treatment cell transfer/gb3.1 treated

14 mabgb3.1 treatment reverses colitis in the emerging phase of disease inflammation: distal colon no cell transfer cell transfer/untreated cell transfer/cab treated Treatment cell transfer/gb3.1 treated

15 mabgb3.1 treatment reverses colitis in the emerging phase of disease No cell transfer Reconstituted/ untreated Reconstituted/ cab Treated Reconstituted/ mabgb3.1 treated

16 CONCLUSIONS ANTICARBOXYLATE ANTIBODY GB3.1 BLOCKS THE ONSET OF COLITIS THE ANTIBODY ALSO SEEMS TO REVERSES ESTABLISHED COLITIS EFFECTS ARE PROBABLY MEDIATED BY APOPTOSIS OF ACTIVATED MACROPHAGE GB3.1 MAY BE A POTENTIAL TREATMENT FOR IBD In this collaboration between the Freeze and Kronenberg labs Geetha Srikrishna pioneered these studies and contributed most of the data