Transcription

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24 Farm A (Ingredients %, As fed basis) Farm B (Ingredients%, As fed basis) Beet pulp 10.8 Beet pulp 7.7 Cotton 9.0 Alfalfa bale 7.7 Tall fescue 4.3 Cotton 9.6 Corn silage 18.0 Sudan grass hay 15.3 Timothy hay 9.0 Tall fescue 13.4 Oat hay 2.9 Concentrates 46.0 Rice straw 2.9 By-pass fat 0.1 Alfalfa bale 7.2 Concentrates 36.0 β-carotene 0.1 Total Total Calculated analysis(%, DM basis) Dry matter NEl(Mcal) 1.72 TDN Crude Protein Crude Fiber Ca 0.66 P 0.39 VitA(IU/kg) 84,374 VitD(IU/kg) 2,782 VitE(IU/kg) 16 Calculated analysis(%, DM basis) Dry matter NEl(Mcal) 1.71 TDN Crude Protein Crude Fiber Ca 0.71 P 0.46 VitA(IU/kg) 95,057 VitD(IU/kg) 3,134 VitE(IU/kg) 18

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33 70 IGF-1 content (ng/ ml FarmA FarmB

34 90 IGF-1 content (ng/ ml Early Middle Late (Lactation) Lactation period

35 100 IGF-I(ng/ml Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec Months

36 a a a a

37 O.Dat 280nm kD(catalase) 67kD(albumin) m.w standard Fraction number

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39 60000 Free IGF-I [125 I]-IGF-I cpm/tube (158kD) γ-globulin (44kD) ovalbumin (17kD) myoglobin (1.3kD) vitb Fraction numbers 70

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46 ng/ml Colostrum(prior to heating) after heating Normal(prior to heating) after heating average heating

47 Raw homogenized/uht Product ng/ml Milk Samples Milk whey

48 Raw milk Concentrated 50.0 Dried milk 40.0 ng/ml Whole dried milk

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54 50 IGF-1 content (ng/ml) m 85 15m 3m 0 Raw Milk After homogenization Process Flow After heat treatment After drying After fermentation

55 90 IGF-1 content (ng/ml) Before fermentation After fermentation 0 10% Skim milk 20% dried colostrum fortified medium

56 Dry type Wet type IGF-1 content (ng/ ml ) Storage (d) IGF-1 content (ng/ ml ) Storage (Wks)

57 IGF-1 content (ng/ ml ) Dry type(10.0%, w/w) Wet type(10.0%, v : v) Storage (d) Fortification level was 10% for dry type (w/v). Fortification level was 10% for wet type (v/v).

58 A B C

59 IGF-1 content (ng/ ml ) Prior to fermentation After fermentation Colostrumfortification level (%,w/w) different ratoo of unencapsulated IGF- I. IGF-Ⅰfortified yoghurt with IGF-1 content (ng/ ml Eudragit Prior to fermentation After fermentation Sureteric Coating material Changes of IGF-Ⅰcontent in IGF-Ⅰfortified yoghurt. 10% of total IGF-Ⅰwas substituted with encapsulated IGF- I. Each bar represents the least squares mean ± SE.

60 IGF- I content (ng/ ml ) Prior to fermentation Colostrum Eudragit L-100 Sureteric Storage (d)

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90 A Fraction number

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92 A Fraction number

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95 Cell number ( X10 5 cells/ml) Non-treat. 0.1 ng/ml rhigf-i 1.0 ng/ml rhigf-i 10.0 ng/ml rhigf-i 0.1 ng/ml migf-i 1.0 ng/ml migf-i 10.0 ng/ml migf-i Culture period (day)

96 Cell number ( X10 5 cells/ml) Non-treat. 0.1 ng/ml rhigf-i 1.0 ng/ml rhigf-i 10.0 ng/ml rhigf-i 0.1 ng/ml migf-i 1.0 ng/ml migf-i 10.0 ng/ml migf-i Culture period (day) Cell number ( X10 5 cells/ml) Non-treat. 0.1 ng/ml rhigf-i 1.0 ng/ml rhigf-i 10.0 ng/ml rhigf-i 0.1 ng/ml migf-i 1.0 ng/ml migf-i 10.0 ng/ml migf-i Culture period (day)

97 Cell number ( X10 5 cells/ml) Non-treat. 0.1 ng/ml rhigf-i 1.0 ng/ml rhigf-i 10.0 ng/ml rhigf-i 0.1 ng/ml migf-i 1.0 ng/ml migf-i 10.0 ng/ml migf-i Culture period (day) Cell number ( X10 5 cells/ml) Non-treat. 0.1 ng/ml rhigf-i 1.0 ng/ml rhigf-i 10.0 ng/ml rhigf-i 0.1 ng/ml migf-i 1.0 ng/ml migf-i 10.0 ng/ml migf-i Culture period (day)

98 3 Non-treat. rhigf-i migf-i Absorbance (A450-A655) IGF-I concentration (ng/ml)

99 3 Non-treat. rhigf-i migf-i Absorbance (A450-A655) IGF-I concentration (ng/ml) 3 Non-treat. rhigf-i migf-i Absorbance (A450-A655) IGF-I concentration (ng/ml)

100 3 Non-treat. rhigf-i migf-i Absorbance (A450-A655) IGF-I concentration (ng/ml) 3 Non-treat. rhigf-i migf-i Absorbance (A450-A655) IGF-I concentration (ng/ml)

101 rhigf-i migf-i Growth stimulation ratio (% of control) IGF-I concentration (ng/ml)

102 rhigf-i migf-i Growth stimulation ratio (% of control) IGF-I concentration (ng/ml) rhigf-i migf-i Growth stimulation ratio (% of control) IGF-I concentration (ng/ml)

103 rhigf-i migf-i Growth stimulation ratio (% of control) IGF-I concentration (ng/ml) rhigf-i migf-i Growth stimulation ratio (% of control) IGF-I concentration (ng/ml)

104 28 26 [IGF-I] colostrum Colostrum 24 Body weight (g) Day

105 2.0 [IGF-I] colostrum Colostrum 2.0 [IGF-I] colostrum Colostrum Liver weight (g) 1.0 Muscle weight (g) Day Day 2.0 [IGF-I] colostrum Colostrum 0.5 [IGF-I] colostrum Colostrum Small intestine weight (g) Brain weight (g) Day Day

106 [IGF-I] colostrum Colostrum Serum [IGF-I] (ng/ml) Day

107 18 15 [IGF-I] colostrum Colostrum [IGF-I] colostrum Colostrum Liver [IGF-I] (ng/mg) Muscle [IGF-I] (ng/mg) Day Day Small intestine [IGF-I] (ng/mg) [IGF-I] colostrum Colostrum Brain [IGF-I] (ng/mg) [IGF-I] colostrum Colostrum Day Day

108 60 50 [IGF-I] colostrum Colostrum Serum [IGF-II] (ng/ml) Day

109 30 25 [IGF-I] colostrum Colostrum [IGF-I] colostrum Colostrum Liver [IGF-II] (ng/mg) Muscle [IGF-II] (ng/mg) Day Day Small intestine [IGF-II] (ng/mg) [IGF-I] colostrum Colostrum Brain [IGF-II] (ng/mg) [IGF-I] colostrum Colostrum Day Day

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146 Humbel, 1986) Honegger and

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